• KSBB Journal
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• 제23권1호
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• pp.44-47
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• 2008

Streptomyces peucetius가 생산하는 anthracycline 계열의 doxorubicin은 치료목적으로 사용되는 중요한 항암제 중 하나이다. Doxorubicin은 rhodomycin D에서부터 몇 단계의 생합성 과정을 더 거쳐 생산되는데, 생물학적 활성을 갖기 위해서는 deoxy-sugar의 전이가 반드시 일어나야 한다. 본 논문에서는 이종균주인 Streptomyces venezuelae에 11개의 유전자를 형질 전환하여 TDP-L-daunosamine를 생산하고 이것을 ${\varepsilon}$-rhodomycinone에 전이하여 rhodomycin D를 생산하는 연구를 수행하였다. S. peucetius 유래의 7개 유전자 dnmU, T, J, V, Z, Q, S.를 당 합성 및 전이를 위해 plasmid 형태로 전이하였으며, S. venezuelae의 desIII, IV와 doxorubicin 내성 유전자인 drrA, B는 chromosomal DNA에 삽입하였다. Aglycone 기질인 ${\varepsilon}$-rhodomycinone을 확보하기 위하여 6L의 고체 배지에 S. peucetius를 배양하여 유기용매로 추출하고 preparative HPLC로 분리 정제하였다. 결과적으로 이종균주인 S. venezuelae에서 ${\varepsilon}$-rhodomycinone에 당 전이가 일어난 생산물을 확인함으로써 deoxy-sugar의 생합성 및 전이에 필요한 최소한의 유전적 정보를 확인할 수 있었다. 또한, 유사서열 단백질 모델링을 통하여, 최초로 당 전이 반응에 필수적인 도움효소 DnrQ의 구조를 예측하였다.

• 한국지반환경공학회 논문집
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• 제14권3호
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• pp.41-49
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• 2013

회전관입형 말뚝은 말뚝설치 시 주변 흙의 변위가 발생하는 변위 말뚝으로 큰 지지력 및 인발력을 발휘할 수 있다. 또한 비배토 공법으로 슬라임이 발생하지 않고 시공 중 소음, 진동이 작아 환경친화적인 특성이 있어 외국의 경우 적용사례가 증가하고 있다. 하지만 대부분의 말뚝을 직접적으로 항타하는 항타말뚝, 선굴착 후 기 제작된 말뚝을 지중에 매입하는 매입말뚝이 주로 적용되고 있으며 회전관입형 말뚝의 사용은 제한적으로 이루어지고 있다. 지금까지 실시된 대부분의 실내시험에서는 말뚝을 설치한 후 지반을 조성하여 지지거동을 평가함에 따라 회전관입형 말뚝의 회전관입과정으로 인한 지지거동의 규명은 미흡한 실정이다. 이에 본 연구에서는 풍화토를 대상으로 회전관입과정을 모사한 실내실험을 실시하여 풍화토에 대한 지지력 거동을 평가하고자 하였으며, 더불어 나선형 판의 직경, 나선형 판의 간격, 나선형판의 개수, 나선형 판의 제원 등을 변화시켜가며 지지 거동을 평가하였다. 연구결과를 통하여 나선형 강관말뚝의 지지력 평가 결과 및 주요 제원에 따른 지지력 변화와 기존이론과의 비교분석 결과를 제시하였다.

• 한국환경보건학회지
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• 제29권1호
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• pp.51-61
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• 2003

Pollution purification using titanium dioxide (TiO$_2$) photocatalyst has attracted a great deal of attention with increasing number of relent environmental problems. Currently, the application of TiO$_2$ photocatalyst has been focused on purification and treatment of waste water. However. the use of conventional TiO$_2$ powder photocatalyst results in disadvantage of stirring during the reaction and of separation after the reaction. And the usage of artificial UV lamp has made the cost of photocatalyst treatment system high. Consequently, we herein studied the pilot-scale design to aid in optimization of the energy-saving process for more through development and reactor design by solar light/UV lamp/ TiO$_2$system. In this study, we manufactured the TiO$_2$sol by sol-gel method. According to analysis by XRD, SEM and TEM, characterization of TiO$_2$ sol were nano-size (5-6 nm) and anatase type. Inorganic binder (SiO$_2$) was added to TiO$_2$ lot to be coated for support strongly, and support of ceramic bead was used to lower separation rate that of glass bead The influences were studied of various experimental parameters such as TiO$_2$ quantity, pH, flow rate. additives, pollutants concentration, climate condition and reflection plate by means of reaction time of the main chararteristics of the obtained materials. In water treatment system, variable realtor as solar light/ or UV lamp according to climate condition such as sunny and cloudy days treated the phenol and E-coli(Escherichia coli) effectively.

