• Journal of Ginseng Research
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• 제41권4호
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• pp.608-614
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• 2017

Background: Ginsenoside Rg1 belongs to protopanaxatriol-type ginsenosides and has diverse pharmacological activities. In this report, we investigated whether Rg1 could upregulate muscular stem cell differentiation and muscle growth. Methods: C2C12 myoblasts, MyoD-transfected 10T1/2 embryonic fibroblasts, and HEK293T cells were treated with Rg1 and differentiated for 2 d, subjected to immunoblotting, immunocytochemistry, or immunoprecipitation. Results: Rg1 activated promyogenic kinases, p38MAPK (mitogen-activated protein kinase) and Akt signaling, that in turn promote the heterodimerization with MyoD and E proteins, resulting in enhancing myogenic differentiation. Through the activation of Akt/mammalian target of rapamycin pathway, Rg1 induced myotube growth and prevented dexamethasone-induced myotube atrophy. Furthermore, Rg1 increased MyoD-dependent myogenic conversion of fibroblast. Conclusion: Rg1 upregulates promyogenic kinases, especially Akt, resulting in improvement of myoblast differentiation and myotube growth.

• Physical Therapy Rehabilitation Science
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• 제4권1호
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• pp.38-43
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• 2015

Objective: To investigate and compare the size of the rectus femoris (RF), tibialis anterior (TA), and medial gastrocnemius (GMM) using ultrasound (US) imaging in young, elderly, and very elderly groups. Design: Cross sectional study. Methods: This study consisted of 25 young (age 20 years), 24 elderly (age 65-74 years), and 25 very elderly (age 75-90 years) people with no physical dysfunctions. The cross sectional area (CSAs) of the RF and muscle thickness of the TA and GMM were measured at rest and during contraction using an US system. Results: The CSA of the RF and thickness of the TA and GMM were significantly smaller in the elderly and very elderly groups than in the young group (p<0.05). There was a significant difference of the CSA of the RF at rest and during contraction between elderly and very elderly group (p<0.05). In the comparison of the TA and GMM thickness between elderly and very elderly groups, there were no significant differences except for the TA thickness during contraction. There was a significant difference in the percentage change in RF CSA among the three groups (p<0.05). Conclusions: Our results revealed loss of muscle mass in the RF, TA, and GMM in elderly and very elderly people (${\geq}65$ years old). In particular, the greatest age-related decline in muscle mass was observed for the RF. Furthermore, the CSA of the RF declined with aging in the very elderly groups (${\geq}75$ years old).

• 한국임상수의학회지
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• 제36권2호
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• pp.123-125
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• 2019

A 12-year-old castrated Toy Poodle was referred with a continuous non-weight bearing lameness of right hind limb due to a traffic accident 9 years ago. Physical examination and radiographs revealed partial loss of right calcaneal bone, loss of Achilles tendon and disused muscular atrophy. Arthrodesis was performed to preserve the function of the right hind limb. Pan tarsal arthrodesis plate was applied medially with 2.7 mm cortical screws and 2.0 mm cortical screws. During fixation, cancellous bone chip was transplanted into the arthrodesis site. A bone union was confirmed 9 weeks after surgery. Furthermore, on the gait analysis, the weight-bearing of right hind limb was restored to 70% of the opposite hind limb. Arthrodesis can be used to treat more difficult and serious problems affecting the joints. In particular, arthrodesis is indicated in cases such as comminuted intraarticular fractures and irreparable injury of the calcaneal tendon apparatus in the hock joint. In this case, the pan tarsal arthrodesis provided stability to the hock joint and improved the gait by restoring severe chronic damage. In conclusion, we successfully treated a challenging disability of hock joint using pan tarsal arthrodesis to restore the legs that were non-weight bearing due to chronic injury by traffic accident and objectively ascertained the increased weight bearing by gait analysis.

• 생명과학회지
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• 제31권5호
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• pp.453-463
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• 2021

