• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.29 no.1
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• pp.47-64
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• 2016

Objective : This study was carried out to investigate the effect of Hwangryeonhaedoktang pharmacopuncture solution (HRHDT) and microneedle therapy system (MTS) on hair growth in an alopecia model of C57BL/6N mice.Methods : Six-week old mice were depilated and separated in 4 groups ; CON (saline), MXD (3% Minoxidil), MTS and HRHDT+MTS. The treatments were applied twice a week for 17 days. The hair growth was determined photographically. The hair density, thickness and length were identified by Folliscope and the weights of body and organs were measured. In dorsal skin tissue, the expression of hair growth-related gene and protein was analyzed by RT-PCR. In addition, the hair follicles in the dermis were observed by H&E staining.Results : The promotion of hair growth was observed in HRHDT+MTS and MTS compared to CON. The hair density, thickness and length were also improved in HRHDT+MTS and MTS compared to CON. The mRNA expression of IGF-1, PRL and PL and the protein expression of VEGF and IGF-1 were increased in HRHDT+MTS and MTS compared to CON. The hair follicles and hair root growth were improved in HRHDT+MTS and MTS compared to CON. In the above results, HRHDT+MTS were more effective than MTS.Conclusions : These results suggest that HRHDT and MTS have a hair growth activity and can be useful for the treatment of alopecia.

• Journal of Korean Society for Quality Management
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• v.33 no.2
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• pp.64-73
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• 2005

In this paper, we introduce MTS(Mahalanobis-Taguchi System) Method which is suggested Dr. Taguchi in later 1990. We apply MTS Method for Quality Competitive Appraisal System(QCAS) Model which is executived from 1997 by Agency for Technology and Standards, Ministry of Commerce, Industry and Energy(MOCIE). We can measure company Quality competitive by using MTS. MTS Measure can not be compared statistical sum by calculated QCAS. MTS can be possible distinct subtle which can not distinct using statistical sum. Also MTS Method can seek more strong effect factor among of many factor. If A Company use MTS Method, can find vital factor and level of destination.

• The Journal of Pediatrics of Korean Medicine
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• v.28 no.2
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• pp.72-87
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• 2014

Objectives This study was carried out to investigate the effect of Ganoderma lucidum extract (GLE) and microneedle therapy system (MTS) on hair growth in an alopecia model of C57BL/6N mice. Methods Five-week old mice were depilated and separated in 4 groups ; CON (50% EtOH), MXD (5% Minoxidil), MTS and GLE + MTS. The treatments were applied twice a week for 3 weeks. The hair growth was determined photographically, the hair density, thickness and length were identified by Folliscope and the weights of body and organs were measured. In dorsal skin tissue, the expression of hair growth-related gene and protein was analyzed by RT - PCR or Western blot. In addition, the hair follicles in the dermis were observed by H&E staining. Results The promotion of hair growth was observed in GLE + MTS and MTS compared to CON. The hair density, thickness and length were also improved in GLE + MTS and MTS compared to CON. The mRNA expression of TGF-${\alpha}$, TGF-${\beta}1$, IGF-1, PRL and PL and the protein expression of VEGF and IGF-1 were increased in GLE + MTS and MTS compared to CON. The hair follicles and hair root growth were improved in GLE + MTS and MTS compared to CON. In the above results, GLE + MTS were more effective than MTS. Conclusions These results suggest that GLE and MTS has a hair growth activity and can be useful for the treatment of alopecia.

• Korean journal of food and cookery science
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• v.26 no.6
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• pp.866-871
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• 2010

The antioxidant activities and quality characteristics of jams containing maltitol substituted for sucrose(sucrose, maltitol syrup(MT), and MTS(sucrose 50%+MT 50%)) were evaluated. The endpoint of jam preparation was determined to be 64 $^{\circ}Brix$. The pH was the highest in MTS among all treatments. The reducing sugar content and viscosity were the highest in MT, and second highest in MTS. Lightness(L value) of Hunter color system was the highest in MT, whereas yellowness(b value) was the highest in MTS. Hardness and cohesiveness of textural properties were the highest in MT. Antioxidant activities were the highest in MT, which also had the lowest $IC_{50}$ values(49.63 mg/g for DPPH and 27.24 mg/g for hydroxyl radical scavenging activity). Total phenol content was highest in MT among all treatments. Flavor and overall acceptance scores of MTS were the highest among all treatments. Based on these results, it was suggested that half-substitution of sucrose with maltitol is the most appropriate for attaining favorable physicochemical and antioxidative properties during black garlic jam preparation.

