• 동아시아식생활학회지
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• 제25권1호
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• pp.87-98
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• 2015

We investigated the apoptotic effects of grape skin extracts (GSE) and related gene expressions in human breast cancer MDA-MB-231 cells cultured in the presence of 0, 0.5, 1 and 1.5 mg/mL of GSE for 72 hours. MTT assay, trypan blue and nuclei staining showed lower cellular mitochondrial activities and increased cell deaths with a higher concentration of GSE (p<0.05). Increased cell number with fragmentated DNA of sub-G1 phase was calculated as a measure of apoptotic cell death by FACS analysis (p<0.05). In particular, apoptotic cell death caused markedly increased in the 1 and 1.5 mg/mL of GSE groups, as revealed by flow cytometry (Annexin V-FITC). RT-PCR analysis was performed on apoptotic and preapoptotic genes. Expression of the apoptosis suppressor gene bcl-2 significantly decreased, proapoptotic gene bax was significantly increased and procaspase-3 showing the presence of caspase-3 significantly decreased (p<0.05). Furthermore, bcl-2/bax ratio which is considered to be an important indicator of apoptosis, significantly decreased in a concentration-dependent manner (p<0.05). These results indicated that GSE induces apoptosis in MDA-MB-231 human breast cancer cells.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2004년도 춘계학술대회논문집
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• pp.870-875
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• 2004

Beam stiffeners have frequently been used for raising natural frequencies of base structures. In stiffener layout optimization problems, most of the previous researches considering the position and/or the length of the stiffener as design variables dealt with structures having just simple convex shapes such as a square or rectangle. The reason is concave shape structures have difficulties ill formulating geometry constraints. In this paper, a new geometry constraint handling technique, which can define both convex and concave feasible lesions and measure a degree of geometry constraint violation, is proposed. Evolution strategies (ESs) is utilized as an optimization tool. In addition, the constraint-handling technique of EVOSLINOC (EVOlution Strategy for scalar optimization with Lineal and Nonlinear Constraints) is utilized to solve constrained optimization problems. From a numerical example, the proposed geometry constraint handling technique is verified and proves that the technique can easily be applied to structures in net only convex but also concave shapes, even with a protrusion or interior holes.

• 한국소음진동공학회논문집
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• 제14권5호
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• pp.410-415
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• 2004

To ensure a safe operation of the prototype KGT-74 kW small gas turbine, vibrational reliabilities of the compressor 1st, 2nd, and 3rd stages and turbine blades have been estimated and reviewed. FE analyses have been carried out to obtain the natural vibration characteristics of the blades, and impact modal testings have been performed on every each one of the blades to measure their 1st natural frequencies. Then, the Campbell diagram analyses have been carried out to judge the safety of the blades from resonant failures up to 6k harmonics. Results show that the compressor 1st stage blade is exposed to a potential resonant failure with 3k harmonic around a rated speed of 30,000 rpm but that the other compressor 2nd and 3rd stages and turbine blades are safe from resonant failures. Finally, 27,900 rpm is selected as the safe operation limit for the KGT-74 ㎾ gas turbine relative to the blade vibrations.

• 한국방사선학회논문지
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• 제5권6호
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• pp.351-355
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• 2011

본 논문은 재난재해와 방사선 오염으로부터 안전한 농작물 관리를 위한 플랫폼, 그리고 식물 생장 모니터링 시스템을 제안하였다. 또한, 식물생장을 모니터링하여 식물공장 내에서 생장하는 식물의 크기를 효율적으로 측정할 수 있는 시스템을 개발하였다. 본 연구에 따른 기대효과는 첫째, 자연재해와 방사능오염 감시를 통해 신속하고 정확한 대처 기능으로 농산물 품질과 생산성 향상을 가져올 수 있다. 둘째, 식물의 크기 측정 데이터를 유지 관리하는데 소요되는 시간을 절약하여 경비를 절감할 수 있다. 마지막으로 식물 공장 관리자의 작업 효율을 향상시킬 수 있다.

• 한국작물학회지
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• 제44권1호
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• pp.78-85
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• 1999

To investigate kernel hardness, a compression test which is widely used to measure the hardness of individual kernels as a physical testing method was made simultaneously with the measurement of friabilin (15KDa) which is strongly associated with kernel hardness and was recently developed as a biochemical marker for evaluating kernel hardness in 79 Korean wheat varieties and experimental lines. With the scattered diagram based on the principal component analysis from the parameters of the compression test, 79 Korean wheat varieties were classified into three groups based on the principal component analysis. Since conventional methods required large amount of flour samples for analysis of friabilin due to the relatively small amount of friabilin in wheat kernels, those methods had limitations for quality prediction in wheat breeding programs. An extraction of friabilin from the starch of a single kernel through cesium chloride gradient centrifugation was successful in this experiment. Among 79 Korean wheat varieties and experimental lines 50 lines (63.3%) exhibited a friabilin band and 29 lines (36.7%) did not show a friabilin band. In this study, lines that contained high maximum force and the lower ratio of minimum force to maximum force showed the absence of the friabilin band. Identification of friabilin, which is the product of a major gene, could be applied in the screening procedures of kernel hardness. The single kernel analysis system for friabilin was found to be an easy, simple and effective screening method for early generation materials in a wheat breeding program for quality improvement.

