• Fisheries and Aquatic Sciences
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• 제20권11호
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• pp.29.1-29.7
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• 2017

The objective of this study was to determine a simple and reliable ploidy identification protocol for the rainbow trout (RT), Oncorhynchus mykiss, in the field condition. To evaluate the ploidy level and compare different detection protocols, triploid RT and gynogenesis were induced by UV irradiation and/or heat shock. The hatching rate at day 30 was 85.2% and the survival rate at day 90 was 69.4% (fingerling). The sex ratio of female RT was 93.75% in the gynogenesis group, illustrating that the UV irradiation inactivated the sperm DNA. The hatching rate and survival rate were 82.0 and 74.7%, respectively, in the triploid-induced group. The triploid induction rate by heat shock procedure was 73.9%. Cytogenetic protocols for ploidy identification such as chromosome counting, erythrocyte nuclear size comparison, and analysis of nucleolar organizing regions (NORs) by silver staining were compared. Silver nitrate staining showed the greatest success rate (22/23 and 32/32 for the triploid-induced group and gynogenesis group, respectively), followed by erythrocyte nuclear size comparison (16/23 and 19/32 for the triploid-induced group and gynogenesis group, respectively) and, lastly, chromosome preparation (2/23 and 6/32 for the triploid-induced group and gynogenesis group, respectively) with the lowest success rate. Based on our findings, silver staining for RT ploidy identification is speculated to be highly applicable in a wide range of research conditions, due to its cost-effectiveness and simplicity compared to other numerous ploidy detection protocols.

• 한국산업정보학회논문지
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• 제19권2호
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• pp.1-13
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• 2014

In IEEE 802.11p/1609-based vehicular networks, vehicles are allowed to exchange safety and control messages only within time periods, called control channel (CCH) interval, which are scheduled periodically. Currently, the length of the CCH interval is set to the fixed value (i.e. 50ms). However, the fixed-length intervals cannot be effective for dynamically changing traffic load. Hence, some protocols have been recently proposed to support variable-length CCH intervals in order to improve channel utilization. In existing protocols, the CCH interval is subdivided into safety and non-safety intervals, and the length of each interval is dynamically adjusted to accommodate the estimated traffic load. However, they do not consider the presence of hidden nodes. Consequently, messages transmitted in each interval are likely to overlap with simultaneous transmissions (i.e. interference) from hidden nodes. Particularly, life-critical safety messages which are exchanged within the safety interval can be unreliably delivered due to such interference, which deteriorates QoS of safety applications such as cooperative collision warning. In this paper, we therefore propose a new interference-aware Dynamic Safety Interval (DSI) protocol. DSI calculates the number of vehicles sharing the channel with the consideration of hidden nodes. The safety interval is derived based on the measured number of vehicles. From simulation study using the ns-2, we verified that DSI outperforms the existing protocols in terms of various metrics such as broadcast delivery ration, collision probability and safety message delay.

• 한국과학교육학회지
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• 제14권1호
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• pp.85-102
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• 1994

The purpose of this study was to analyze students' physics problem solving processes and to find the patterns of their problem spaces when high school and university students solved the physics problems. A total of 51 students in a high school and in two universities participated in this study. Their thinking processes in solving 5 physics problems on electric circuit were recorded by using 'thinking aloud' method and were transferal into protocols. 'The protocols were analyzed by the coding system of problem solving process. One of the major theoretical contributions of the computer simulation approach to problem solving is the idea of problem space. Such a concept of problem space was applied to physics problems on electric circuit in this study, and students' protocols were analyzed by the basic problem spaces which were made up from the item analysis by the researcher. The results are as follows: 1) On the average 4.0 test items among 5 ones were solved successfully by all subjects, and all of the items were solved correctly by only 19 persons among all of them. 2) In regard to the general steps of problem solving process, there was little difference for each item between the good solvers and the poor ones. But according to the degree of difficulty of task there was a good deal of difference. For a complex problem all of 4 steps were used by most of students, but for a simple one only 3 steps except evaluating step were used by most of them. 3) It was found in this study that most of students used mainly the microscopic approach, that is, a method of applying Ohm's law on electric circuit simply and immediately, not using the properties of electric circuits. And also it was observed that most of students used the soloing tom below, that is, a solving path in which they were the first to calculate physical Quantities of circuit elements, before they caught hold of the meaning of the given problem regardless of the degree of difficulty.

