• Title/Summary/Keyword: nano-pattern

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Phytoplankton in the Waters of the Ieodo Ocean Research Station Determined by Microscopy, Flow Cytometry, HPLC Pigment Data and Remote Sensing (현미경, Flow Cytometer, HPLC 색소자료 및 원격탐사를 이용한 이어도 관측기지 주변수의 식물플랑크톤 연구)

  • Noh, Jae-Hoon;Yoo, Sin-Jae;Lee, Jung-Ah;Kim, Hyun-Chul;Lee, Jae-Hak
    • Ocean and Polar Research
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    • v.27 no.4
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    • pp.397-417
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    • 2005
  • Phytoplankton community structure and distribution pattern in the surface water around the Ieodo Ocean Research Station were investigated during seven cruises carried out from July, 2003 to October, 2004. Samples were analyzed using various tools including a microscope, flow cytometer, and HPLC. Satellite images were used to analyze spatio-temporal phytoplankton biomass distribution. SeaWiFS chlorophyll a (chl a) images showed that spring blooms occurred in April-May near the Ieodo Station, and these waters were under the influence of Changjiang Dilute Water during July-October. Also, during the July-October period, HPLC pigments data showed increasing zeaxanthin concentrations, a marker pigment of cyanobacteria whereas increasing concentrations of various other pigments such as fucoxanthin, peridinin, prasinoxanthia alloxanthin, 19'-hexanoyloxyfucoxanthin and chlorophyll b were noted during spring blooms. Such pigment marker data were consistent with picoplankton data analyzed by flow cytometer and nano-microplankton analyzed by microscope. The pigment-CHEMTAX method was used to drive the phytoplankton group apportioned chi a. Diatoms, chlorophytes, dinoflagellates, and cryptophytes comprised 25.8, 20.7, 15.9, and 14.1%, respectively, of the total chl a in May. Average cyanobacteria concentrations in July-October contributed 25.4% of the total concentration. This was the highest percent contribution and was followed by chlorophytes, diatoms, and prymnesiophytes. This study discusses results from various methods, similarities and differences in the results among those methods, and the application range of the results from different analytical methods. Also, the study reveals a detailed phytolpankton community structure in the waters around the Ieodo Station, and suggests future monitoring considerations in relation to cell morphology, ecology and diversity factors according to taxonomic groups.

SiGe Nanostructure Fabrication Using Selective Epitaxial Growth and Self-Assembled Nanotemplates

  • Park, Sang-Joon;Lee, Heung-Soon;Hwang, In-Chan;Son, Jong-Yeog;Kim, Hyung-Jun
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2009.05a
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    • pp.24.2-24.2
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    • 2009
  • Nanostuctures such as nanodot and nanowire have been extensively studied as building blocks for nanoscale devices. However, the direct growth of the nanostuctures at the desired position is one of the most important requirements for realization of the practical devices with high integrity. Self-assembled nanotemplate is one of viable methods to produce highly-ordered nanostructures because it exhibits the highly ordered nanometer-sized pattern without resorting to lithography techniques. And selective epitaxial growth (SEG) can be a proper method for nanostructure fabrication because selective growth on the patterned openings obtained from nanotemplate can be a proper direction to achieve high level of control and reproducibility of nanostructucture fabrication. Especially, SiGe has led to the development of semiconductor devices in which the band structure is varied by the composition and strain distribution, and nanostructures of SiGe has represented new class of devices such nanowire metal-oxide-semiconductor field-effect transistors and photovoltaics. So, in this study, various shaped SiGe nanostructures were selectively grown on Si substrate through ultrahigh vacuum chemical vapor deposition (UHV-CVD) of SiGe on the hexagonally arranged Si openings obtained using nanotemplates. We adopted two types of nanotemplates in this study; anodic aluminum oxide (AAO) and diblock copolymer of PS-b-PMMA. Well ordered and various shaped nanostructure of SiGe, nanodots and nanowire, were fabricated on Si openings by combining SEG of SiGe to self-assembled nanotemplates. Nanostructure fabrication method adopted in this study will open up the easy way to produce the integrated nanoelectronic device arrays using the well ordered nano-building blocks obtained from the combination of SEG and self-assembled nanotemplates.

