• 한국콘텐츠학회논문지
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• 제12권11호
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• pp.422-431
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• 2012

문화콘텐츠시대에서 다양한 소재 개발은 문화산업의 풍부한 콘텐츠를 제공하는데 매우 유용하다. 다양한 소재 가운데서 역사적, 문화적으로 검증된 소재를 조직화하고 특화하여 다른 분야와의 연계는 보다 많은 가능성을 생산하고 있다. 본 연구는 역사적 인물과 음식을 통하여 그 장소에 대한 아이덴티티를 강화하려는 목적을 갖고 있다. 이러한 방안으로 인물이 가지고 있는 역사적 사실에 기초한 장소성을 기반으로 음식 문화와 연계하여 그 범위와 성격을 보다 확장하는 유용한 방식에 대한 고찰이다. 장소적 범위는 이순신장군과 역사 문화적 관련성을 갖고 있는 장소를 중심으로 향토음식개발을 시도한 지역으로 대상을 한정하였다. 따라서 본 연구는 지역을 대표하는 역사적인 인물콘텐츠의 범주를 확대 생산하여 지역적 아이덴티티를 구축하고, 향토음식브랜드개발 및 활성화에 대한 효과적 방향 설정에 기여하고자 하였다.

• 한국식품과학회지
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• 제40권3호
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• pp.290-296
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• 2008

찹쌀 전분을 가교화 후 4가지 상업용 ${\alpha}$-amylase 효소와 반응시켜 CLE 찹쌀 전분을 제조하고 이들의 이화학적 특성을 연구하였다. CLE 찹쌀 전분의 팽윤력 및 용해도는 천연 찹쌀 전분에 비해 다소 증가되는 경향을 보였다. 등온흡습곡선에서는 CLE 처리에 따른 수분 감소현상을 보였으나 유의적인 차이는 없었다. RVA특성을 검토한 결과 Termamyl과 Liquozyme으로 처리한 찹쌀 전분은 전반적으로 효소에 의한 가수분해가 강하게 진행되어 온도에 따른 점도의 변화가 크게 나타나지 않았으며, Fungamyl과 Kleistase로 처리한 찹쌀 전분은 효소에 의한 가수분해가 상대적으로 미약하게 진행되어 가교화에 의한 특성을 더 많이 나타내었다. DSC 열적 특성의 경우 호화개시온도, 호화종결온도 그리고 호화온도범위, 호화 엔탈피 모두 각 전분간에 유의적인 차이가 나타나지 않았다. X-ray 회절 분석 결과 또한 CLE 찹쌀 전분과 천연 찹쌀 전분 모두 A형의 결정 형태를 나타내었고, 상대적 결정화도의 차이가 나타나지 않는 것으로 보아 가교화 및 가교화후 효소처리가 찹쌀 전분의 결정형영역에는 영향을 주지 않는 것으로 보여진다.

• 한국가금학회지
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• 제41권4호
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• pp.235-240
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• 2014

최근 국내 오리고기 산업이 점진적으로 증가하고 있으나, 국내에서 생산되고 있는 대부분의 오리 종자는 수입에 의존하고 있는 실정이다. 이러한 의존도를 낮추기 위해서 국립축산과학원에서는 토종오리 상용화 연구를 시작하였으며, 이에 토종오리 품종 및 개체 식별의 필요성이 대두되었다. 본 연구에서는 토종오리와 백색실용오리가 다른 야생오리 품종과 얼마나 연관되어 있는지를 알아보기 위해 미토콘드리아 DNA의 D-loop control 영역의 염기서열을 이용하여 계통분석을 수행하였다. 그 결과, 토종오리와 백색실용오리는 대부분의 야생오리 품종과는 구분이 잘 되지만, 청둥오리와는 분류가 되지 않는 것을 확인할 수 있었다. 또한, 토종오리와 백색실용오리 품종간의 분류 또한 이들 집단이 다수의 변이와 haplotype을 공유하고 있음을 확인할 수 있었다. 분석 결과, 11개의 염기 변이가 확인되었으며, 이 변이들은 8개 haplotype으로 구성되어 있었다. 이 중 토종오리에서만 확인된 haplotype 2를 제외한 3개의 haplotype(haplotype number 1, 3, 4)에서 토종오리와 백색실용오리가 동일한 haplotype을 가진 반면, haplotype 5, 6, 7, 8은 백색실용오리에서만 확인되었다. 이상의 결과를 바탕으로 D-loop control 영역은 토종오리와 야생오리의 품종 구분을 위한 기초 자료로 이용될 수 있을 것으로 판단된다.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2001년도 Proceedings of 2001 International Symposium
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• pp.149-153
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• 2001

