• Asian-Australasian Journal of Animal Sciences
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• 제20권5호
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• pp.622-626
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• 2007

Using multi-trait animal model BLUP, selection was conducted over seven generations for growth rate (DG), real-time ultrasound loin-eye muscle area (LEA), backfat thickness (BF), and intramuscular fat content (IMF) to develop a new line of purebred Duroc pigs with enhanced meat production and meat quality. This study was intended to investigate the relationship between restriction fragment length polymorphism (RFLP) of a heart fatty acid-binding protein (H-FABP) gene and intramuscular fat content (IMF) of this Duroc purebred population. The present experiment examined the RFLP of 499 slaughtered pigs. The DNA was separated from the blood or ear tissue of the pigs, which were slaughtered at 105 kg of body weight. Intramuscular fat content of the longissimus muscle was measured using chemical analysis. A significant difference was detected in the breeding value of IMF among the H-FABP PCR RFLP genotypes. The AA genotype has a significantly larger positive effect on the IMF breeding value than do the Aa and aa genotypes for the MspI RFLP. In addition, the DD genotype has a significantly greater positive effect on IMF breeding value than the Dd and dd genotypes for the HaeIII RFLP. For the HinfI RFLP, the hh genotype has a significantly larger positive effect on IMF breeding value than the HH genotype. Multiple regression analysis was performed using the IMF breeding values as the dependent variable and the three H-FABP genotypes as independent variables. Results revealed that the contribution of the genotypes to variation in IMF breeding values was approximately 40%. These results demonstrated that H-FABP RFLPs affect IMF in this Duroc population.

• Asian-Australasian Journal of Animal Sciences
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• 제20권5호
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• pp.768-774
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• 2007

To study the effect of dietary docosahexaenoic acid (DHA) enrichment on the expression of hepatic genes in pigs, weaned, crossbred pigs (30 d old) were fed diets supplemented with either 2% tallow or DHA oil for 18 d. Hepatic mRNA was extracted. Suppression subtractive hybridization was used to explore the hepatic genes that were specifically regulated by dietary DHA enrichment. After subtraction, we observed 288 cDNA fragments differentially expressed in livers from pigs fed either 2% DHA oil or 2% tallow for 18 d. After differential screening, 7 genes were found to be differentially expressed. Serum amyloid A protein 2 (SAA2) was further investigated because of its role in lipid metabolism. Northern analysis indicated that hepatic SAA2 was upregulated by dietary DHA enrichment (p<0.05). In a second experiment, feeding 10% DHA oil for 2d significantly increased the expression of SAA2 (compared to the 10% tallow group; p<0.05). The porcine SAA2 full length cDNA sequence was cloned and the sequence was compared to the human and mouse sequences. The homology of the SAA2 amino acid sequence between pig and human was 73% and between pig and mouse was 62%. There was a considerable difference in SAA2 sequences among these species. Of particular note was a deletion of 8 amino acids, in the pig compared to the human. This fragment is a specific characteristic for the SAA subtype that involved in acute inflammation reaction. Similar to human and mouse, porcine SAA2 was highly expressed in the liver of pigs. It was not detectable in the skeletal muscle, heart muscle, spleen, kidney, lung, and adipose tissue. These data suggest that SAA2 may be involved in mediation of the function of dietary DHA in the liver of the pig, however, the mechanism is not yet clear.

• 한국응용과학기술학회지
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• 제21권2호
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• pp.182-189
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• 2004

On contraction of the muscles, marked changes in X-ray reflections are observed, suggesting that conformational changes of contractile molecules and the movement of myosin heads during muscle contraction. Time slice requires tension peak after the onset of stimulation and the height of tension peak depends on the number of twitch cycle. The muscles were stimulated by five successive stimuli at an interval of 80 ms started while the tension was still being exerted by the muscles. The intensity of $I_{11}$, $I_{10}$, $143{\AA}$ and $215{\AA}$ reflection measured with 5ms time resolution and is recorded in isometric tension. The peak height of $I_{11}$ and $143{\AA}$ intensity is changed after the onset of a stimulation $I_i$, and the length of twitch is shortened by successive twitches in the case of stimulation $T_i$. On the other hand, the peak height of In and $215{\AA}$ intensity starts to decrease at the 1st twitch and remains constant at low peak height without appreciable recovery during the contraction term. In the case of successive twitch stimulation, the myosin heads of muscle are once moved from their resting position and never returned to their initial position.

