• Reproductive and Developmental Biology
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• v.37 no.4
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• pp.219-224
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• 2013

Muscular satellite cell (SC), which is stem cell of postnatal pig, is an important for study of differentiation into adipogenesis, myogenesis, and osteoblastogenesis. In this study, we isolated and examined from pig muscle tissue to determine capacity in proliferate, differentiate, and expression of various genes. Porcine satellite cells (PSC) were isolated from semimembranosus (SM) muscles of 90~100 days old pigs according to standard conditions. The cell proliferation increased in multi-potent cell by Masson's, oil red O, and Alizarin red staining respectively. We performed the expression levels of differentiation related genes using real-time PCR. We found that the differentiation into adipocyte increased expression levels of both fatty acid binding protein 4 (FABP4) and peroxisome proliferator-activated receptor gamma ($PPAR{\gamma}$) genes (p<0.01). Myocyte increased the expression levels of the myosin heavy chain (MHC), myogenic factor 5 (Myf5), myogenic regulatory factor (MyoD), and Myogenic factor 4 (myogenin) (p<0.01). Osteoblast increased the expression levels of alkaline phosphatase (ALP) (p<0.01). Finally, porcine satellite cells were induced to differentiate towards adipogenic, myogenic, and osteoblastogenic lineages. Our results suggest that muscle satellite cell in porcine may influence cell fate. Understanding the progression of PSC may lead to improved strategies for augmenting meat quality.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.17 no.12
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• pp.2806-2811
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• 2013

New technologies such as multi-antenna and small cell were proposed as key technology for the next generation mobile system to cope with the explosively increasing mobile data traffic. In particular, heterogeneous mobile communication network which can improve spatial reuse factor by exploiting macro and small cells simultaneously is attracting attention. However, the heterogeneous network has a problem that the utilization of small cells becomes low because the transmit power of macro base stations is much higher than that of small base stations and then the probability that mobile stations are attached to the macro base stations becomes high. This problem is dominant in uplink. The concept of cell range expansion bias to mitigate the problem was proposed by 3GPP and the corresponding standardization is in progress. In this paper, we analyze the downlink performance of the heterogeneous mobile communication network based on a system level simulator with the cell range expansion bias in terms of average cell spectral efficiency.

• Laboraroty Animal Research
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• v.34 no.4
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• pp.302-310
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• 2018

CD47 (integrin-associated protein), a multi-spanning transmembrane protein expressed in all cells including red blood cells (RBCs) and leukocytes, interacts with signal regulatory protein ${\alpha}$ ($SIRP{\alpha}$) on macrophages and thereby inhibits phagocytosis of RBCs. Recently, we generated a novel C57BL/6J CD47 knockout ($CD47^{-/-}$ hereafter) mouse line by employing a CRISPR/Cas9 system at Center for Mouse Models of Human Disease, and here report their hematological phenotypes. On monitoring their birth and development, $CD47^{-/-}$ mice were born viable with a natural male-to-female sex ratio and normally developed from birth through puberty to adulthood without noticeable changes in growth, food/water intake compared to their age and sex-matched wild-type littermates up to 26 weeks. Hematological analysis revealed a mild but significant reduction of RBC counts and hemoglobin in 16 week-old male $CD47^{-/-}$ mice which were aggravated at the age of 26 weeks with increased reticulocyte counts and mean corpuscular volume (MCV), suggesting hemolytic anemia. Interestingly, anemia in female $CD47^{-/-}$ mice became evident at 26 weeks, but splenomegaly was identified in both genders of $CD47^{-/-}$ mice from the age of 16 weeks, consistent with development of hemolytic anemia. Additionally, helper and cytotoxic T cell populations were considerably reduced in the spleen, but not in thymus, of $CD47^{-/-}$ mice, suggesting a crucial role of CD47 in proliferation of T cells. Collectively, these findings indicate that our $CD47^{-/-}$ mice have progressive hemolytic anemia and splenic depletion of mature T cell populations and therefore may be useful as an in vivo model to study the function of CD47.

