• BMB Reports
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• 제29권2호
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• pp.116-121
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• 1996

In earlier studies, the genes encoding Escherichia coli thioredoxin and Corynebacterium nephridii thioredoxin C-3 were fused via a common restriction site in the nucleotide sequence coding for the active site of the proteins to generate two chimeric thioredoxins, designated E-C3 (N to C-terminal) and C3-E. The hybrid thioredoxins were overexpressed in E. coli from the cloned chimeric thioredoxin genes by a T7 promoter/polymerase system. To investigate the structure-function relationship of thioredoxin, we purified the E-C3 hybrid thioredoxin through ammonium sulfate fractionation, DEAE-cellulose chromatography, and Sephadex G-50 gel filtration. Its purity was examined on SDS-polyacrylamide gel electrophoresis and the molecular weight of the purified E-C3 hybrid thioredoxin was estimated to be 12,000. On native polyacrylamide gels, the purified E-C3 hybrid thioredoxin shows a much lower mobility than E. coli thioredoxin. E-C3 hybrid thioredoxin exhibits a 40-fold lower catalytic efficiency with E. coli thioredoxin reductase than E. coli thioredoxin. It was shown to catalyze the reduction of insulin disulfide by dithiothreitol. The purified E-C3 hybrid thioredoxin was also characterized in other aspects.

• 한국자원식물학회지
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• 제22권4호
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• pp.312-316
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• 2009

In the present study, the anti-cancer activity of 80% ethanol extracts from 120 kinds of medicinal herbs and native plants were investigated. Among them, the barks of Melia azedarach L. var. japonica Makino showed the highest cytotoxicity in HCT-15 human colon cancer cell. With this result, we carried out hollow fiber (HF) assay and anti-metastasis study to confirm the anti-cancer effects of M. azedarach var. japonica. In MTT assay, M. azedarach var. japonica.inhibited the proliferation of HCT-15 cells in dose-dependent manner. HF assay was carried out using A549 human adenocarcinoma cell, HCT-15 and SK-Hep1 human liver cancer cell via intraperitoneal (IP) and subcutaneous (SC) site. As a results, SK-Hep1 implanted in IP site showed the highest cytotoxicity. The result from metastatic model using B16/BL6 mouse corresponded to that of HF assay. These results suggest that the ethanol extract from M. azedarach var. japonica. might have a potent anti-cancer activity and advanced study is needed for the development of novel natural anti-cancer drug.

• 한국조경학회지
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• 제35권1호
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• pp.9-19
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• 2007

This plan was submitted as part of an invited competition for the provincial park planning of Yeonin Mountain, which was held by the Gyeonggi Innovation Corporation in November, 2006. The proposed site is located at Seungan-ri, Gapyung-eup, Gapyung-gun, Gyunggi-do and covers about $150,010m^2$. The main goal of this project is to "Suggest a New Park Paradigm" by shedding old ways of thinking about parks through an integrated development concept. Planning was approached as follows: First, providing an unique theme to the support facilities area of Yeonin Mountain Piovincial Park. To achieve this theme, the Seungan support facilities area was designated to be home to a Seven Colored Theme Garden, currently named the Native Wild-flower Garden, while the Baekdun facilities area is to be centered on a Self-loaming Forest Camp, which will give the chance of a hands-on wildlife experience. With these themes, which create recreational goals that will encourage not only tourism but education as well, the foundation was laid for a multi-purpose park paradigm. Second, Developing a Core Facility Zone. To accomplish the development of a core facility zone, park buildings will be arranged in accordance with an integrated building lay-out for easy access and use, and the facilities specified by design for the Seven Colored Theme Garden are provided for the newly prepared site. This will allow focus on the user's viewpoint instead of the view of the planner or of ease of maintenance.

