• Title/Summary/Keyword: mycelial

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Screening of-Lyophyllum Decastes-Highly Productive Cultivable Strains

  • Wei, Shenglong
    • 한국균학회소식:학술대회논문집
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    • 2014.05a
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    • pp.47-47
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    • 2014
  • In order to explore mycelial growth and fruiting body formation of Lyophyllum decates on different media, ten cultivation media by using cottonseed hull, sawdust, corn cob etc. as main components were designed for seven strains. The results showed that the mycelial colour of all strains are mainly snow-white, and the formula of media using corn cob as main materials was better than that using cottonseed hull and sawdust for mycelial growth, but no fruiting body was formed. The cottonseed hull medium with a small amount of sawdust, plant leaves, humus or fermented material and wheat was beneficial for fruiting formation. The incubation period for fruiting formation of strain 3001 was 108 days and the highest yield was-214.80 g/bag. Fructification of the strains tasted occurs successively in order of 3001, 1035, 1004 and 1013. It was concluded that different medium composition had significant effect on the mycelial growth and fruiting body formation.

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Condition of Exo-polysacchride Production from Submerged Mycelial Culture of Ganoderma lucidum by Using Air-lift Fermenter System (Air-lift Fermenter System을 이용한 Ganoderma lucidum 균사체의 심부배양에 의한 세포외 다당류의 생산 조건)

  • 이신영;강태수;이만춘
    • KSBB Journal
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    • v.13 no.5
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    • pp.547-553
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    • 1998
  • For the efficient production of a new exo-polysaccharide from Ganoderma lucidum ASI 7004, the optimum conditions and methods in submerged cultivation were investigated with an airlift fermenter system. The optimum aeration rate was 2.5 Wm at the initial pH 5.0 and 28$^{\circ}C$. The increase of dissolved oxygen concentration by pure oxygen supply during cultivation did not improved the exo-polysaccaride production and the mycelial growth. The maximum exo-polysaccharide production and the mycelial growth under the optimum culture condition were obtained in media of glucose 60g/L, yeast extract 6g/L, (NH4)2HPO4 1g/L and KH2PO4 0.5g/L. Under these optimum medium and culture conditions, about 7.15g/L of exo-polysaccharide and 13.9g/L of mycelial growth were producted, respectively.

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Effect of Natural Oil on the Mycelial Growth of Flammulina velutipes (팽이버섯 균사체 생장에 미치는 Natural Oil의 영향)

  • Ahn, Jang-Hyuk;Lim, Wang-Jin;Song, Chi-Hyeun;Yang, Han-Chul
    • Microbiology and Biotechnology Letters
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    • v.21 no.4
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    • pp.306-309
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    • 1993
  • Vegetable oils supplemented to the basal medicum stimulated mycelial growth of Flammulina velutipes. The mycelial yield was increased 3.5 folds by addition of 3% (v/v) ricebran oil. Maximum mycelial yield (18.2mg/ml) was obtained by addition of 3.0% ricebran oil with 1.0% $CaCl_2$ to the basal medium. There was no significant difference between the liquid and solid spawn in the yield of sporophores.

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Biocontrol Effect of Gliocladium virens G1 and Soil Amendment on Astragal Stem Rot Caused by Rhizoctonia solani

  • Chung, Bong-Koo;Yun, Kyung-Ho
    • Mycobiology
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    • v.28 no.4
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    • pp.180-184
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    • 2000
  • In order to find an environment-friendly method to suppress astragal stem rot caused by the isolates of Rhizoctonia solani AG 1 and AG 4, we tested an antagonistic fungus Gliocladium virens G1 was evaluated as a biocontrol agent and estimated inorganic compounds and organic materials were tested for their effect of the disease suppression. G. virens G1 effectively inhibited mycelial growth in a dual culture and caused mycelial lysis in the culture filtrate. No adverse effect was observed when examined for seed germination and seedling growth. Promoted seedling growth was observed with the seed treatment. Seeds of astragal plant were germinated higher in the sterile soil than the natural soil. Of 14 inorganics tested, alum, aluminum sulfate and calcium oxide significantly suppressed the mycelial growth and sclerotial germination. Milled pine bark and oak sawdust also suppressed the mycelial growth. Soil amended with 1% of G. virens G1 composted with pine bark (w/v) significantly controlled astragal stem rot in the glasshouse experiments.

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Bottle Cultivation of Pleurotus ostreatus, Agrocybe aegerita and Ganoderma lucidum using Rice hull media

  • Lee, he-duck;Kim, hong-kyu;Kim, yong-gyun;Lee, ga-soon
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2001.04a
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    • pp.44-46
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    • 2001
  • Rice hull was used as a additive in order to find the effect for incresing of mushroom growth and yield in Chungnam Provincial techinical institution. 1 Treatment of 80% rice hull in small Neutaribeosut mycelial grow duration is shorter about 11 days and yield increased about 7% than conventional culture. 2. In case of Chongpung Neutaribeosut bottle culture, mycelial growth duration is shorter about two to three days in additive of 30 to 80% rice hull compared to conventional but yield similar to conventional. 3. Treatment of 30% rice hull in Agrocybe aegerita bottle culture, mycelial growth and yield increased 6days and 6% than convrntional, respectively 4 Treatment additived of 30% to 40% rice hull in Ganoderma lucidum bottle culture, similar to 454ays demand in mycelial grow duration and 38g yield/bottle in conventioal culture methods.