To investigate the mode of bactericidal action for antimicrobial peptide, pediocin, synthetic and mutant pediocins were prepared by direct chemical synthesis. Native pediocin was purified from Pedio-coccus acidilactici M and its conformational structure and bactericidal functions were analyzed and compared to synthetic pediocin. Schematic mode of pediocin actions, how pediocin binds on the target cell membrane, penetrates and makes tunnel are proposed. For these purposes, primary and secondary structures of pediocin was analyzed and disulfide bond assignment was also done. The pediocin purified from P. acidilactici M had high effective bactericidal ability against gram positive bacteria, especially Listeria monocytogenes and was very stable at extreme pHs and even at high temperatures such as autoclaving temperature (121$^{\circ}C$). Pediocin was consisted of 44 amino acids with four cysteines. Novel synthetic peptides were achieved by solid phase peptide synthesis(SPPS) method. To explain the function of cysteine in C-terminal region, mutant pediocin, Ped[C24A＋C44A], was synthesized and their structural and biological functions were analyzed. Second mutant pediocin, Ped[KllE], was prepared to explain the function of lysine at 11 of N-terminal part of pediocin, especially loop of $\beta$-sheet, and to predict the initial binding site of pediocin. The native and synthetic pediocins was showed random coil conformation by spectropolarimetry in moderate conditions. This conformation was observed in extreme conditions such as high temperature and low and high pHs, also. Circular dichroism(CD) data also showed the existence of $\beta$-turn structure in N-terminal part both native and synthetic pediocins. A structural model for pediocin predicts that 18 amino acids in the N-terminal part of the peptide assume a three-strand $\beta$-sheet conformation. This random coil in C-terminal part of pediocin was converted to folding structure, helix structure, in nonpolar solvents such as alcohol and TFE. The disulfide bond between $^{9}$ Cys and $^{14}$ Cys was concrete and inevitable, however, evidences of disulfide bond between $^{24}$ Cys and $^{44}$ Cys was not. Data of Ped[C24A＋C44A], pediocin mutant showed that $^{44}$ Cys was required during killing the target cells but not inevitable, since Ped[C24A＋C44A] still have bactericidal activity but much less than native pediocin. Another pediocin mutant, Ped[KllE], had still bactericidal activity, was controversial to propose that positive charge like as $^{11}$ Lys in loop or hinge in bacteriocin bound or helped to binding to microorganism with electrostatic interaction between cell membrane especially teichoic acid and positive amino acid nonspecifically. The conformation of pediocin among native, synthetic and mutant pediocins did not show big difference. The conformations between oxidized and reduced pediocin were almost similar regardless of native or synthetic.

• 한국식생활문화학회지
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• 제25권3호
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• pp.270-284
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• 2010

The objective of this study was to familiarize people with the native local food of Chungnam Province by developing its regional food as a culture resource. Native Local Food Search and luxury itemization based on regionally-characterized stories enhances self-perception of the national culture, promotes appropriate local images to the public, and contributes to the local economy by increasing regional tourism. Therefore, this study researched local stories of cultural significance, that is, those connected to the history and originality of Chungnam Province, and developed contents related to Chungnam native local food. Features of the native local food were introduced by a story telling method in order to appeal to the five senses. The story was composed for easy understanding of the value of food, and the brand image of Chungnam was developed based on representative historical stories of the region. In this study, the following were developed as representative images of Chungnam: 'Royal meal table of King Moo-ryung' in Kongju was presented by recomposing the story of King Moo-ryung, a famouns king of the Baekje era; 'Mong-yoo-do-won's rice meal table' was introduced via Mongyoo-do-won's painting by Kyeon An, a famous painter of the Chosun era who was born in Seosan. The 'Royal meal table of King Moo-ryung' was set with food made from local farm products, demonstrating the flavor and elegance of the Baekje era. 'Mong-yoo-do-won's rice meal table' resembled Kyeon An's Mong-yoo-do-won-do, which has a background featuring early spring. The rice table was designed to remind people of a flamboyant painting like a scattered peach blossom leaf in the early spring. To verify the health effectiveness of each rice table, the function of each ingredient was investigated through 'Sik-ryo-chan-yo:a dietary treatment', which was published by Soon-Ui Cheon during the Chosan era. According to the results, most of ingredients are certainly beneficial to health, as was recognized in the early Choson era.

