• Title/Summary/Keyword: mtDNA COI

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Identification of Trichiurus (Pisces: Trichiuridae) Eggs and Larvae from Korea, with a Taxonomic Note

  • Lee, Soo Jeong;Kim, Jin-Koo
    • Fisheries and Aquatic Sciences
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    • v.17 no.1
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    • pp.137-143
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    • 2014
  • The hairtail (currently recognized as Trichiurus lepturus in Korea) is one of the most important commercial fish species in Korea, Japan, China, and Taiwan. Because the amount of catches has been steadily declining, we must determine the early life stages of the hairtail from the viewpoint of resource management. Furthermore, the taxonomic status of the hairtail is unclear among ichthyologists, potentially creating management difficulties. Therefore, the purpose of this study was to compare morphological and molecular information on eggs, larvae, and adults of hairtail from Korea with that of T. lepturus from the Atlantic Ocean, and to review the taxonomic status of the hairtail. A total of 510 base pairs of the mitochondrial DNA cytochrome oxidase subunit I sequences of 12 eggs, 2 larvae, and 11 adults of the hairtail from the Korean waters clearly matched those of Trichiurus japonicus adults (d = 0.000-0.014) from the East China Sea rather than those of T. lepturus (d = 0.100-0.110) from the Atlantic Ocean. Our results also showed that larvae of the Korean hairtail are different than those in the Atlantic Ocean in having no melanophores along the ventral edge of the lower jaw. Therefore, our findings suggest that the hairtail in the Korean waters may not be T. lepturus, but T. japonicus.

Vertical Distribution of Icthyoplankton in the Southern Waters of Jeju Island During Spring (봄철 제주 남부해역 난·자치어의 수직 분포)

  • Lee, Bo-Ram;Ji, Hwan-Sung;Yu, Hyo-Jae;Hwang, Kang-Seok;Kim, Doo-Nam
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.55 no.2
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    • pp.146-153
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    • 2022
  • The vertical distribution and abundance of icthyoplankton in the southern waters of Jeju Island during June 2020 were investigated. Fish eggs and larvae were identified using the mitochondrial DNA cytochrome c oxidase subunit I (mtDNA COI) and the 16S rRNA gene. During this period, fish eggs of 23 taxa belonging to 21 families and larvae of 27 taxa belonging to 25 families were collected. Fish eggs were located mostly from the surface to 30 m depth of the water column. Larvae were located from the surface to 80 m depth of the water column. Vertical distributions of fish eggs and larvae were influenced by oceanography conditions such as temperature, salinity, and thermocline depth. No discernible difference in mean thermocline depth was observed between day and night.

Development and validation of ultra-fast quantitative real-time PCR method to differentiate between Oncorhynchus keta and Oncorhynchus mykiss

  • Min-Ji Park;Han-Cheol Lee;Ji-Young Yang;Jung-Beom Kim
    • Food Science and Preservation
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    • v.30 no.3
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    • pp.383-394
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    • 2023
  • The ultra-fast quantitative real-time polymerase chain reaction (qPCR) assay was developed and validated to differentiate the morphologically similar ones, Oncorhynchus keta and Oncorhynchus mykiss. Species-specific primers were designed for the COI genes of mtDNA. The species-specific primers designed for O. keta and O. mykiss were selectively amplified by O. keta and O. mykiss DNA, respectively. The sensitivity of O. keta and O. mykiss primers was 1 ng/μL. Quantitative testing showed that the results met the 'Guidelines on Standard Procedures for Preparing Analysis Method such as Food' proposed by the Ministry of Food and Drug Safety. The qPCR method developed and validated in this study for identifying O. keta and O. mykiss has advantages such as speed and field applicability. Therefore, this method is expected to help control forgery and alteration of raw materials in the seafood industry.

A New Record of the Genus Areotetes (Hymenoptera: Braconidae: Opiinae) from Korea (한국산 미기록속 Areotetes (벌목: 고치벌과: 꽃파리고치벌아과)에 대한 보고)

  • Han, Yunjong;Sohn, JuHyeong;Lim, Jongok;Kim, Hyojoong
    • Korean journal of applied entomology
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    • v.61 no.2
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    • pp.307-311
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    • 2022
  • The genus Areotetes van Achterberg & Li, 2013 (Hymenoptera: Braconidae: Opiinae), which is endoparasitoid of mining or infesting of fruit dipterous larvae, have been reported for the first time in China. Currently, four species of the genus Areotetes have been known from the province Hunan and Fujian, China. In this study the genus Areotetes with Areotetes carinuliferus van Achterberg & Li, 2013 is reported for the first time from Korea. Material studied in the present study were collected by sweeping in Mt Gongchi, Eochungdo, Province Jeonbuk, Korea. Herein, diagnosis of genus, description, distribution, and diagnostic illustration of A. carinuliferus are provided. In addition, DNA barcode data of the partial gene of mitochondrial cytochorome c oxidase subunit I (COI) are included.