• Imaging Science in Dentistry
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• 제40권4호
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• pp.165-169
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• 2010

Purpose : To survey the radiographic examination protocol for lateral cephalometric radiographic examinations and to measure their patient doses in Korea and to compare the dose according to the size of hospital, the type of image receptor system, and the installation duration. Materials and Methods : The radiographic examination protocols (kVp, mA, and exposure time) for lateral cephalometric radiography were surveyed with 61 cephalometric radiographic equipments and their patient dose-area product (DAP) measured with a DAP meter (DIAMENTOR M4-KDK, PTW, Freiburg, Germany) for 51 cephalometric radiographic equipments. The radiographic examination protocols and patient doses were compared according to the size of hospital (university dental hospital, dental hospital, and dental clinic), the type of image receptor system (film-based, DR and CR type) and the installation duration, respectively. SPSS 12.0.1 for Windows (SPSS Inc., Chicago, USA) was used for independent t-test and ANOVA test. Results : The average protocols were 77.0 kVp, 12.7 mA, 6.2 second for cephalometric radiography. The average patient dose (DAP) was $128.0mGy\;cm^2$ and 3rd quartile dose (DAP) $161.1mGy\;cm^2$ for cephalometric radiography for adult male. There was no statistically significant difference at average patient DAP according to the size of hospital, the type of image receptor system, and the installation duration, repectively. Conclusion : The average patient dose was $128.0mGy\;cm^2$ and the third quartile patient dose $161.1mGy\;cm^2$ for lateral cephalometric radiography for adult male in Korea.

• 한국패류학회지
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• 제22권2호
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• pp.167-173
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• 2006

The embryos of marine bivalves have been commonly used in bioassays for quality assessments of marine environments. Although several standard protocols for the developmental bioassay of bivalves have been proposed, only a few trials for application of these protocols in environmental assessments or for the development of a new protocol with Korean species have been conducted. As such, there is a strong need to establish standard bioassay protocols with bivalves commonly found in Korean waters. To determine the sensitivity of Mytilus galloprovincialis to establish a standard bioassay, their fertilized eggs were exposed to six metals (Ag, Cd, Cr, Cu, Ni, and Zn). The order of biological impact was Ag > Cu > Ni > Zn > Cr > Cd and their lowest observed effective concentration were 5, 16.4, 25.4, 142, 187 and 1,500${\mu}g/l$, respectively. The proportion of normal larvae appeared to decrease linearly with the logarithm of each toxicant concentration within the tested range. The average values of median effective concentrations $(EC_{50})$ from the triplicate experiments for Ag, Cd, Cr, Cu, Ni, and Zn were 6.8, 1,797, 786, 16.6, 68.1, and 139.2${\mu}g/l$, respectively. There was a more than 100-fold difference in $EC_{50}$ values of Cu and Cd. The value of $EC_{50}$ or median lethal concentration of Cu was within the range observed for other bivalve developmental bioassays. The overall sensitivity of M. galloprovincialis in the present developmental bioassay was also similar to that of other marine organisms commonly used in aquatic bioassays (e.g. sea urchins, oysters). Hence, the bioassay using the embryo of M. galloprovincialis is considered to be a useful tool to monitor and evaluate the quality of marine aquatic environments.

• 한국산학기술학회논문지
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• 제10권9호
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• pp.2492-2500
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• 2009

본 연구에서는 베트남전 참전 군인 중 고엽제 후유(의)증 환자와 한국전쟁 참전 상이군인을 대상으로 전반적인 심리적 특성을 파악하기 위해 로샤 검사를 실시하고 반응결과를 한국전 참전 재향 군인 집단과 비교분석하였다. 이를 위해 베트남 참전 군인 중 고엽제 후유의증 환자 20명과 한국전쟁 참전 재향군인 21명을 대상으로 개별적으로 로샤 검사를 실시했다. 각각의 변인들을 Exner 종합체계(2006)의 구조적 요약의 대표적 군집들을 중심으로 비교분석하였다. 즉 핵심영역, 반응결정인, 결정적 특수지표에서의 차이를 비교분석하였다. 그 결과 베트남 참전 집단은 6.25 참전집단에 비해 (1)인지적 경직성 (2) 문제해결에 있어서의 비효율성, (3)정서억압 (4)과잉경계 (5)불안 (6)우울 등의 가능성이 더 높은 것으로 나타내었다. 이러한 결과들은 베트남전쟁 참전 재향군인에서 과거 전쟁 경험에서의 정신적 외상과 관련된 후유증이 지속되고 있을 가능성을 보여주는 것이다. 마지막으로 본 연구의 제한점과 시사점에 대해 논의하였다.

