• Journal of information and communication convergence engineering
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• 제7권4호
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• pp.576-579
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• 2009

Anti-HIV-1 activities for the extracts (buthanol, hexane, chloroform, and water) of medicinal plants widely used in the folk medicine were evaluated for screening of anti-AIDS agents. The activities of the extracts to inhibit HIV-1 replication were also analyzed. The 50% effective concentration (EC50) of inhibition activity of the p24 production for chloroform extract of Saphora flavescens, chloroform extract of Herba ephedrae, and hexane extract of Pachyma hoelen Rumph showed 5.8, 29.9, and 37.3 2g/ml, respectively, as good activities. Hexane extract of Sophora flavescens, buthanol extract of Tulipa edulis, hexane extracts of Tulipa edulis, Herba ephedra, and Pachyma hoelen Rumph in the 50% cytotoxic concentration ($CC_{50}$) in inhibition activities of recombinant HIV-1 RT showed 12.9, 19.5, 11.6, 12.0, and 36.8 % at concentration of 200 ${\mu}g$/ml, respectively, as good activities. From these results, chloroform extract of Saphora flavescens, chloroform extract of Herba ephedrae, and hexane extract of Pachyma hoelen Rumph were very effective against HIV-1 among all extracts tested. Therefore, we expect these plants will be a useful for anti- HIV-1 therapeutics in future.

• 한국자원식물학회지
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• 제29권5호
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• pp.588-597
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• 2016

Little attention has been paid to the functional aspect of the flower petal of Paeonia lactiflora, compared to that of its root. To determine the components of flower petal of Paeonia lactiflora, we conducted the Fourier transform ion cyclotron resonance (FT-ICR) MASS spectrophotometric analysis. We detected the 24 different types of ingredients from the 70% ethanol extracts of flower petal of peonia lactiflora cv. ‘Red Charm’. The main compounds were quercetin glucopyranosides, methyl gallate, paonioflolol and kaemperol glucopyranosides. We further tested its functional activity. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of the extracts was 87.9-90.4% at 0.1mg/ml. This result showed that these flower extracts have approximately 5-fold stronger antioxidant potential than a previous report with root extracts (Bang et al. 1999). The result of tyrosinase inhibition assay of Paeonia lactflora extract was almost similar to that of arbutin except significantly higher effect in the coral sunset extract at 0.1% concentration. Hyaluronidase inhibition assay showed 76.5% inhibition at 5% concentration of this flower extract, indicating that Peaonia lactiflora flower extracts have the major anti-inflammatory, anti-oxidant and brightening effects. Taken together, these results suggest these three Paeonia lactiflora species extracts might provide the basis to develop a new natural brightening agent.

• 생물환경조절학회지
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• 제31권2호
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• pp.133-141
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• 2022

Bulbophyllum auricomum Lindl.은 희귀 난초이며 매력적인 향기가 지닌 꽃이 피어난다. 본 연구는 미세 증식을 위한 조직 배양 방법을 조사하였다. 인공 자가 수분으로부터 파생된 캡슐은 MS 기본 배지에서 최상의 종자 발아를 위해 얻어진다. 식물 성장 조절제(1.0 mg/L의 BAP 및 2.0 mg/L의 NAA)는 원괴체 유래 유사구근 절편체의 캘러스 유도에 영향을 받았다. 캘러스 계대배양을 위해 10가지 처리 배지에서 다른 천연물 추출물을 테스트하였다. 이 중 150ml/L 코코넛 워터를 첨가한 MS 배지는 일반적으로 1개월 및 2개월에 생중량(1.75 ± 0.08) 및 (3.01 ± 0.20) 캘러스 증식 및 PLB 유도에 효과가 있었고, 그 다음으로 바나나 30g/L와 감자 추출물 20g/L의 MS 조합이 뒤를 이었다. 식물 생장 조절제와 천연 추출물을 함께 첨가한 MS배지와 천연 추출물만 첨가한 MS 배지를 비교 연구한 결과, 천연 추출물(코코넛 워터 150ml/L)과 식물 생장 조절제(BAP 2.0 mg/L 및 NAA 1.0 mg/L)를 혼합하여 MS 배지에 첨가한 결과 배양 1개월 및 2개월 후에 각각 가장 높은 새싹 재생수로 (3.37 ± 0.17) 및 (6.41 ± 0.68)을 얻었다.