• KIEAE Journal
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• 제15권4호
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• pp.13-20
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• 2015

Purpose: Pungsu is can be seen as an ecological architectural science regarding native natural environment of east asia. Even though the language used in Pungsu is different from that of ecological architecture, Pungsu considered the surrounding environment of the traditional house by various Pungsu method for making and keeping more healthy life and sustainable environment. The research is for finding out how the surrounding natural environment was considered with Pungsu, a traditional ecological architectural science in case of site selection and planning house by the confucian scholar in late Joseon period. Method: Sudan Nam-Gyu Lee's traditional house of Yesan in the middle of korea is selected. The study is processed as follows. The site was analyzed with feature and flow of mountains expressed as dragon in chapter 2 and four important hills around the house are analyzed in chapter 3. Finally Pungsu applied in architectural space is analyzed in chapter 4. Result: we can see that the traditional house of Sudang Lee Nam-Gyu has been planned considering Pungsu in details to minimize the negative factors and utilize the positive factors of nature as much as possible thinking nearby natural environment as environmental factors that can affect the residents of the house through Pungsu.

• 한국세라믹학회지
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• 제47권1호
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• pp.19-25
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• 2010

Traditional Kr$\ddot{o}$ger-Vink (K-V) notation defines sites in ionic crystals as interstitial or belonging to host ions. It enables description and calculations of combinations of native and foreign defects, including dopants and substituents. However, some materials exhibit inherently disordered partial occupancy of ions and vacancies, or partial occupancy of two types of ions. For instance, the high temperature disordered phases of $Bi_2O_3$, $Ba_2In_2O_5$, $La_2Mo_2O_9$, mayenite $Ca_{12}Al_{14}O_{33}$, AgI, and $CsHSO_4$ are all good ionic conductors and thus obviously contain charged point defects. But traditional K-V notation cannot account for a charge compensating defect in each case, without resorting to terms like "100% substitution" or "Frenkel disorder". the former arbitrary and awkward and the latter inappropriate. Instead, a K-V compatible nomenclature in which the partially occupied site is defined as the perfect site, has been proposed. I here introduce it thoroughly and provide a number of examples.

• BMB Reports
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• 제41권4호
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• pp.305-309
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• 2008

Cobalt is an essential microelements in many biological processes involving enzymatic activity. We found that $Zn^{2+}$ and $Mg^{2+}$, which are in the active site of native calf intestine alkaline phosphatase (CIP), can be replaced by $Co^{2+}$ directly in solution. The effect of $Co^{2+}$ concentration on the substitution reaction was examined at ratios of [$Co^{2+}$]/[CIP] from 0:1 to 8:1. The quantity of $Zn^{2+}$ in CIP decreased progressively as the ratio was increased, but the amount of $Mg^{2+}$ changed in irrregular fashion. A series of active site models of the reaction mechanism of CIP are proposed. Low pH was found to promote the replacement of $Mg^{2+}$ by $Co^{2+}$. To understand how the substitution affects the enzyme, we also solved the secondary structure of CIP after reaction with $Co^{2+}$ in different conditions.

• 한국환경복원기술학회지
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• 제12권6호
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• pp.51-62
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• 2009

The purpose of this study is to analyze the profitability by selecting Pinus densiflora S. et Z. worthy of ornamental tree from among the cutting timber in the urban development project, and then presents the improvements. The study site is the whole area of Oedong-eup Gyeongju city Gyeongsangbuk-do and subject trees numbers are 2,180 trees. Analysis method is cost analysis because it is used generously pubic land development. In results, at first, in case of forest development currently cutting tree costs 7.3 million Won/ha. and no profit is occurred at all. Secondly, the forward direction order of tree treatments expenses are in sawdust production, selling lumber, selling ornamental tree, and selling ornamental tree, sawdust production, selling lumbered on the aspect of profits by the selling stumpage. Especially, selling trees as ornamental tree costs 127 million Won/ha. which shows the highest profits. Third, the management of ornamental tree pine tree after transplantation from development site costs 5 times less than purchasing the same quality trees. Hence, it is recommended to re-utilize the trees from the development sites to produce a high standard gardens and it also provides the basis for the low carbon green growth society.

