• Journal of Microbiology and Biotechnology
• /
• 제10권4호
• /
• pp.482-487
• /
• 2000

Optimization of submerged culture conditions for the production of exo-biopolymer from Paecilomyces japonica ws studied. Maltose, yeast extract, and potassium phosphate were the most suitable sources of carbon, nitrogen, and inorganic salt, respectively, for both production of the exo-biopolymer and mycelial growth. The optimal culture conditions in a flask culture were pH 5.0, $25^{\circ}C$, and 150 rpm in a medium containing (as in g/l) 30 maltose, 6 yeast extruct, 2 polypeptone, $0.5{\;}K_3HPO_4,{\;}0.2{\;}KH_2PO_4,{\;}0.2{\;}MnSO_4{\cdot}5H_2O,{\;}0.2{\;}MgSO_4{\cdot}7H_2O$. Exo-biopolymer production and mycelial growth in the above suggested medium were significantly increased in a 2.5-1 jar fermentor, where the maximum biopolymer concentration was 8 g/l. The morphological changes of the mycelium in the submerged culture were observed within pH ranges from 4.0 to 9.0; i.e., growth of the filamentous form was optimal at culture pHs of 5.0 and 6.0, whereas pellet was formed at other pHs.

• 한국균학회지
• /
• 제37권2호
• /
• pp.173-180
• /
• 2009

This study was carried out to obtain basic data on mycelial growth characteristics for an artificial cultivation of Volvariella volvacea. Twelve URP primers were used to assess genetic relationship of V. volvacea and its result was divided into two groups. But there was nothing different in morphological characteristics in V. volvacea. Among five kinds of mushroom media, MCM medium was selected as the favorable culture medium. The optimal range of temperature and pH for mycelial growth on V. volvacea were 35oC and pH 6~8, respectively. Carbon sources had not an effect on 10 strains of V. volvacea, and nitrogen source for the optimum mycelial growth was yeast extract. Also, we selected GMVV 79004 as a superior strain of 10 strains V. volvacea based on the mycelial growth and yield.

• KSBB Journal
• /
• 제11권5호
• /
• pp.572-576
• /
• 1996

Conditions for the culture of Pleurotus spp. mycelium using rancid frying oils were investigated. Among the six strains tested, Pleurotus ostreatus CBS 03 showed the greatest mycelial growth on fish paste and ramyon frying oil, and was used in this study. The optimum temperature and pH for mycelial growth were from 25 to $30^{\circ}C$ and pH 5.5 to 6.0, respectively. Tryptone for mycelial growth was better than any other nitrogen sources. The addition of $KH_2PO_4 and MgSO_4$ enhanced mycelial growth at 0.2 and 0.01% on fish frying oil, and at 0.1 and 0.03% on ramyon frying oil. Among the vitamins used, thiamine and nicotinic acid were the most effective ones.

• Applied Biological Chemistry
• /
• 제6권
• /
• pp.57-59
• /
• 1965

According to the result of the observations on the effect of the reagents, $CuSO_4{\cdot}5H_2O$, $ZnSO_4{\cdot}7H_2O$ $MnSO_4{\cdot}4H_2O$, $HgCl_2$, upon the mycelial growth of psalliota campestris $HgCl_2$ showed the strongest effect on checking the mycelial growth $(,.008{\sim}0.01%)$ $CuSO_4{\cdot}5H_2O(0.030%)$, $ZnSO_4{\cdot}7H_2O(0.080%)$, came next and the $MnSO_4{\cdot}4H_2O(0.100%)$ was the weakest. Writer also found that showed the promoting effect of the mycelial growth in appropriate Concentration (at $0.015%\;CuSO_4{\cdot}5H_2O$, $0.030%ZnSO_4{\cdot}7H_2O$).

• Journal of Microbiology and Biotechnology
• /
• 제16권8호
• /
• pp.1240-1247
• /
• 2006

The present study investigated the influence of the aeration rate and agitation intensity on the production of the mycelial biomass and antioxidative exopolysaccharide (EPS) in Ganoderma resinaceum. In submerged cultures with varying agitation speeds and aeration rates in a stirred-tank reactor, the maximum mycelial biomass and maximum EPS concentration were achieved at 50 rpm and 300 rpm, respectively. Under varying aeration rates, the highest amount of mycelial biomass (18.1 g/l) was accumulated at the lowest aeration rate (0.5 vvm) and the maximum EPS production (3.0 g/l) obtained at 1.0 vvm. A compositional analysis revealed that the five different EPSs were protein-bound heteropolysaccharides, consisting of 87.17-89.22% carbohydrates and 10.78-12.83% proteins. The culture conditions had a striking affect on the carbohydrate composition of the EPS, resulting in different antioxidative activities. All the EPSs showed strong scavenging activities against superoxide and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radicals, whereas no clear trend in antioxidative activity was observed against hydroxyl radicals and lipid peroxides. Although the precise reason for this difference is still unclear, the high glucose moiety of EPS is probably linked to its broad spectrum of antioxidative activity.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2001년도 추계학술발표대회
• /
• pp.382-385
• /
• 2001

The effect of agitation speed on the production of mycelial growth and exo-biopolymer in bioreaetor culture was investigated. The maximum production of mycelia and exo-biopolymer was obtained at 350 rpm, where the maximum concentrations of mycelial growth and exo-biopolymer were 62 g/L and 23 g/L, respectively. The effect of agitation speed on the hyphal length and the number of tips was examined. The hyphal length and the number of tips of Cordyceps sinensis 16 in bioreaetor culture at 350 rpm were increased up to 660 ${\mu}m$ and 14/ml, respectively.