• 대한약침학회지
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• 제15권4호
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• pp.42-51
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• 2012

Objectives: This study used the basic principle of Oriental medicine, the sovereign, minister, assistant and courier principle (君臣佐使論) to investigate the effects of the component of ONGABO, which is composed of Ginseng Radix (Red Ginseng), Angelica Gigantis Radix, Schisandrae Fructus, Cuscuta Semen and Curcumae tuber on the viability of HepG2 cells. Methods: Single and mixed extracts of the component of ONGABO were prepared by lypohilizing powder of Red Ginseng (6-year root from Kanghwa), Angelica Gigantis Radix, Schisandrae Fructus, Cuscuta Semen, Curcumae Tuber (from Omniherb Co., Ltd., Korea) at the laboratory of herbal medicine in Woosuk University and were eluted after being macerated with 100% ethanol for three days. The cell viability of HepG2 was determined by using an absorptiometric analysis with PrestoBlue (Invitrogen) reagent after the plate had been incubated for 48 hours. All of the experiments were repeated three times to obtain the average value and standard deviation. The statistical analysis was done and the correlation factor was obtained by using Microsoft Office Excel 2007 and Origin 6.0 software. Results: Although Ginseng Radix (Red Ginseng) and Schisandrae Fructus did not enhance the viability of HepG2 cells, they were shown to provide protection of those cells. On the other hand, Angelica Gigantis Radix decreased the viability of HepG2 cells significantly, Cuscuta Semen and Curcumae Tuber had a small or no effect on the viability of HepG2 cells. Conclusions: In the sovereign, minister, assistant and courier principle (君臣佐使論), Ginseng Radix (Red Ginseng) corresponds to the sovereign component because it provides cell protection effects, Angelica Gigantis Radix corresponds to minister medicinal because it kills cells, Schisandrae Fructus corresponds to the assistant medicinal to help red ginseng having cell protect effects. Cuscuta Semen and Curcumae Tuber correspond to the courier medicinal having no effect in cell viability in HepG2. We hope this study provides motivation for advanced research on the sovereign, minister, assistant and courier principle.

• 한국주조공학회지
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• 제29권2호
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• pp.64-69
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• 2009

In the present study the effect of cooling rate during solidification on the microstructural characteristics of Al-xAg (x = 31, 33, 35 at.%) in-situ binary eutectic composites has been investigated. To provide a wide range of cooling rate three different casting techniques, i.e. conventional casting, injection casting, and melt spinning have been used. The observed microstructure is very much dependent on the cooling rate. The fcc ${\alpha}$-Al and hcp $Ag_2Al$ phases exhibits an orientation of (111)Al//(0001)$Ag_2Al$, [1-10]Al//[11- 20]$Ag_2Al$. The microstructure of the melt-spun samples contains Widmanstatten structure resulting from solid-state transformation and nano scale two-phase structure resulting from solid-state phase separation. The microstructure of injection-cast samples contains eutectic structure and solid state phase-separated structure. On the other hand, conventional-cast samples exhibit a microstructure consisted of plate-type eutectic structure.

• Advances in nano research
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• 제9권4호
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• pp.263-276
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• 2020

In this article, the acoustic responses of free vibrated natural fibre-reinforced polymer nanocomposite structure have been investigated first time with the help of commercial package (ANSYS) using the multiphysical modelling approach. The sound relevant data of the polymeric structure is obtained by varying weight fractions of the natural nanofibre within the composite. Firstly, the structural frequencies are obtained through a simulation model prepared in ANSYS and solved through the static structural analysis module. Further, the corresponding sound data within a certain range of frequencies are evaluated by modelling the medium through the boundary element steps with adequate coupling between structure and fluid via LMS Virtual Lab. The simulation model validity has been established by comparing the frequency and sound responses with published results. In addition, sets of experimentation are carried out for the eigenvalue and the sound pressure level for different weight fractions of natural fibre and compared with own simulation data. The experimental frequencies are obtained using own impact type vibration analyzer and recorded through LABVIEW support software. Similarly, the noise data due to the harmonically excited vibrating plate are recorded through the available Array microphone (40 PH and serial no: 190569). The numerical results and subsequent experimental comparison are indicating the comprehensiveness of the presently derived simulation model. Finally, the effects of structural design parameters (thickness ratio, aspect ratio and boundary conditions) on the acoustic behaviour of the natural-fibre reinforced nanocomposite are computed using the present multiphysical model and highlighted the inferences.

