• 제목/요약/키워드: national environment

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NCS 기반 토양환경분야 국가기술자격 실기시험 평가방법에 관한 연구 (A Study on the Evaluation Method of National Technical Qualification for Soil Environment Based on NCS)

  • 이정규;배재근
    • 한국지하수토양환경학회지:지하수토양환경
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    • 제22권5호
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    • pp.98-104
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    • 2017
  • Human Resources Development of Korea has been practicing performance evaluation for occupational qualification licences in environmental sectors that include water, air, and wastes management, but not soil. The purpose of the study was to improve overall quality of the national qualification test in the soil sector to better train applicants based on National Competency Standards (NCS), which is designed to emphasize site-specific conditions and knowledge. This study systematically analysed the current licence test in the soil sector with respect to qualification units, performance criteria, knowledge, techniques, and attitude as described in NCS. Furthermore, test evaluation was conducted to examine the validity of the revised licence test. The test results indicated the revised test method would bring positive outcomes to the related industry. Upon the reviews and amendments by the field experts, the result of this study could serve as a framework to the development of more robust and reliable licence test.

Lipoxygenase Inhibitory Activity of Korean Indigenous Mushroom Extracts and Isolation of an Active Compound from Phellinus baumii

  • Lee, Seung Woong;Song, Ja-Gyeong;Hwang, Byung Soon;Kim, Dae-Won;Lee, Yoon-Ju;Woo, E-Eum;Kim, Ji-Yul;Lee, In-Kyoung;Yun, Bong-Sik
    • Mycobiology
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    • 제42권2호
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    • pp.185-188
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    • 2014
  • We investigated a total of 335 samples of Korean native mushroom extracts as part of our lipoxygenase (LOX) inhibitor screening program. Among the mushroom-methanolic extracts we investigated, 35 exhibited an inhibitory activity greater than 30% against LOX at a concentration of 100 ${\mu}g/mL$. Especially, Collybia maculata, Tylopilus neofelleus, Strobilomyces confusus, Phellinus gilvus, P. linteus, P. baumii, and Inonotus mikadoi exhibited relatively potent LOX inhibitory activities of 73.3%, 51.6%, 52.4%, 66.7%, 59.5%, 100.0%, and 85.2%, respectively. Bioassay-guided fractionation led to the isolation of inoscavin A from the methanolic extract of P. baumii, which showed the most potent activity and was identified by spectroscopic methods. Specifically, inoscavin A exhibited potent LOX inhibitory activity with an $IC_{50}$ value of $6.8{\mu}M$.

Prediction of Rice Embryo Proteins using EST-Databases

  • Woo, Sun-Hee;Cho, Seung-Woo;Kim, Tae-Seon;Chung, Keun-Yook;Cho, Yong-Gu;Kim, Hong-Sig;Song, Beom-Heon;Lee, Chul-Won;Jong, Seung-Keun
    • 한국육종학회지
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    • 제40권1호
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    • pp.1-7
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    • 2008
  • An attempt was made to link rice embryo proteins to DNA sequences and to understand their functions. One hundred of the 700 spots detected on the embryo 2-DE gels were microsequenced. Of these, 28% of the embryo proteins were matched to DNA sequences with known functions, but 72% of the proteins were unknown in functions as previously reported (Woo et al. 2002). In addition, twenty-four protein spots with 100% of homology and nine with over 80% were matched to ESTs (expressed sequence tags) after expanding the amino acid sequences of the protein spots by Database searches using the available rice EST databases at the NCBI (http://www/ncbi.nlm.nih.gov/) and DDBJ (http://www.ddbj.nig.ac.jp/). The chromosomal location of some proteins were also obtained from the rice genetic map provided by Japanese Rice Genome Research Program (http://rgp.dna.affrc.go.jp). The DNA sequence databases including EST have been reported for rice (Oryza sativa L.) now provides whole or partial gene sequence, and recent advances in protein characterization allow the linking proteins to DNA sequences in the functional analysis. This work shows that proteome analysis could be a useful tool strategy to link sequence information and to functional genomics.

Isolation and Identification of Entomopathogenic Fungus from the Pine Wilt Disease Vector, Monochamus alternatus Hope(Coleoptera: Cerambycidae) in Korea

  • Shin, Tae-Young;Choi, Jae-Bang;Bae, Sung-Min;Cha, Ye-Rim;Oh, Jeong-Mi;Koo, Hyun-Na;Woo, Soo-Dong
    • International Journal of Industrial Entomology and Biomaterials
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    • 제18권2호
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    • pp.125-129
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    • 2009
  • Entomopathogenic fungi were isolated directly from a cadaver of adult Monochamus alternatus supporting fungal sporulation, using a semi-selective medium and then screened several fungal colonies. The pathogenicity of each fungus was tested using oak longicorn beetle, Moechotypa diphysis, as substitutive insect. As the result, only one of them showed high pathogenicity against M. diphysis, with up to 100% mortality within 21 days of inoculation. Selected fungus was named as MaW1 and identified by Beauveria bassiana using microscopic examination and DNA analysis. Pathogenicity was also evaluated to M. alternatus.