• 한국식품과학회지
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• 제39권5호
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• pp.534-540
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• 2007

유과 제조 시 제조 시간을 단축할 수 있는 소재를 개발하기 위하여 찹쌀전분을 $POCl_3$와 반응시켜 가교화 찹쌀전분을 제조하고 이들의 물리화학적 특성을 연구하였다. 가교화 찹쌀전분의 치환율은 0.04-2.73으로 나타났으며, 팽윤력은 일반 찹쌀전분에 비해 약간 증가되는 경향을 보였으며 용해도는 일반 찹쌀전분보다 낮은 경향을 보였다. 등온흡습곡선은 가교화에 따른 유의적인 차이를 보이지 않았다. 전분의 pasting 특성을 검토한 결과, 호화개시온도는 가교화에 따른 변화가 없는 것으로 나타났으며, 최대점도, holding strength, final viscosity, setback은 일반 찹쌀전분보다 높아지는 결과를 나타냈다. 열적특성은 호화개시온도, 최대호화온도, 호화종결온도 그리고 호화엔탈피 모두 각 전분간에 유의적인 차이가 나타나지 않았다. X-ray회절 분석 결과로 볼 때, 가교화 찹쌀전분 및 일반 찹쌀전분 모두 A형의 결정 형태를 나타내었고, 상대적 결정화도의 차이가 나타나지 않는 것으로 보아 가교화가 찹쌀전분의 결정형영역에는 영향을 주지 않는 것으로 보여진다. 가교화 찹쌀전분을 사용하여 제조한 유과의 경우 일반 찹쌀을 장시간 침지한 후 제조한 시료와 비슷한 팽화율을 나타내었으며 시료의 변색 반응이 나타나지 않는 것으로 보아 유과 제조용 소재로서 적합한 것으로 판단되었다.

• 한국조경학회지
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• 제22권2호
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• pp.133-157
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• 1994

This study was executed to find out the succession stage and the ecological niche of Lindera erythrocarpa Markino. Four sites were selected by field investigation. They are Jeondungsa and Jeongsusa of Kanghwa Island, Mt. Suri of Anyang and Mt. Gaya of Chungcheongnamdo. They located in the region which have the similar temperature with Seoul region or lower average temperature for winter than that of adjacent Seoul. In the four sites, L, erythrocarpa was appeard in canopy layer at L. erythrocarpa community in Jeondungsa, L. erythrocarpa-Q. serrata, Z. serrata-L. erythrocarpa community in Jeongsusa, Castanea crenata-L, erythrocarpa community, L. erythrocarpa-Q. serrata community in Mt. Gaya and in the rest of the sites, it lives in subtree and shrub layer. And in the four sites but Jeongsusa area, it correspond with Chang(1991)'s study that L. erythrocarpa is dominant species in the site impacted by human. L. erythrocarpa lives with Quercus spp. such as Q. serrata, Q. variabilis, Q. mongolica and Carpinus laxiflora but it's presumably a passing phenomena.

• Asian-Australasian Journal of Animal Sciences
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• 제29권3호
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• pp.315-320
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• 2016