• Animal Bioscience
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• v.36 no.1
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• pp.29-42
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• 2023

Objective: Pigs, an ideal biomedical model for human diseases, suffer from about 50% early embryonic and fetal death, a major cause of fertility loss worldwide. However, identifying the causal variant remains a huge challenge. This study aimed to detect single nucleotide polymorphisms (SNPs) and candidate genes for the number of mummified (NM) piglets using the imputed whole-genome sequence (WGS) and validate the potential candidate genes. Methods: The imputed WGS was introduced from genotyping-by-sequencing (GBS) using a multi-breed reference population. We performed genome-wide association studies (GWAS) for NM piglets at birth from a Landrace pig populatiGWAS peak located on SSC11: 0.10 to 7.11 Mbp (Top SNP, SSC11:1,889,658 bp; p = 9.98E-13) was identified in cyclin dependent kinase on. A total of 300 Landrace pigs were genotyped by GBS. The whole-genome variants were imputed, and 4,252,858 SNPs were obtained. Various molecular experiments were conducted to determine how the genes affected NM in pigs. Results: A strong GWAS peak located on SSC11: 0.10 to 7.11 Mbp (Top SNP, SSC11:1,889,658 bp; p = 9.98E-13) was identified in cyclin dependent kinase 8 (CDK8) gene, which plays a crucial role in embryonic retardation and lethality. Based on the molecular experiments, we found that Y-box binding protein 1 (YBX1) was a crucial transcription factor for CDK8, which mediated the effect of CDK8 in the proliferation of porcine ovarian granulosa cells via transforming growth factor beta/small mother against decapentaplegic signaling pathway, and, as a consequence, affected embryo quality, indicating that this pathway may be contributing to mummified fetal in pigs. Conclusion: A powerful imputation-based association study was performed to identify genes associated with NM in pigs. CDK8 was suggested as a functional gene for the proliferation of porcine ovarian granulosa cells, but further studies are required to determine causative mutations and the effect of loci on NM in pigs.

• Proceedings of the KIEE Conference
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• 1999.07d
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• pp.1870-1872
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• 1999

Performance of 20-cell stack for direct methanol fuel cell (DMFC) was tested at constant temperature. Electrode evaluation used to the stack was tested by the performance of a single cell. A new composite electrode prepared from active carbon cloth and high porous active carbon was developed for hydrophilic layer of the cell. Characteristics of a single cell using the composite electrode showed the current density of $500mA/cm^2$ at the cell voltage of 0.4V at $120^{\circ}C$. For the operating of 20 days. the cell voltage at constant cell current densty of $100mA/cm^2$ was slightly reduced from 0.62V to 0.53V with the cell voltage decay rate of 14.5%. Power of 20-cell stack at 5.3V, $100^{\circ}C$ was about 180W.

• Solar Energy
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• v.10 no.3
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• pp.46-52
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• 1990

Novel a-Si solar cells with delta-doped(${\delta}x$-doped) P-layer have been fabricated to enhance the hole concentration of the P-layers. The ${\delta}-$doped P-layer consists of very thin B sheets of 0.1-0.5 atomic layers and undoped a-SiC multi-layers. B-layers were prepared by photo-CVD and pyrolysis technique. The structural, optical and electrical characteristics of the delta-doped P-layer films were evaluated by means of FTIR, AES and SIMS. As the results of this study, it was found that the ${\delta}$-doped P-layer showed much superior optical and electrical characteristics than those of conventional uniformly B-doped a-Si layers. 12.5% energy conversion efficiency was achieved for the Cell with ${\delta}$-doped P-layer.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.32 no.2A
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• pp.179-187
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• 2007

As wireless networks evolve to orthogonal frequency division multiple access(OFDMA) systems, inter-cell interference control becomes a critical issue in radio resource management. The allocation of the same channels in neighbor cells cause inter-cell interference, so the channel allocation needs to be taken carefully to lower the inter-cell interference. In distributed channel allocation, each cell independently tries to allocate channels that suffer low interference level. In this paper, under the assumption of static users, we introduce the concept of interference range and use it in designing our two algorithms; basic and combined. The basic algorithm performs interference range detection and determines whether to use the considered channel, while the combined algorithm checks the channel quality in addition to detecting the interference range. The two algorithms dynamically perform channel allocation with low complexity and show good throughput and fairness performance.