• Structural Engineering and Mechanics
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• 제64권3호
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• pp.361-379
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• 2017

In this article, the vibration behavior of double-bonded sandwich microplates with homogeneous core and nanocomposite facesheets reinforced by carbon nanotube and boron nitride nanotube under multi physical fields such as 2D magnetic and electric fields is investigated. Symmetric and un-symmetric distributions of nanotubes are considered for facesheets of sandwich microplates such as uniform distribution and various functionally graded distributions. The double-bonded sandwich microplates rest on visco-Pasternak foundation. Material properties of sandwich microplates are obtained by the extended rule of mixture. The sinusoidal shear deformation theory (SSDT) is employed to describe displacement fields of sandwich microplates. Also, the dimensionless natural frequency is obtained by classical plate theory (CPT) and compared with the obtained results by SSDT. It can be seen that the obtained dimensionless natural frequencies by CPT are higher than SSDT. In order to study the material length scale parameters, modified strain gradient theory at micro scale is utilized and then, the equations of motion are derived using Hamilton's principle. The effects of different parameters such as foundation parameters including Winkler, shear layer and damping coefficients, various distributions and volume fraction of nanotubes, core to facesheet thickness ratio, aspect and side ratios on the dimensionless natural frequencies are discussed in details. The results of present work can be used to optimum design and control of similar systems such as micro-electro-mechanical and nano-electro-mechanical devices.

• Food Science and Biotechnology
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• 제14권5호
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• pp.704-708
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• 2005

Poly-y-glutamate (${\gamma}$-PGA) has great potential as a biodegradable polymer in a broad range of industrial fields such as food, cosmetics, medicine and water treatment. In order to isolate ${\gamma}$-PGA producers that are suitable for specific industrial applications, 653 Bacillus-like strains were isolated from 439 varieties of three Korean fermented foods, Cheongkukjang, Doenjang, and Kochujang, which were collected from different regions across Korea. A very high level of ${\gamma}$-PGA production was demonstrated in 4.7%, 1.8%, and 3.0% of the Bacillus-like strains isolated from Cheongkukjang, Doenjang, and Kochujang samples, respectively, which produced a viscous substance to such extent that it overflowed to the lid of the plate on the glutamate-dependent ${\gamma}$-PGA production plates. On glutamate-independent ${\gamma}$-PGA production plates, 5.1%, 5.9%, and 6.1% of Bacillus-like strains isolated from Cheongkukjang, Doenjang, and Kochujang samples, respectively, showed high production. The maximum ${\gamma}$-PGA production yields were 32.5 g/L and 5 g/L, depending on the purification methods in the glutamate-dependent media, with the higher yield resulting from a simple precipitation of ${\gamma}$-PGA by either methanol or ethanol and dialysis. The viscous substance produced by each strain showed different morphological characteristics, suggesting that isolated ${\gamma}$-PGA producers could produce various types of ${\gamma}$-PGA.

• Journal of Welding and Joining
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• 제31권5호
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• pp.47-53
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• 2013

We have investigated the traits of clad metals in hot-rolled clad steel plates, including the sensitization and mechanical properties of STS 316 steel plate and carbon steel (A516), under various specific circumstances regarding post heat treatment, multilayered welds, and thick or repeated welds for repair. For evaluations, sectioned weldments and external surfaces were investigated to reveal the degree of sensitization by micro vickers hardness, tensile, and etching tests the results were compared with those of EPR tests. The clad steel plates were butt-welded using FCAW and SAW with the time of heat treatment as the variable, a that was conducted at $625^{\circ}C$, for 80, 160, 320, 640, and 1280 min. Then, the change in corrosion resistance was evaluated in these specimens. With carbon steel (A516), as the heat treatment time increased, the annealing effect caused the tensile strength to decrease. The micro-hardness gradually increased and decreased after 640 min. The elongation and contraction of the area also increased gradually. The oxalic acid etch test and EPR test on STS316 and the clad metal showed STEP structure and no sensitization. From the test results on multi-layered and repair welds, it could be concluded that there is no effect on the corrosion resistance of clad metals. The purpose of this study was to suggest some considerations for developing on-site techniques to evaluate the sensitization of stainless steels.

• 대한기계학회논문집A
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• 제39권11호
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• pp.1099-1103
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• 2015

자연계에 존재하는 다양한 생명체 중 몇몇은 벽이나 천장을 자유자재로 걸어다닌다. 이러한 생명체 중 대표적으로 Gecko는 발가락 표피에 수십억개의 주걱모양의 나노헤어를 가지고 있으며, 이러한 구조로 인하여 높은 접착력을 가진다. 본 연구에서는, 도마뱀 발바닥 섬모와 같은 미세 마이크로 구조물에 금속코팅을 하여 접착특성을 향상시키는 것을 제안한다. 도마뱀 모방 인공섬모 구조물을 DRIE공법으로 준비된 몰드를 이용하여 PDMS로 제조하였다. 그리고, 인공섬모의 금속 코팅은 플라즈마 스퍼터링을 사용하여 수행하였다. 접착력과 내구성은 실험을 유리에 반복 접착 실험으로 평가 하였다. 접착성 및 내구성 실험을 통하여 인공섬모 구조물에 금속코팅이 미치는 영향에 대해 관찰하였다.