Porcine beta-defensin-1 (PBD-1) gene plays an important role in the innate immunity of pigs. The peptide encoded by this gene is an antimicrobial peptide that has direct activity against a wide range of microbes. This peptide is involved in the co-creation of an antimicrobial barrier in the oral cavity of pigs. The objective of the present study was to detect polymorphisms, if any, in exon-1 and exon-2 regions of PBD-1 gene in Large White Yorkshire (LWY) and native Ankamali pigs of Kerala, India. Blood samples were collected from 100 pigs and genomic DNA was isolated using phenol chloroform method. The quantity of DNA was assessed in a spectrophotometer and quality by gel electrophoresis. Exon-1 and exon-2 regions of PBD-1 gene were amplified by polymerase chain reaction (PCR) and the products were subjected to single strand conformation polymorphism (SSCP) analysis. Subsequent silver staining of the polyacrylamide gels revealed three unique SSCP banding patterns in each of the two exons. The presence of single nucleotide polymorphisms (SNPs) was confirmed by nucleotide sequencing of the PCR products. A novel SNP was found in the 5'-UTR region of exon-1 and a SNP was detected in the mature peptide coding region of exon-2. In exon-1, the pooled population frequencies of GG, GT, and TT genotypes were 0.67, 0.30, and 0.03, respectively. GG genotype was predominant in both the breeds whereas TT genotype was not detected in LWY breed. Similarly, in exon-2, the pooled population frequencies of AA, AG, and GG genotypes were 0.50, 0.27, and 0.23, respectively. AA genotype was predominant in LWY pigs whereas GG genotype was predominant in native pigs. These results suggest that there exists a considerable genetic variation at PBD-1 locus and further association studies may help in development of a PCR based genotyping test to select pigs with better immunity.

• 한국조경학회지
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• 제28권6호
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• pp.1-15
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• 2001

The purpose of this study is to figure out the structural characteristics of urban plant community and suggest restoration model of Quercus mongolica in the case of Korean national Capital Region. The investigation areas were selected from urban area of Mt. Nam at Chung-Gu, suburban areas of Mt. Bong at Eunpyoung-GU, Mt. Sungju at Buchon City and non-urban areas of Mt. Suri at Kunpu City and Mt. Chonma t namyangju-City. After the main study field had been classified into the evaluation of the ecological characteristics and the modeling of the vegetation. We analyzed to evaluate the ecological characteristics of the forest structure -- successional stage, naturalness, multi-layer structure of the forest and species diversity, and the plant community structures. We have proposed vegetation restoration model based on the selection of proper plants, the number of individuals, diameter short area of breast height, the shortest distance between plants in non-urban area. As for successional stage, It was judged that the ecological succession may not be followed like the present stage of the surveyed areas in urban, suburban and non-ruban areas. As for the retention of naturalness and multi-layer structures of vegetation, In Quercus mongolica community, Robinia pseudo-acacia and Ailanthus altissima occurred in each layers at Mt. Nam, Mt. Bong and Mt. Sungju, and Eupatorium rugosum occurred in herbaceous layer at Mt. Nam. Consequently, the ecological restoration plan following the structure of the vegetation in Mt. Chonma seemed to be advisable in Q. mongolica community, there were less number of species and individuals in urban areas than those of non-urban areas. Planting of trees following the simulated native plant community of non-urban areas seemed to be required to promote the plants in urban areas. Considering the number of individuals up to three layers in each 400$m^2$ area, it was composed of twenty nine in canopy layer, forth nine in understory layer, 367 in shrub layer and 33.7% herbaceous ground cover in the Q.mongolica community. The suggested restoration model in this study is nan applicable model for the introduction in the cities, and this study shows that continuous experiments and field investigation on this model should be performed in the future.

• Journal of Microbiology and Biotechnology
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• 제12권2호
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• pp.242-248
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• 2002

Previously, it was reported that the nonhomologous C-terminal regions of the D-hydantoinases are nonessential for catalysis, but affect the oligomeric structure of the enzyme [3]. In an effort to further confirm the above observation, the C-terminal region-inserted enzyme was constructed by attaching a peptide (22 residues) at the C-terminal of the D-hydantoinase from Bacillus thermocatenulatus GH2, and its structural and biochemical properties were compared with both the wild-type and C-terminal region-truncated enzymes. As a result, native tetrameric D-hydantoinase was dimerized as the truncated enzyme, and the inserted mutant with a new sequence was expressed as a catalytically active form in E. coli. Expression level of the inserted and truncated enzymes were found to be significantly increased compared to the level of the wild-type enzyme, and this appears to be due to the reduced toxic effect of the mutant enzymes on host cells. Dimerized enzymes exhibited increased thermo- and pH stabilities considerably when compared with the corresponding wild-type enzyme. Comparison of the substrate specificity between the mutant and wild-type enzymes suggests that the substrate specificity of the D-hydantoinase is closely linked with the oligomeric structure.