• The Transactions of the Korean Institute of Power Electronics
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• v.23 no.4
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• pp.247-255
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• 2018

This paper proposes a transformer design of a direct cell-to-cell active cell balancing circuit with a multi-winding transformer for battery management system (BMS) applications. The coupling coefficient of the multi-winding transformer and the output capacitance of MOSFETs significantly affect the balancing current transfer efficiency of the cell balancing operation. During the operation, the multi-winding transformer stores the energy charged in a specific source cell and subsequently transfers this energy to the target cell. However, the leakage inductance of the multi-winding transformer and the output capacitance of the MOSFET induce an abnormal energy transfer to the non-target cells, thereby degrading the transfer efficiency of the balancing current in each cell balancing operation. The impacts of the balancing current transfer efficiency deterioration are analyzed and a transformer design methodology that considers the coupling coefficient is proposed to enhance the transfer efficiency of the balancing current. The efficiency improvements resulting from the selection of an appropriate coupling coefficient are verified by conducting a simulation and experiment with a 1 W prototype cell balancing circuit.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.34 no.6
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• pp.588-593
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• 2008

There are increasing reports regarding regeneration of the defected tissues using tissue engineering technique. In this technique, multipotential stem cells are essential. There are many potential sources of adult stem cells, such as bone marrow, umbilical cord blood, fat, muscle, dental tissues and skin. Among them, skin is highly accessible and easily obtained with a minimum of donor site complications. Moreover, skin is an abundant adult stem cell sources and has the potential for self-replication and immune privilege. In this study, we isolated skin-derived precursor cells (SKPs) from the ear of adult miniature pigs. In these SKPs, the expression of transcriptional factors, Oct-4, Sox-2, and Nanog were detected by RT-PCR. In vitro osteogenesis and adipogenesis were observed at 3 weeks after transdifferentiations as assayed by positive von Kossa and Oil-red O staining, respectively. In addition, expression of osteocalcin and osteonectin in the osteogenic differentiation medium and $PPAR{\gamma}2$ and aP2 in the adipogenic differentiation medium were detected by RT-PCR. In vitro neurogenesis of porcine SKPs was observed during 24 and 72 hours after treatment of neurogenic differentiation medium. The results of this study suggest that SKPs demonstrate the properties of pluripotence or multipotence and multi-lineage differentiation. This indicates that autogenous SKPs are a reliable and useful source of adult stem cells for regenerative medicine.

• Molecules and Cells
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• v.41 no.11
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• pp.971-978
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• 2018

The stem cell factor (SCF)/c-KIT axis plays an important role in the hematopoietic differentiation of human pluripotent stem cells (hPSCs), but its regulatory mechanisms involving microRNAs (miRs) are not fully elucidated. Here, we demonstrated that supplementation with SCF increases the hematopoietic differentiation of hPSCs via the interaction with its receptor tyrosine kinase c-KIT, which is modulated by miR-221 and miR-222. c-KIT is comparably expressed in undifferentiated human embryonic and induced pluripotent stem cells. The inhibition of SCF signaling via treatment with a c-KIT antagonist (imatinib) during hPSC-derived hematopoiesis resulted in reductions in the yield and multi-lineage potential of hematopoietic progenitors. We found that the transcript levels of miR-221 and miR-222 targeting c-KIT were significantly lower in the pluripotent state than they were in terminally differentiated somatic cells. Furthermore, suppression of miR-221 and miR-222 in undifferentiated hPSC cultures induced more hematopoiesis by increasing c-KIT expression. Collectively, our data implied that the modulation of c-KIT by miRs may provide further potential strategies to expedite the generation of functional blood cells for therapeutic approaches and the study of the cellular machinery related to hematologic malignant diseases such as leukemia.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.32 no.6
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• pp.495-505
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• 2006

In this study we evaluate the effects of bFGF-BB and PDGF on in vitro proliferation, differentiation and mineralization of mesenchymal stem cells (MSCs) from rat. MSCs were prepared from the bone marrow of 6 or 7-week-old male rats with a technique previously described by Maniatopoulos et al. in 1988. Lineage differentiation to osteogenesis, chondrogenesis and adipogenesis were performed. At first, we characterized the cultured cell on passage 1, 3, 5, 7 with immunocytochemical staining using CD29, 44, 34, 45, ${\alpha}$-SMA and type I collagen. And to study the effects of bFGF and PDGF-BB on proliferation, differentiation and mineralization, we seeded the expanded cell at a density of 6 $6{\times}10^3\;cells/cm^2$ to 100-mm dish for evaluation of cell proliferation and MTT assay was carried out on day 2, 4, 7, 9. We also resuspended the cells with same density $(6{\times}10^3\;cells/cm^2)$ to 24 well plates for subculture. On the following day, the attached cells were exposed to 2.5ng/ml bFGF and/or 25ng/ml PDGF-BB daily during 5 days. The osteocalcin (OC) level was assessed and mineral contents were evaluated with alizarin red S staining on subculture day 2, 7, 14, 21. We identified the mesenchymal stem cell from the bone marrow derived cells of rat through their successful multi-differentiation and stable display of its phenotype. And bFGF and PDGF-BB showed the effect that inhibited osteoblastic differentiation and mineralization mildly in above concentration at in vitro culture. This study was supported by grant 04-2004-0120 from the Seoul National University Hospital Research Fund.

