• Applied Microscopy
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• 제28권3호
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• pp.329-343
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• 1998

Early wound healing responses in the skin of the Korean fire bellied toad, Bombina orientalis, were examined using histochemical , scanning and transmission electron microscopical techniques. Cutaneous wounds were induced by sharp razor blade , and maintained in special cages for up to 3 days. By this treatment whole parts of epithelium and dermis were profoundly affected. After injury, retraction of damaged surface of the skin was observed. At 3 hours after injury, poly-band was form by mucous substance. The wound skin consisted of mucous gland secreted mucous substance which flows into wound cavity and the wound area was sealed by mucous substance which form thick layer at 6 hours. It appeared that natural disposition showed acid and neutral mucous substance by reaction of PAS and Alcian blue (pH 2.5) staining. After formation of the poly-band, epithelial cells were formed by migration of regenerating epithelial cells. These cells moved over the wound surface by migration at 12 hours after wounding. Poly-band was much increased in thickness and migrating cells were completely formed wound closure at 24 hours post-wounding. After 72 hours regenerated epithelial layer consists of four or five cell layers which are similar to clear, granular, and spinous layer.

• 농촌계획
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• 제5권2호
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• pp.46-55
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• 1999

This paper examines the pastoral ideal and its legacy to landscape design. We have had the routine convention of experiencing natural beauty from the picture-like nature. We, who would liken a scenic view to a picture, often equate natural beauty with superficial representations of nature shown in pastoral literature and traditional landscape paintings: the lush of towering trees, the field of endless green, the soft babbling flow of the river and the crisp clear sky. It is not a portrayal of nature as it is, but in fact a conceptual expression of its ideal form. The ideological root of that natural beauty can be retraced to the pastoral ideal, so eagerly longed for by people of the western world. A pastoral landscape graciously marked with leisurely peace and subtle harmony is what we identify as beautiful nature. In fact, however, it is no more than an artificial refuge in contextual isolation from its surrounding, and a by-stander's nature seen purely through the eyes of the outsider. The tradition of pastoralism, reaching its peak in the 18th century with its English Landscape Garden style, was transplanted into the real world through the practice of landscape architecture. Landscape design in such a form is just a static means of decoration devoid of meaning and process. And we simply identify the green ornamentation with nature.

• Journal of Microbiology
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• 제38권2호
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• pp.88-92
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• 2000

A human ribosomal protein S3 (rpS3), which also functions as a DNA repair enzyme(UV endonuclease III), was expressed in a methylotrophic yeast, Pichia pastoris, and biochemically characterized. UV endonuclease activity was preiously characterized, and this activity of mammalian rpS3 was found to be non-specfic upon purification and storage. Under the Pichia expression system, the subcloned cDNA of the human rpS3 gene revealed a peptide of 42 kDa by SDS-PAGE and Western blot. The secreted form of human rpS3 rendered no endonuclease activity while the intracellular form showed UV specific endonuclease activity by the nick circle assay.

• Animal cells and systems
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• 제4권4호
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• pp.381-388
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• 2000

Using a murine T cell hybridoma, activation-induced cell death (AICD) was studied. As an in vitro model system for the AICD, 1 cell hybridoma expressing TCR/CD3 complex was incubated onto the immobilized purified anti-CD3 antibody. The immobilized anti-CD3 antibody induced AICD effectively up to 40%. At 1-100 $\mu$M range of SNP, an exogenous source of nitric oxide (NO), the cell proliferation was not affected, but at 1 mM SNP, cell proliferation was significantly reduced. The AICD of T cell hybridoma was inhibited by exogenous NO at non-cytotoxic concentration, In the cells undergoing AICD, the expressions of caspase-3 and FasL were detected, but not iNOS. Similar result was recognized in the apoptosis induced by dexamethasone, an apoptosis-inducing agent. However, the conversion from the inactive form of caspase-3 (32 kDa) to the active form (17 kDa) was significantly reduced in the cells in AICD induced by anti-CD3 antibody, With the result of increased PARP cleavage in the cells, we propose that another PARP cleavage pathway not involving caspase-3 may function in the anti-CD3 antibody induced AICD in the T cell hybridoma.

• IMMUNE NETWORK
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• 제16권5호
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• pp.305-310
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• 2016

In this study, we compared two different tumor cell vaccines for their induction of anti-tumor immunity; one was a tumor cell clone expressing a membrane-bound form of IL-12 p35 subunit (mbIL-12 p35 tumor clone), and the other was a tumor clone expressing heterodimeric IL-12 as a single chain (mb-scIL-12 tumor clone). The stimulatory effect of mb-scIL-12 on the proliferation of ConA-activated splenocytes was higher than that of mbIL-12 p35 in vitro. However, the stimulatory effect of mbIL-12 p35 was equivalent to that of recombinant soluble IL-12 (3 ng/ml). Interestingly, both tumor clones (mbIL-12 p35 and mb-scIL-12) showed similar tumorigenicity and induction of systemic anti-tumor immunity in vivo, suggesting that tumor cell expression of the membrane-bound p35 subunit is sufficient to induce anti-tumor immunity in our tumor vaccine model.

