• 한국자원식물학회지
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• 제17권3호
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• pp.278-282
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• 2004

한방에서 당귀로 쓰여지고 있는 참당귀(Angelica gigas)와 일당귀 (A. acutiloba)의 추출물로 3종의 그람양성균, 2종의 그람음성균과 1종 효모에 대한 항균활성을 조사한 결과는 다음과 같다. 참당귀 지상부와 지하부 추출액의 항균활성은 ethyl acetate 분획물에서만 나타났는데 지상부 추출액에서는 그람양성균인 S. aureus에 대한 항균활성이 가장 강했고, 지하부 추출물에서는 그람양성균인 B. subtilis와 그람음성균인 E. coli에 대한 항균활성이 가장 강했다. 일당귀 추출액의 항균활성은 지상부 추출액은 n-hexane 분획물에서 지하부 추출액 은 water분획물에서 전혀 나타나지 않았으며 지상부와 지하부 추출액의 ether분획물에서 가장 강했다. 효모 S. cerevisiae에 대해서는 항균환성이 전혀 나타나지 않았다.

• 한국식품과학회지
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• 제34권2호
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• pp.274-282
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• 2002

물레나물 에탄올 추출물과 헥산 분획물은 L. monocytogenes 5균주에 대해 25 ppm에서 72시간까지 완전 균증식 억제 효과가 있었다. 물레나무 헥산 분획물의 항균활성 물질을 분리, 정제하여 얻은 H2-5-2 소획분의 경우 실험 MIC가 10 ppm으로 그 항균력을 생균수로 나타내었을 때 초기 접종 균수보다 0.5 log cycle정도 감소하여 그 살균효과를 알 수 있었다. 물레나물 헥산 분획물의 항균 스펙트럼을 살펴보면 B. cereus나 S. aureus에 대해서는 실험 최저 농도인 25 ppm에서도 72시간까지 완전 증식억제 효과를 나타내었으며, V. parahaemolyticus에 대해서는 50 ppm 첨가시 72시간 동안 균증식이 억제되었다. 물레나물의 항균활성 물질은 불포화도가 높은 sterol로 추정되었다. 쇠고기와 명태육 마쇄물에 물레나물 헥산 분획물을 첨가하여 $32^{\circ}C$ 와 $5^{\circ}C$에서 식품 적용 실험을 실시한 결과 $32^{\circ}C$ 저장온도에서는 쇠고기, 명태육에 첨가한 물레나물 헥산 분획물의 항균효과가 나타나지 않았고, $5^{\circ}C$ 저장온도에서는 명태육에 첨가된 물레나물 헥산 분획물은 항균효과가 있었다. 그러나 물레나물 헥산 분획물의 항균효과는 배양액 상태보다 감소하였다.

• 한국균학회지
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• 제45권3호
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• pp.175-187
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• 2017

Laetiporus sulphrueus var. miniatus is widely distributed worldwide, and has commonly been used as a medicinal mushroom. In the present study, we investigated the effects of water extract and solvent fractions from the Laetiporus miniatus as possible antioxidant, anti-thrombin and anti-invasive agents against phorbol 12-myristate 13-acetate (PMA)- or thrombin-induced matrix metalloproteinase-2 (MMP-2) and MMP-9 activities. Samples were fractionated into n-hexane, $CHCl_3$, ethyl acetate, n-butanol, and water fractions, and individually analysed. The water fraction had the highest extraction yield at 34.90% (w/w), while the n-butanol fraction demonstrated the highest anti-oxidative activity at 81.44%. In the thrombin inhibitory activity test, the water fraction exhibited the highest activity at 94.64%. Even at the concentration of $40{\mu}g/mL$, evaluation of anti-proliferating activity in YD-10B cells did not reveal any cytotoxic effects. Although MMP-9 expression in YD-10B cells increased after the addition of PMA and thrombin, MMP-2 did not. Additionally, MMP-2/-9 levels in PMA-treated YD-10B cells (i.e., both mRNA expression and protein activation) were highly inhibited in the hexane and chloroform fractions. Compared with MMP-2 levels, MMP-9 mRNA expression and proteolytic activity were inhibited to a greater extent by the hexane and chloroform fractions in thrombin-treated YD-10B cells. Taken together, these results support that thrombin induces tumor invasion through MMP-2/9 and suggest that the L. miniatus may act as an effective functional food, conferring anti-oxidative, anti-thrombotic and anti-cancer activities.