First Report of Myrothecium roridum Causing Leaf and Stem Rot Disease on Peperomia quadrangularis in Korea

  • Han, Kyung-Sook;Choi, Seung-Kook;Kim, Hyeong-Hwan;Lee, Sung-Chan;Park, Jong-Han;Cho, Myoung-Rae;Park, Mi-Jeong
    • Mycobiology
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    • 제42권2호
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    • pp.203-205
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    • 2014
  • In 2010, symptoms of leaf and stem rot were observed on potted plants (Peperomia quadrangularis) in a greenhouse in Yongin, Korea. The causative pathogen was identified as Myrothecium roridum based on morphological data, internal transcribed spacer sequence analysis, and pathogenicity test. To our knowledge, this is the first report of M. roridum causing leaf and stem rot disease on P. quadrangularis in Korea and elsewhere worldwide.

Production of Thaxtomin A by Korean Isolates of Streptomyces turgidiscabies and Their Involvement in Pathogenicity

  • Kim, Young-Sook;Cho, Jun-Mo;Park, Duck-Hwan;Lee, Heung-Goo;Kim, Jeom-Soon;Seo, Sang-Tae;Shin, Kwan-Yong;Hur, Jang-Hyun;Lim, Chun-Keun
    • The Plant Pathology Journal
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    • 제15권3호
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    • pp.168-171
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    • 1999
  • Crude extracts of pathogenic Streptomyces turgidiscabies isolates produced necrotic reaction on potato slices. Necrosis was first visible 24 hr after application and increased in severity over several days. However, necrosis was not observed when crude extracts of nonpathogenic strains were applied onto tuber slices. Presence of thaxtomin A from crude extracts of pathogenic S. turgidiscabies was identified by thin layer chromatography and high performance liquid chromatography. Nonpathgenic strains did not produce thaxtomin A in oatmeal broth. Inoculation of potato tuber slices with thaxtomin A partially purified from crude extract of a pathogenic S. turgidiscabies ST5 reproduce necrotic reaction suggesting that thaxtomin A is a pathogenicity determinant in S. turgidiscabies.

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Outbreak of Rice Blast Disease at Yeoju of Korea in 2020

  • Chung, Hyunjung;Jeong, Da Gyeong;Lee, Ji-Hyun;Kang, In Jeong;Shim, Hyeong-Kwon;An, Chi Jung;Kim, Joo Yeon;Yang, Jung-Wook
    • The Plant Pathology Journal
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    • 제38권1호
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    • pp.46-51
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    • 2022
  • Rice blast is the most destructive disease threatening stable rice production in rice-growing areas. Cultivation of disease-resistant rice cultivars is the most effective way to control rice blast disease. However, the rice blast resistance is easy to breakdown within years by blast fungus that continually changes to adapt to new cultivars. Therefore, it is important to continuously monitor the incidence of rice blast disease and race differentiation of rice blast fungus in fields. In 2020, a severe rice blast disease occurred nationwide in Korea. We evaluated the incidence of rice blast disease in Yeoju and compared the weather conditions at the periods of rice blast disease in 2019 and 2020. We investigated the races and avirulence genes of rice blast isolates in Yeoju to identify race diversity and genetic characteristics of the isolates. This study will provide empirical support for rice blast control and the breeding of blast-resistant rice cultivars.

낙동강 본류에 출현하는 담수 태형동물 Pectinatella magnifica (Leidy 1851)의 서식환경 연구 (Freshwater Habitats of Pectinatella magnifica (Leidy 1851) Living in South Korea)

  • 정현기;이경락;최병기;권헌각;박혜경;정강영;유재정
    • 환경생물
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    • 제33권3호
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    • pp.352-359
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    • 2015
  • 본 연구는 낙동강 본류에 서식하는 Pectinatella magnifica의 출현양상을 조사하기 위해 발생시기인 2014년 7월~11월 동안 기본 분포조사와 출현밀도가 높은 지역을 대상으로 집중조사를 실시하였다. 그 결과, 낙동강 본류 구간 내 Pectinatella magnifica의 부착기질은 인공적으로 형성된 기질에서 12.3%, 자연기질에서 87.7%로써 자연기질에서 매우 높게 확인되었으나 자연기질에 포함된 식물군락의 특정 종에 따른 선호하는 정도는 유의한 차이를 보이지 않았다. 반면 본 조사 결과에서는 고착대상으로 하는 기질의 분포 정도의 차이는 P. magnifica의 분포 차이에 영향을 미치고 있음을 보여주었다. 그러므로 태형동물이 선호하는 출현기질의 증가는 P. magnifica의 성장 및 분포에 영향을 미치는 요인 중 하나로써 작용될 것으로 사료된다.