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Effect of Microbial Flora and Inoculation of Probiotics on Fermenting Characteristics of Naked Barley Grain (Hordeum Vulgare L.) (쌀보리 종실 내 미생물 분포와 생균제 접종이 발효특성에 미치는 영향)

  • Ahn, Hye-Jin;Kim, Ki Hyun;Jo, Eun Seok;Kim, Jo Eun;Kim, Kwang-Sik;Kim, Young Hwa;Song, Tae Hwa;Park, Jong Ho;Kang, Hwan Ku;Jang, Sun Sik;Oh, Young Kyoon;Cheon, Dong Won;Seol, Kuk-Hwan
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.35 no.4
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    • pp.321-326
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    • 2015
  • This study was performed to analyze the resident microbial flora and the effects of probiotic inoculation on the fermentation characteristics of whole grain naked barley (Hordeum Vulgare L.) with the goal of evaluating the possibility of utilization as fermented feedstuff. Naked barley grains were harvested 35 days after heading, and the microbial flora was analyzed using MALDI-TOF mass spectrometer. After inoculation of commercial microbes to the naked barley grain (BT), the pH and number of bacteria, such as aerobic bacteria, lactic acid bacteria, yeast and E. coli, were measured and compared with the non-inoculated control (BC). A total of 122 colonies was isolated from the naked barley grain and the most popular bacteria species was Staphylococcus xylosus (n = 30, 24.59%). The pH value decreased more rapidly in BT than in BC, and was significantly lower after 7 days of fermentation at $4.33{\pm}0.02$ and $4.83{\pm}0.01$, respectively. The number of aerobic bacteria, lactic acid bacteria and yeast showed an increasing trend within the first 7 days of fermentation, however, their numbers decreased at 28 and 42 days of fermentation. The population of lactic acid bacteria in BT was higher than in BC, but there was no significant different at 7 days of fermentation, with respective levels of $9.24{\pm}0.20$ and $9.01{\pm}0.10logCFU/g$ (p>0.05). The initial number of E. coli was very high in the naked barley grain but subsequently decreased significantly. After 7 days of fermentation, E. coli was not detected in either BT or BC samples. From these results, it appears that the fermentation of naked barley grain proceeded adequately after 7 days, and that fermentation contributes to the safety of naked barley grain during storage.

Studies on the Properties of Barley and Naked Barley Starch I. On the size frequency distribution of starch granules, amylose contents and blue value of starch (보리전분(澱粉)의 특성(特性)에 관(關)한 연구(硏究) I. 보리전분(澱粉)의 입경분포(粒經分布), Amylose 함량(含量), Blue value에 대하여)

  • Kim, Yong-Hui;Kim, Hyoug-Soo
    • Korean Journal of Food Science and Technology
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    • v.6 no.1
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    • pp.30-35
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    • 1974
  • As studies on the starch properties of barley and naked barley, the granule size frequency distribution, amylose content and blue value of starches seperated from the 11 varieties of barley and 13 varieties of naked barley, which were grown paddies of Office of Rural Development, Iri, Korea in 1972, were determined. The results were as follows: 1. The type of the barley and naked barley starch granules was short oval and the ratio of minor axis to major axis was 1 to 1.2. Diameter of the starch granules was in the range of $4.0{\sim}6.0\;{\mu}$ to $32.0{\sim}37.0\;{\mu}$ is and medium granule of the naked barley was $10{\sim}22\;{\mu}$ on the contrary to $16{\sim}20\;{\mu}$ of the barley starch. Average size of the naked barley starch was larger than that of the barley and some differences were observed between varieties. 2, Amylose contents of the barley and the naked barley starches were in the range $27.5{\sim}30.5%$ and average of the barley was 29.4% a little higher than 28.2% of the naked barley and observed of some differences between varieties. 3. Blue values of the barley and the naked barley starches were in the range $0.47{\sim}0.54$, and average was 0.51.

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Comparison of the Nutritional and Functional Compounds in Naked Oats (Avena sativa L.) Cultivated in Different Regions (재배지역 차이에 따른 쌀귀리 영양성분 및 기능성 성분 비교)

