• 생명과학회지
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• 제26권4호
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• pp.387-397
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• 2016

본 연구는 17-DMAG이 골격근에서 autophagy에 관여하는 가를 조사하기 위해, C2C12세포와 마우스 골격근에서 17-DMAG (Hsp90 억제제/Hsp72 활성제)을 처치하는 그룹과 autophagy 억제제(Bafilomycin 또는 colchicine)를 처치하는 그룹과 처치하지 않는 그룹을 동시에 두고 autophagy flux를 측정하였다. C2C12 배양세포에서 17-DMAG이 Hsp90 억제/hsp72 활성화시켰으며 Akt-mTOR 신호체계를 유의하게 감소시켰지만(p<0.05) autophagy marker 단백질인 LC3 II와 p62를 증가시키지 않았다. in vivo 모델의 경우 17-DMAG 처치가 배양세포에서 발견된 것처럼 Hsp90억제/hsp72를 활성화시켰고 Akt-mTOR 신호체계를 유의하게 감소시켰다(p<0.05). 반면 LC3 II와 p62 단백질 수준은 autophagy 억제제(colchicine) 처치 수준보다 더 높게 증가되었다. 이는 17-DMAG이 골격근에서 autophagy를 증가시키지만 C2C12 배양세포에서는 autophagy의 활성화가 제한적임을 암시한다. 현재 이러한 in vitro와 in vivo 모델에서의 차이는 불분명하다.

• 생명과학회지
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• 제22권12호
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• pp.1651-1657
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• 2012

본 연구에서는 C2C12 근육 세포에서 IGF-I이 세포골격 연결 단백질인 plectin과 MACF1 유전자 발현에 미치는 영향에 대해 알아보았다. 그 결과 IGF-I이 plectin 유전자의 단백질과 mRNA 발현을 증가시켰으며, MACF1 mRNA 발현을 증가시켰음을 알 수 있었다. 이는 운동에 의해 근육에서 분비가 증가하는 IGF-I이 근육 관련 유전자들의 발현을 조절하여 근부피 유지에 영향을 미친다는 기존의 연구 결과들에서 더 나아가 골격근 구조 안정화 및 근수축 기전에 기여하는 plectin과 MACF1 유전자 발현에도 영향을 미친다는 사실을 증명하였다는데 의의가 있다고 사료된다. 향후 근수축 기전에 있어, 운동 형태, 근섬유의 종류에 따른 세포 골격 단백질의 역할 규명 및 조절자에 관한 연구가 더 수행된다면 운동이 골격근의 생리적 변화에 미치는 영향에 대한 추가적 정보를 제공할 수 있을 것이다.

• 대한물리의학회지
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• 제13권3호
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• pp.141-150
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• 2018

PURPOSE: This study was conducted to understand the adaptive responses of different modes of physical exercises utilizing skeletal muscle and the comprehensive relevance of AMPK signaling that can be activated by physical exercise as a potential molecular target in human health problems such as neuromuscular disorders (NMDs). METHODS: Most of the contents in this review article are based on recent publications concerning the main topics of interest. The reference literatures cited were obtained by basic searches of overseas academic databases such as PubMed and ScienceDirect using EndNote X7.8. RESULTS: The phenotypic adaptive responses of skeletal muscle during endurance- and resistance-based exercise training (ET and RT respectively) appear to be distinct. To explain the adaptive responses in each single mode of exercises (ET, RT) along with combined exercise training (CT), AMPK signaling is proposed as an important molecular link among those differential modes of exercise and a promising molecular target of NMDs. CONCLUSION: Based on the available evidence, intracellular AMPK signaling activated by diverse stimuli including physical exercise can be a potential and promising therapeutic target for the prevention, amelioration or cure of various human health problems including NMDs and may also be beneficial for physical rehabilitation and emergency situations that may elicit acute metabolic stresses.

• 생명과학회지
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• 제15권1호
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• pp.100-105
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• 2005

젖산탈수소효소(EC 1.1.1.27, lactate dehydrogenase, LDH) inhibitor는 햄스터와 소 골격근 미토콘드리아에서 분리하였다. 햄스터 골격근조직의 LDH inhibitor는 175 mM NaCl과 초음파로 분리하였다. 소 골격근조직의 미토콘드리아에서 분리된 inhibitor는 열에 강한 특성을 보였고, $A_4$ 동위효소에 대한 저해정도가 높았으며, 분자량은 22,000 kDa으로 나타났다. Inhibitor는 심장조직을 제외한 골격근, 신장 및 간 조직의 미토콘드리아에서 LDH 결합 시 중요하게 관여하고 있었다.

• 대한수의학회지
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• 제37권3호
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• pp.607-612
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• 1997

A case of neosporosis is described in a 6-month-old aborted bovine fetus. Grossly, numerous, well-demarcated, 2-3mm, yellowish white foci were scattered in the heart and skeletal muscle. Histopathologic changes were confined to the brain, heart, and skeletal muscle. Multifocal areas of necrotizing encephalitis were observed in the cerebrum as well as cerebellum. Severe multifocal to coalescing nonsuppurative inflammation was observed in the myocardium and skeletal muscle. The tachyzoites of Neospora caninum were demonstrated in the brain, myocardium and skeletal muscle with immunohistochemical method using Neospora caninum-specific antibody. Based on the histopathology and immunohistochemistry, this case was diagnosed as neosporosis in aborted Korean cattle. This is believed to be the first reported case of bovine neosporosis in Korea.