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Preparation and Characterization of Electrospun Nanofibers Containing Natural Antimicrobials (천연 향균물질 함유 나노섬유의 제조 및 특성분석)

  • Kim, Young-Jin;Kim, Sang-Nam;Kwon, Oh-Kyoung;Park, Mi-Ran;Kang, Inn-Kyu;Lee, Se-Geun
    • Polymer(Korea)
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    • v.33 no.4
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    • pp.307-312
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    • 2009
  • The fabrication of PHBV nanofibers containing various plant polyphenols by electrospinning has been examined. It has been found that the average diameters of fibers increased by the adding of polyphenols. The resulting fibers exhibited a uniform diameter ranging from 340 to 450 nm. As the concentration of polyphenols increased, the diameter of fibers increased due to the hydrogen bonding interaction between the ester groups of PHBV and hydroxyl groups of polyphenols. The interaction between PHBV and polyphenols, which forms a complex together in solution, was verifed by UV measurement. ATR-FTIR analysis confirmed the existence of the hydrogen bonding interaction. The semicrystalline structure of the PHBV nanofiber was observed from XRD pattern. The crystallinity of PHBV nanofibers was increased by the adding of polyphenols. PHBV nanofibers containing polyphenols showed superior antimicrobial activities.

Evaluation of Friction Properties between Geostrip/Sandpaper Interface (지오스트립/샌드페이퍼 계면에서의 마찰특성 평가)

  • Lim, Ji-Hye;Byun, Sung-Won;Jeon, Han-Yong
    • Journal of the Korean Geosynthetics Society
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    • v.5 no.4
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    • pp.27-33
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    • 2006
  • Frictional properties between geostrip and sand paper interface were estimated considering soil particle size also the friction coefficients and angles were determined with normal stress. Three kinds of geostrips of design strength 50, 70, 100 KN/m were used and 5 sandpapers of P100, P220, P320, P400, P600 were used also. Shear strength between geostrip and sand paper interface with design strength showed big difference and this is due to the uniform surface pattern of each geostrip when contact to sandpaper without regard to design strength. Shear strength of geostrip was increased with design strength and geostrips/P100 sandpaper interface showed the biggest value. Finally, all of geostrips showed the decrease phenomena of post-peak strength and this is due to the abrasion of geostrip surface by shear test.

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Investigating the Morphology and Kinetics of Three-Dimensional Neuronal Networks on Electro-Spun Microstructured Scaffolds

  • Kim, Dongyoon;Kim, Seong-Min;Kang, Donghee;Baek, Goeun;Yoon, Myung-Han
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.08a
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    • pp.277.2-277.2
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    • 2013
  • Petri dishes and glass slides have been widely used as general substrates for in vitro mammalian cell cultures due to their culture viability, optical transparency, experimental convenience, and relatively low cost. Despite the aforementioned benefit, however, the flat two-dimensional substrates exhibit limited capability in terms of realistically mimicking cellular polarization, intercellular interaction, and differentiation in the non-physiological culture environment. Here, we report a protocol of culturing embryonic rat hippocampal neurons on the electro-spun polymeric network and the results from examination of neuronal cell behavior and network formation on this culture platform. A combinatorial method of laser-scanning confocal fluorescence microscopy and live-cell imaging technique was employed to track axonal outgrowth and synaptic connectivity of the neuronal cells deposited on this model culture environment. The present microfiber-based scaffold supports the prolonged viability of three-dimensionally-formed neuronal networks and their microscopic geometric parameters (i.e., microfiber diameter) strongly influence the axonal outgrowth and synaptic connection pattern. These results implies that electro-spun fiber scaffolds with fine control over surface chemistry and nano/microscopic geometry may be used as an economic and general platform for three-dimensional mammalian culture systems, particularly, neuronal lineage and other network forming cell lines.