• The Korea Journal of Herbology
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• v.36 no.4
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• pp.31-39
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• 2021

Objectives : The purpose of this study was to find out the efficacy of ginseng on the skin by comparing the difference effect between only MTS (microneedle therapy system) and the ginseng solution application after MTS treatment. Methods : The present author performed once a week and total 3 times of MTS treatment. The treatment time took about 5~10 minutes and the depth of the needle was adjusted according to the participant's skin condition. In the control group, skin toner was applied after MTS and in the experimental group, ginseng extract dissolved toner was applied. One week after each treatment, the change of skin condition was measured. Two weeks after the end of the clinical trial, the skin condition was checked again. Results : In the case of t-zone oil, there was a significant decrease in the test group and in the case of pores and pigmentation, there was a significant decrease in both the test group and the control group, but the degree of significance was greater in the test group. In the case of skin tone, there was an increase, but no significance. Conclusion : The MTS treatment itself showed good effects on t-zone oil, pores, pigmentation, and skin tone. But the effect was much better when the ginseng extract solution was applied to the skin after MTS treatment. This study suggests that ginseng solution increases the efficacy of MTS.

• The Korea Journal of Herbology
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• v.37 no.2
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• pp.23-31
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• 2022

Objectives : The purpose of this research was to find out the efficacy of Castanea crenata Inner Shell (CCIS) on the skin by comparing the difference effect between only MTS (microneedle therapy system) and the CCIS essence application after MTS treatment. Methods : The present author performed once a week and total 3 times of MTS treatment. The treatment time took about 5 ~ 10 minutes and the depth of the needle was adjusted according to the participant's skin condition. In the control group, essence base was applied after MTS and in the experimental group, CCIS powder dissolved essence was applied. One week after each treatment, the change of skin condition was measured. Two weeks after the end of the clinical trial, the skin condition was checked again. Results : In the case of the test group and the control group, there was a significant decrease in thermography temperature and there was a significant increase in skin tone. In the case of pores and pigmentation, there was no significant difference on both group. In the case of moisture, there was a significant increase only in the test group. Conclusion : The MTS treatment itself showed good effects on thermography temperature and skin tone. But in the case of the moisture effect, it was much better when the CCIS essence was applied to the skin after MTS treatment. This study suggests that CCIS essence increases the efficacy of MTS on the point of moisture.

• The Korean Journal of Physiology and Pharmacology
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• v.26 no.1
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• pp.15-24
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• 2022

The development of selective targeting of drug molecules towards the mitochondria is an important issue related to therapy efficacy. In this study, we report that gallic acid (GA)-mitochondria targeting sequence (MTS)-H₃R₉ exhibits a dual role as a mitochondria-targeting vehicle with antioxidant activity for disease therapy. In viability assays, GA-MTS-H₃R₉ showed a better rescue action compared to that of MTS-H₃R₉. GA-MTS-H₃R₉ dramatically exhibited cell penetration and intercellular uptake compared to MTS and fit escape from lysosome release to the cytosol. We demonstrated the useful targeting of GA-MTS-H₃R₉ towards mitochondria in AC16 cells. Also, we observed that the antioxidant properties of mitochondrial-accrued GA-MTS-H₃R₉ alleviated cell damage by reactive oxygen species production and disrupted mitochondrial membrane potential. GA-MTS-H₃R₉ showed a very increased cytoprotective effect against anticancer activity compared to that of MTS-H₃R₉. We showed that GA-MTS-H₃R₉ can act as a vehicle for mitochondria-targeting and as a reagent for therapeutic applications intended for cardiovascular disease treatment.

