• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2006년도 학술발표회 논문집
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• pp.12-17
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• 2006

Natural rivers have water purification functions called Gravel Contact Oxidation Process, which decontaminate river water by biological absorption, oxidation and degradation on riverbed gravels. This function has been developed and applied to many small/medium-sized urban rivers in Japan as one of the direct river water purification methods. However the method hasn't been verified in the tropics, which have different climate conditions and river characteristics. A preliminary experiment carried out at a polluted urban tributary in the outskirts of Kuala Lumpur, Malaysia where an increasing attention has been paid to river environment, showed a good applicability to the tropical conditions as a technically practical water purification measure with some maintenance cares for sludge management.

• 한국농공학회논문집
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• 제56권4호
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• pp.21-28
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• 2014

In this study, evaluated ware the strength and durability of the vegetated water purification channel concrete to which recycled aggregates, hawang-toh and jute were applied. Box-Behnken method of response surface analysis in statistics was applied to the experimental design. Experimental variables are as follows, recycled coarse aggregates, hawang-toh, blast-furnace slag and jute fiber. In the experiment, conducted were the tests of compressive strength, chloride ion penetration, abrasion resistance and impact resistance the replacement rate effects of the recycled aggregates, blast-furnace slag and hwang-toh on the performance of vegetated water purification channel concrete were analyzed by using the response surface analysis method on the basis of the experimental results. In addition, an optimum mixing ratio of vegetated water purification channel concrete was determined by using the experimental results. The optimum mixing ratio was determined to be in 10.0% recycled coarse aggregates, 60.0% blast-furnace slag, 10.1% hwang-toh and 0.16% jute fiber. The compressive strength, chloride ion penetration, abrasion rate, and impact number of fracture test results of the optimum mixing ratio were 24.1 MPa, 999 coulombs, 10.30 g/mm3, and 20 number, respectively.

• 환경영향평가
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• 제18권2호
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• pp.79-87
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• 2009

This study was performed for the application of porous concrete blocks and aquatic plants for the water purification in small urban stream. This study investigated the ability of water purification according to various environments, algae and aquatic plants. When the porous concrete was used as contact media, the average removal efficiencies of SS, BOD and COD were 85~95%, 50~60% and 65~75%, respectively. Also, when the porous concrete and aquatic plants was used the average removal efficiency of SS, BOD and COD were 90~95%, 60~70% and 70~80%, respectively. As the results, average removal efficiency of total nitrogen, at the condition of the porous concrete and aquatic plants, was about 40-50%, then, that of total phosphorus was about 60-70%.

• 한국수산과학회지
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• 제3권1호
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• pp.27-32
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• 1970

살아 있는 개량조개 속에 들어 있는 토사의 배출에 대하여 실험한 결과를 요약하면 다음과 같다. 1) 토사는 주로 입수관 및 출수관 주변, 아가미 주변, mantle주변 그리고 중장선을 위시한 소화 기관 속에 존재하였다. 그리고 소화기관 속에 들어 있는 모래의 크기는 $180\times10\mu\~550\times200\mu$정도의 극히 작은 것들이었다. 2) 시료를 밑바닥에 깔아 놓은 것과 중간층에 수하시킨 것 그리고 단층으로 깔든지 또는 수하시킨 것과 두껍게 깔든지 또는 수하시킨 것 사이에 토사의 배출에 있어 뚜렷한 차이는 찾아 볼 수 없었다. 3) 개량조개는 처음 1시간 정화시키면 약 $50\%$의 토사를 배출하며, $22\~26$ 시간만에 배출물의 양이 거의 일정한 값에 도달하지만, 배출물 속에 토사가 배출되지 않을 때까지는 약 42시간 소요되었다. 4) 토사의 배출에 미치는 PH의 영향을 보면 개량조개가 살고 있는 환경 해수보다 약 1정도 높은 쪽(pH 8.75)이 빨랐다. 5) 토사의 배출에 미치는 온도의 영향은 개량조개가 살고 있는 환경 해수의 온도 때가 온도를 더 높였을 때보다 토사의 배출이 빨랐다. 6) 토사의 배출에 미치는 염분 농도의 영향은 개량조개가 살고 있는 환경 해수의 염분 농도($30.61\%_{\circ}$)보다 약 배로 묽은 쪽($15.14\%_{\circ}$)이 배출이 빨랐다.

• The Plant Pathology Journal
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• 제15권1호
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• pp.34-37
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• 1999

Cymbidium mosaic virus (CyMV) was purified from CyMV infected orchid plant leaves by Sephacryl S-500 HR column chromatography. Partial purification was done by solubilization with Triton X-100 (alkylphenoxypolyethoxy ethanol) and precipitation with polyethylene glycol (PEG 6,000) followed by ultracentrifugation on 30% sucrose cushion. Based on the spectrophotometric analysis, 33 mg of CyMV could be obtained form 100 g of CyMV-infected orchid plant leaves. The purified CyMV represented one distinct homogeneous band by SDS-PAGE, and electron microscopy revealed that it was highly homogeneous and not fragmented. Bioassay demonstrated that the purified CyMV had a normal infectivity to Chenopodium amaranticolor and orchid plants. Based on these results, the purification method in this work could be served as an improved method for the purification of CyMV and similar viruses with good yield, high purity and native integrity.

