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Properties of Alcohol Fermentation from Barley Treated with Non-steam and Steam (비열 및 열처리에 따른 보리의 알코올발효 특성)

  • Woo, Seung-Mi;Kim, Tae-Young;Yeo, Soo-Hwan;Kim, Sang-Bum;Jeong, Yong-Jin
    • Food Science and Preservation
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    • v.14 no.2
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    • pp.201-206
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    • 2007
  • This study was designed to investigate quality characteristics of alcohol fermentation from 5 barldy cultivars teated with non-steam and steam. In alcohol fermentation treated with non-steam, alcohol concentration reached to 1.7% for naked berley, 2.9% for naked waxy barley and 4.3% for Hinchalssalbori. However, no fermentation was occurred with baney and waxy barley. When the barley cultivars were steamed, alcohol concentrations showed to be higher than those not steamed. In sugar concentration from non-steam, both barley and waxy barley showed to have approximately $9.0\;^{\circ}Brix$ and naked barley, naked waxy barley and Hinchalssalbori showed to have approximately $5.0\;^{\circ}Brix$. When the burley cultivars were steamed, sugar concentration was decreased as alcohol concentration was increased Interestingly, sugar concentrations in barley cultivars were fast dropped more than 50% following alcohol fermentation except naked barley k alcohol fermentation treated with non-steam, total acidity of Hinchalssalbori was 0.51%, but the others showed more than 0.80%. So except for Hinchalssalbori, it was possible that acidification occurred during the fermentation. In total acidity from steam, all samples showed less than 0.3%. In flee sugar from non-steam, total free sugar contents of barley and waxy barley were approximately 4,000.0mg% and contents of naked barley naked waxy barley and Hinchalssalbori were each approximately 300.0 mg%. Free sugar from steam was increased as sugar concentration increased. In conclusion, barley cultivars were evaluated to be not suitable at alcohol fermentation treated with non-steam.

Studies on the Effective Drug Delivery System Using Naked Plasmid DNA for the Erythropoietin Expression in vivo

  • Park, Yeong-Seop;Jeong, Dong-Geon;An, Jin-Ho;Choe, Cha-Yong;Ju, Hyeon
    • 한국생물공학회:학술대회논문집
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    • 2003.04a
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    • pp.582-586
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    • 2003
  • There has been interest in developing gene therapy based on naked plasmid DNA for treating serum protein deficiencies and human erythropoietin (hEPO) is one of the candidate for gene therapy being Investigated most enthusiastically. We constructed novel plasmid DNA vectors pVAC-hEPOI/II/III which contain one, two, three hEPO gene(s) respectively for producing high level expression and secretion of hEPO in vitro and in vivo. NIH3T3 and COS7 cells were transfected transiently with these vectors and increase in hEPO expression in medium reached 2-5 fold in comparison with pSecTagB-hEPO. Intra muscular administrations of pVAC-hEPOI/II/III vectors into mice resulted in high level secretion of hEPO in the serum and corresponding increases in hematocrit level. In conclusion the transduction efficiency of naked plasmid vectors is one of the critical factors of a gene delivery system and these novel plasmid vectors will contribute to various gene therapy based on naked plasmid DNA.

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A study on Enhanced Efficiencies of Methane Fermented Alcohol Wastewater Treatment by Supplement of Nutrients (영양물질 주입에 의한 메탄 발효 주정폐액의 효율증진에 관한 연구)

  • 안승구;이인학;진서형
    • Journal of Environmental Health Sciences
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    • v.23 no.3
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    • pp.40-49
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    • 1997
  • In Korea, naked barley and tapioca are main raw materials for the production of fermentation ethyl alcohol, and one million drums bf 95% fermentation ethyl alcohol is produced per year by use of them. Stillage of alcoholic fermentation is mostly digested by methane fermentation process, and methane gas occured if methane fermentation process is recovered and mixed with fuel to decrease 25-30% for total fuel used in factories. In the anaerobic digestion process of naked barley stillage, supplement of nutrients is necessary to slove the problems caused by inhibitory materials contained if stillage and deficiency of nutrients. Therefore, the objective of this study was to examine why the anaerobic digesters using the naked barley distillery wastewater have shown the poor digestability frequently and how to control it. As the poor digestion was supposed to be occurred by the lack of iron as trace nutrient, the experiments were carried out to find out the optimum dosage and the way of addition of iron and to assess the quantitative evaluation of the type of iron in digesters. Initially, bottle test as batch digesters and lab-scaled continuous flow digesters were used in order to determine the digestion characteristics with tapioca and naked barley distillery wastewater. According to the results of batch tests, the poor digestion was caused by volatile fatty acids and could be improved by adding of calcium. The activity of the methanogenic bacteria were increased remarkably when the iron was added to the digester in the form of mixture with substrates.

