• 한국임상수의학회지
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• 제7권1호
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• pp.415-418
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• 1990

To investigate the changes of adrenal gland associated with follicular cyst in Korean native cattle, adrenals ans ovaries were collected from 54 Korean native cattle at abbatoir. Adrenals were stained by H-E stain and they were examined under light microscope. Enlargement of adrenal cortex affecting fascicular and reticular zone appeared in cows associated with follicular cyst.

• 한국동물위생학회지
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• 제18권2호
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• pp.158-162
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• 1995

An abattoir survey was carried out on 300 Korean native cattles reared and slaughtered in North Kyeongnam area to determine the prevalence of Fasciola hepatica infections. The average prevalence of fascioliasis was 46.4% ; the infection rate had a tendency to increase with age. Some hematological and biochemical indices were assayed in Korean native cattle naturally harbouring Fasciola hepatica infection and compared with uninfection control. Affected animal revealed reduction total erythrocyte count and hemoglobin, which was consistent in total leucocyte count and packed cell volume. Infected cattle had lower level of serum total protein and albumin, was consistent serum calcium and glucose indices, had higher levels of SGOT, SGPT and cholesterol indices.

• 대한수의학회지
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• 제35권3호
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• pp.615-623
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• 1995

Sera from 304 Holsteins or Korean native cattle were collected from slaughterhouse in Kwangju area to study the infection of major virus-borne diseases. Serum antibody titers against infectious bovine rhinotracheitis virus(IBRV), bovine viral diarrhea virus(BVDV), parainfluenza type-3 virus(PI-3V), bovine ephemeral fever virus(BEFV), bovine Ibaraki virus(BIV), bovine Akabane virus(BAKV), bovine rotavirus(BRV), bovine coronavirus(BCV) were measured by serum neutralization tests. Results which obtained were as follows. Sera from 280 cattle(92.1%) contained antibodies against BRV which rate was the highest among the 8 viruses, and serum antibodies against BCV, BVDV, BIV, BAKV, BEFV, IBRV and PI-3V were detected from 266(87.5%), 149(49%), 108(35.5%), 94(30.9%), 80(26.3%), 32(10.5%) and 24(7.9%) cattle, respectively. Prevalence of seropositives to BVDV, BIV, BAKV, BEFV were higher among Holsteins than among the Korean native cattle(P<0.05). Prevalence of antibody titers against BVDV, BIV and BEFV in Korean native cattle were higher among females than males, while more males contained antibodies to BAKV, IBRV and PI-3V than females in their blood(P<0.05). Seropositives to BVDV, BIV, BAKV, BEFV and IBRV in Holsteins were higher among females than males(P<0.05). In Korean native cattle, serum antibody titers against IBRV and PI-3V ranged from 1:2~1:32 and 1:2~1:64, respectively, while serum antibody titers against the rest 6 viruses ranged from $1:2{\sim}1:{\geq}256$. In Holsteins, serum antibody titers against IBRV and PI-3V ranged from 1:2~1:64 and 1:2~1:32, respectively, while serum antibody titers against the rest 6 viruses ranged from $1:2{\sim}1:{\geq}256$.

• Parasites, Hosts and Diseases
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• 제39권3호
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• pp.227-232
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• 2001

Eight dogs were experimentally infected with Sarcocystis by oral inoculation of cardiac muscle from naturally infected cattle. The infected dogs commenced discharging of sporocysts in the feces after 10 to 12 days of inoculation, and continued until 20 and 35 days after inoculation. Three dogs were reinfected with cardiac muscle from the naturally infected cattle. Sporocysts reappeared in the feces on 12 to 13 days after reinfection. Sarcosystis sporocysts collected from the experimentally infected dogs were fed to each of the two 30-day-old Korean native calves. The infected calves remained clinically normal, except for the high fever (${\geq}{\;} 40^{\circ}C$) and decreased hematocrit values on day 30 to 40 post inoculation. Muscular cysts of Sarcocystis were found from infected calves on day 40 post inoculation. Proliferative forms of Sarcocystis were also observed in the muscle of infected calves. These results suggest that the Sarcocystis cruzi found in Korean native cattle has a 2-host life cycle with dogs as the definitive host and Korean native calves as the intermediate host.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
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• pp.51-51
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• 2003

