• 생명과학회지
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• 제22권2호
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• pp.200-208
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• 2012

본 연구에서는 균사의 액체 배양 특성 조사를 위하여 꽃송이버섯 균주 KGFS08과 KFRI746를 선발하였다. 균사 생장을 위한 우수 배지는 KTM 배지로 조사되었으며, 탄소원과 질소원은 각각 3% (w/v) starch와 0.3% (w/v) tryptone, 비타민은 folic acid로 나타났다. 따라서 액체 배양 최적 조건은 STK 배지[3% (w/v) starch, 0.3% (w/v) tryptone, 0.1% (w/v) $KH_2PO_4$, 0.1% (w/v) folic acid], pH는 4.0~5.0으로 확인되었다.

• 한국버섯학회지
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• 제19권1호
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• pp.1-8
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• 2021

The choice of the culture medium is an important factor for the mass production of mycelia in submerged cultures. The influence of liquid medium on the mycelial dry weight of Paecilomyces tenuipes was investigated in this study. The regression equation is expressed as Y=-1292.94187+17.78612X1+18.92425X2+2.11464X3-0.019375X1X2-0.006276X1X3+0.008177X2X3-0.070169X12-0.292175X22-0.008818X32, where Y represents the value of the mycelial dry weight (g/L), X1 is the particle size of wood sawdust in liquid medium (mesh), X2 is the concentration of the wood sawdust in liquid medium, and X3 is incubation time (h). The medium was optimized using a response surface methodology, and the optimal medium contained 30 g of wood sawdust (140 mesh), 20 g of glucose, and 10 g/L of peptone. Under these conditions, the mycelial dry weight reached 38.1 g/L (actual value). The culture medium containing wood sawdust is simple and easy to use, highly efficient, and eco-friendly, and its effectiveness in large preparations of P. tenuipes mycelia with low material costs has been demonstrated.

• Mycobiology
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• 제35권2호
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• pp.54-61
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• 2007

Two isolates of Tricholoma matsutake T-008 and T-034, preserved in Entomopathogenic Fungal Culture Collection (EFCC) of Korea, were used in the present study. The isolates had 100% Bootstrap homology with Tricholoma matsutake U62964 and T. matsutake AB188557 and AF309538 preserved in Gene Bank of NCBI. Mycelial growth of T. matsutake was highest in TMM and MYA at $25^{\circ}C$. The highest dry wt. of mycelium was obtained after 65 days of culture, when 6 mycelial discs were inoculated in 100 ml of broth in 250 ml shaking flask. Mycelial mats were observed in clumped condition at the inoculation sites of pine forest after two weeks of inoculation. After 5 months of inoculation, mycelia mats were observed growing inside soil and walls of a few inoculation sites, while mycelial mats growth up to $5{\sim}8$ cm were observed in the roots of pine tree after 6 months. The survival rate of the inoculum was about 40% of the total inoculation sites. The survival rate was found below 20% when the mycelium was inoculated in the summer. The reasons for low survival rates of the mycelium were mainly due to dry season and the soil-borne small animals such as earthworm and mole. After one year of inoculation, no external difference was observed between the artificially inoculated mycelia and the naturally existing mycelia of T. matsutake. The present study showed that fruiting bodies of T. matsutake could be produced by artificial inoculation under the appropriate environmental conditions.

• Mycobiology
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• 제47권1호
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• pp.97-104
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• 2019

Mushroom cultivation has gained increased attention in recent years. Currently, only four types of spawn, including sawdust spawn, grain spawn, liquid spawn, and stick spawn, are commonly available for mushroom cultivation. This limited spawn diversity has led to difficulty in selecting suitable inoculum materials in some cultivation. In this study, three small blocks of lignocellulosic agro-wastes and one block of a synthetic matrix were prepared as support for growing Pleurotus ostreatus in liquid medium. Mycelium-adsorbed blocks were then evaluated for their potential as block spawn for fructification. Our results indicated that the edible fungus was adsorbed and abundantly grew internally and externally on loofah sponge and synthetic polyurethane foam (PUF) supports and also has the ability to attach and grow on the surface of sugarcane bagasse and corncob supports. The mycelia of P. ostreatus adhered on corncob exhibited the highest metabolic activity, while those on the PUF showed the least activity. Mycelial extension rates of block spawns made of agro-waste materials were comparable to that of sawdust spawn, but the block spawn of PUF showed a significantly lower rate. No significant differences in cropping time and yield were observed among cultivations between experimental block spawns and sawdust spawns. Moreover, the corncob block spawn maintained its fruiting potential during an examined period of 6-month storage. The developed block spawn could be practically applied in mushroom cultivation.

