• 한국응용약물학회:학술대회논문집
• /
• 한국응용약물학회 1995년도 제3회 추계심포지움
• /
• pp.9-15
• /
• 1995

When quiescent cells ate stimulated to enter the cell cycle, the thymidine kinase(TK) gene is transcriptionally activated at the border of Gl and 5. In this report we show that the human TK promoter contains multiple protein-binding sites. By site-directed mutagenesis, we identified a protein-binding site on the human TK promoter requited for conferring Gl-S-regulated transcription to a heterologous promoter and dissociated it functionally from an adjacent protein-binding domain containing an inverted CCAAT motif requited for high basal level expression. Substitution-mutation of this site results in constitutive expression of the neo reporter gene in serum-stimulated fibroblasts, as well as in cells arrested in mid-Gl by a temperature-sensitive mutation. The regulatory domains for the human TK promoter exhibit interesting symmetrical features, including a set of CCAAT motifs and sites similar to the novel Yi protein-binding site recently discovered in the mouse TK promoter. Thus, components of the hTK complex is important for hTK gene regulation.

• Journal of Microbiology
• /
• 제43권2호
• /
• pp.196-203
• /
• 2005

Here, we attempted to evaluate the activity of artificially transconjugated multiple plasmids in 'designer biocatalysts' for the bioremediation of cocontaminated sites under nonselective conditions. We observed profound losses in the percent survivals of artificially transconjugated plasmid activity ($66 - 78\%$ loss immediately after freeze-drying, $99.45 - 99.88\%$ loss by the end of 6 months storage) in reconstituted Pseudomonas sp. KM12TC. Such unpredictable high losses of this particular plasmid appeared to clearly be a deleterious effect. However, even after 6 months of storage, the cells remained able to degrade $95\%$ of phenol within 9 days, and the full effiux of$^%${73} As, as compared to that of the non-freeze-dried cells, was successfully achieved 4 to 9 days later. These results indicate that 'stable designer biocatalysts' can remain viable, even after freeze-drying and 6 months of storage.

• Bulletin of the Korean Chemical Society
• /
• 제30권6호
• /
• pp.1262-1266
• /
• 2009

Thermodynamics of the interaction between Cerium (III) chloride, $Ce^{3+}$, with Human Serum Albumin, HSA, was investigated at pH 7.0 and $27\;{^{\circ}C}$ in phosphate buffer by isothermal titration calorimetry. Our recently solvation model was used to reproduce the enthalpies of HSA interaction by $Ce^{3+}$. The solvation parameters recovered from our new model, attributed to the structural change of HSA and its biological activity. The interaction of HSA with $Ce^{3+}$ showed a set of two binding sites with negative cooperativity. $Ce^{3+}$ interacts with multiple sites on HSA affecting its biochemical and biophysical properties.

• 방사성폐기물학회지
• /
• 제19권4호
• /
• pp.533-546
• /
• 2021

Large earthquakes with (M_W > ~ 6) result in ground shaking, surface ruptures, and permanent deformation with displacement. The earthquakes would damage important facilities and infrastructure such as large industrial establishments, nuclear power plants, and waste disposal sites. In particular, earthquake ruptures associated with large earthquakes can affect geological and engineered barriers such as deep geological repositories that are used for storing hazardous radioactive wastes. Earthquake-driven faults and surface ruptures exhibit various fault zone structural characteristics such as direction of earthquake propagation and rupture and asymmetric displacement patterns. Therefore, estimating the respect distances and hazardous areas has been challenging. We propose that considering multiple parameters, such as fault types, distribution, scale, activity, linkage patterns, damage zones, and respect distances, enable accurate identification of the sites for deep geological repositories and important facilities. This information would enable earthquake hazard assessment and lower earthquake-resulted hazards in potential earthquake-prone areas.