Oct-4 is a maternally expressed octamer-binding protein encoded by the murine Oct-4 gene. It is present in unfertilized oocytes, but also in the inner cell mass and in primordial germ cells. In addition, Oct-4 is the first transcrition factor described that is specific for the blastocysts stage bovine embryos. The spatial and temporal expression patterns were further determined using Immunohistochemistry. With this technique Oct-4 protein expression is detected in the oocyte, in the blastocyst. After pregnancy Oct-4 expression is restricted ovary and placental tissue. Therefore Oct-4 is a transcription factor that is specifically expressed in cells participating in the pregnancy of Korean native cattle. These result suggest that Oct-4 localization and expression may contribute to the defects in the developmental normal seen in Korean native cattle.

• Parasites, Hosts and Diseases
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• 제41권3호
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• pp.141-145
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• 2003

This study was performed to investigate the prevalence of theileriosis and to compare the prevalence of this disease in Korean native cattle reared under different environmental conditions, namely, in a grazing area and a non-grazing area by polymerase chain reaction. Three hundred and one Korean native cattle (276 cows and 25 bulls) that had not received prior treatment or been vaccinated to prevent theileriosis were examined by PCR for Theileria sergenti infection from 2001 to 2002. In our study, the parasitemia range in T. sergenti-positive cattle by microscopy were from 0.1 to 3％ (mean 0.8％). In terms of mean prevalence, 204 of the 301 Korean native cattle (67.8％) were positive reaction by PCR. Our results also revealed that the infection rate among cows (70.3％) was significantly higher than that among bulls (40.0％) (p < 0.01). T. sergenti infection among the over 3 year-old-group (75％) had a significant higher prevalence than that among the less than 3 year-old-group (61.8％) (p < 0.05). Our data also showed that grazing areas (76.1％) had the significant higher prevalence than non-grazing areas (51％) (p < 0.001). In conclusion, this study demonstrates that the prevalence of T. sergenti infection is high and that its prevalence in grazing cattle is higher than that in non-grazing cattle, Therefore, life-long treatment and the development of an optimal vaccine are needed to reduce the numbers of bovine theileriosis in both grazing and non-grazing areas.

• Molecules and Cells
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• 제20권3호
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• pp.325-330
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• 2005

Ancient cattle bones were excavated from archaeological sites in Jeju, Korea. We used molecular genetic techniques to identify the species and establish its relationship to extant cattle breeds. Ancient DNA was extracted from four sources: a humerus (Gonae site, A.D. 700-800), two fragments of radius, and a tooth (Kwakji site, A.D. 0-900). The mitochondrial DNA (mtDNA) D-loop regions were cloned, sequenced, and compared with previously reported sequences of various cattle breeds (9 Asian, 8 European, and 3 African). The results revealed that these bones were of the breed, Bos taurus, and a phylogenetic tree indicated that the four cattle bones formed a monophyletic group with Jeju native black cattle. However, the patterns of sequence variation and reports from archaeological sites suggest that a few wild cattle, with a different maternal lineage, may have existed on Jeju Island. Our results will contribute to further studies of the origin of Jeju native cattle and the possible existence of local wild cattle.

• 한국식품과학회지
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• 제26권5호
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• pp.500-506
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• 1994

