• 한국조경학회지
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• 제34권4호
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• pp.48-60
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• 2006

This study was carried out to create a master plan for an urban eco-forest, including concepts of forest restoration, forest management, and nature education. The concept of an urban eco-forest is different from the original eco-forest, which pursues the conservation of native species in their habitats, using the resources of special plants, etc. The study site was a hill (128m) managed as an urban natural park. Citizens have usually used the park for fitness purposes, as a place for outdoor exercise early mornings on weekdays and holidays. The result of a questionnaire survey showed that the users knew about the eco-forest, and they wanted to participate in the nature education programs. The flora and actual vegetation were weak about conservation of native species on the site. The framework of the plan consisted of development of the ecological forest and forest restoration, and allowed for recreation and the experience of nature, according to the concepts of the urban eco-forest. The landscape was divided into the experience zone of biodiversity, the experience zone of a valley ecosystem, the forest restoration zone, and the recreation and experience zone in nature. Based on this framework, detailed programs and theme areas were planned, as were forest restoration models and planting plans.

• Journal of Microbiology and Biotechnology
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• 제13권1호
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• pp.134-138
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• 2003

The secondary and tertiary structures of ${\beta}$-galactosidase from L. lactis ssp. lactis 7962 were designed using Nnpredict and Sybyl version 6.3. By using site-directed mutagenesis, the mutated enzymes, Tyr-475-phe and Glu-506-Asp, were generated based on the structural modeling of L. lactis ssp. lactis 7962. The enzymes Tyr.-475-Phe and Glu-506-Asp had <$1\%$ of the activity of the native enzyme with ONPG as substrate. The $V_{max}$ values of the mutated enzymes were greatly reduced (1,800~40,000-1314) compared with the value for the native ${\beta}$-galactosidase. However, the $K_m$ values of Tyr-475-Phe and Glu-506-Asp with ONPG, PNPG, PNPF, and PNPA were not significantly different from those of the native enzyme. The results obtained support the suggestion that Tyr-475 and Glu-506 constitute very important parts of the catalytic machinery of the ${\beta}$-galactosidase.

• 한국조경학회지
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• 제21권1호
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• pp.139-148
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• 1993

The present experiments have been conducted to find out the plant's growth environments habitate, mode of life, characteristic of photosynthesis, habit of growth and propagation. The results of this study are as follows: 1. Sambucus williamsii var. Coreana distributes around all sides of native site without having any relation to altitude, inclination and direction. 2. In the native site, Robinia pseudo - acacia and its neighboring species were Prunus yedoensis, Acer pseudo-sieboldianum, Lindera obtusiloba and Staphylea bumalda. From 21 to 41 types of species were located in the vegetation of the quadrat area. 3. According to the variation of leaf temperature with the result of the change of net photosynthetic rate, the optimum temperature for growth is $25^{\circ}C$. 4. The rooted rate of vegetative propagation was the highest at 100ppm IBA plot and the lowest at 200ppm BA plot. 5. The rooted rates of Sambucus williamsii var. coreana in perlite 50% bed, vermiculite bed and peatmoss 50%+sand 50% bed are higher than others.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 2002년도 제9회 학술 발표회 프로그램과 논문초록
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• pp.30-30
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• 2002

The native forms of common globular proteins are in their most stable state but the native forms of plasma serpins (serine protease inhibitors) show high-energy state interactions. The high-energy state strain of a ${\alpha}$$_1$-antitrypsin, a prototype serpin, is distributed throughout the whole molecule, but the strain that regulates the function directly appears to be localized in the region where the reactive site loop is inserted during complex formation with a target protease.(omitted)

• Bulletin of the Korean Chemical Society
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• 제24권1호
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• pp.70-74
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• 2003