• Biomolecules & Therapeutics
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• v.29 no.6
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• pp.658-666
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• 2021

Podophyllotoxin (PT), a lignan compound from the roots and rhizomes of Podophyllum peltatum, has diverse pharmacological activities including anticancer effect in several types of cancer. The molecular mechanism of the anticancer effects of PT on colorectal cancer cells has not been reported yet. In this study, we sought to evaluate the anticancer effect of PT on human colorectal cancer HCT116 cells and identify the detailed molecular mechanism. PT inhibited the growth of cells and colony formation in a concentration-dependent manner and induced apoptosis as determined by the annexin V/7-aminoactinomycin D double staining assay. PT-induced apoptosis was accompanied by cell cycle arrest in the G2/M phase and an increase in the generation of reactive oxygen species (ROS). The effects of PT on the induction of ROS and apoptosis were prevented by pretreatment with N-acetyl-L-cysteine (NAC), indicating that an increase in ROS generation mediates the apoptosis of HCT116 cells induced by PT. Furthermore, Western blot analysis showed that PT upregulated the level of phospho (p)-p38 mitogen-activated protein kinase (MAPK). The treatment of SB203580, a p38 inhibitor, strongly prevented the apoptosis induced by PT, suggesting that PT-induced apoptosis involved the p38 MAPK signaling pathway. In addition, PT induced the loss of mitochondrial membrane potential and multi-caspase activation. The results suggested that PT induced cell cycle arrest in the G2/M phase and apoptosis through the p38 MAPK signaling pathway by upregulating ROS in HCT116 cells.

• Biomolecules & Therapeutics
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• v.31 no.6
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• pp.661-673
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• 2023

Treatment of colorectal cancer (CRC) has always been challenged by the development of resistance. We investigated the antiproliferative activity of licochalcone H (LCH), a regioisomer of licochalcone C derived from the root of Glycyrrhiza inflata, in oxaliplatin (Ox)-sensitive and -resistant CRC cells. LCH significantly inhibited cell viability and colony growth in both Ox-sensitive and Ox-resistant CRC cells. We found that LCH decreased epidermal growth factor receptor (EGFR) and AKT kinase activities and related activating signaling proteins including pEGFR and pAKT. A computational docking model indicated that LCH may interact with EGFR, AKT1, and AKT2 at the ATP-binding sites. LCH induced ROS generation and increased the expression of the ER stress markers. LCH treatment of CRC cells induced depolarization of MMP. Multi-caspase activity was induced by LCH treatment and confirmed by Z-VAD-FMK treatment. LCH increased the number of sub-G1 cells and arrested the cell cycle at the G1 phase. Taken together LCH inhibits the growth of Ox-sensitive and Ox-resistant CRC cells by targeting EGFR and AKT, and inducing ROS generation and ER stress-mediated apoptosis. Therefore, LCH could be a potential therapeutic agent for improving not only Ox-sensitive but also Ox-resistant CRC treatment.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.7 no.1
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• pp.95-103
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• 1998

This paper is concentraed on a study of operation for multiple objectives in a automated manufacturing system with multiple machining cells . Largest Sum Processing-time First(LSPF) was developed in order to minimize makespan, mean flowtime and maximize mean utilization and compare with Ho and Chang's algorithm(HC) and Hunsucker and Shah's algorithm(HS). The results show that LSPF provides better soutions than HC at 78.2% and than HS at 67.8% of total problems to frequency . LSPF reduces 5.8% of makespan by HC and 22.1% of the value by HS and curtails 15.8% , 7.5% of mean flowtime by receptive algorithms(HC, HS). And mean utilization is also higher about 5.5% than HC and HS.

• Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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• 2007.11a
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• pp.319-322
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• 2007

In the development of paralleling techniques, Multi-Phase Interleaved(MPI) converter constitutes one of the most promising alternatives reported in the last years. This technique consists of a phase shifting of the control signals of several cells in parallel operating at the same switching frequency. As a result, the aggregated input and output current waveform exhibit lower ripple amplitude and smaller harmonics content than in synchronous or stochastic operation modes. Based on the inherent advantages of the MPI converter, in this paper, a control scheme, which can reduce current and voltage rifle, is proposed for PEMFC generation systems. The MPI boost converter is composed of several identical boost converters connected in parallel.

• The Transactions of the Korean Institute of Electrical Engineers D
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• v.49 no.12
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• pp.675-682
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• 2000

When a system is composed of multi-boards, an efficient bus arbitration method for the data transfer bus must be provided for guaranteeing proper operations. In this paper, a new test methodology is developed which is used for testing on-line bus arbitration. In the new test methodology, events that are occurred during bus arbitration are defined, and expected signals during fault-free bus arbitration are compared with the signals captured during on-line bus arbitration using boundary-scan cells. For this, a new test architecture is proposed which is efficient for the maintenance and the repair of multi-board systems. In addition, the new methodology can be used with off-line interconnect test using boundary-scan.

• 한국HCI학회:학술대회논문집
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• 2008.02a
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• pp.623-628
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• 2008

We present a new method of extracting foreground object from background image for vision-based game interface. Background Subtraction is an important preprocessing step for extracting the features of tracking objects. The image is divided into the cells where the Local Histogram with Gaussian kernel is computed and compared with the corresponding one using Bhattacharyya distance measure. The histogram-based method is partially robust against illumination change, noise and small moving objects in background. We propose a Multi-Scaled Integral Histogram approach for noise suppression and fast computation.