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CAM 플라스미드를 함유하는 Pseudomonas putida PpG1에서 TOL 플라스미드이 DNA 재배열 (DNA Rearrangement of TOL Plasmid in Pseudomonas putida PpGl Harbouring CAM Plasmid)

  • 전효곤;조경연;고영희
    • 한국미생물·생명공학회지
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    • 제18권4호
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    • pp.433-436
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    • 1990
  • 접합에 의한 P.putida mt-2의 TOL를 CAM 함유 P.putida PpG1으로 이동시켜 형성된 접합주 P.Putida CST3A는 작아진 TOL(TOL$\Delta$)를 가지고 있었지만, m-toiuate를 분해할 수 있었다. 접합에 의한 이동실험은 CAM에 결합되어 CAM:TO* 플라스미드를 형성하고 있었다. 불화합성 Inc P9군에 속하는 NAH를 CAM:TOL* 과 TOL$\Delta$을 가지는 P.putida CST3A로 이동시키면 TOL$\Delta$의 방출이 관찰되었으나 m-toluate 대사에는 아무런 영향을 미치지 않았다.

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Enhancing the Reconstruction of Acoustic Source Field Using Wavelet Transformation

  • Ko Byeongsik;Lee Seung-Yop
    • Journal of Mechanical Science and Technology
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    • 제19권8호
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    • pp.1611-1620
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    • 2005
  • This paper shows the use of wavelet transformation combined with inverse acoustics to reconstruct the surface velocity of a noise source. This approach uses the boundary element analysis based on the measured sound pressure at a set of field points, the Helmholtz integral equations and wavelet transformation for reconstructing the normal surface velocity field. The reconstructed field can be diverged due to the small measurement errors in the case of nearfield acoustic holography (NAH) using an inverse boundary element method. In order to avoid this instability in the inverse problem, the reconstruction process should include some form of regularization for enhancing the resolution of source images. The usual method of regularization has been the truncation of wave vectors associated with small singular values, although the order of an optimal truncation is difficult to determine. In this paper, a wavelet transformation is applied to reduce the computation time for inverse acoustics and to enhance the reconstructed vibration field. The computational speed-up is achieved, with solution time being reduced to $14.5\%$.

Degradation Properties of n-Alkane Assimilating Pseudomonas putida 3SK Carrying $CAM::TOL^{*}$ Plasmid and NAH Plasmid

  • Chun, Hyo-Kon;Cho, Kyung-Yun;Kho, Yung-Hee
    • Journal of Microbiology and Biotechnology
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    • 제4권4호
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    • pp.270-273
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    • 1994
  • Pseudomonas putida 3SK, which was constructed by the conjugal transfet of the $CAM::TOL^{*}$ plasmid of Pseudomonas putida CSnA and the NAH plasmid of Pseudomonas putida KCTC 2403 into n-alkane assimilating Pseudomonas putida KCTC 2405, showed a broad degradation spectrum and floc-forming ability. This strain degraded m-toluic acid, naphthalene, camphor and decane simultaneously. $Hg^{2+}$ at the concentration of 1 ppm in the minimal medium could not inhibit the growth of this strain. The degradation of m-toluic acid by Pseudomonas putida 3SK was not repressed by the easily utilizable compounds, such as glucose and succinate. But, the addition of formalin inhibited the growth of Pseudomonas putida 3SK. After the cultivation of this strain on the artificial wastewater containing m-toluic acid, naphthalene, camphor and decane for 24 hr, the initial COD value (1500) of the artificial wastewater was declined to 300.

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EXPERIMENTAL RESEARCH ON VIBRATING PLATE AND ITS RADIATION FIELD USING NEARFIELD ACOUSTIC HOLOGRAPHY

  • D.J. Zhang;J.Z. Cheng;N.Q. Zuh;Q. Wang;X.H> Xia
    • 한국음향학회:학술대회논문집
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    • 한국음향학회 1994년도 FIFTH WESTERN PACIFIC REGIONAL ACOUSTICS CONFERENCE SEOUL KOREA
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    • pp.842-847
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    • 1994
  • This paper presents the nearfield acoustic holography (NAH) imaging system for airborne sound constructed in our own laboratory. The effects of different kinds of noise and the filter function's form in wave number space for reconstructions are analysed emphatically. Using the system we have measured the vibrating mode and the radiated field of a simulative "Chime Stone" made by metal, and gotten interesting results. These results indicate that NAH can be used to research the mechanism of sound production and evalution of musical quality for Chime Stone as an effective means.

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한국 젊은 성인의 정규화된 아치 높이에 따른 발 유형 분석 (Analysis of Foot Type in Korean Young Adults Based on Normalized Arch Height)

  • 정도영
    • 한국전문물리치료학회지
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    • 제27권3호
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    • pp.199-205
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    • 2020
  • Background: The classification of foot type can be commonly determined by the height of the media longitudinal arch. The normalized arch height (NAH) is defined as the ratio of navicular or instep heights to the foot length or instep length. Objects: This study investigated the relationships among foot characteristics, such as foot length (FL), instep length (IL), navicular height (NH), and instep height (IH), in Korean young adults. Also, the distribution of foot type based on calculated NAH was assessed. Methods: Three-dimensional foot scanning data of young adults aged 20 to 39 years (total: 1,978; 974 male, 1,004 female) were obtained from the Korea Technology Standards Institute, and used for analyses. NAH was calculated as the following: NH/FL, IH/FL, IH/IL, NH/IL. Spearman's rank order correlation was used to identify correlations among variables. The Mann-Whitney U-test and chi-square test were used to compare the sex differences in foot characteristics and distribution of foot type. Results: FL and IL showed a very high correlation (r = 0.94). The correlations between FL or IL and IH (r = 0.50-0.57) were greater than those between FL or IL and NH (r = 0.23-0.72). Males had significantly larger values than females (p < 0.001), and the frequency of pes planus was significantly higher in females than in males (χ2 = 50.09, p < 0.001). Based on the IH/IL index, the neutral foot, pes planus and pes cavus distributed by 16%, 78%, and 6% respectively. Conclusion: Our results on foot arch distribution could be used as basic data in clinical or footwear fields, and our data on differences in arch structure according to sex may facilitate understanding of why injury to the lower limbs differs between males and females.

Evidence of Indigenous NAB Plasmid of Naphthalene Degrading Pseudomonas putida PpG7 Strain Implicated in Limonin Degradation

  • Ghosh, Moushumi;Ganguli, Abhijit;Mallik, Meenakshi
    • Journal of Microbiology
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    • 제44권5호
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    • pp.473-479
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    • 2006
  • A well characterized naphthalene-degrading strain, Pseudomonas putida PpG7 was observed to utilize limonin, a highly-oxygenated triterpenoid compound as a sole source of carbon and energy. Limonin concentrations evidenced a 64% reduction over 48 h of growth in batch cultures. Attempts were made to acquire a plasmid-less derivative via various methods (viz. Ethidium Bromide, SDS, elevated temperature & mitomycin C), among which the method involving mitomycin C ($20{\mu}g/ml$) proved successful. Concomitant with the loss of plasmid in P. putida PpG7 strain, the cured derivative was identified as a $lim^-$ phenotype. The $lim^+$ phenotype could be conjugally transferred to the cured derivative. Based on the results of curing with mitomycin C, conjugation studies and presence of ndo gene encoding naphthalene 1,2 dioxygenase, it was demonstrated that genes for the limonin utilization were encoded on an 83 kb indigenous transmissible Inc. P9 NAH plasmid in Pseudomonas putida PpG7 strain.