• 한국의류학회지
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• 제41권3호
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• pp.527-536
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• 2017

This study investigated the detergency and functionality of laminating finished fabrics for outdoor wear based on repeated washing. Laminating finished fabrics were selected as the main fabrics for outdoor wear and used as test fabrics. The effects of outdoor exclusive detergent and normal neutral detergent were examined according to washing time, temperature, rpm and detergent concentration based on the use of a Terg-O-Tometer. Re-soiling of the test fabrics was measured by Florio-Mersereau. Permeability, water repellency, water resistance and absorbency were estimated to measure improvements and effects in regards to outdoor exclusive detergent in optimal washing conditions. The detergent effect of outdoor exclusive detergent was superior compared to normal neutral detergent. Re-soiling was lower with exclusive outdoor detergent than with normal neutral detergent. The measurement of functionality for laminating finished fabrics before and after washing indicated that functionality was decreased with repeated washing.

• 한국정밀공학회지
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• 제26권4호
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• pp.107-113
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• 2009

In this paper, we studied of measurement the vibration of natural frequency using optical fiber sensor. The boring bar for measurement of vibration in use optical fiber sensor has the advantage of direct measure for the frequency than accelerometer. Because it deal with output value on electrical signal of optical fiber in physical disturbance when it measures the frequency of vibration. The optical fiber sensor measured the vibration of boring bar by the gap in sensing jig while optical fiber just kept contact with boring bar. A prototype system was composed of jig part with gap and optical system part. In this paper, we found out the possibility to measurement of vibration by the gap in use optical fiber.

• 한국환경복원기술학회지
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• 제6권5호
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• pp.28-39
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• 2003

This study was carried out to elucidate effective restoration measures for natural forest with recycling native tree un site from November 2001 to October 2002 to obtain a basic information for revegetation measure, eight experimental treatment was done and the length of stump, root-ball size of stump, antisepsis treatment of trunk cut, Planting season and contents of organic matter in soil were effective on regrowth of root-stock. Thirteen tree species including Quercus acutissima among twenty tree species showed outstanding sprout and survival rate(over 90 percent), Planting in November and combinated planting with 5 trees and 9 shrubs of root-stock per 100$m^2$ plot showed a good growth. And 10 percent of organic matter plot showed a good crown coverage.

• 한국정밀공학회지
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• 제16권5호통권98호
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• pp.11-18
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• 1999

Recently high speed machining is being studied actively to reduce machining time and to improve machining precision. To perform efficient high speed machining, evaluation of high speed machinability must be studied preferentially and it can be identified by investigation of cutting force. To measure cutting forces in high speed machining, dynamometer which has high natural frequency was newly designed using 3-axes piezo force sensor. For newly designed dynamometer, calibration is conducted with sensitivity of force sensor modulated and proper preload and interference force are investigated experimently. Also, cutting force signals of newly designed dynamometer are compared with those of conventional one in high speed cutting experiment and its superiority is confirmed. Then using newly designed dynamometer, high speed machinability is evaluated about cutting force and tool wear in various cutting conditions.

• 한국환경농학회지
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• 제39권2호
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• pp.89-94
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• 2020

BACKGROUND: Persistent organic contaminants such as dichlorodiphenyltrichloroethane (DDT) are often found in agricultural soils decades after it was banned in Korea. This study uses hemoglobin and hemoglobin-containing blood meal to reduce the residual DDT in soil. METHODS AND RESULTS: Hemoglobin or blood meal with or without hydrogen peroxide (H₂O₂) was mixed with the DDT-spiked soil prepared for this study, and samples were taken over 14 d-degradation period to measure the residual DDT concentrations. With only hemoglobin, DDT was completely removed after 14 d, while with both hemoglobin and H₂O₂, 73%, on average, removal was observed. Similarly, the blood meal removed 73% of DDT, but with H₂O₂, the DDT removal was only 39%. The lower DDT removal in the presence of H₂O₂ can be attributed to the adverse effects of reactive species. Hemoglobin was more effective than blood meal for DDT removal in a given time; however, with additional blood meal injection, complete DDT removal was achieved. CONCLUSION: Overall, this study shows that the blood meal that is used as a fertilizer can potentially be used to remove residual contaminants such as DDT in agricultural soil.