• Journal of the Korean Wood Science and Technology
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• 제43권2호
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• pp.238-247
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• 2015

Katsura tree (Cercidiphyllum japonicum Sieb. Et Zucc) leaves were collected, air-dried and extracted with 70% aqueous acetone, then concentrated and sequentially fractionated using n-hexane, $CH_2Cl_2$, EtOAc, and $H_2O$ to be freeze dried for antioxidant and ${\alpha}$-glucosidase inhibition activity tests. The antioxidant activity of the extracts was evaluated using DPPH (1,1-diphenyl 2-picrylhydrazyl) free radical scavenging assay. The test concentrations were adjusted to 500, 250, 125, 62.5, 31.25, 15.62, 7.81, 3.9, 1.95 and 0.97 ppm. The $H_2O$ and EtOAc fractions showed higher activities compared with the control, ${\alpha}$-tocopherol, at all concentrations. The crude fraction also gave better activity at the concentrations lower than 62.5 ppm. However, the nonpolar n-hexane and $CH_2Cl_2$ fractions gave prominently lower activities compared with the control at all concentrations. The $IC_{50}$ values of the crude, EtOAc, and $H_2O$ fractions exhibited 11.78, 4.29 and $9.80{\mu}g/m{\ell}$, respectively, compared with $12.08{\mu}g/m{\ell}$ of the control. But the n-hexane and $CH_2Cl_2$ fractions indicated 300 and $91.85{\mu}g/m{\ell}$ of $IC_{50}$, respectively. ${\alpha}$-Glucosidase inhibition activity was evaluated at the concentrations of 50, 25, 12.5, 6.3, 3.1, 1.6 and 0.8 ppm. The inhibition activities were increased according to as the increase of sample concentrations. However, the nonpolar n-hexane and $CH_2Cl_2$ fractions indicated very low inhibition activities compared with acarbose, a positive control. The EtOAc fraction showed very good capability as almost 100% compared with the control at the higher concentrations than 12.5 ppm and the crude fraction also indicated good potential as 95% and 100% at 25 and 50 ppm, respectively. The $H_2O$ fraction gave good inhibition value as 90% at 50 ppm although the value was lower than the control. These results showed that the polar fractions had better ${\alpha}$-glucosidase inhibition activities. The $IC_{50}$ values of the nonpolar fractions, n-hexane and $CH_2Cl_2$, showed very lower values as 468 and $103.6{\mu}g/m{\ell}$, respectively, than the control. ${\alpha}$-Glucosidase Inhibition Activity of the Extracts of Katsura Tree (Cercidiphyllum japonicum Sieb. Et Zucc) Leaves However, the polar fractions, crude, EtOAc and $H_2O$, showed 7.1, 3.7 and $13{\mu}g/m{\ell}$, respectively, indicating that these fractions can be used as natural bioresources for treating diabetes mellitus. Also ${\alpha}$-glucosidase inhibition activity had a positive correlation with antioxidant activity of the extracts.