• 한국균학회지
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• 제42권2호
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• pp.150-158
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• 2014

4종의 외생균근균이 MMN 배지상에서 7종 단소원에 대한 이용능력을 조사하였다. 60일간 순수배양한 결과, 다색벚꽃버섯(KFRI 1987), 향버섯, 접시껄껄이그물버섯(KFRI 1194), 송이(KFRI 1256)는 dextrin에서 균사 생장이 우수하였다. 다색벚꽃버섯, 향버섯(KFRI 1676), 접시껄껄이그물버섯은 pectin에서 균사 생장이 가장 낮았다. 송이(KFRI 1256)를 제외한 7개의 균주들은 heteroglycan(pectin)보다 homoglycan계 탄소원(starch, dextrin)의 이용률이 더 높은 것으로 조사되었다. 균 배양 후 배지의 pH 값은 배양 전보다 최소 1.1~최대 3.0 범위까지 감소하였다. 외생균근균 배양체의 형태적 특징을 조사한 결과, 모든 탄소원 시험배지에서 백색 균사체가 우세하였으나 탄소원의 종류에 따라 황색, 갈색, 자주색 계열의 다양한 균사체가 관찰되었다. 접시껄껄이그물버섯은 4~5종류의 탄소원에서 single colony의 형태로 생장한 반면, 이외 대부분의 균주들은 6종의 탄소원에서 multiple colony의 형태로 생장하였다.

• 한국미생물·생명공학회지
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• 제25권5호
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• pp.501-505
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• 1997

The effects of some nutrients and culture conditions on the growth and the production of fumonisin B$_{1}$, (FB$_{1}$) from Fusarium moniliforme NRRL 13569 were investigated in liquid culture. Xylose and soytone yielded the highest mycelial growth as the C- and N-source, respectively. The highest level of FB$_{1}$, was obtained when yeast extract was used as the N-source but no FB$_{1}$, from NaNO$_{3}$. While Fe$^{+++}$ showed inhibition effect on FB$_{1}$, production, Zn$^{++}$ enhanced the FB$_{1}$, production as well as the mycelial growth. FB$_{1}$, was maximally produced when the initial pH value and the specific surface area of the medium was adjusted to 5 and 1.4 cm$^{2}$/ml, respectively. FB$_{1}$ formation reached the maximum value (210, 000 ng/ml) in 30 days and then decreased in Czapek medium substitued with 1% xylose and 0.3% yeast extract, and supplemented with 0.2% NH$_{4}$H$_{2}$PO$_{4}$ where the initial pH value and the specific surface area of the medium are optimally controlled.

• 한국균학회지
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• 제45권4호
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• pp.319-327
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• 2017

In this study, we investigated the culture conditions of four ectomycorrhizal fungi, namely, Amanita spissacea NIFoS 2719, Pisolithus arhizus NIFoS 2784, Suillus spraguei NIFoS 2848, and Xanthoconium affine NIFoS 2716, in solid and liquid culture media. In addition, we attempted to induce in vitro mycorrhization of the fungi with Pinus densiflora. Prior to liquid culture, we determined the optimal culture conditions for each species in solid media. The results revealed that all species examined are capable of growth in potato dextrose agar (PDA), malt extract agar (MEA), and modified Melin-Norkran's medium (MMN), although their preferred growth media were different. Liquid culture experiments showed that inorganic nitrogen did not enhance the mycelial growth of all four species. Therefore, we used MMN-based liquid inocula to promote the growth of ectomycorrhizal fungi in our symbiosis culture system. Mycorrhization was observed in Xanthoconium affine NIFoS 2716. Morphological analysis revealed that fungi-inoculated roots of P. densiflora form simple and dichotomous lateral roots with dense mycelia. In addition, inoculation with X. affine NIFoS 2716 promoted root and shoot developments.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.149-152
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• 2003

본 연구에서는 담자균류의 일종인 Tricholoma matsutake의 균사체 생육과 polysaccharide 생산을 최적화하기 위하여 5L jar fermentor에서 교반속도와 통기량의 영향을 검토하였다. 교반속도는 150 rpm에서 가장 좋은 결과를 보였으며, 균체량은 배양 10일째 21.87 g/L, polysaccharide는 교반속도는 300 rpm에서 배양 8일째 8.86 g/L 로 가장 높은 값을 나타내다. 통기량은 11일째 0.5 vvm에서 가장 좋은 균체량 20.85 g/L, polysaccharide는 1.5vvm에서 배양 11일째 8.83 g/L로 가장 높은 값을 나타내었다.