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Taguchi method-optimized roll nanoimprinted polarizer integration in high-brightness display

  • Lee, Dae-Young;Nam, Jung-Gun;Han, Kang-Soo;Yeo, Yun-Jong;Lee, Useung;Cho, Sang-Hwan;Ok, Jong G.
    • Advances in nano research
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    • v.13 no.2
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    • pp.199-206
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    • 2022
  • We present the high-brightness large-area 10.1" in-cell polarizer display panel integrated with a wire grid polarizer (WGP) and metal reflector, from the initial design to final system development in a commercially feasible level. We have modeled and developed the WGP architecture integrated with the metal reflector in a single in-cell layer, to achieve excellent polarization efficiency as well as brightness enhancement through the light recycling effect. After the optimization of key experimental parameters via Taguchi method, the roll nanoimprint lithography employing a flexible large-area tiled mold has been utilized to create the 90 nm-pitch polymer resist pattern with the 54.1 nm linewidth and 5.1 nm residual layer thickness. The 90 nm-pitch Al gratings with the 51.4 nm linewidth and 2150 Å height have been successfully fabricated after subsequent etch process, providing the in-cell WGPs with high optical performance in the entire visible light regime. Finally we have integrated the WGP in a commercial 10.1" display device and demonstrated its actual operation, exhibiting 1.24 times enhancement of brightness compared to a conventional film polarizer-based one, with the contrast ratio of 1,004:1. Polarization efficiency and transmittance of the developed WGPs in an in-cell polarizer panel achieve 99.995 % and 42.3 %, respectively.

A machine learning-based model for the estimation of the critical thermo-electrical responses of the sandwich structure with magneto-electro-elastic face sheet

  • Zhou, Xiao;Wang, Pinyi;Al-Dhaifallah, Mujahed;Rawa, Muhyaddin;Khadimallah, Mohamed Amine
    • Advances in nano research
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    • v.12 no.1
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    • pp.81-99
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    • 2022
  • The aim of current work is to evaluate thermo-electrical characteristics of graphene nanoplatelets Reinforced Composite (GNPRC) coupled with magneto-electro-elastic (MEE) face sheet. In this regard, a cylindrical smart nanocomposite made of GNPRC with an external MEE layer is considered. The bonding between the layers are assumed to be perfect. Because of the layer nature of the structure, the material characteristics of the whole structure is regarded as graded. Both mechanical and thermal boundary conditions are applied to this structure. The main objective of this work is to determine critical temperature and critical voltage as a function of thermal condition, support type, GNP weight fraction, and MEE thickness. The governing equation of the multilayer nanocomposites cylindrical shell is derived. The generalized differential quadrature method (GDQM) is employed to numerically solve the differential equations. This method is integrated with Deep Learning Network (DNN) with ADADELTA optimizer to determine the critical conditions of the current sandwich structure. This the first time that effects of several conditions including surrounding temperature, MEE layer thickness, and pattern of the layers of the GNPRC is investigated on two main parameters critical temperature and critical voltage of the nanostructure. Furthermore, Maxwell equation is derived for modeling of the MEE. The outcome reveals that MEE layer, temperature change, GNP weight function, and GNP distribution patterns GNP weight function have significant influence on the critical temperature and voltage of cylindrical shell made from GNP nanocomposites core with MEE face sheet on outer of the shell.

Permeability of the Lateral Air Flow through Unstructured Pillar-like Nanostructures (비정형 기둥 형상을 가진 나노구조에서의 가스 투과성 실험 연구)

  • Hyewon Kim;Hyewon Lim;Jeong Woo Park;Sangmin Lee;Hyungmo Kim
    • Tribology and Lubricants
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    • v.39 no.5
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    • pp.197-202
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    • 2023
  • Recently, research on experimental and analytical techniques utilizing microfluidic devices has been pursued. For example, lab-on-a-chip devices that integrate micro-devices onto a single chip for processing small sample quantities have gained significant attention. However, during sample preparation, unnecessary gases can be introduced into the internal channels, thus, impeding device flow and compromising specific function efficiency, including that of analysis and separation. Several methods have been proposed to mitigate this issue, however, many involve cumbersome procedures or suffer from complexities owing to intricate structures. Recently, some approaches have been introduced that utilize hydrophobic device structures to remove gases within channels. In such cases, the permeability of gases passing through the structure becomes a crucial performance factor. In this study, a method involving the deposition and sintering of diluted Ag-ink onto a silicon wafer surface is presented. This is followed by unstructured nano-pattern creation using a Metal Assisted Chemical Etching (MACE) process, which yields a nanostructured surface with unstructured pillar shapes. Subsequently, gas permeability in the spaces formed by these surface structures is investigated. This is achieved by experiments conducted to incorporate a pressure chamber and measure gas permeability. Trends are subsequently analyzed by comparing the results with existing theories. Finally, it can be confirmed that the significance of this study primarily lies in its capability to effectively evaluate gas permeability through unstructured pillar-like nanostructures, thus, providing quantitative values for the appropriate driving pressure and expected gas removal time in practical device operation.