• Investigative Magnetic Resonance Imaging
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• 제13권1호
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• pp.101-105
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• 2009

저자들은 육종의 일종으로 분류되는 유방의 악성 엽상종양이 골격근으로 전이된, 현재까지 영상소견으로는 보고된 사례가 없는, 드문 증례의 초음파, 양전자방출 단층촬영, 전산화 단층 촬영, 자기공명영상 소견을 보고하고자 한다.

• 대한물리의학회지
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• 제18권4호
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• pp.67-75
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• 2023

PURPOSE: This study aimed to identify the clinical factors that contribute to age-related loss of skeletal muscle mass (ALSMM) among young Korean male adults. METHODS: This was a cross-sectional study involving 955 men aged between 20-29 years. They underwent screening to assess the ALSMM. The study examined a variety of factors, including age, height, weight, body mass index (BMI), waist circumference (WC), skeletal muscle mass index (SMI), lifestyle-related habits such as smoking and drinking status, systolic and diastolic blood pressure (SBP/DBP), fasting blood glucose (FBG) levels, as well as the serum triglyceride and total cholesterol (TC) levels. RESULTS: The variables that displayed significant associations with ALSMM were height, weight, BMI, WC, SMI, FBG, TC, DBP, and alcohol consumption (p < .05). Serum triglyceride levels, SBP, and smoking status did not exhibit statistical significance (p > .05). CONCLUSION: The study identified the contributing factors associated with the ALSMM in community-dwelling young adult males. These findings would enrich the current body of literature on ALSMM and provide potential risk factors associated with its development in young Korean males.

• 대한물리의학회지
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• 제19권1호
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• pp.43-51
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• 2024

PURPOSE: Sarcopenic obesity (SO) is a clinical condition that combines sarcopenia and obesity. This study examined the frequency of SO in young Korean females between 20 and 29 years of age. METHODS: The study involved 1,000 participants. The height, weight, body mass index (BMI), waist circumference, skeletal muscle mass index, fasting glucose, triglyceride, total cholesterol levels, systolic and diastolic blood pressure, alcohol consumption, and smoking status were the research variables. The skeletal muscle mass index was calculated as appendicular skeletal muscle mass (ASM) divided by the BMI. The ASM was assessed using dual X-ray absorptiometry. Complex sampling analysis and multiple logistic regression were used for analysis. RESULTS: A .74(.30-1.80) frequency of SO was observed. The statistically significant risk factors in females were height, weight, BMI, waist circumference, skeletal muscle mass index, total cholesterol, systolic blood pressure, and diastolic blood pressure (p < .05). CONCLUSION: Young Korean adults with SO have a .74(.30-1.80) frequency of occurrence that is linked to specific risk factors. Hence, primary care clinicians and health care professionals should consider these factors when patients require a referral for early detection and treatment. Healthcare professionals and clinicians can identify potential SO patients by acknowledging these risk factors.

• 한국정밀공학회지
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• 제24권11호
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• pp.116-125
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• 2007

Human muscle skeletal model was developed for biomechanical study. The human model was consists with 19 bone-skeleton and 122 muscles. Muscle number of upper limb, trunk and lower limb part are 28, 60, 34 respectively. Bone was modeled with 3D beam element and muscle was modeled with spar element. For upper limb muscle modelling, rectus abdominis, trapezius, deltoideus, biceps brachii, triceps brachii muscle and other main muscles were considered. Lower limb muscle was modeled with gastrocenemius, gluteus maximus, gluteus medius and related muscles. The biomechanical stress and strain analysis of human was conducted by proposed finite element analysis model under Kumdo head hitting motion. In this study structural analysis has been performed in order to investigate the human body impact by Kumdo head hitting motion. As the results, the analytical displacement, stress and strain of human body are presented.

• Archives of Reconstructive Microsurgery
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• 제12권1호
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• pp.1-12
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• 2003

The bridging of nerve gaps is still one of the major problems in peripheral nerve surgery. To evaluate the role of silicon tube in nerve regeneration, gaps were made by resection of tibial components of sciatic nerves of twenty-five New Zealand rabbits. The gaps were divided into five groups. In group I, the tibial components of sciatic nerves were isolated and the incision immediately closed. In group II, 1-cm segments of the nerve were removed and the silicon tubes filled with autogenous skeletal muscle were sutured in place. In group III, 1-cm segments of the nerve were removed and the silicon tubes filled without muscle were sutured in place. In group IV, 2-cm segments of the nerve were removed and the silicon tubes filled with autogenous skeletal muscle were sutured in place. In group V, 2-cm segments of the nerve were removed and the silicon tubes filled without muscle were sutured in place. At 16th week, the eletromyography, the light and transmission electron microscopy were performed. Nerve conduction study stimulating sciatic nerve proximal to the lesion and recording at gastrocnemius muscle showed that the compound muscle action potentials of the group II with 1 cm nerve defect filled with muscle were higher amplitudes than the group III without muscle. Compound muscle action potentials of the group IV with 2 cm defect filled with muscle showed similar results in comparison with the group V. The light and transmission electron microscpy showed that a good morphological pattern of nerve regeneration in 1 cm gap than 2 cm and in gap with muscle than gap without muscle.