• Journal of Forest and Environmental Science
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• 제15권1호
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• pp.1-9
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• 1999

천연활엽수림에서 자생하는 활엽수종 고유의 생장 특성을 감안하여, 18개 주요 상층 임관 구성 수종을 대상으로 임목 형질을 평가하는 한 방편으로서 임목(林木) 형질(形質) 지수(指數)(tree form index: TFI)를 추정하였다. 선정된 6개의 형질 속성을 4개의 급수로 나누고, 임목 개체의 전반적인 형질에 미치는 영향력을 고려하여 합당한 점수를 부여하였다. 18개 수종에 대하여 속성 별로 점수를 부여하고 백분률(百分率)로 환산한 후, 각 점수를 합하여 임목 형질 지수를 산출하였다. 각 쌍의 수종별 점수 차이와 $18{\times}6$ 행렬(行列)을 바탕으로 집락분석(集落分析)(cluster analysis) 을 시도하여 수종별로 어떠한 속성들이 공통적인가를 검토하였다. 연구 결과, 임목 형질이 가장 좋은 수종은 TFI 수치 80.8을 기록한 사시나무로 파악되었고, TFI 수치 46.3을 기록한 까치박달나무는 임목 형질이 가장 좋지 않은 수종으로 분석되었다. 70점 이상의 비교적 높은 지수를 획득한 상층 임관 수종들은 사시나무, 거제수나무, 들메나무, 느릅나무 등으로서, 통직하고 완만한 수간, 높은 지하고(枝下高), 좁은 수관(樹冠), 가는 가지 등의 특성을 갖는 장점을 지니고 있었다. 반면에, 60점 미만의 낮은 지수를 얻은 수종들은 팔배나무, 산벚나무, 복장나무, 가래나무, 까치박달나무 등으로서, 굽었거나 기울어진 수간, 낮은 지하고(枝下高), 넓은 수관, 굵은 가지 등의 성격을 갖는 경향이 있었다. 집락분석 결과에 의한 분류도(分類圖)에서 Euclidean 거리계수(距離係數) 임의의 값 10은 연구 대상 수종들을 4개의 뚜렷한 수종군(樹種群)으로 분류하였고, 각 수종군에 대한 임목 형질 특성을 검토하였다.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2005년도 춘계학술대회
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• pp.609-614
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• 2005

Gas hydrates are ice-l ike sol id compounds that are composed of water and natural gas. All common gas hydrates belong to the three crystal structures that are composed of five polyhedral cavities formed by hydrogen bonded water molecules and stable in specific high pressure and low temperature conditions. Gas hydrates contain large amounts of organic carbon and widely occur in deep oceans and permafrost regions, and they may therefore represent a potential energy resource in the future. United States and Japan perform the national R&D programs for the commercial production of gas hydrates in 2010's. The study on gas hydrates are also important for exploration and development of natural gas in the regions where gas hydrates are accumulated and could be formed. Although their global abundance is debated, they play an important role in global climate change since methane is a 50 times more effect ive greenhouse gas than carbon dioxide. Natural gas hydrates also form a possible natural hazard if rapidly dissociated and can cause slides and slumps and in the marine environment associated tsunamis.

• 한국실내디자인학회논문집
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• 제1호
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• pp.72-75
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• 1992

Lighting made interior space visible and to recognize space itself. Sp9ace and form are established not themselves , but lighting. and dextrous treatment of natural light accomplished poeteic space. Inflowing of lighting in interior space is main factor to decide space character and image . That was recognized in interior space through many centries. At this point, this study was described for that present inflowing of lighting adapting space character and right method in lighting program. The present study is to present the planning method of the most appropriate and the most rational natural lighting in planing for lighting. Under this premise the followings are made ; I. An introduction , the purpose, the sphere, and the method of this study are stated. II, The present writer studied the general characteristics of natural lighting. III. this author studied the factor of decision on control and representation skill of lighting along the following. IV. Analyzing interior space in which the natural lighting is introduced through some literature and case studies. Physics, psychology, structure, equipment, material. V. The last, the author rearranged the results of this study.

• 대한약학회:학술대회논문집
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• 대한약학회 2001년도 Proceedings of the Pharmaceutical Society of Korea
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• pp.90-94
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• 2001

Wondonins A and B, aromatic alkaloids of an unprecedented skeletal class have been isolated form and association of the sponges Poecillastra wondoensis and Jaspis sp. In addition, four novel bromotyrosine-derived metabolites, psammaplins $A_1$ and $A_2$, aplysinellins A and B, have been isolated from the tropical sponge Aplysinella rhax. The structures of these compounds have been determined on the basis of combined chemical and spectral analyses. The new compounds exhibited significant cytotoxicity and antiangiogenic activity as well as inhibitory activities against farnesyl protein transferase and leucine aminopeptidase. In addition to these compounds, several bioactive metabolites have been isolated from sponges of Korean and tropical waters.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2002년도 ITC-CSCC -2
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• pp.826-829
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• 2002

This system is unionized form about CPS and a pocket game machine. This game machine of the use only for the game with an existing system but the function of various purposes which carries out service relevant to a user's position information. This system have the game function, the function to offer space guidance service by the theme interlocked with a user's position, and the traffic safety education function.