• Mycobiology
• /
• 제35권3호
• /
• pp.145-149
• /
• 2007

The Elfvingia applanata (EA), Hericium erinaceum (HE), Grifola frondosa (GF), Pholiota nameko (PN), Pleurotus eryngii (PE), Trametes suaveolens (TS), Fomes fomentarius (FF), and Inonotus obliquus (IO) could produce the endo- (EN) and exo-biopolymer (EX) in submerged culture. The highest anti-complementary activity of the EN was exhibited by PN (49.1%), followed by HE (38.6%), TS (37.0%), and FF (33.0%), whereas the high activity of the EX was found with GF (59.8%), followed by HE (36.3%), TS (30.8%), and IO (28.8%). The EN of P. nameko (EN-PN) and EX of G. frondosa (EX-GF) were found to contain 78.6% and 41.2% carbohydrates, while 21.4% and 58.8% protein, respectively. The sugar and amino acid compositions of EN-PN and EX-GF were also analyzed in detail.

• 한국균학회지
• /
• 제15권4호
• /
• pp.231-237
• /
• 1987

1. Cassava의 전분을 이용하여 검정곰팡이(Aspergillus niger)를 $30^{\circ}C$에서 4일 동안 진탕배양 하였을 때 배지 중 Cassava의 전분의 함량이 4%, 6%, 8% 및 10%일 때 96시간 배양된 각각의 균체량은 13.35mg/ml, 15.78mg/ml, 14.48mg/ml 및 9.93mg/ml이었다. 2. Cassava의 전분을 이용하여 검정곰팡이를 $30^{\circ}C$에서 4일 동안 진탕배양하여 얻어진 균체를 Lowry 방법에 의하여 그 균체단백을 측정한 바 배지 중의 Cassava의 전분의 함량이 4%, 6%, 8% 및 10%일 때 각각의 균체단백은 2.6mg/ml, 2.7mg/ml, 2.5mg/ml 및 1.7mg/ml이었으며 6% 함유구에서 균체단백의 양이 가장 많았다. 3. 배지 중의 Cassava의 전분량을 6%로 맞추고 질소원의 종류를 $NaNO_3,\;(NH_4)_2SO_4$ 및 Urea를 사용하고 질소량을 Czapeck-Dox 배지에 있는 $NaNO_3$의 질소량의 50%, 100%, 200%, 400% 및 $800^{\circ}C$되게 변화시키면서 진탕배양한 바 균체생산량에는 차이가 없었다.

• Journal of the Korean Wood Science and Technology
• /
• 제33권5호통권133호
• /
• pp.92-98
• /
• 2005

This study was performed to get the basic information concerned to the optimum culture condition of Inonotus obliquus. Several solid media, PDA, MEA and Czapek-Dox, and three liquid media were adopted for the in vitro cultivation. Some main features of the fungal morphological characteristics under cultivation conditions were observed and described. Preliminary results showed that appearance of the mycelial mat, hyphal size and substrate pigmentation differed according to the media. The PDA medium was the most favorable substrate for the growth on solid culture, followed by MEA and Czapek-Dox media. Concerned to the addition of amino acids, 5 amino acids, such as alanine, alginine, isoleucine, leucine and threonine, enhanced to the mycelial growth. Isoleucine was shown the best fungal growth. An important morphological hyphal structure for the fungus, the setae, was found in abundance and diverse its shape and size. In liquid culture, fresh potato broth was the best growth stimulant of the fungus, followed by Malt extract and potato broth. Addition of yeast extract to the liquid media had improved the biomass, but not laccase production.

• Applied Biological Chemistry
• /
• 제50권1호
• /
• pp.1-5
• /
• 2007

새송이버섯의 biomass를 위한 최적조건을 규명한 결과, 균사체 spot 배양을 위한 최적 조건은 PDA배지를 사용하여 24$^{\circ}C$에서 18일 배양 시 최적인 것으로 판단하였으며, 새송이버섯 균사체 Biomass를 위한 bioreactor의 배양 최적조건은 PDMP배지를 사용하여 pH 5.5, 18$^{\circ}C$에서 27일 배양시 reactor의 반응은 최적의 상태를 나타낼 수 있을 것이라 판단되었다. 최적조건에서 bioreactor를 이용한 새송이버섯 균사체의 인공적인 대량 배양이 가능하였으며, bioreactor에서 대량 인공 배양된 균사체를 이용한 새송이버섯 재배 시, 자실체가 형성되어 새송이버섯의 대량생산을 위한 biomass가 가능하였다.