• Molecules and Cells
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• v.39 no.5
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• pp.389-394
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• 2016

Dovitinib (TKI258) is a small molecule multi-kinase inhibitor currently in clinical phase I/II/III development for the treatment of various types of cancers. This drug has a safe and effective pharmacokinetic/pharmacodynamic profile. Although dovitinib can bind several kinases at nanomolar concentrations, there are no reports relating to osteoporosis or osteoblast differentiation. Herein, we investigated the effect of dovitinib on human recombinant bone morphogenetic protein (BMP)-2-induced osteoblast differentiation in a cell culture model. Dovitinib enhanced the BMP-2-induced alkaline phosphatase (ALP) induction, which is a representative marker of osteoblast differentiation. Dovitinib also stimulated the translocation of phosphorylated Smad1/5/8 into the nucleus and phosphorylation of mitogen-activated protein kinases, including ERK1/2 and p38. In addition, the mRNA expression of BMP-4, BMP-7, ALP, and OCN increased with dovitinib treatment. Our results suggest that dovitinib has a potent stimulating effect on BMP-2-induced osteoblast differentiation and this existing drug has potential for repositioning in the treatment of bone-related disorders.

• Bulletin of the Korean Chemical Society
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• v.31 no.2
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• pp.331-334
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• 2010

In the present study, carbon supports mixed with purified multi-walled carbon nanotubes (MWNTs) and carbon blacks (CBs) were used to improve the cell performance of direct methanol fuel cells (DMFCs). Additionally, the effect of $O_2$ plasma treatment on CBs/MWNTs supports was investigated for different plasma RF powers of 100, 200, and 300 W. The surface and structural properties of the CBs/MWNTs supports were characterized by FT-IR, X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and inductive coupled plasma-mass spectrometer (ICP-MS). The electrocatalytic activity of PtRu/CBs/MWNTs catalysts was investigated by cyclic voltammetry measurement. In the experimental results, the oxygen functional groups of the supports were increased with increasing plasma RF power, while the average Pt particle size was decreased owing to the improvement of dispersibility of the catalysts. The electrochemical activity of the catalysts for methanol oxidation was gradually improved by the larger available active surface area, itself due to the introduction of oxygen functional groups. Consequently, it was found that $O_2$ plasma treatments could influence the surface properties of the carbon supports, resulting in enhanced electrocatalytic activity of the catalysts for DMFCs.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.11 no.10
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• pp.4759-4780
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• 2017

Full Dimension multiple input multiple output (FD-MIMO) architecture employs a planar array design at the Base Station (BS) to provide high order multi-user MIMO (MU-MIMO) via simultaneous data transmission to large number of users. With FD-MIMO, the BS can also adjust the beam direction in both elevation and azimuth direction to concentrate the energy on the user of interests while minimizing the interference leakage to co-scheduled users in the same cell or users in the neighboring cells. In a typical highly populated macrocell environment, modelling the elevation angular characteristics of three-dimensional (3D) channel is critical to understanding the performance limits of the FD-MIMO system. In this paper, we study the throughput performance of FD-MIMO system with varying elevation angular spread and inter-element spacing using a 3D spatial channel model. Our results show that for a typical urban scenario, horizontal beamforming with correlated antenna spacing achieves optimal performance but by restricting the spread of elevation angles of departure, elevation beamforming achieves high array gain with wide inter-element spacing. We also realize significant gains due to spatial array processing via modelling the elevation domain and varying the inter-element spacing for both the transmitter and receiver.