한우육(1등급), 유우육, 교잡종우육 및 호주산 수입우육의 등심부위를 구입하여 각종의 쇠고기 품질을 비교, 평가하기 위하여 연도, 다즙성 및 풍미에 관련되는 성분을 분석함과 아울러 관능검사를 실시하였다. 풍미에 관련되는 화학적 성분들로서는 비단백태질소, 핵산물질, 지방산, 유리아미노산 등을 분석하였고 쇠고기의 연도 측정을 위해 Hydroxyproline, 보수력, 근내지방함량 분석 및 Instron을 이용한 연도측정을 하였다. 또한 쇠고기의 기호성 및 풍미특성을 비교, 평가하기 위해 주관적 평가방법인 삼점검사와 정량적 묘사분석을 수행하였다. 풍미에 관련되는 비단백태질소, 핵산물질, 지방산, 유리아미노산 등을 분석한 결과, 비단백태질소는 한우육이 가장 낮아 도살후 저장기간이 가장 짧은 것으로 판단되며, 핵산물질은 한우육이 가장 높았다. 유리아미노산 함량은 한우육이 가장 낮았으나 염기성 아미노산과 방향족 아미노산의 상대적 비율은 가장 높았던 반면, 황함유 아미노산의 함량은 수입육이 가장 높았다. TBA가(價)는 한우육이 가장 낮았고, 지방산의 구성을 볼 때 한우육의 불포화지방산 비율은 수입우육이나 유우육보다 높았으나 교잡종우육과는 비슷하였다. 연도는 Instron을 이용한 경도측정에서 수입우육과 교잡종우육이 한우육과 유우육보다 질긴 것으로 나타났으며, 콜라젠 함량은 유우육이 가장 높았고 한우육이 가장 낮았다. 다즙성을 부분적으로 반영하는 보수력은 한우육이 가장 높았고 아울러 pH도 가상 높았다. 근내지방함량도 한우육이 가장 높아 다즙성이 우수한 것으로 판단되었다. 관능검사 결과, triangle test에서는 한우육과 수입우육, 한우육과 교잡종우육, 수입우육과 교잡종우육간의 차이를 관능검사요원들이 구별하였다. 그러나 정량적 묘사분석 결과 다즙성을 제외하고는 냄새, 풍미, 연도에서 품종간 차이가 발견되지 않았다. 한우의 비휘발성 풍미성분은 다른 쇠고기 시료에 비해 높은 경향을 나타냈음에도 불구하고 주관적 기호성 평가에서는 유의차를 나타내지 못했다. 이것은 한국인들이 아직 쇠고기 품질 특성에 대한 객관적 평가기준을 인식하지 못하고 있어 여러가지 쇠고기간의 비교평가는 할 수 있지만, 개별 쇠고기에 대한 절대평가는 제대로 할 수 없다는 것을 보여준다.

• 대한수의학회지
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• 제51권1호
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• pp.59-61
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• 2011

The prevalence of Toxoplasma (T.) gondii was surveyed using a latex agglutination test (LAT) in native Korean cattle. A blood sample was collected from female 105 cattle in the Daejeon area of Korea. All cattle were asymptomatic and had not received any prophylactic treatment for T. gondii. Blood samples were collected via the caudal vein. The cattle ranged in age from 2~6 years (mean 3.7 years). LAT detected antibody to T. gondii in four of 105 (3.8%) cattle. However, the hazard analysis and critical control point protocol has been applied to cattle farms and beef traceability has been strengthen.

• 농업과학연구
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• 제27권1호
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• pp.33-38
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• 2000

본 연구는 한우와 Holstein종의 $\kappa$ -CN 유전자 발현조절부위, 즉 $\kappa$ -CN gene 5'-flanking region에 대한 특성을 구명하기 위하여 specific primer를 이용하여 PCR(polymerase chain reaction) 방법으로 분석하였다. PCR 증폭 결과, 349bp 크기의 증폭산물을 얻었으며 한우와 Holstein종의 $\kappa$ -CN 유전자의 발현조절부위의 염기서열 분석결과에 있어서는 -82bp에서 -431bp까지 분석되었으며, 종간의 염기서열 비교에서 -386bp에서는 Holstein종에서는 T 염기가 한우에서는 C 염기로 치환되었으며, -241bp와 -192bp에서는 Holstein종에서는 T 및 C 염가가 존재하였으나, 한우에서는 결실되었다. 또한, -183bp에서는 한우에서는 T 염기가 존재하였으나, Holstein종에서는 염기가 결실되어 있음을 알 수 있었다. 또한 한우와 Holstein 종간의 상동성 분석에 있어서는 한우 $\kappa$ -CN 유전자 발현조절부위의 염기서열이 Holstein종과 98.9% 의 상동성을 보였다.