• The Korean Journal of Pain
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• 제6권2호
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• pp.237-246
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• 1993

세포막에서 마취제의 작용점을 규명하기 위하여, 마취제의 많은 부분을 차지하는 n-Alkanol을 이용하여, 소의 synaptosomal plasma membrane vesicles(SPMV)에서 n-Alkanol의 침투 정도를 형광 probe를 이용한 형광소광법을 통하여 검색하였다. n-Alkanols는 SPMV 외부 단층(outer monolayer)의 표면에 주로 분포하되 그 탄소수에 비례하여 소수성 부위에 분포되는 양이 증가되는 경향을 나타내었다(1-decanol은 제외). Methanol, Ethanol, 1-propanol, 1-butanol, 1-pentano, 1-hexanol, 1-heptanol, 1-octanol, 1-nonanol 및 1-decanil은 SPMV 외부 단층의 표면(친수성 부위)에 분포되는 것에 비하여 각각 949, 416.8, 214.8, 90.3, 53.7, 15.20, 6.80, 2.00, 1.03 및 2.40 배가 된다는 것을 확인하였다. 1-decanol은 $C_{10}$인데도 불구하고 $C_8$인 1-octanol에 비하여 적은 양이 소수성 부위에 침투 분포한다는 것이 확인되었다.

• 아시안잔디학회지
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• 제9권3호
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• pp.173-185
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• 1995

Hydroseeding technique is a very popular method of revegetating slope areas through the control of soil erosion and stability by seeding grasses. This study was conducted to select turfgrass species and cultivars for hydroseeding. Experiment plots were established on various soil types and environmental conditions at Singar-Ansan high-way construction site. The investigation was designed in three cutting, one back-filling and other three spare sites with various seed mixtures. Results indicated that combinations of seed mixtures influenced seed germination and rates of surface cover. In a view of long term, vegetation shifts should be influenced by characters of slopes and micro-climate conditions. Hydroseeding did not show good results on rocky slope areas. Revegetation was only going on where there had soil. The combination of seed mixture with a higher rate of perennial ryegrass had relatively good revegetation with faster germination and seedling growth. Improved turf-type tall fescue Arid ⓡ and Falcon ⓡ seemed to have good environ-mental adaptation and drought tolerance. Wild or old type cultivars showed relatively slow green-up in spring and growth rates at the next year of seeding. For the harmonious landscaping with surrounding area, the combination of native grass mixture with cool-season grasses had good results. Slow and low revegetation rate at hack-filling site seemed to be caused by the poor development of capillary tubes in sub-soil. It was shown that a high correlation between seed germination and revegetation rate, and between three-month later coverage rate and final rate. The evaluation of coverage rate after three month seems to he acceptable to decide the accomplishment of hydroseeding results on rode side slopes.

• 한국환경복원기술학회지
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• 제15권2호
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• pp.19-29
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• 2012

Numerous studies suggested that fish assemblage structure reflects the status of stream ecosystems. The status of streams integrity, including various trophic levels, water quality and habitat degradation, can be assessed by fish assemblages. In this study, we investigated the relationships between fish assemblages and streamline geometry of streams. Previous studies suggested that geomorphologic parameter can be a critical factor of permeability between adjacent two systems. From a landscape ecological perspective, edges may partially control the flow rate of energy between two adjacent systems. Thus, the Streamline geometry can be a geomorphologic parameter that exhibits the integrity of stream. We selected the Nakdong river for study areas, which is one of major rivers and the longest (525 km) River in South Korea. We used the revised IBI representing overall ecological characteristics of Korean fish assemblages and eight sub-assessment criteria of IBI, collected from 82 sampling sites in the Nakdong River. For calculating the Streamline geometry, we measured fractal dimension index that generally used in biology, ecology and landscape ecology. We used the digital land-use/land-cover map and generated a 1-km buffer for each sampling site and refined the shape of the Streamlines. Pearson correlation analyses were performed between Streamline geometry and IBI and sub-assessment criteria of IBI. The results show that IBI and eight sub-assessments of fish are significantly correlated with geometry of Streamline. The fractal dimension of Streamline geometry were related with IBI (r = 0.48) and six sub-assessments of IBI, including total number of native fish and native species, the number of riffle benthic species, sensitive species, tolerant species and native insectivore. Especially, the number of tolerant species(r = -0.52) and native insectivore(r = 0.52) show strong correlation with geometry of Streamline. These results indicate that lower Streamline geometry can result in poor fish assemblages, while higher geometry of Streamline can enhance fish assemblages by potentially supplying insects and better habitat conditions. We expect the results of our study to be useful for stream restoration and management. However, we see the necessity of study investigating the mechanisms how Streamline geometry affect fish assemblages.