• 미생물학회지
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• 제42권2호
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• pp.83-88
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• 2006

국내에서 식용버섯으로 이용되고 있는 8종 균주의 자실체와 균사체로부터 콜레스테롤 합성을 저해하는 lovastatin 생산과 고지혈증 억제활성을 탐색하였다. TLC 분석으로 lovastatin생산 유무를 검색한 결과 자실체와 균사체의 추출물에 대해 Rf값이 표준물질콰 같은 약 0.46에서 뚜렷한 밴드가 형성됨을 확인하였다. 자실체로부터 lovastatin 추출을 위한 용매로는 water/acetonitrile/methanol (5:2.5:2.5)을 사용하였을 매 가장 효과적인 lovastatin 생산량을 보였다. Lovastatin 생산량은 자실체 중에서는 Pleurotus ostreatus가 0.98 mg/g (dry biomass)으로 가장 높은 생산량을 나타냈고 균사체 배양액 추출물에 대해서도 P. ostreatus가 21.90 mg/L로 높은 생산량을 나타냈다. 3-Hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase 저해활성에서는 자실체에서 P ostreatus가 67.8%, 균사체에서 P. ostreatus와Laetiporus sulphureus가 각각 37.2%, 29.1%의 저해활성을 보였다. L. sulphureus는 lovastatin 생산량에 비해 높은 저해활성을 나타내서 lovastatin 이외의 다른 고지혈증 억제물질을 GC/MS로 분석한 결과 대사산물로 분비되는 식물성 sterol 물질인 campesterol과 gamma-sitosterol이 검출되었다.

• 한국균학회지
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• 제28권2호
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• pp.81-87
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• 2000

곤충을 기주로 하여 자실체를 형성하는 곤충기생균(entomopathogenic fungi)의 일종인 풍뎅이동충하초(Cordyceps scarabaeicola Kobayasi)는 풍뎅이상과(Scarabaeoidea)의 성충을 기주로 하여 자실체를 형성한다(Evans, 1982; Evans and Samson 1982; Humber(unpublished); Kobayasi, 1940). 풍뎅이동충하초(C. scarabaeicola)는 자낭균아문(Ascomycotina), 핵균강(Pyrenomycetes)의 맥각균목(Clavicipitales), 동충하초과(Cordycipiteae), 동충하초속(Cordyceps)에 속하며 한국을 비롯하여 중국, 일본 등 전 세계적으로 분포한다(Dennis, 1981; Sung, et al, 1997; 청수. 1981). 또한 이(1996)는 풍뎅이동충하초의 불완전세대는 Beauveria속의 분생포자를 형성한다고 보고하였다. 풍뎅이동충하초는 MCM에서 잘 생장하나 균사밀도는 PDA배지가 가장 적합한 것으로 나타나 다른 버섯균과는 별다른 차이를 보이지 않았으며 온도는 $25^{\circ}C$가 가장 좋았으며 pH는 $7.5{\sim}9.0$으로 균주에 따라 차이를 보였다. 이러한 결과는 이(1996)의 연구결과인 PD배지와 $24^{\circ}C$에서 우수한 균사생장을 보였고 $30^{\circ}C$에서는 약간의 균사생장이 억제되었고, pH $8{\sim}9$의 알카리성 범위에서 우수한 균사생장을 보였다는 보고와 거의 일치함을 보였다. 풍뎅이동충하초는 탄소원을 첨가하지 않은 것에서 균사생장이 가장 높게 나타났지만 균사밀도는 가장 낮게 나타났다. 균사생장과 밀도는 lactose가 적합한 탄소원으로 나타났으며, 질소원은 ammonium phosphate에서 높은 균사생장을 보여 모두 무기태 질소원에서 우수함을 보였다.