• Mycobiology
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• 제47권3호
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• pp.329-334
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• 2019

Streptomyces blastmyceticus strain 12-6 was isolated from a forest soil sample of Cheonan area on the basis of strong antifungal activities against plant pathogenic fungi. Butanol extracts of the cultural filtrates were active against C. acutatum, C. coccodes, C. gloeosporioides, F. oxysporum, and T. roseum. Active fractions were prepared by thin layer chromatography using silica gel plate; 12-6-2 ($R_f$ 0.36), 12-6-3 ($R_f$ 0.44). Scanning electron microscopy showed that the active fractions caused a change in surface texture of fungal spores from smooth surface to wrinkled surface. The lethal effect on the spores of the active fractions varied from 56% to 100%. It was shown that the spores of C. acutatum were more sensitive to the antifungal fractions than the spores of F. oxysporum. Fluorescence staining using TOTO-1 indicated that the antifungal fractions could make the spores more sensitive to the fluorescence dye. Thus, it was suggested that antifungal agents prepared in this study exhibited the antifungal activity by damaging the plasma membrane of both fungal spores and hyphae. Identification of antifungal agents in the active fraction using GC-MS analysis revealed the presence of cyclo-(Leu-Pro) and 9-octadecenamide as major components that have already been known as antifungal substances.

• 동의생리병리학회지
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• 제18권1호
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• pp.250-253
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• 2004

The effects of crude extracts from medicinal plants on biological activity were investigated. The crude ethanol extract of H. paucistipula inhibited the growth of the Gram positive bacterium Bacillus subtilis ATCC 19659, (2 mm inhibition zone at 150 ㎍/disc) and the dermatophyte Trichophyton mentagrophytes ATCC 28185, (7 mm inhibition zone at 150 ㎍/disc), and toxic to P388 murine leukaemia cells ATCC CCL 46 P388D1, (IC/sub 50/ 2.48 ㎍/㎖ at 75 ㎍/disc). This crude ethanol extract of H. paucistipula is the strongest antimicrobial and cytotoxic activities against P388 murine leukaemia cells ATCC CCL 46 P388D1.

• Natural Product Sciences
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• 제27권2호
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• pp.115-121
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• 2021

Lentinus edodes contains functional metabolites such as polysaccharopeptides, lectins, and secondary metabolites. Fermented soybean paste is representative fermented materials in Korea, and is gradually increasing due to various biological activities. In the present study, ethanol extracts of fermented products with/without L. edodes were designated as SPL and SP, and prepared to develop safer and therapeutic functional foods with antioxidant and anti-inflammatory activities for treatment of inflammatory disorders. SP and SPL extracts exhibited antioxidant effects via inhibiting radical activities. Inflammatory mediators, nitric oxide (NO), prostaglandin E₂ (PGE₂), interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor (TNF)-α, and inducible nitric oxide synthase (iNOS) production and nuclear factor-kappa B (NF-κB) activation were down-regulated by two extracts. SPL extract more strongly enhanced the antioxidant and anti-inflammatory activities than SP extract. Its' activities shown more longer fermentation period and more strong inhibitory effects. Taken together, our results suggested that fermented product with medicinal plant has synergic effect and SPL can be a potential candidate for treatment of inflammatory bowel diseases.

• 생명과학회지
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• 제24권5호
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• pp.498-504
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• 2014

HAase는 신체의 연골, 피부, 눈의 초자체 등에 포함된 고분자 다당류의 HA를 분해하는 효소로서 상처나 염증질환 시 활성화되어 염증, 알레르기 유발과 관련된 효소이다. 본 연구는 500종 식물에서 각 종당 한 식물체로부터 한 천연물의 메탄올 추출물(500추출물)을 이용하여 생물학적으로 기능이 다양하고 산업적으로 활용 가능성이 많은 HAase 저해제를 검색하였다. 그 결과 HAase 저해효과가 가장 우수한 천연물 추출물은 때죽나무 줄기 추출물 57.28%, 매화말발도리 줄기 추출물 53.50%의 저해효과를 나타내었다. 이 같은 추출물들의 농도 별 저해효과를 확인한 결과 농도 의존적으로 저해율이 증가하였다. 이 같은 결과로 볼 때 때죽나무, 매화말발도리 추출물은 HAase 효소의 저해효과가 우수하여 향후 HA 분해와 관련된 항염증 및 항 알레르기 연구에 이용될 수 있다.