The heterogeneous bioluminescence immunoassay for digoxin was developed using photoprotein, native aequorin as a label and the site-directed immobilization technique based on avidin/biotin interaction. Aequorin is a bioluminescence protein, originally isolated from the jellyfish Aequoria Victoria and an attractive label in analytical applications because of sensitive detection due to virtually no background bioluminescent signal. Digoxin is a cardioactive drug, and its therapeutic level in serum is at low concentration with very narrow therapeutic index. The aequorin-digoxigenin conjugates were synthesized by the N-hydroxysuccinimide ester method and characterized in terms of bioluminescent residual activity. The resulting dose-response curve shows that the detection limit is $1.0\;{\times}\;10^{-10}\;M$ and a dynamic range is three orders of magnitude, which was obtained by $1.0\;{times}\;10^{-10}\;M$ conjugate and 0.9 μg/mL anti-digoxin antibody. Three structurally similar molecules to digoxin were examined for their cross-reactivity. None of these three compounds showed any crossreactivity with digoxin antibody employed in this study. Standard amounts of digoxin corresponding to the therapeutic range were spiked into the each serum solution. Study of the serum matrix effect indicated that correlation coefficient shows good agreement between luminescence light intensity between in buffer and in serum.

• 한국환경복원기술학회지
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• 제21권4호
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• pp.75-86
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• 2018

This research was thereby made to study and analysis ecological, landscape, and stability qualities of roadside cut Slopes in expressways and national highways that went through revegetation works. Also, evaluation items and framework for integrated assessment of plant structure and restored state for the maintenance were suggested. Following is the summary of the result. firstly, for stabilization of introduced native plant species on roadside cut slops, assessment on the post-project environmental management through long-term monitoring and maintenance works must be made. It signifies a form of authentic recovery and restoration by the native plants. second, current evaluation framework on the test beds is well managed with clear evaluation standards and table while its method of assessing the post management with only test bed results lacks conformity with this study site. Newly developed evaluation framework on the post construction sites is estimated to be used as assessment standards on any roadside construction sites. Third, after site investigation distinction was observed among different revegetating construction types in the long-term perspective. Due to the different duration time of vegetation state between seed-spray measures(degraded in time) and layer-spray measure(maintained), the use of coarse straw-mat mulching work or Coir net was suggested in long term maintenance of cut slopes. forth, segmentation of post environmental assessment is organized into three large categories of "ecological", "stabile", and "landscape" qualities regarding post restoration quality and stability of slope through native plants with which categories marks 50, 30, 20 points of rates. fifth, components of the post environmental assessment were segmented in twelve categories driving results from former experimental construction and newly focused items on site. In the future, it is possible to propose a customized assessment method considering the location and location of construction work if the research on the application of post-environment assessment methods for road construction surface recording is conducted systematically.

• 한국환경과학회지
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• 제15권12호
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• pp.1103-1107
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• 2006

Iris odesanensis Y.N. Lee is one kind of species among 54 endangered plant species designated by the Ministry of Environment in Korea. It is very rare native plant throughout the country. Therefore, this study was conducted to investigate the native Iris odesanensis in Mt. Naeyon for their distribution and ecological characteristics. They were mostly distributed mid-slope of the mountain from 443m to 498m altitude. They were located lower part of valley and provided enough humid condition. They had proper light and humidity conditions as they faced south south west, south south east, and south. They were also located at the places deciduous woody plants were distributed for proper light conditions for spring blooming. Iris flowered to use light at the period before the trees thickly covered with foliage. However this unfavorable light condition caused low percentage as 7.04% of blooming individuals in this study. In herbaceous, Vioia chaerophylloides(Regel) W. Hocker, Carex siderosticta Hance, Erythronium japonicum Decne., Oxalis obtriangulata Max., Hepatica asiatica Nakai, Lilium tsingtauense Gilg et cetera were appeared well. We propose that proper pruning of upper branches of the deciduous trees is needed for suitable light condition of management of native sites.