• 한국수산과학회지
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• 제49권1호
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• pp.7-12
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• 2016

Total mercury (TM) is a hazardous element that is of particular concern to human health. Due to the diversity of dietary habits among fishes, tissue-specific analysis of hazardous elements is necessary. In this study, the tissue-specific TM in cultured fish was analyzed to conduct risk assessment. The highest concentrations of TM were found in the farmed marine fish Pagrus major (0.111 mg/kg) and in the farmed freshwater fish Channa argus (0.162 mg/kg). TM concentration was significantly correlated with total fish length (P<0.01). Significant differences in TM were found between three types of fish tissue, with the concentration in fish muscle being significantly higher than those of gill or liver (P<0.01). Moreover, the tissue-specific TM concentrations of farmed freshwater fish were significantly higher than those of farmed marine fish (P<0.01). According to the risk assessment, the TM body exposure rate of muscle and liver in cultured fishes ranged from 0.001 to 0.389% of the Provisional Tolerable Weekly Intake. Therefore, these results showing the tissue-specific TM contents of cultured fish could be useful to assess the health risks of Korean dietary habits.

• The Journal of Korean Physical Therapy
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• 제21권1호
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• pp.73-80
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• 2009

Purpose: In order to examine difference in the activity of potential of spinal neurons and cortical neurons according to muscle contraction type in post stroke hemiplegic subjects and healthy subjects, the present study conducted an experiment as follows. Methods: The subjects in the experimental group were 17 left-side hemiplegic subjects (9 female, 8 male; mean age, 63.41$\pm$9.86 years) with the right hand as the dominant hand selected among post stroke hemiplegic subjects, and 17 age matched healthy control subjects (10 female, 7 male; mean age, 64.12$\pm$12.07 years). Movement-related cortical potentials (MRCPs) were measured using surface electromyography and electroencephalography while concentric and eccentric movements were made alternately. Results: As to the pattern of the activity of MRCP, which indicates the activity of motor cortical neurons, we found that the amplitude is high (p<0.01), the length of excitement is short (p<0.01) and the ascending gradient of amplitude to the peak increases (p<0.05) in post stroke hemiplegic subjects' lesion sites different from healthy subjects. Conclusion: The activity of cortical neurons was no difference in activity according to contraction type was observed in post stroke hemiplegic subjects' lesion sites. This suggests that there is no distinction in the activity of cortical neurons between concentric contraction and eccentric contraction. Accordingly, if post stroke hemiplegic subjects' activity of motor cortex is analyzed by measuring MRCPs, it is considered useful in research on neural plasticity or as a ground of clinical effects in the area of physical therapy for the central nervous system.

• Asian-Australasian Journal of Animal Sciences
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• 제19권7호
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• pp.931-937
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• 2006

The full-length cDNA of the porcine SMPX gene was obtained by the rapid amplification of cDNA ends (RACE). The nucleotide sequences and the predicted protein sequences share high sequence identity with both human and mouse. The promoter of SMPX was sequenced and then analyzed to find the promoter binding sites. The reverse transcriptase-polymerase chain reaction (RT-PCR) revealed that SMPX has a high level of expression in heart and skeletal muscle, a very low expression in lung and spleen and no expression in liver, kidney, fat and brain. Moreover, SMPX has a differential expression level in skeletal muscle, the expression in 65-day embryos being higher than other stages. The porcine SMPX was mapped to SSCXp24 by using a somatic cell hybrid panel (SCHP) and was found closely linked to SW1903 using the radiation hybrid panel IMpRH. An A/G single nucleotide polymorphism (PCR-RFLP) in the 3'-untranslated region (3'-UTR) was detected in eight breeds. The analysis of allele frequency distribution showed that introduced pig breeds (Duroc and Large White) have a higher frequency of allele A while in the Chinese indigenous pig breeds (Qingping pig, Lantang pig, YushanBlack pig, Large Black-White pig, Small Meishan) have a higher frequencies of allele G. The association analysis using an experimental population (188 pigs), which included two cross-bred groups and three pure-blood groups, suggested that the SNP genotype was associated with intramuscular fat content.

• 한국수산과학회지
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• 제25권6호
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• pp.474-484
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• 1992

황해서식 참조기(Pseudosciaena polyactis)에 대한 계군을 분석하기 위한 연구의 일환으로 우선 황해의 목포 연근해에서 채집된 참조기의 난자와 근육으로부터 mt-DNA와 근육 actin을 추출하였다. 난자의 경우 mt-DNA를 추출한 뒤 제한효소로 처리하여 절편다형현상을 관찰하였으며 근육 actin의 경우 단백질 분해효소(Staphylococcus aureus $V_8$ protease)로 처리하여 N-terminal 절편의 다형현상을 각각 관찰하였다. Mt-DNA의 genome 크기는 약 $\16\pm0.2$ Kb였고 전체 절편수는 37개였으며 사용된 20개의 제한효소 중 8개의 제한효소만이 3개 정도의 절편을 보이므로써 유의성을 관찰할 수 있었다 한편 근육 actin을 Staphylococcus aureus $V_8$단백질 분해효소로 처리하였을 때 N-terminal 부위에서 26 KDa 및 16 KDa의 분자량을 갖는 특이절편을 관찰할 수 있었다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제14권4호
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• pp.327-339
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• 1992