청력 장애(hearing loss)는 임상적 및 유전적으로 상당히 이질적인 질병들의 그룹으로, 일반적으로 증후군 유형(syndromic type)과 비증후군 유형(non-syndromic type)으로 구분된다. 상염색체 열성의 청력 장애 환자가 다른 나라들에 비해 파키스탄에서는 상대적으로 흔하게 관찰되는데, 그 원인으로는 빈번한 근친 결혼의 문화가 일부 관여할 것으로 여겨진다. 본 연구는 상염색체 열성의 청력 장애 환자가 있는 두 파키스탄의 근친 가계를 대상으로 전장 엑솜 서열분석(whole exome sequencing)을 실시하여 유전적 원인을 규명하기 위해 수행되었다. 환자의 유전체 분석 결과, 우리는 언어 습득전 발병(prelingual onset)의 청력 장애 가족으로부터 MYO7A 유전자에서 병원성으로 판단되는 동형접합성 돌연변이인 p.Leu326Gln을 분리하였으며, 조기 발병의 청력 장애와 동시에 근위축(muscular atrophy)을 나타내는 환자 가족에서는 병원성이 확실하지 않는 두 변이(variants of uncertain significance)를 GPR98 유전자(p.Val3094Ile)와 PLA2G6 유전자 (p.Asp56Gly)에서 각각 분리하였다. MYO7A 및 PLA2G6 유전자의 missense 돌연변이는 고도로 보존된 단백질 부위에 위치했으며, 인실리코 분석(in silico analysis)에서도 병원성을 예측하였다. 그러나, GPR98 유전자의 돌연변이는 보존성이 다소 낮은 부위에 위치하였으며, 대부분의 인실리코 분석도 비병원성으로 예측했다. 동형접합성 매핑(homozygosity mapping)을 실시하였을 때, 각 가계에서 분리된 동형접합성 돌연변이의 두 대립유전자가 모두 단일 기원에서 유래한 것으로 예측되었는데, 이것은 근친 결혼에 기인한 것으로 판단된다. 본 연구는 파키스탄의 상염색체 열성 청력 장애 환자들의 정확한 분자진단 및 치료에 도움을 줄 수 있을 것으로 기대된다.

• Parasites, Hosts and Diseases
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• 제33권4호
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• pp.313-322
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• 1995

서을대학교 의과대학 기생충학교실 및 풍토병연구소1). 전남대학교 의과대학 병리학교실f) 요코가와흡충을 실험적으로 마우스(ICR)에 감염시키고 프레드니솔론을 주입하여 마우스의 면역 반응을 저하시킬 때 장의 병변과 충체 침입부위이 변화를 알아보기 위하여 이 연구를 시행하였다 ICR계 마우스를 비감염 대조군 요코가와흡충 감염군(마리당 피낭유충 1.800개 감염). 및 요코가와흡충 감염면역억제군으로 나누고 감염 후 6시간부터 3주까지 관찰하였다. 요코가와흡충 감염군에 있어서는 감염 1일까지 많은 충체가 장 절편내에서 발견되었고. 장 병변은 점막에 국한되어 있었다. 그러나 감염 3일 이후부터는 충체가 거의 발견되지 않았고. 1주 후에는 이미 장 병변이 회복되어 가는 양상을 보였다 한편 면역억제 따우스에 있어서는 감염군과는 달리 장 절편내에서 전기 간동안 충체가 발견되었고 감염이 오랫동안 유지되었다 염증 반응은 전 기간을 통해 미미하였고. 감염 3일까지 장 병변도 융모의 부종이나 상피층의 가벼운 위축 외에는 거의 관찰되지 알았다. 그러나 감염 5일째에는 마우스 십이지장 및 공장의 응모 및 장선이 매우 심하게 파괴되고 장벽으로부터 탈락되는 양상을 보였으며 이때 충체는 점막하 조직을 통과하고 거의 근육층까지 침입하여 장벽을 압박하고 있었다. 이러한 소견은 계속된 면역억제에도 불구하고 감염 7일부터 21일까지 점차 회복되는 양상을 보였다. 이상의 결과는 요코가와흡충을 면역억제 마우스에 감염시키면 짧은 가간 동안 장 점막의 심한 파괴가 일어나고 점막하층까지 충체가 침입할 수 있음을 나타내었다.

• 대한간호학회지
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• 제25권2호
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• pp.193-209
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• 1995