• Journal of Microbiology and Biotechnology
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• 제19권12호
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• pp.1557-1564
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• 2009

Human CDX2 is known as a caudal-related homeodomain transcription factor that is expressed in the intestinal epithelium and is important in differentiation and maintenance of the intestinal epithelial cells. The caudal-related homeobox proteins bind DNA according to a helix-turn-helix structure, thereby increasing the structural stability of DNA. A cancer-tumor suppressor role for Cdx2 has been shown by a decrease in the level of the expression of Cdx2 in colorectal cancer, but the mechanism of transcriptional regulation has not been examined at the molecular level. We developed a large-scale system for expression of the recombinant, novel CDX2, in Escherichia coli. A highly purified and soluble CDX2 protein was obtained in E. coli strain BL21(DE3)RIL and a hexahistidine fusion system using Ni-NTA affinity column, anion exchange, and gel filtration chromatographies. The identity and secondary structure of the purified CDX2 protein were confirmed by MALDI-TOF MS, Western blot, and a circular dichroism analyses. In addition, we studied the DNA-binding activity of recombinant CDX2 by ELISA experiment and isolated human CDX2-binding proteins derived from rat cells by an immobilized GST-fusion method. Three CDX2-binding proteins were found in the gastric tissue, and those proteins were identified to the homeobox protein Hox-D8, LIM homeobox protein 6, and SMC1L1 protein.

• 대한약학회:학술대회논문집
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• 대한약학회 2001년도 Proceedings of the Pharmaceutical Society of Korea
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• pp.174.3-175
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• 2001

• Advances in environmental research
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• 제9권3호
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• pp.215-232
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• 2020

Crude oils are essential source of energy. It is majorly found in geographical locations beneath the earth's surface and crude oil is the main factor for the economic developments in the world. Natural crude oil contains unrefined petroleum composed of hydrocarbons of various molecular weights and it contains other organic materials like aromatic compounds, sulphur compounds, and many other organic compounds. These hydrocarbons are rapidly getting degraded by biosurfactant producing microorganisms. The present study deals with the isolation, purification, and characterization of biosurfactant producing microorganism from oil-contaminated soil. The ability of the microorganism producing biosurfactant was investigated by well diffusion method, drop collapse test, emulsification test, oil displacement activity, and blue agar plate method. The isolate obtained from the oil contaminated soil was identified as Bacillus subtilis. The identification was done by microscopic examinations and further characterization was done by Biochemical tests and 16SrRNA gene sequencing. Purification of the biosurfactant was performed by simple liquid-liquid extraction, and characterization of extracted biosurfactants was done using Fourier transform infrared spectroscopy (FTIR). The degradation of crude oil upon treatment with the partially purified biosurfactant was analyzed by FTIR spectroscopy and Gas-chromatography mass spectroscopy (GC-MS).

• 통합자연과학논문집
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• 제6권4호
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• pp.193-196
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• 2013

Haemoglobin is an interesting physiologically significant protein composed of specific functional prosthetic haem and globin moieties. In recent decades, there has been substantial interest in attempting to understand the structural basis and functional diversity of avian haemoglobins (Hbs). Towards this end, purification, crystallization, preliminary X-ray diffraction and molecular-replacement studies have been carried out on Amaurornis phoenicurus Hb. Crystals were grown by the hanging drop vapor-diffusion method using PEG 2000 and NaCl as precipitants. The crystals belonged to the primitive monoclinic system $P2_1$, with unit-cell parameters $a=65.33{\AA}$, $b=93.14{\AA}$, $c=98.54{\AA}$, ${\beta}=100.48^{\circ}$; a complete data set was collected to a resolution of $2.6{\AA}$. The Matthews coefficient of $2.30{\AA}^3Da^{-1}$ for the crystal indicated the presence of two ${\alpha}_2{\beta}_2$ tetramers in the asymmetric unit.

• 한국물환경학회지
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• 제20권5호
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• pp.437-446
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• 2004

To develop sewage treatment apparatus by natural purification method, the sewage treatment plant that consisted of aerobic and anaerobic plot was constructed. And then, the effects of treatment conditions on the removal of pollutants in the relation to sewage loading, sewage injection method and season according to the pebble kind. Removal rate of BOD and COD according to the sewage loading in effluent were over 95 and 77%, respectively. Removal rate of nitrogen in treated water by aerobic plot and effluent using sand were about 22~40 and 49~75%, respectively. Those of effluent using sand 75%+ oyster shell 25% and sand 50%+ oyster shell 50% as filter media in comparison with using sand were about 7~25 and 16~23%, respectively. Removal rate of phosphorus in treated water by aerobic plot and effluent using sand as filter media were about 30~36 and 52~56%, respectively. Those of effluent using sand 75%+ oyster shell 25% and sand 50%+ oyster shell 50% in comparison with using sand as filter media were about 11~40 and 12~45%, respectively. Removal efficiency of BOD and COD according to the intermittent injection method of sewage were slightly decreased, but those of nitrogen and phosphorus were little varied in comparison with continuous injection method. Removal efficiency of BOD and COD in winter in comparison with the others were little varied, but those of nitrogen and phosphorus slightly decreased.