• 생명과학회지
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• 제19권3호
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• pp.334-342
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• 2009

본 연구는 streptozotocin (STZ)으로 당뇨를 유발한 실험쥐에 대한 Psidium guajava L. 잎(Pg), Lagerstroemia speciosa L. 잎(Ls) 추출물 그리고 혼합물 A (Pg, Ls, Morus indica L. 잎 추출물, Pinus densiflora needles 추출물, Acanthopanax senticosus M. roots 추출물)의 효과를 조사하였다. 4주간 streptozotocin (STZ)으로 당뇨를 유발한 실험쥐에 이들 추출물을 섭취시킨 결과 Pg, Ls 그리고 혼합물 A의 섭취는 당뇨 대조군에 비해 혈당을 각각 20%, 14% 그리고 24% 감소시켰다. 또한 이들 추출물의 섭취는 총 콜레스트롤, 중성지방, 유리지방산을 감소시켰고 인슐린과 HDL-콜레스트롤을 효과적으로 증가시켰다. 결론적으로 혼합물 A는 Pg와 Ls 추출물에 비해 혈당 및 지질 감소, 인슐린 증가 효과 그리고 기능적으로 손상된 베타세포의 회복이 더 높은 것으로 나타났다. 따라서 혼합물 A는 개별적으로 사용한 Pg와 Ls 추출물에 비해 더욱 유익한 상승효과를 발휘하는 것으로 증명되었다.

• 한국한의학연구원논문집
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• 제16권3호
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• pp.167-173
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• 2010

Objects : Glycine max Merr. (black bean), Triticum aestivum L. (wheat) and Oryza sativa L. (rice bran) have been widely used for treatment of relaxion of smooth muscle, gastrointestinal hemorrhage and alopecia in Korean Traditional Medicine. In this research, we examined the effect of the extracts, obtained from EtOH extracts of 3 kinds of traditional plants, on hair growing activity of the DP6 and C3H10T1 cell and physical properties. Materials and Methods : On the basis of previous studies, three traditional plants were selected and we extracted them with ethanol. We evaluated their hairy dermal papillar cell proliferation activity and mouse mesenchymal stem cell in vitro model. Also, 3 herbal extracts were added to the normal shampoo formulation in ranges of 0.1% and we validated tensile properties and physical changes using aged hair. In this research, we compared the tensile strength, shine and color appearance between the hair (general formulation) and the hair after applying shampoo with natural extracts. To analyze the luster and color image, we use the SAMBA hardware and software made by Bossa Nova Technologies. Results : In the comparative test for tensile characteristic between the hair treated general formulation(control) and the hair applying special formulation including 3 kinds of extracts, tensile distance and energy of the latter are larger than control on average. The shine and color appearance were also increased after using shampoo including natural extracts(shine : 10.9%, color appearance: 24.12%). We observed the enhancement of hair growth activity in the DP6 and C3H10T1 cell. Especially black bean extracts had the most powerful effect in the dermal papillar cell proliferation. Conclusion : These experiments suggest that extracts of Glycine max Merr. (black bean), Triticum aestivum L. (wheat) and Oryza sativa L. (rice bran) stimulate the hair growth activity and can improve physical activities of aged hair. Shampoo product, which contains 3 kinds of natural extracts, would be used for the treatment for aged hair.

• Journal of the Korean Wood Science and Technology
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• 제49권4호
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• pp.384-393
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• 2021

Recently, due to environmental and toxicity issues, there has been increasing attention on research regarding natural products that can reduce the use of chemical fertilizers. Wood extracts derived from the biorefining process contain various fertilizer ingredients. HPLC analysis revealed that wood extract contains approximately 5.2% hemicellulosic sugar. The growth of lettuce (Lactuca sativa) upon treatment with wood extract (extract obtained from steam-exploded pine) or water-soluble fertilizers containing different nutrients was analyzed in this study. After two weeks, the growth characteristics of lettuce as affected by wood extract or water-soluble fertilizers were significantly different. The effect of water-soluble fertilizers containing ascorbic acid, magnesium sulfate, citric acid, potassium nitrate, amino acids, or seaweed extract was less desirable than that of wood extracts regarding plant height (18.6 cm), number of leaves (10), leaf length (14.1 cm), shoot fresh wight (9.8 g/plant), root fresh weight (0.8 g/plant) and shoot dry weight (0.6 g/plant). The plant height, number of leaves, leaf length, shoot fresh wight, root fresh weight, shoot dry weight of water-soluble fertilizers containing wood extract were significantly different compared to the control (plant height :13.5 cm, number of leaves : 7, leaf length : 9.4 cm, shoot fresh wight : 5.3 g/plant, root fresh weight : 0.7 g/plant, shoot dry weight : 0.4 g/plant, root dry weight : 0.07 g/plant). From these results, it was concluded that wood extract can be used as a potential water-soluble fertilizer to increase the yield of leafy vegetables.