The purpose of this study was to determine the effect of intermittent low - intensity, short duration exercise during hindlimb suspension on the mass, relative weight, myofibrillar protein content, cross-sectional area of Type I and Type II fibers and SDH activity in Type II(plantaris) muscle. To examine the effectiveness of intermittent low-intensity, short duration exercise on mass, myofibrillar protein content and fiber size, the hindlimbs of adult female Wistar rats were suspended(HS) and half of these rats walked on a treadmill for 45 min/day(9 min every 2h) at 5m /min and a 15$^{\circ}$grade (HS-EX). Plantaris wet weight was 19.67% significantly smaller(p<0.005) and relative plantaris weight was 6.25% smaller compared with those of control rats following seven days of hindlimb suspension. Plantaris wet weight and relative plantaris weight increased by 27.66%, 12.22% each through intermit-tent exercise during hindlimb suspension(p<0.005, p<0.05), moreover, plantaris wet weight and relative plantaris weight of the HS-EX rats were similar to those of control rats. Soleus wet weight and relative soleus weight decreased significantly by 31% and 22.0% in the HS rats(p<0.05). Soleus wet weight and relative soleus weight increased by 10.41%, 25.64% respectively through intermittent ex-ercise during hindlimb suspension, furthermore, soleus wet weight and relative weight of the HS-EX rats were closer to those of control rats. Myofibrillar protein content of plantaris and soleus decreased significantly by 51.49%, 59.65% each, following seven days of hindlimb suspension (p<0.005) Myofibrillar protein content of plantaris and soleus increased by 51.79%, 75.47% each with significance through intermittent exercise during hindlimb suspension(p<0.005). Myofibrillar protein content of plantaris and soleus in HS-EX rats was smaller than that of control rats. No change was observed in fiber type percentage following 1 week of hindlimb suspension or exercise during hindlimb suspension. The type I fiber cross-sectional area of both soleus and plantaris muscle was 18.72% and 41.07% lower in the HS than that of the controls (p<0.05, p<.001), that of both muscles was 6.60% and 29. 3％ greater in the HS-EX than that of the HS rats. HS plus intermittent low- intensity short duration exercise resulted in Type I fiber cross-sectional area closer to the controls. Type II fiber cross-sectional area of both plantaris and soleus muscle was 22.45% and 22.58% sl nailer in the HS than in the controls, that of both muschles in the HS-EX was 14.10%, 5.78% greater than HS. Intermittent exercise during hindlimb suspension resulted in Type I, II fiber cross-sectional area closer to the control value. There was no change in SDH activity following 1week of hindlimb suspension or exercise during hindlimb suspension in the plantaris muscle. The results suggest that intermittent low intensity short duration exercise can ameliorate Type II muscular atrophy Induced by hindlimb suspension.

• Journal of Nutrition and Health
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• 제12권4호
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• pp.29-42
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• 1979

In order to observe metabolic effects of an oral conceptive, Norinyle, on female Guinea pig, the changes of ascorbic acid amount and alkaline phosphatase activity in the liver and serum were determined, and histochemical changes of the uterus were observed by microscopic and electronmicroscopic methods by administration of Norinyle with or without ascorbic acid. The results obtained are summerized as follows: 1) The metabolic changes were clearly influenced by the administration of Norinyle alone, but the changes were diminshed by administration of Norinyle with ascorbic acid. 2) The adimnistration of Norinyle influenced to increases the requirment of ascorbic acid in the liver. 3) The uterus weight of the Norinyle administered group was much increased, while the weight was less increased in the group of administered Norinyle with ascorbic acid than the control. 4) The Norinyle administration was brought about an atrophy of endometrium, of uterus especially, functional layer, that the formation of glands were inadequately and the fromation of basal layer and stroma were diminished. 5) An acute infarction on the all layers of the uterus was developed at 9th and 25th days of Norinyle administration and 20th day of Norinyle with ascorbic acid administration. 6) A hypertrophy of stromal and endovascular cells were observed on the groups administered of Norinyle alone(group II ) or Norinyle with ascorbic acid(group IV). 7) It was observed that amount of collagen fiber in the basal and muscular layeres of uterus were diminished under a microscopical observation by the special stained specimen on the Norinyle administered group, but the amount and distribution of reticulin fiber were not changed significantly. 8) The fille structure of outer functional layer of the uterus were significantly changed by administration of Norinyle which were shown irregurarity of nuclear membrane, poor development ana significant expansion of enaoplasmic reticulum, decreases of the amount of ribosome due to slip off, increases of the number of dense bodies, obvious formation of vaccule, an4 decreases the amount of collagen in inner and outer layer of the stroma. 9) The amount of ascorbic acid in the serum did not much changed but the amount in the liver was much decreased by the administration of Norinyle, And the administration of Norinyle with ascorbic acid induced for a significant diminishing on the changes of uterus which might be able to developed by the administration of Norinyle alone.

• Archives of Reconstructive Microsurgery
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• 제6권1호
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• pp.73-79
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• 1997