This study was undertaken to investigate the effect of orthognathic surgery on occlusal force. The maximum bite force was measured in 26 dentofacial deformity patients, aged 14-26(mean age 20.3) years, before surgery and at IMF removal, 3, 6, and 12months postsurgery. To grope the correlation of bite force and skeletal change after orthognathic surgery, the cephalometric headplates were measured, tabulated and statistically analyzed. The results were as follows. 1. The presurgical maximum bite force was 13.7kg in upper first molar(rt. Side 12.7kg, it. Side 14.6kg). There was remarkable difference with that of normal occlusion. 2. The recovery of bite force was very significant in according to the operation method and the duration of IMF that was 7.6kg at IMF removal, 14.2kg at 3 months, 19.7kg at 6 months. 26.1kg at 12 months postsurgery. 3. To fasten the recovery and to increase the bite force after orthognathic surgery, the long IMF time and the injury to the masticatory muscle should be avoided by the internal rigid fixation and early physical exercise. 4. The bite force was positively correlated to the changes of mandibular plane angle, the angle between platatal plane and mandibular plan, the angle between occlusal plane and mandibular plane, and negatively correlated to the changes of mandibular body length in craniofacial structure. 5. There was no correlationship between bit force and mesial inclination of tooth long axis of first molar in this subject. 6. There was no correlation between the changes of bite force and the changes of mechanical advantage of the temporal and masseter muscle.

• Radiation Oncology Journal
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• 제8권1호
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• pp.133-136
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• 1990

자궁암치료에서 재발되거나 치료가 어려운 종양에 대하여 방사선과 온열요법을 병행함으로서 치료 성과를 다소 향상시킬 수 있었다. 더욱이 방사선 근접조사와 강내온열치료는 주위 건강조직의 피해를 줄이면서 종양에 집중손상을 줄 수 있었으며 강내방사선조사 기구를 공동으로 이용하므로서 시술이 간단하고 치료부위를 정확히 조준할 수 있었다. 그러나 강내조사용 안테나는 그 모양과 구성에 따라 온열 분포가 변하며 재래식 쌍극철심형 안테나는 끝부분 또는 연결부위의 가온이 급증하여 균등한 온열분포를 기대할 수 없었다. 저자들은 안테나의 길이를 마이크로파의 약수 즉 3, 6, 12 cm로 하여 공명이 잘 이루어지도록 하였으며 끝이 굵고 접촉 부위가 가느다란 꼬깔형 (conical) 안테나를 제작하여 사용한 결과 안테나 축에 따라 거의 일정하거나 약간 타원형의 온도분포를 이루었으며 가온 깊이도 $2\~3\;cm$로서 비교적 깊은 곳까지 가열할 수 있어 강내 치료효과를 향상시킬 수 있다고 생각된다.

• Toxicological Research
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• 제33권2호
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• pp.157-164
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• 2017

This study was to evaluate the age-dependent effects of caffeine exposure on the long bones and reproductive organs using male rats. A total of 15 immature male rats and 15 young adult male rats were allocated randomly to three groups: a control group and two groups fed caffeine with 120 and 180 mg/kg/day for 4 weeks. Exposure to caffeine at either dose significantly reduced body weight gain; a proportional reduction in muscle and fat mass in immature animals, whereas a selective reduction in fat mass with relatively preserved muscle mass in young adult animals. The long bones of immature rats exposed to caffeine were significantly shorter and lighter than those of control animals along with decreased bone minerals. However, there was no difference in the length or weight of the long bones in young adult rats exposed to caffeine. Exposure to caffeine reduced the size and absolute weight of the testes significantly in immature animals in comparison to control animals, but not in young adult animals exposed to caffeine. In contrast, the adrenal glands were significantly heavier in caffeine-fed young adult rats in comparison to control animals, but not in caffeine-fed immature rats. Our results clearly show that the negative effects of caffeine on the long bones and testes in rats are different according to the age of the rat at the time of exposure, and might therefore be caused by changes to organ sensitivity and metabolic rate at different developmental stages. Although the long bones and testes are more susceptible to caffeine during puberty, caffeine has negative effects on body fat, bone minerals and the adrenal glands when exposure occurs during young adulthood. There is a need, therefore, to educate the public the potential dangers of caffeine consumption during puberty and young adulthood.