  • Ji-Hye Song;Dea-Wook Kim;Hak-Young Oh;Jong-Tak Yun;Yong-In Kuk;Kwang-Yeol Yang
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.68 no.4
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    • pp.402-412
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    • 2023
  • To cope with climate change, we compared the quality of naked oats (Avena sativa L.) cultivated in different regions. Naked oats were collected from domestic farms in different cultivation regions grouped as G1 and G2 for 3 years (2020-2022). The appearance, quality, and nutritional and functional compounds in the samples were assessed. In terms of appearance quality, the brightness and yellowness of the samples from the G1 region were significantly lower than those of the samples from the G2 region in 2020; however, no differences were observed between cultivation regions in the other 2 years. The results of testing the vitality of naked oats seeds showed that the electrical conductivity value was significantly lower in the samples from the G1 region than in those from the G2 region only in 2022. Among the nutritional components, moisture content was higher in the G2 region than in the G1 region over all 3 years, and the crude protein content was significantly higher in the G2 region than in the G1 region over all years. Carbohydrate content was significantly higher in the G1 region than in the G2 region in all 3 years and was inversely proportional to the crude protein content. The crude fat content tended to be significantly higher in the G1 region than in the G2 region, except in 2022. The levels of beta-glucan, a functional compound rich in naked oats, ranged between 3.4% and 4.2%, and except in 2020, there was no significant difference between cultivation regions. In addition, the content of avenanthramides, representative functional compounds that exist only in oats, was assessed. Over 2 years, in 2021 and 2022, the avenanthramide content was in the range of 2.4-20.7 ㎍/g and tended to be significantly higher in the G2 region than in the G1 region in both years. According to a survey of the average and minimum temperatures during the growing season of naked oats from 2020 to 2022, the average and minimum temperatures in January in the G2 region, which is the cultivation-limit area, were similar to those in Haenam in the G1 region. In conclusion, differences in nutritional and functional compounds were observed in naked oats grown in different cultivation areas. Therefore, considering the cultivation area of naked oats is expanding because of climate change, changes in the compounds that affect quality should be investigated.

Immunocompetence Status of White Plumage Naked Neck versus Normally Feathered Broilers in Tropical Climate

  • Patra, B.N.;Bais, R.K.S.;Sharma, D.;Singh, B.P.;Prasad, R.B.;Bhushan, B.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.4
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    • pp.560-563
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    • 2004
  • The study was undertaken to evaluate the effect of naked neck gene on mortality, cell mediated and humoral immune response in white plumage broiler population. The mortality of homozygous naked neck (Na/Na) broilers (11.71%) was comparatively lower than that of heterozygous naked neck (Na/na) (12.28%) and normally feathered (na/na) (13.59%) broilers. The humoral immune response was measured against (1% v/v) sheep red blood cells (SRBC) for total haemagglutinin (HA) antibody, 2-mercaptoethanol resistance (MER) or (IgG) antibody and 2-mercaptoethanol sensitive (MES) or (IgM) antibody titre on 7 days post-immunization. The titre was expressed as log2 of the highest dilution which shows complete haemagglutination. Total HA titers of Na/Na and Na/na (11.05$\pm$0.53 and 11.09$\pm$0.38) were comparatively higher than that of na/na (10.26$\pm$0.42). The MES antibody titre of Na/Na (8.50$\pm$0.53) and Na/na (7.63$\pm$0.45) broilers were significantly higher as compared to na/na (6.11$\pm$0.32) broilers. The MER titre of na/na genetic group (4.15$\pm$0.42) was significantly higher than Na/Na (2.55$\pm$0.37) and comparatively higher than Na/na (3.45$\pm$0.38) broilers. In vivo cell response to phytohaemagglutinin-P (PHA-P), measured as Foot Index (FI) in mm expressed significantly higher response in Na/na (0.473$\pm$0.05) and Na/Na (0.413$\pm$0.04) broilers as compared to na/na (0.304$\pm$0.03) broilers. The result of present study suggested that white plumage naked neck broilers had better immune response as compared to normally feathered broilers.

Structural Properties of Naked and Covered Barley Starches (쌀보리와 겉보리 전분의 분자구조적 성질)

  • Choi, Jun-Bok;Lee, Shin-Young
    • Korean Journal of Food Science and Technology
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    • v.21 no.1
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    • pp.80-85
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    • 1989
  • The structural properties of covered and naked barley starches and those components were investigated. ${\beta}-amylosis\;limit(%)$ of covered and naked barley starches were 58.6 and 56.3%, respectively and those of their amyloses and amylopectins were 87.0, 77.7, 57.6 and 52.0%, respectively. The ratios of outer chain length ${\overline{OCL}}$ and inner chain length${\overline{ICL}}$ for amylopectins of covered and naked barley were about 2.2:1 and 2.0:1, respectively. The elution curves by Sephadex G-75 after debranching starches with pullulanase were similar patterns for two starches and yielded two peaks consisting of amylose component${\overline{dp}}>55$ and bimodal amylopectin components of ${\overline{dp}_(35-45)$ and ${\overline{dp}}(10-20)$ hydrolysates. Also, hydrolysis products by sequential hydrolysis with pullulanase and ${\beta}-amylase$ contained 0.03-0.5% non-hydrolyzed peak components of ${\overline{dp}}>55$. The elution profiles of ${\beta}-amylase$ hydrolysates yielded two peaks consisting of the inner components${\overline{dp}}>55$ and the outer chain components of amylopectin${\overline{dp}}>10$ regardless of samples. However, ${\overline{dp}}$ distributions of ${\overline{dp}>55}$ hydrolysates exhibited the significant differences due to the varieties.