• 아시안잔디학회지
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• 제25권1호
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• pp.11-16
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• 2011

본 연구는 국내 해안 및 도서지역에 자생하고 있는 버뮤다그래스를 지역별로 수집하여 입지환경에 따른 형태적 변이 정도를 알아보고자 수행되었다. 이들 입지환경, 토양의 화학성과 형태적 특성을 조사하였다. 수집된 자생지에 따라 초장, 엽폭, 엽장, 잎털, 포복경 및 화수당 종자수 등에서 큰 변이를 보여 조사 형질 간에 다양한 변이를 보이는 것을 확인할 수 있었다. 광엽과 세엽 특성의 버뮤다그래스가 존재하고 있었으며, 광엽형의 버뮤다그래스의 경우 열악한 환경에서의 적응성이 매우 높아 중 저관리의 잔디면에 이용 가치가 높을 것으로 생각된다. 상기 입지 환경과 형태적 특성 조사 결과 및 수집된 유전자원들은 앞으로 유전자원으로서 보존 가치가 높으며, 유용유전자를 보존할 수 있는 체계가 마련되어야 할 것으로 판단된다.

• Journal of Ecology and Environment
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• 제35권3호
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• pp.235-242
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• 2012

Mikania micrantha, a neo-tropical vine, is spreading rapidly in the tropical part of Nepal and is now threatening the rural ecosystem including biodiversity and rural livelihoods. However, no attempt has been made to control the spread of M. micrantha. As a result, the vines are spreading freely and rapidly. After a thorough literature review and assessment of forest management practices, we proposed a manual cutting method, as it suits the Nepalese situation for several reasons: required labor is readily available, as local communities are managing forest patches, and the method does not have any potential adverse effects on non-target native species. Experimental plots were laid out in August 2011 to examine the effectiveness of manual cutting. Two different site types based on canopy coverage were selected and divided into three blocks based on cutting strategy. Four treatments were assigned across the experimental plots following a complete block design. We harvested above-ground biomass according to the assigned treatment. The results suggested that there should be at least two consecutive cuttings within a 3-week interval before flowering, and that three consecutive cuttings resulted in 91% mortality of the vines. In addition, cutting promoted regeneration of native plant species. Employing regular cutting operations can modify understory shade enhancing regeneration of native species, which is a desirable condition to constrain proliferation of M. micrantha. Periodic cuttings reduced the competitiveness of M. micrantha regardless of canopy openness, but native ground cover should be retained.

• 한국환경복원기술학회지
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• 제7권2호
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• pp.75-86
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• 2004

This study aims to figure out ecological characteristic of natural forests focusing on vegetation as a way of ecological renovation for the restoration of naturalness for golf courses that were constructed in the Country and to present vegetation models and appropriate tree species for the purpose. The study site is P golf club, which is located in Gapyong-gun, Kyeonggi Province. The site is within a forest where the grade from the natural ecology map is the first one and the level from the green index accounts for the eight, thus showing a typical environment for a golf course in terms of location. The location of the site explains a reason for restoration. The major substance of the study is to conduct ecological evaluation of vegetation structures around and inside the golf course and to present a vegetation model. In order to evaluate the ecological characteristics of the vegetation structures, the analysis of the study covered succession stages, multi-layer vegetation structures and species diversity. Plant communities that have high species diversity were selected for the vegetation model and proper density and species were proposed considering the number of species and individuals and distances between trees. The vegetation restoration model targets succession into an oak forest. Within a unit of 100 $m^2$, the recommended model include a tall-tree layer with 11 trees such as Quercus serrata and Quercus mongolica, a sub-tall-tree layer with 12 trees including Quercus mongolica, Quercus serrata, Prunus sargentii, Fraxinus rhynchophylla and Acer pseudo sieboldianum, a shrub layer with 32 trees from 16 species, and a grass layer with a cover rate of 45 %. The proposed vegetation restoration model needs to apply to : 1) damaged natural forests by the construction of golf courses; 2) boundaries between golf courses and surrounding forests; 3) buffer zones; 4) open spaces in between courses; and 5) areas between greens and tees where open spaces are available in a mass. In conclusion, one of the most important factors in presenting a vegetation model for the restoration of naturalness in the golf course and other damaged forests is to provide multi-layer vegetation structures that are composed of native species. As for the specific application for the site, it is recommended to manage the vegetation in such a way that the environment of the site can have a similar environment to the surrounding forest which is expected to succeed into an oaks-dominant one.