• Phonetics and Speech Sciences
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• v.7 no.3
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• pp.99-105
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• 2015

The current study investigates whether Korean-speaking adults show differential sensitivities to the sources of auditory stimuli (L1 Korean and L2 English) in utilizing VOT and f0 in the perceptual mode of L2 stops, and how the L2 proficiency interacts with the learners' low-level phonetic sensitivities in L2 perceptual mode. 48 Korean learners of English participated in the perception experiments where they rated the goodness of English /t/ and /d/ using an analogue scale. Two sets of stimuli (English and Korean sources) were prepared by manipulating VOT (6-steps) and f0 (5-steps) values of productions by an English male (L2 source condition) and a Korean male (L1 source condition). Findings showed that, in judging /t/-likeness, the listeners responded differently to the two auditory stimulus conditions by relying on VOT significantly more in English source condition than in Korean source condition. The listeners' English proficiency did not interact with these differential sensitivities to the auditory stimulus source either along the VOT dimension or the f0 dimension. The results of the current study suggest that low-level contextual information of the auditory source can affect the learners in faithfully being in the L2 perceptual mode.

• International Journal of Industrial Entomology and Biomaterials
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• v.45 no.2
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• pp.56-69
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• 2022

Silk is a unique natural biopolymer with outstanding biocompatibility, high mechanical strength, and superior optical transparency. Due to its excellent properties, silk has been widely reported as an ideal biomaterial for several biomedical applications. Recently, fluorescent silk protein, a variant of native silk, has been reported as a biophotonic material with the potential for bioimaging and biosensing. Despite the realization of fluorescent silk, the traditional degumming process of fluorescence silk is crude and often results in fluorescence loss. The loss of fluorescent properties is attributed to the sensitivity of silk fibroin to temperature and solvent concentration during degumming. However, there is no comprehensive information on the influence of these processing parameters on fluorescence evolution and decay during fluorescent silk processing. Therefore, we conducted a spectroscopic study on fluorescence decay as a function of temperature, concentration, and duration for fluorescent silk cocoon degumming. Sodium carbonate solution was tested for degumming the fluorescent silk cocoons with different concentrations and temperatures; also, sodium carbonate solution is combined with Alcalase enzyme and triton x-100 to find optimal degumming conditions. Additionally, we conducted a molecular dynamics study to investigate the fundamental effect of temperature on the stability of the fluorescent protein. We observed degumming temperature as the prime source of fluorescent intensity reduction. From the MD study, fluorescence degradation originated from the thermal agitation of fluorescent protein Cα atoms and fluctuations of amino acid residues located in the chromophore region. Overall, degumming fluorescent silk with sodium carbonate and Alcalase enzyme solution at 25 ℃ preserved fluorescence.