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Safety evaluation of gene therapy - a case study of naked DNA product

  • Ahn, Byung-Ok
    • Proceedings of the Korean Society of Toxicology Conference
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    • 2003.10b
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    • pp.86-86
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    • 2003
  • Gene therapy is a medical intervention based on modification of the genetic material of living cells. Gene transfer usually conducted using bacterial plasmid DNA and/or virus vector to express a specific protein. Gene transfer medicinal products classified as naked nucleic acid, complexed nucleic acid or non-viral vectors, viral vector, and genetically modified cells according to biological origin.(omitted)

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Relationship of Physicochemical Characteristics and Ethanol Yield of Korean Barley (Hordeum vulgare L.) Cultivars

  • Lee, Mi-Ja;Kim, Yang-Kil;Park, Jong Chul;Kim, Young-Jin;Kim, Kyeong-Hoon;Choi, Induck;Choi, Jae-Seong;Kim, Kee-Jong;Kim, Hyung-Soon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.57 no.4
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    • pp.401-408
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    • 2012
  • The grain and agronomic characteristics of Korean barley cultivars were investigated with respect to ethanol yield. Test weight, grain yield, and starch yield showed noticeable variation among the cultivars. Grain yields were higher in covered barley and non-waxy barley. Starch yield was higher in non-waxy barley than waxy barley. Protein, ${\beta}$-glucan, and starch content of tested cultivars ranged in 10.0-12.9%, 4.4-7.5% and 49.7-65.3%, respectively. Naked barley cultivar had higher starch content than covered barley cultivar. However, covered barley had high starch yield because it has higher grain yield than naked barley. Covered barley cultivar had higher husk content, ranging 7.6-14.0%, than that of naked barley cultivar, ranging 5.3-8.0%. Starch content was positively correlated with amylose content, test weight, ethanol yield and negatively correlated with protein, husk, ${\beta}$-glucan content. Ethanol yield per ton was positively correlated with starch content, but negatively correlated with husk content. Ethanol yield per hectare was positively correlated with starch yield, grain yield, grain weight and negatively correlated with protein, test weight. From this research, the important characteristics of barley cultivar as a bioethanol producing material were starch content and grain yield. Optimum barley genotype was non-waxy naked barley that had low protein, ${\beta}$-glucan, husk content, and high starch content and grain yield.

Hydration Properties of Naked Barley by Kernel Sizes (쌀보리의 입자별 수분 흡수 특성)

  • Yun, Young-Jin;Kim, Kwan;Kim, Sung-Kon;Kim, Dong-Youn;Park, Yang-Kyun
    • Applied Biological Chemistry
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    • v.31 no.1
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    • pp.13-20
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    • 1988
  • The hydration characteristics of four naked baley varieties by different kernel sizes were investigated. The predominant kernel size was 7 mesh, followed by 10 mesh kernel, of which comprised $86{\sim}94%$ of the total kernel. Diffusion coefficients of naked barleys at $40^{\circ}C$ increased as the kernel sizes decreased. The volume increases of naked barleys were linearly related to the moisture gain, regardless variety and kernel size.

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Drying Characteristics of Fluidized Bed Drying of Naked Barley (쌀보리의 유동층 건조 특성에 관한 연구)

  • Kim, Hee-Yun;Cho, Duk-Jae;Chung, Gea-Hwan;Hur, Jong-Wha
    • Korean Journal of Food Science and Technology
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    • v.25 no.5
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    • pp.558-564
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    • 1993
  • The drying characteristics of fluidized bed drying with different drying conditions using naked barley were carried out. This fluidized drying mechanism of naked barley was consisted of consecutive two falling rate parts, first falling rate period and second falling rate period without showing constant rate period. The drying rate constant was increased with decreasing charged amount and relative humidity and increasing air temperature and air velocity. Since the drying rate constant expressed by Arrhenius type equation in the falling rate period showed good linearity, the falling rate period was considered as the controlling step. The activation energy of first falling step was 1,900 cal/gmol, while for second falling step the values showed 2,500 cal/gmol.

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Preparation and Evaluation of Dried Noodles Using Barley-Wheat and Barley-Soybean Flours (보리-밀 및 보리-콩 복합분(複合粉)의 제면성(製麵性) 및 제품특성(製品特性)에 관(關)한 연구(硏究))

  • Cheigh, Hong-Sik;Ryu, Chung-Hee;Kwon, Tai-Wan
    • Korean Journal of Food Science and Technology
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    • v.8 no.4
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    • pp.236-241
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    • 1976
  • A barley (20-30%)-wheat (80-70%) flour gave generally acceptable noodle-making characteristics, in which naked barley flour showed somewhat better results than covered barley flour. And also for over 40% barley flour in the barley-wheat flour, the addition of xanthan gum improved the noodle-making characteristics. A naked barley-defatted soy flour could make dried noodles with only high NSI (Nitrogen Solubility Index) defatted soy flour, however this mixture was not considered to be feasible for noodle-making. In cooking characteristics of barley-wheat flour noodles, naked barley flour was more acceptable than covered barley flour and xanthan gum influenced the water absorption and volume expansion of noodles during cooking. The firmness, cohesiveness and gumminess in cooked noodles made of the barley-wheat flour increased and then decreased as the amounts of barley increased. The mixing of defatted soy flour (high NSI) to naked barley flour increased the firmness and gumminess of cooked noodles.

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