• 한국수산과학회지
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• 제43권5호
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• pp.437-444
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• 2010

Extracts of the marine microalgae Nannochloropsis oculata were obtained using 80% methanol (MeOH) and water. The 80% MeOH extract was further fractionated with n-hexane, chloroform, ethyl acetate (EtOAc), n-butanol (n-BuOH), and water to isolate the active fraction. Seven samples were prepared and their angiotensin converting enzyme (ACE), $\alpha$-glucosidase, and cancer cell growth inhibitory activities in vitro were determined. The most profound ACE inhibitory activity was observed in the chloroform fraction, while the others had moderate effects. By contrast, greater $\alpha$-glucosidase inhibitory activity was found in the EtOAc fraction, n-hexane fraction, and water extract of N. oculata. The antiproliferative effects of the extracts and fractions against HL-60, U937, CT-26, and SK-Hep1 cancer cells were also determined. The n-BuOH fraction had the strongest antiproliferative effects on CT-26 cells in a time-dependant manner (P<0.05). These results suggest that the extracts and fractions from N. oculata could be used as a potential functional food or as pharmaceutical ingredients.

• 한국식품과학회지
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• 제52권4호
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• pp.350-356
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• 2020

본 연구에서는 왕벚나무 껍질 에탄올 추출물과 분획물의 항산화물질 및 항산화활성을 측정하였다. 왕벚나무 껍질 에탄올 추출물 및 분획물의 총 페놀 함량은 ethyl acetate 분획물이 496.80 mg GAE/g으로 가장 높았고, 총 플라보노이드 함량은 7.26-265.52 mg RE/g으로 ethyl acetate 분획물이 가장 높게 나타났다. 프로안토시아니딘 함량은 ethyl acetate, n-buthanol, ethanol, dichloromethane, water, n-hexane 순으로 높았다. DPPH, ABTS⁺, superoxide^- 라디칼 소거능 및 환원력은 ethyl acetate 분획물의 활성이 가장 좋았고, 아질산염 소거능에서는 에탄올 추출물이 가장 높은 활성을 나타냈다. 반면에 n-hexane과 water 분획물은 항산화물질의 함량이 낮고 항산화활성이 다른 시료에 비해 낮게 측정되었다. 따라서 왕벚나무 껍질 ethyl acetate 분획물은 항산화물질이 풍부하고 높은 항산화활성을 나타내어 천연 항산화제로 활용될 수 있을 것으로 기대된다.

• Preventive Nutrition and Food Science
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• 제16권4호
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• pp.376-380
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• 2011

We investigated the total phenolic and flavonoid contents and the antimicrobial activity of ethanol extracts obtained from Saussurea lappa C.B. Clarke. The ethanol extracts of S. lappa C.B. Clarke were fractionated with various solvents (n-hexane, chloroform, and n-butanol). The antimicrobial activity of S. lappa C.B. Clarke was examined by disc-diffusion and micro-dilution susceptibility assays with six food-borne pathogens, and compared to that of the synthetic antibiotics. It is found that the S. lappa C.B. Clarke ethanol extract and n-hexane fraction have strong activity against B. cereus and V. parahaemolyticus strains compared to ampicillin. The inhibitory concentration ($IC_{50}$) values of hexane fraction against L. monocytogenes, B. cereus, and B. subtilis were 62.5, 250 and 500 ppm, respectively. Therefore, these data suggest that S. lappa C.B. Clarke may be useful as antimicrobial agents against food-borne pathogens.

• Journal of Forest and Environmental Science
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• 제23권1호
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• pp.1-4
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• 2007

The bark of Juglans mandshurica Maxim. was collected, extracted with acetone-$H_2O$ (7:3, v/v), fractioned with n-hexane, $CH_2Cl_2$ and EtOAc, then each fraction was freeze-dried to give some powders. A portion of the EtOAc (28.4 g) fraction was chromatographed on a Sephadex LH-20 column eluting with a series of MeOH-$H_2O$ and EtOH-hexane mixture. Four gallotannins, gallic acid (1), ellagic acid (2), 1,2,4,6-tetra-O-galloyl-${\beta}$-D-glucose (3) and 1,2,3,4,6-penta-O-galloyl-${\beta}$-D-glucose (4), have been isolated from the EtOAc fraction Their structures were elucidated on the basis of chemical evidence and spectrometric analysis such as NMR and MS. The antioxidant activities on each fraction and the isolated gallotannins were evaluated by DPPH radical scavenging test.