• Asia pacific journal of information systems
• /
• 제30권1호
• /
• pp.53-71
• /
• 2020

The Internet and all of its possibilities and applications have changed individuals' lifestyles in relation to socializing, working, and how they spend their leisure time. Social networking sites (SNSs), such as Facebook or Instagram, are ideal settings for interacting with others but, unfortunately, they are also ideal settings for motivated offenders to commit cybercrimes. Thus SNS users may be more vulnerable to cybercrime. The purpose of this study is to investigate the occurrence of cybercrime victimization, specifically cyber-harassment, cyber-impersonation, and hacking. Self-report surveys collected from a sample of 147 respondents were examined using the moderated multiple regression analysis and a logistic regression analysis to determine possible relationships between SNS lifestyle exposure activities and cybercrime victimization. The results indicate moderate support for the application of lifestyle-routine activity theory (LRAT) to cybercrime victimization. Possible educational and managerial implications, as well as suggestions for future research, are discussed.

• 한국생물물리학회:학술대회논문집
• /
• 한국생물물리학회 1996년도 정기총회 및 학술발표회
• /
• pp.24-24
• /
• 1996

SecA protein of E. coli is essential for the translocation of various precursor proteins across the plasma membrane. Along with it, SecA protein interacts with precursor proteins, SecY/E, SecB and is an ATPase which has multiple ATP binding sites. There is little known about the regulation mechanism of the protein translocation machinery. (omitted)

• 한국수자원학회:학술대회논문집
• /
• 한국수자원학회 2012년도 학술발표회
• /
• pp.961-964
• /
• 2012

본 연구에서는 1혈장 2음택터의 조성이 발복특성에 미치는 공학적인 접근을 나타내고 있다. 일반적으로 우리나라에서는 하나의 혈장에서는 하나의 음택터를 조성하여 왔다. 그러나 특반급에 해당하는 몇몇의 명당들은 2개의 음택터를 조성한 경우를 알 수 있다. 조선시대 광산김씨 허주 김반대감의 음택터의 경우 특반급에 해당하는 명당으로 1혈장에 2음택터가 조성되어 있다. 본 허주 김반대감 음택터의 공학적인 접근을 통하여 다중음택터의 발복특성을 분석하고자 한다.

• 대한약학회:학술대회논문집
• /
• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
• /
• pp.275.1-275.1
• /
• 2002

It has been proposed that G protein interacts with receptor via multiple interaction sites. With regard to this, C-terminus of the G${\alpha}$ subunit is clearly not the only structural determinant on the G proteins that is critical for receptor coupling selectivity, but the extreme N-terminus of Ga subunit and other structural elements were proposed to be responsible for dictating the interaction with receptors. (omitted)

• Journal of Applied Biological Chemistry
• /
• 제44권4호
• /
• pp.194-198
• /
• 2001

Contaminated sites are often abandoned for an extended period of time, thus organopollutants becoming sequestered. The information on the initial concentrations of the contaminants would be helpful for the economic bioremediation of the chemicals. The present study estimated the initial concentrations of atrazine and phenanthrene through multiple regression analyses using soil properties and the amount of chemicals available in situ. Percentage mineralized or extracted was best correlated with organic C or logarithm of organic C, and the $R^2$ values were 0.548 and 0.894 for atrazine and phenanthrene, respectively. Estimation of the initial concentration of the chemicals was then calculated from both the percentage mineralized or extracted and the amount of chemicals extracted or mineralized. Results showed that the estimation of the initial concentration of the chemical at the time of contamination is feasible.

• Nuclear Medicine and Molecular Imaging
• /
• 제41권3호
• /
• pp.258-259
• /
• 2007

Benign metastasizing leiomyoma(BML) is a rare condition affecting women with a history of having undergone hysterectomy or myomectomy for a benign uterine fibroid, that is found to have metastasized to extrauterine sites, usually many years after hysterectomy. 1,2) Patient with BML almost always asymptomatic, although if the lesions are large enough, they can cause compressive symptoms. Among several hypothesis of pathogenesis, most plausible theory is that these tumors represent a true metastatic lesion but are very low-grade sarcoma. 3) Because the tumor is responsive to estrogen, menopause and pregnancy have slowed the growth of these lesion 4) and it seems reasonable to perform hysterectomy in patients with a uterine mass and, at the same time, perform oophorectomy for hormonal control. BML is an unusual cause of diffuse pulmonary nodules which should be considered in females with unexplained nodules and a history of surgery for uterine leiomyoma.