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Optimization of submerged culture conditions for the mycelial growth and exo-biopolymer production by Cordyceps millitaris

  • Park, Jong-Pil;Sinha, Jayanta;Song, Chi-Hyeon;Yun, Jong-Won
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.317-320
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    • 2000
  • The optimal temperature and pH for both mycelial growth and exe-biopolymer production by Cordyceps millitaris in shake flask culture were found to be $20^{\circ}C$ and 6.0, respectively. Sucrose (4%) and corn steep powder (1%) were the most suitable carbon and nitrogen source for mycelial growth and exo-biopolymer production. The maximum specific growth rate $(0.142h^{-1})$ was achieved when sucrose was used as the sole carbon source. Exo-biopolymer production was increased with the increase in C/N molar ratio concentration, probably due to the facilitated carbon uptake. Under the optimal culture conditions, the maximum mycelial growth exe-biopolymer concentration were reached to around 13.3 g dry cell weigh/l and 3.33 g/l, respectively.

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Production of Pleurotus spp. Mycelium Using Rancid Frying Oils (산패유를 이용한 느타리버섯 균사체의 생산)

  • 정기태;주인옥
    • KSBB Journal
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    • v.11 no.5
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    • pp.572-576
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    • 1996
  • Conditions for the culture of Pleurotus spp. mycelium using rancid frying oils were investigated. Among the six strains tested, Pleurotus ostreatus CBS 03 showed the greatest mycelial growth on fish paste and ramyon frying oil, and was used in this study. The optimum temperature and pH for mycelial growth were from 25 to $30^{\circ}C$ and pH 5.5 to 6.0, respectively. Tryptone for mycelial growth was better than any other nitrogen sources. The addition of $KH_2PO_4 and MgSO_4$ enhanced mycelial growth at 0.2 and 0.01% on fish frying oil, and at 0.1 and 0.03% on ramyon frying oil. Among the vitamins used, thiamine and nicotinic acid were the most effective ones.

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Cross- and Double-Resistance of Benomyl-Resistant Botryosphaeria dothidea (Benomyl에 저항성인 사과 겹무늬썩음병균의 교차 및 이중저항성)

  • 이창은;박석희
    • Korean Journal Plant Pathology
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    • v.10 no.4
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    • pp.270-276
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    • 1994
  • Mycelial growth resistant isolates of Botryosphaeria dothidea to benomyl showed 99~79% spore germination on the PSA media supplemented with 200~2,100 $\mu\textrm{g}$/ml of carbendazim and thiophanate-methyl to manifest the high cross-resistance in spore germination. Mycelial growth, 23~9 mm in colony diameter, also manifested the high cross-resistance of mycelial growth together with similarity of spore forming cross-resistance. Benomyl resistant isolates BR1, BR2 and BR3, grew 23~10 mm in colony diameter at 330~3,000 $\mu\textrm{g}$/ml of captafol, captan and oxine-copper showing the high double resistance of mycelial growth and spore formation with minor difference. However, within concentration range of the 3 fungicides tested, germinations of all the tested isolates were completely suppressed to show no double-resistance in the fungal spore germination.

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Morphological Changes of Hansenula anomala B-7 by Cadmium Ion (카드뮴이온에 의한 Hansenula anomala B-7의 형태 변이)

  • 송형익;유대식
    • Korean Journal of Microbiology
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    • v.29 no.6
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    • pp.397-401
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    • 1991
  • Yeast-form cells of cadmium ion-tolerant Hansenula anomala B-7 were changed to mycelial cells in medium containing more than $400\mu$g/ml of cadmium. Moreover, the mycelial cells were exchanged into clumped cells in a medium containing more than $1,000\mu$g/ml of cadmium. Optimal conditions of mycelial cell formation were achieved in the presence of .$1,000\mu$g/ml of cadmium with shaking cultivation for 7 days. Glucan and mannan contents of the yeast cell wall frown with $1,000\mu$g/ml of cadmium decreased by 10% compared with those grown without cadmium. However, protein and lipid contents increased about 20% respectively. By cadmium, no significant findings in specific amino acid contents were discovered.

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Effect of some reagents added in culture media upon the mycelial growth of the Psallitoa Campestris (Psalliota Campestris의 균사생장(菌絲生長)에 미치는 무기염류(無機鹽類)의 영향(影響))

  • Yu, Tae-Jong
    • Applied Biological Chemistry
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    • v.6
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    • pp.57-59
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    • 1965
  • According to the result of the observations on the effect of the reagents, $CuSO_4{\cdot}5H_2O$, $ZnSO_4{\cdot}7H_2O$ $MnSO_4{\cdot}4H_2O$, $HgCl_2$, upon the mycelial growth of psalliota campestris $HgCl_2$ showed the strongest effect on checking the mycelial growth $(,.008{\sim}0.01%)$ $CuSO_4{\cdot}5H_2O(0.030%)$, $ZnSO_4{\cdot}7H_2O(0.080%)$, came next and the $MnSO_4{\cdot}4H_2O(0.100%)$ was the weakest. Writer also found that showed the promoting effect of the mycelial growth in appropriate Concentration (at $0.015%\;CuSO_4{\cdot}5H_2O$, $0.030%ZnSO_4{\cdot}7H_2O$).

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