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Absorption of Water by Husked and Naked Barley (겉보리 및 쌀보리의 수화속도)

  • Lee, Jong-Sook;Kim, Sung-Kon
    • Korean Journal of Food Science and Technology
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    • v.15 no.3
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    • pp.220-224
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    • 1983
  • The hydration of water, at $20^{\circ}\;30^{\circ}$ and $40^{\circ}C$ for 10-360 minutes, by the two varieties of husked barley and of naked barley which were polished to 40 and 30%, respectively, was investigated. The absorption was directly proportional to the square root of the hydration time and accounted for by the diffusion equation: 1-M = $(2/{\sqrt\pi})\;(S/V){\sqrt{Dt}}$, where 1-M is the relative moisture gain and S/V is the surface-to-volume ratio. The average diffusion coefficient (D) was given by the Arrhenius relation: D = $D_{0}\;exp\;(-Ea/RT)$, where the activation energy for both husked and naked barley was about 7.2 Kcal/mole. The average value of D for naked barley was slightly higher than that for husked barley.

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Screening and Characterization of $\alpha$-Amylase Inhibitors from Cereals and Legumes in Korea (한국산 곡류와 두류 중 $\alpha$-Amylase 저해물질의 검색 및 특성)

  • Sim, Gi-Hwan;Bae, Yeong-Il;Mun, Ju-Seok
    • Food Science and Preservation
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    • v.1 no.2
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    • pp.117-124
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    • 1994
  • To investigate characterization of the ${\alpha}$-amylase inhibitors from cereals and legumes produced in Korea, inhibitory activities against ${\alpha}$-amylase with the inhibitor from barley(Hordeum vulgare), wheat(Triticum aestivun), black bean(Glycine max), bean(Cajanus cajon) and pea(Pisum sativum) were measured. Among the samples tested, inhibitors from naked barley and black bean(sabong) which showed the highest inhibitor activities of cereals and legumes, respectively, were characterized according to treatment condition. The results obtained were summarized as follows. During the germination of naked barley and black bean, ${\alpha}$-amylase activities were gradually increased but inhibitory activities against ${\alpha}$-amylases were decreased. Both activities were gradually decreased when naked barley and black bean were stored. More than 50% of activities of the inhibitors from naked barley and black bean were remained at 100$^{\circ}C$ for 15 min and 20 min, respectively, indicating that the inhibitor from black bean was more stable to heat than that of barley.

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Studies on the Properties of Barley and Naked Barley Starch Part II. On the Gelatinization Temperature and Alkali Number of Starch (보리전분(澱粉)의 특성(特性)에 관한 연구(硏究) 제2보 보리 전분(澱粉)의 호화온도(糊化溫度) 및 Alkali 수(數)에 대하여)

  • Kim, Yong-Hui;Kim, Hyong-Soo
    • Korean Journal of Food Science and Technology
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    • v.8 no.1
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    • pp.42-46
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    • 1976
  • In order to compare the quality of starches, isolated from the various barley and naked barley species, the gelatinization-temperature and alkali number were determined for 11 species of barley and 13 species of naked barley. The results are as follows; 1. Gelatinizations start at $51-59^{\circ}C$ and complete at $58-64^{\circ}C$ in range. Average gelatinization temperature of the starches from naked barley showed $3^{\circ}C$ lower than those from barley while small differences were observed between species for both barleys. 2. Alkali number varies between 8.0 to 9.5. No significant changes of alkali number were observed between both barleys (8.8 for naked barley and 8.7 for barley in average).

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Treatment of Distillery Wastewater Using a Thermophilic High-Rate Hybrid Anaerobic Reactor in Industrial Scale

  • Nam, Ki-Du;Chung, In;Young, James C.;Park, Wan
    • Journal of Microbiology and Biotechnology
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    • v.9 no.6
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    • pp.737-743
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    • 1999
  • A conventional thermophilic anaerobic digester was converted into a thermophilic high-rate hybrid anaerobic reactor (THAR) for treating distillery wastewater. The THAR has been operating successfully since May 1995 at a loading rate of 5.45 to $11.52{\;}kg/\textrm{m}^3/d$ (maximum of 15.02). The THAR has demonstrated a soluble Chemical Oxygen Demand (sCOD) removal efficiency of 85 to 91% and a total COD (tCOD) removal efficiency of as much as 72 to 84%. Product gas had a methane content of 59 to 68%. The tCOD removal rates were 4.31 to 5.43, 6.26 to 6.89, and 9.03 to $9.78kg{\;}tCOD/\textrm{m}^3/d$ for tapioca, com, and naked-barley wastewater, respectively. The sCOD removal rates ranged from 3.75 to 4.79,3.28 to 4.89, and 5.57 to 6.21kg $sCOD/\textrm{m}^3/d$ for tapioca, com, and naked-barley wastewater, respectively. There were unknown substances in a naked-barley distillery wastewater that were identified as being toxic for microorganisms. However, the THAR treated naked-barley wastewater continuously for 26 days, operating at an average tCOD loading of $11.08{\;}kg/\textrm{m}^3/d$without any signs of deterioration in either COD removal efficiency or gas production rate. During this period, the average removal efficiencies of tCOD and sCOD were 84% and 91%, respectively, and the gas production rate averaged 6.61 to $7.57{\;}\textrm{m}^3/\textrm{m}^3$ reactor/d which produced 0.57 to $0.69{\;}\textrm{m}^3{\;}biogas/kg{\;}tCOD_{rem}$. From tapioca and com wastewater, the reactor showed an average gas production rate of 3.18 to 3.46 and 4.91 to $5.22{\;}\textrm{m}^3/\textrm{m}^3$ reactor/d which produced 0.53 to 0.69 and 0.62 to $0.71{\;}\textrm{m}^3/kg{\;}tCOD_{rem}$, respectively.