Seasonal Phytoplankton Growth and Distribution Pattern by Environmental Factor Changes in Inner and Outer Bay of Ulsan, Korea (울산만 내측과 외측에서 계절적 환경요인의 변화에 의한 식물플랑크톤 성장 및 분포)

  • LEE, MIN-JI;KIM, DONGSEON;KIM, YOUNG OK;SOHN, MOONHO;MOON, CHANG-HO;BAEK, SEUNG HO
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.21 no.1
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    • pp.24-35
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    • 2016
  • To assess the relationship between environmental factors and seasonal phytoplankton community structure, we investigated abiotic and biotic factors in Ulsan Bay, Korea. We divided the bay into two areas based on geographical characteristics and compared the difference in each factor between inner and outer bay with t-test statistics. As a result, temperature in the outer bay was higher than that of the inner bay during winter (t = -5.833, p < 0.01) and autumn (p > 0.05). However, opposite trend was observed during spring (t = 4.247, p < 0.01) and summer (t = 2.876, p < 0.05). Salinity was significantly lower in the inner bay than in the outer bay in winter, spring, and summer (p < 0.01). However, the salinity was not significantly different between the inner and the outer bay in the autumn (p > 0.05). In particular, high nutrient concentration was observed in most stations during winter season due to vertical well mixing. The nutrient concentration was significantly higher in surface layers of inner bay after rainfall, particularly in the summer. The relative contribution (approximately 70%) of < $20{\mu}m$ (nano and pico) size phytoplankton was increased in all seasons with continuously low nutrients from the offshore water due to their adaption to low nutrient without other large competitors. Interestingly, high population of Eutreptiella gymnastica was kept in the inner bay during the spring and summer associated with high DIN (nitrate+nitrite, ammonium) after river discharge following rainfall, suggesting that DIN supply might have triggered the increase of Eutreptiella gymnastica population. In addition, high density of freshwater species Oscillatoria sp. and Microcystis sp. were found in several stations of the inner bay that were provided with large amounts of freshwater from the Tae-wha River. Diatom and cryptophyta species were found to be dominant species in the autumn and winter. Of these, centric diatom Chaetoceros genus was occupied in the outer bay in the autumn. Cryptophyta species known as opportunistic micro-algae were found to have high biomass without competitors in the inner bay. Our results demonstrated that Ulsan Bay was strongly affected by freshwater from Tae-wha River during the rainy season and by the surface warm water current from the offshore of the bay during dry season. These two external factors might play important roles in regulating the seasonal phytoplankton community structures.

Estimate of Characteristics and Manufacture of Blood Substitutes (혈액대용물질의 제조 및 특성 평가)

  • Kim, Gi-Beum;Park, Jai-Koan;Kim, Seong-Jong;Kim, Jong-Soo;You, Il-Soo;Kim, Min-Ho;Hong, Chul-Un;Kim, Jin-Shang;Kang, Hyung-Sub
    • Korean Chemical Engineering Research
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    • v.46 no.3
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    • pp.626-632
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    • 2008
  • The purpose of this study was to enhance gas exchange by producing hemosome, a hemoglobin microencapsulated with phospholipid of egg, and perfluorocarbone(PFC) emulsion solution. In the experiment, stable emulsion solution with 437 nm of mean particle size could be produced by Flusol-DA sonication, and shortening of emulsion time could be attained with higher stability as well. $0.8{\mu}m$ sized hemosome could be produced by microencapsulation of hemoglobin with phospholipid extracted from egg yolk. The pattern of oxygen saturation curve of hemosome was S shape, which is similar to that found in normal blood, and $P_{50}$ was measured to be 24 mmHg. The oxygen saturation in the mixed solution of hemosome and blood in 1:4(V/V%) ratio was similar to that of normal blood, and the same result was found in the mixed solution of PFC emulsion and blood in 1:4(V/V%) ratio.