• Journal of Hospice and Palliative Care
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• 제22권2호
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• pp.49-66
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• 2019

의료기술 발달 및 경제개발 수준 상승 등으로 호스피스 완화의료(hospice & palliative care) 서비스를 받는 말기 환자들이 점점 늘어나고 있다. 이와 더불어 최근 호스피스 완화의료 분야 임상전문가들은 환자나 가족 대상 호스피스 완화의료 서비스의 질을 향상시킬 목적으로 하는 연구에도 많은 관심을 보이고 있다. 한편 정부의 연구대상자 보호 정책이 강화되면서 IRB(생명윤리심의위원회)에서도 호스피스 완화의료 연구의 연구계획서를 심의할 때 환자의 취약성과 관련된 다양한 윤리적 사안들을 더 엄밀하게 점검하고 있다. 그러나 말기 환자들의 삶의 질 향상 및 유지를 위해서는 질적으로 보장된 호스피스 완화의료 서비스가 제공되어야 한다. 이를 위해서 윤리적으로 안전한 호스피스 완화의료 연구의 시도가 지지되어야 한다. 이에 필자는 호스피스 완화의료 연구의 윤리지침을 종설 논문 형식으로 제안했다. 이는 윤리적으로 안전하고 과학적으로 가치가 있는 연구계획서를 준비해야 하는 연구자 및 그 연구계획서를 심의하는 IRB가 적절하게 참조할 수 있도록 해주기 위함이다.

• Restorative Dentistry and Endodontics
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• 제47권2호
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• pp.15.1-15.18
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• 2022

This review aimed to characterize the effect of direct restorative material types and adhesive protocols on marginal adaptation and the bond strength of the interface between the material and the proximal dentin/cementum. An electronic search of 3 databases (the National Library of Medicine [MEDLINE/PubMed], Scopus, and ScienceDirect) was conducted. Studies were included if they evaluated marginal adaptation or bond strength tests for proximal restorations under the cementoenamel junction. Only 16 studies met the inclusion criteria and were included in this review. These studies presented a high degree of heterogeneity in terms of the materials used and the methodologies and evaluation criteria of each test; therefore, only a descriptive analysis could be conducted. The included studies were individually evaluated for the risk of bias following predetermined criteria. To summarize the results of the included studies, the type of restorative material affected the test results, whereas the use of different adhesive protocols had an insignificant effect on the results. It could be concluded that various categories of resin-based composites could be a suitable choice for clinicians to elevate proximal dentin/cementum margins, rather than the open sandwich technique with resin-modified glass ionomers. Despite challenges in bonding to proximal dentin/cementum margins, different adhesive protocols provided comparable outcomes.

• 한국패류학회지
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• 제21권1호
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• pp.25-31
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• 2005

The embryos of marine bivalves have been commonly used in bioassays for the quality assessment of marine environments. Although several standard protocols for developmental bioassay with bivalves have been already proposed, there have been few trials for applying these protocols in environmental assessment, or for developing new protocol with Korean species. So, there is a strong need to establish the standard bioassay protocols using bivalves commonly found in Korean waters. Prior to developing a new protocol, it is essential to know the optimum conditions for the reliable bioassay procedures. Here, we established the purpose of this study to determine the optimum bioassay conditions for successful development of a common mussel, Mytilus galloprovincialis. The conditions considered as critical for developmental bioassay, and determined in this study were; (1) temperature, (2) salinity, and (3) initial density of embryo. The optimal temperature for developmental bioassay of M. galloprovincialis was determined as $15^{\circ}C$. At this temperature, the required time for the embryo to become veliger larva was 48 hr. The acceptable range of salinity for the embryotoxicity test using M. galloprivincialis was from 30 to 35 psu, which was narrower than that of the natural habitat of adult populations. The optimum density of embryo at the beginning of bioassay was 100 embryos/ml. Over this density, the proportion of normally developed larvae decreased significantly. The results obtained in this study will serve as a basis for preparation of the standard bioassay protocol using embryo of M. galloprovincialis.

• Journal of Korean Dental Science
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• 제2권2호
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• pp.5-11
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• 2009

Objectives : This study investigated the hypothesis that the dentin bond strength of self-etching adhesives (SEAs) may be improved by applying a coat of hydrophobic, neutral adhesive resin in addition to SEA. Method and Materials : The bond strengths of two SEAs - Experimental SEA (EX) and Adper Prompt (AP) - were measured with three bonding protocols. The D/E resin of All-Bond 2 was applied as the hydrophobic, neutral adhesive. Clearfil SE Bond (SE, self-etching primer system) and All-Bond 2 (AB, total etching system) were used as references. The following protocols were used: (1) EX1 (EX 1 coat); (2) EX2 (EX 2 coats); (3) EX+ (EX 1 coat + D/E resin); (4) AP1 (AP 1 coat); (5) AP2 (AP 2 coats); (6) AP+ (AP 1 coat + D/E resin); (7) SE (SE primer + SE bond); (8) SE+ (SE primer + D/E resin); (9) AB (etching + AB primer + D/E resin). Filtek Z250 composite resin was built up and the microtensile bond strength (MTBS) values of the specimens were compared. The fractured surfaces were observed using SEM. Results : When SEA was used as self-etching primer and hydrophobic, neutral adhesive was applied as well, MTBS was significantly higher than that when either one coat or two coats of SEA only were used (p < 0.05). Conclusion : The hydrophobic, neutral adhesive improved the integrity of the bonded interface obtained with SEA.