• Journal of Life Science
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• v.29 no.1
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• pp.9-17
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• 2019

As tumors develop, they encounter microenvironmental stress, such as hypoxia and glucose depletion, due to poor vascular function, thereby leading to necrosis, which is observed in solid tumors. Necrotic cells are known to release cellular cytoplasmic contents, such as high mobility group box 1 (HMGB1), into the extracellular space. The release of HMGB1, a proinflammatory and tumor-promoting cytokine, plays an important role in promoting inflammation and metabolism during tumor development. Recently, HMGB1 was shown to induce the epithelial-mesenchymal transition (EMT) and metastasis. However, the underlying mechanism of the HMGB1-induced EMT, invasion, and metastasis is unclear. In this study, we showed that noninvasive breast cancer cells MCF-7 formed tightly packed, rounded spheroids and that the cells in the inner regions of a multicellular tumor spheroid (MTS), an in vitro model of a solid tumor, led to necrosis due to an insufficient supply of O2 and glucose. In addition, after 7 d of MTS culture, the EMT was induced via the transcription factor Snail. We also showed that HMGB1 receptors, including RAGE, TLR2, and TLR4, were induced by MTS culture. RAGE, TLR2, and TLR4 shRNA inhibited MTS growth, supporting the idea that RAGE/TLR2/TLR4 play critical roles in MTS growth. They also prevented MTS culture-induced Snail expression, pointing to RAGE/TLR2/TLR4-dependent Snail expression. RAGE, TLR2, and TLR4 shRNA suppressed the MTS-induced EMT. In human cancer tissues, high levels of RAGE, TLR2, and TLR4 were detected. These findings demonstrated that the HMGB-RAGE/TLR2/TLR4-Snail axis played a crucial role in the growth of the MTS and MTS culture-induced EMT.

• Journal of Applied Biological Chemistry
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• v.43 no.4
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• pp.240-245
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• 2000

Two genes encoding maltooligosyltrehalose synthase (SaMTS) and maltooligosyltrehalose trehalohydrolase (SaMTH) were isolated from a hyperthermophilic microorganism, Sulfolobus acidocaldarius (ATCC 49462). ORFs of the SaMTS and SaMTH genes are 2,163 and 1,671 bp long and encode 720 and 556 amino acid residues, respectively. A bifunctional fusion enzyme (SaMTSH) was constructed through the gene fusion of SaMTS and SaMTH. Recombinant SaMTS, SaMTH, and SaMTSH fusion enzyme were overexpressed in E. coli BL21. SaMTS and SaMTH produced trehalose and maltotriose from maltopentaose in a sequential reaction. SaMTSH fusion enzyme catalyzed the sequential reaction in which the formation of maltotriosyltrehalose was followed by hydrolysis leading to the synthesis of trehalose and maltotriose. The SaMTSH fusion enzyme showed the highest activity at pH 5.0-5.5 and $70-75^{\circ}C$. SaMTS, SaMTH, and SaMTSH fusion enzyme were active in soluble starch, which resulted in the production of trehalose.

• Nutritional Sciences
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• v.8 no.3
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• pp.169-174
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• 2005

This study investigated the effect of allium vegetable intake on the storage and utilization of energy substrates before, during, and after exercise in tissues of rats. Ninety mts were fed either a control diet or a diet with added allium sativum (AS), allium cepa (AC), allium fistulosum (AF), or allium tuberosum (AT) for 4 weeks and then subdivided into 3 groups: before-exercise (BE) during-exercise (DE) after-exercise (AE). The DE group exercised on treadmill for 1 hour just before being sacrificed at the end of the 4th week of the dietary treatment Rats in the AE group were allowed to recuperate for 2 horns after being exercised like the DE group. Pyriooxal 5'-phospwe (PLP) levels were compared in plasma, liver and skeletal muscle of rats. There was no difference between AS animals and control animals in plasma PLP levels regardless of exercise. The plasma PLP levels of AC animals were higher than those of control animals before exercise but this PLP was decreased with exercise and lower than that of control animals after exercise. The plasma PLP levels of AF animals were higher than those of control animals during exercise but there was no difference before and after exercise. The plasma PLP levels of AT animals were higher than those of control animals regardless of exercise. Compared to those of control mts, the PLP levels of liver and muscle were significantly lower in AS, AC, AF and AT mts before exercise. The levels of liver PLP were significantly decreased in control mts while not changed in AS, AC, AF and AT mts during exercise. The levels of liver PLP tended to decrease in AS, AC and AF mts after exercise. The levels of muscle PLP were significantly decreased in control rats, while not changed in AS, AC and AF mts during exercise. The levels of muscle PLP were decreased in control mts but not changed in AS, AC and AF mts after exercise. Thus, it is suggested that the changes of PLP concentrations in plasma and tissues induced by exercise are affected by allium vegetable diet and demonstrated that allium vegetable intake induced an alteration in the redistribution of PLP among tissues.