Complete denervation after severe brachial plexus injury make significant muscle atrophy with loss of proper function. It is much helpful to reconstruct the essential function of the elbow flexion movement in patient with total loss of elbow flexion motion after brachial plexus lesion which was not recovered with nerve surgery or long term conservative treatment from onset. In whole arm type brachial plexus injury, if there were no response to neurotization or neglected from injury, the volume of the denervated muscle is significantely reduced month by month. About 18 months most of the muscle fibers change to fibrous tissues and markedly atrophied irreversibly, further waiting is no more meaningful from that period. Authors performed 14 cases of functioning gracilis muscle transfer from 1981 to 1995 with microneurovascular technique, neuromusculocutaneous free flaps were performed for reconstruction of lost elbow flexion function. Average follow-up period was 5 years and 6 months. We used couple of intercostal nerves as a recipient nerve which were anastomosed to muscular nerve from obturator nerve in all cases. Recipient vessels were three deep brachial artery and eleven brachial artery which were anastomosed to medial femoral circumflex artery with end to end or end to side fashion. Average resting length of the transplanted gracilis were 24 cm. We can get average 54 degree flexion range of elbow with fair muscle power from flail elbow. There were one case of muscle necrosis with lately developed thrombosis of microvascular anastomosed site which comes from insufficient recipient arterial condition, 3 cases of partial marginal necrosis of distal skin of the transplanted part which were not significant problem with spontaneously solved with time goes by gracilis muscle has constant neurovascular pattern with relatively easy harvesting donor with minimal donor morbidity. Especially it has similar length and shape with biceps brachii muscle of upper arm and longer nerve pedicle which can neurorrhaphy with intercostal nerve without nerve graft if sufficient mobilization of the nerves from both sides of gracilis and intercostal region. Authors can propose gracilis muscle transplantation with intercostal nerves neurotization is helpful method with minimal donor morbidity for neglected brachial plexus palsy patients.

• 한국임상수의학회지
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• 제23권2호
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• pp.190-193
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• 2006

개에서 방사선 검사로 확진한 둔부 골관절염 증례에 대하여 hyaluronic acid의 관절강내 주사 및 caprofen의 경구투여를 2주간 치료를 하였으나, 임상증상이 개선되지 않았다. 봉독 수침요법(5일 간격, 7회)과 압통점 치료를 병용하였다. 수침에 사용된 혈위는 이환 된 우측 환지의 5개 혈위를 사용하였다: GB30(국소혈), ST35, GB33, BL40 및 LIV08(원위혈). 수침재료(0.2 ml/혈위, 전체 1 ml/회)는 apitoxin $100{\mu}g+2%$ 리도카인 (1:1)+생리식염수로 제조하였고, 5개 혈위에 각각 0.2 ml씩 수침하였다. 또한 우측 환지의 대퇴사두근의 압통점에 2% 리도카인(0.2ml/압통점)을 매회 치료 시 각각 수침하였다. 봉침 수침 요법은 임상증상을 급속하게 개선시켰는데, 파행 및 보행실조가 7회 치료(30일) 후 소실되었으며, 우측 후지의 근육 위축이 많이 호전되었고, 치료 종료 2주 후(44일)에 방사선 검사 상 환부는 거의 정상소견을 나타내었다. 본 환축은 봉독 수침 요법과 압통점 치료의 병용으로 양호한 치료반응을 나타난 개의 둔부 골관절염의 증례이었다.

• Archives of Plastic Surgery
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• 제37권6호
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• pp.783-787
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• 2010

Purpose: There are many articles describing about Guyon canal compression syndrome. Until recently, most of these articles have been presented about the symptoms of ulnar nerve compression, but there have been no reports about ulnar artery compression. In this article, besides the nerve compression symptoms in the Guyon's canal, we represented the symptoms and treatments based on the ulnar artery obstruction. Methods: Guyon canal is composed of the hamate and pisiform, and the ligaments which connect them. The course of the ulnar nerve and artery, which passes through this narrow canal, is affected by the anatomical structure of the base of the canal. Out of 14 patients (21 cases) were retrospectively reviewed in this study from 2006 to 2009. Of 14 patients, there were 5 men and 9 women with ages between 21 to 61 years old. The symptoms had volar sensory loss of ulnar sided digits, with muscular atrophy of hypothenar muscles. Prior to surgery, most of these patients had vascular disorders which was diagnosed definitively by angiography and electromyogram. Results: The release of Guyon canal and interposition graft of the obstructed arteries was carried out to 11 patients (15 cases) who had artery (vascular) occlusive disorder, and. 12 cases had sympathectomy and interposition graft after resection of obstructed ulnar artery. Six cases had release of carpal tunnel performed simultaneously. There were no major complications after surgery. The circulation of the ulnar artery was improved along with the patients' symptoms. Conclusion: The pre-existing articles about Guyon canal compression syndrome were mainly focused on ulnar nerve compression. This study, which was carried out by our department, showed that most of these patients had ulnar artery obstruction or stenosis simultaneously with ulnar nerve compression. The vascular disorder was corrected by interposition graft after the resection of the site of ulnar artery occlusion. And to conclude, When we resolve the ulnar nerve compression, the proper diagnosis & treatment of impaired ulnar artery circulation should be carried out concomitantly.