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Varietal and Annual Variations of β-Glucan Contents in Korean Barley (Hordeum vulgare L.) and Oat (Avena sativa L.) Cultivars

  • Lee, Mi-Ja;Yoo, Jae-Soo;Kim, Yang-Kil;Park, Jong-Chul;Kim, Tae-Soo;Choi, Jae-Seong;Kim, Kee-Jong;Kim, Hyung-Soon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.3
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    • pp.284-291
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    • 2011
  • Varietal and annual variations in the contents of ${\beta}$-glucan fractions per weight grain samples were examined in sixteen covered and eighteen naked barley and five oat cultivars developed in Korea. Also, the effect of pearling on ${\beta}$-glucan content was investigated. Average contents of total, soluble and insoluble ${\beta}$-glucan fractions were 5.25, 3.72, and 1.53%, respectively, in covered barley, and 5.86, 3.51, and 2.35%, respectively, in naked barley. Soluble ${\beta}$-glucan content was higher in covered barley, though total ${\beta}$-glucan content higher in naked barley. The total and insoluble ${\beta}$-glucan contents were higher in pearled grains. Total ${\beta}$-glucan content was higher in waxy barley than in non-waxy barley. Duwonchapssalbori, a two-rowed and waxy naked barley cultivar, was highest in total, soluble and insoluble ${\beta}$-glucan contents. Highly significant positive correlations were observed between total ${\beta}$-glucan and soluble ${\beta}$-glucan contents both in covered and naked barley. There were significant annual variations in total ${\beta}$-glucan content in barley. Average contents of total, soluble and insoluble ${\beta}$-glucans of oat cultivars were 4.33, 3.44, and 0.89%, respectively. Contents of all fractions of ${\beta}$-glucans were higher in barley than in oat. These results would be useful for the breeding of high ${\beta}$-glucan variety and also for the use barley and oat as valueadded food ingredients.

Breeding of a New Non-Cocooning Silkworm Variety, Hachojam, Suitable for Autumn Rearing Season

  • Kang, Pil-Don;Sohn, Bong-Hee;Lee, Sang-Uk;Hong, Sung-Jin
    • International Journal of Industrial Entomology and Biomaterials
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    • v.4 no.1
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    • pp.77-81
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    • 2002
  • A new non-cocooning silkworm variety, Hachojam, suitable for autumn rearing season is single cross Fl hybrid between Japanese race Jam 307 and Chinese race Jam 126. Jam 307, Japanese parent of the Hachojam, which is a source for non-cocooning process showed a high GCA (generation combining ability) in naked pupation rate and Jam 126, Chinese parent, showed a high GCA in pupation rate and single pupal weight. In the local adaptability test performed at 8 local areas in autumn of 1999 to 2000, the naked pupation rate and thin cocoon rate of Hachojam were 64.7% and 35.3% , respectively. The pupal weight calculated from 10,000 of the 3rd molted larvae was 24% heavier in Hachcjam than the cocoon-producing, check variety Daesungjam.

The Soil and Mineral Nutrient Erosion on the Floors of Vegetations (식피에 따른 토양과 무기양분의 유실)

  • 장남기;윤성모
    • Asian Journal of Turfgrass Science
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    • v.8 no.3
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    • pp.149-165
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    • 1994
  • This report was investigated on the soil and mineral nutrient erosions in comparison among the vegetation floors of M isranthus sisensis, Arternisia stelleriana, Rhododendron mucronula turn, Zoysia ja-ponira and Pinus deusitlora communities and the naked soil on Mt. Keum-hak in Cheolwon-Koon, The erosion of clay, silt, fine sand and coarse sand of the surface soils under the Z. japonira grass-land was far less then those of M. sinensis, A. stelleriana, R. murronulatum and P. densiflora com-munities and the naked soil, The loss of mineral nutrients due to the soil erosion was the lowest level in the Z. japonira grassland and the highest level in the P. densiflora forest and the naked soil, respectively.

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