• Journal of the Korean Wood Science and Technology
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• 제37권5호
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• pp.480-485
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• 2009

Juglans mandshurica bark was collected and extracted with 70% aqueous acetone. The extracts were concentrated and then sequentially fractionated using n-hexane, $CH_2Cl_2$, EtOAc, and $H_2O$ to be freeze dried. A portion of $H_2O$ fraction was chromatographed on a Sephadex LH-20 column using aqueous methanol to isolate (S)-2,3-HHDP-D-glucopyranoside (1). Some of EtOAc fraction was also treated on a Sephadex LH-20 column using aqueous methanol and EtOH-hexane mixture to purify pedunculagin (2). The structures of these two epimeric ellagitannins were elucidated by spectroscopic tools, NMR and MS.

• 생명과학회지
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• 제29권3호
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• pp.337-344
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• 2019

본 연구에서는 택란 잎에 대한 다양한 생리활성을 규명하기 위하여 택란 잎을 극성이 다른 용매로 분획하여 분획물의 플라보노이드 함량을 측정하고 택란 잎 분획물에 의한 라디칼 소거활성과 세포독성 효과를 규명하고자 하였다. 택란 잎 85% MeOH 및 n-BuOH분획물들의 총 플라보노이드 함량은 각각 $41.5{\pm}0.00mg/g$ 및 $77.2{\pm}0.01mg/g$으로 n-BuOH 분획물의 총 플라보노이드 함량이 가장 높았음을 알 수 있었다. DPPH assay에서 택란 잎 n-BuOH 분획물의 활성산소 소거능이 가장 우수하였고 다음으로 85% MeOH 분획물에 의한 소거활성이 높았다(p<0.05). 각 분획물들의 $IC_{50}$값은 n-Hexane 9.76 mg/ml, 85% MeOH 0.50 mg/ml, n-BuOH 0.03 mg/ml 및 water 0.78 mg/ml로 나타났다. 또한 택란 잎 85% aq. MeOH 및 n-BuOH 분획물들에 의한 ABTs+소거활성은 각각 89.3% 및 92.1%이었으며 대조군인BHT와 L-ascorbic acid와 유사한 값을 나타내었다(p<0.05). ABTs+ assay에서 각 분획물들의 $IC_{50}$값은 n-Hexane 0.96 mg/ml, 85% MeOH 0.16 mg/ml, n-BuOH 0.01 mg/ml 및 water 0.31 mg/ml로 나타났다. 택란 잎 n-Hexane, 85% MeOH, n-BuOH 및 water 분획물들은 농도의존적으로 암세포들에 대한 세포독성 효과를 나타내었으며 분획물들 중 특히 n-Hexane과 85% MeOH 분획물들에 의한 세포독성 효과가 가장 높았다. AGS 암세포에 대한 택란 잎 n-Hexane, 85% MeOH, n-BuOH 및 water 분획물들의 $IC_{50}$ 값은 각각 0.18, 0.03, 0.62 및 0.12 mg/ml이었고 HT-29 암세포에 대한 $IC_{50}$ 값은 각각 0.26, 0.16, 0.60 및 1.14 mg/ml이었다. HT-1080 암세포에서 택란 잎n-Hexane, 85% MeOH, n-BuOH 및 water 분획물들의 $IC_{50}$ 값은 각각 0.34, 0.14, 0.48, 및 1.05 mg/ml이었다. 따라서 본 연구결과로부터 택란 잎 n-BuOH 분획물은 자유라디칼 소거활성에 효과적이었고 n-Hexane과 85% MeOH 분획물들은 높은 세포독성 효과를 나타내었으며 향후 택란 잎을 이용한 식품개발을 위한 기초자료를 제시하고자 한다.