Molecular Identification and Morphological Development of Larvae of Psettina tosana Collected from Southern Sea of Korea (한국 남해에서 채집된 사량넙치 Psettina tosana 자어의 분자 동정 및 형태 발달)

  • Ji, Jae-Min;Yu, Hyo-Jae;Hwang, Kang-Seok;Park, Jeong-Ho;Lee, Jeong-Hoon;Kim, Jin-Koo
    • Korean Journal of Ichthyology
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    • v.29 no.4
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    • pp.244-251
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    • 2017
  • A total of 15 larvae [3.53~19.49 mm standard length (SL)] belonging to the family Bothidae collected from the southern sea of Korea in 2016 were identified as Psettina tosana based on 434 base-pair sequences of mitochondrial DNA cytochrome c oxidase subunit I. Larvae of Psettina tosana have anterior-most two elongated dorsal fin rays. Uniserial melanophores present on the dorsal and anal fin base, whereas melanophores on the body absent. An inflection point in the relative growth of head length and head depth against SL was shown between 9.93 mm and 10.73 mm SL. The examined larvae of Psettina tosana are clearly distinguished from the most similar species, Psettina iijimae in having no melanophore patches in the proximity of dorsal and anal fin base.

Occurrence of Eggs and Larvae of Blackfin Flounder Glyptocephalus stelleri (Pleuronectidae, Pisces) off Wangdol-cho, East Sea (동해 왕돌초 주변해역에서 기름가자미 Glyptocephalus stelleri (가자미과, 어상강) 어란과 자어 출현)

  • Lee, Hae Won;Lee, Soo Jeong;Yang, Jae Hyung;Lee, Jae Bong;Cha, Hyung Kee;Kim, Jin-Koo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.47 no.5
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    • pp.654-658
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    • 2014
  • We report the first identification of a spawning ground of the blackfin flounder Glyptocephlaus stelleri near the Wangdol-cho sea mountains, located in the southern East Sea. Eggs and larval fish of G. stelleri were collected during April and June, 2014, when an abundance of eggs was found in the southern area of Wangdol-cho. Our findings suggest that G. stelleri prefers to spawn in the vicinity of the off-shore sea mountains, where the temperature is between 10 and $12^{\circ}C$ and the water depth is 100 m, rather than inshore.

New Record of the Schooling Bannerfish Heniochus diphreutes (Perciformes: Chaetodontidae) from Pohang, Korea (한국 포항에서 채집된 나비고기과(Chaetodontidae) 어류 1 미기록종 Heniochus diphreutes의 최초 출현)

  • Lee, Yu-Jin;Kim, Jin-Koo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.54 no.6
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    • pp.1017-1022
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    • 2021
  • We collected a specimen of Heniochus diphreutes Jordan, 1903 belonging to the family Chaetodontidae on December 26, 2020 in Pohang-si, Gyeongsangbuk-do, using the set net. Although, H. diphreutes is morphologically very similar to Heniochus acuminatus, it can be distinguished from the latter based on the number of dorsal fin spines, mostly 12 in H. diphreutes vs 11 in H. acuminatus, and the percentage of snout length in head length, which is less than 12% in H. diphreutes vs more than 13% in H. acuminatus. Compared to H. acuminatus, H. diphreutes has a shorter snout, two-three rows of teeth, convex ventral profile of the head, more angular anal fins, and a black pattern extending to the longest soft rays on the posterior part of the anal fin. Molecular analyses showed that our specimen perfectly matched H. diphreutes based on mtDNA COI sequences data. We propose a new Korean name for H. diphreutes, "Jjal-beun-ib-du-dong-ga-li-dom".

First Record of Goatfish Upeneus subvittatus (Pisces: Mullidae) from Jeju Island, Korea (한국 제주도에서 채집된 촉수과(Mullidae) 어류 1첫기록종, Upeneus subvittatus)

  • Si-Won Choi;Se Hun Myoung;Jeong-Hoon Lee;Jung Nyun Kim
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.57 no.2
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    • pp.169-176
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    • 2024
  • On November 9, 2022, a goatfish (Mullidae) that had not been previously reported in Korea was collected during offshore fisheries resources research near Jeju Island. Based on the morphological identification, this goatfish was identified as the genus Upeneus owing to the presence of palatine teeth and vomerine teeth, as well as the proximal part of anterior part of second dorsal fin. Additionally, through molecular identification, the previously unreported goatfish was identified as U. subvittatus with a 99.8% match in the mtDNA COI region. Goatfish U. subvittatus has no patterns on its body and dark bands on both the lower and upper caudal fins, making it well distinguishable from the four species of genus Upeneus reported in Korea. U. vittatus, reported in Japan, showed morphological differences from U. subvittatus in that the dark band on the lower lobe of the caudal fin was wider, and longitudinal stripes were present on the body. Based on the morphological characteristics of U. subvittatus, we suggest a new Korean name, "Jul-mu-nui-kko-li-chog-su".

On Schmarda's lost earthworm and some newly found New Zealand species (Oligochaeta: Megadrilacea: Lumbricidae, Acanthodrilidae, Octochaetidae, & Megascolecidae s. stricto)

  • Blakemore, Robert J.
    • Journal of Species Research
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    • v.1 no.2
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    • pp.105-132
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    • 2012
  • The saga of Megascolides orthostichon (Schmarda, 1861)-the first native worm described from Australasia-continues as its type-locality is unequivocally returned from Hobart, Tasmania to Mt Wellington, Auckland where a brief survey failed to unearth it. Since it has not been seen for 150 yrs, it may qualify under NZTCS or IUCN classification as 'Nationally Critical' if not 'Extinct'. New reports are for exotic Megascolecidae Anisochaeta kiwi sp. nov. and A. kiwi mihi sub-sp. nov. plus addition to the NZ faunal list of Australian Anisochaeta macleayi (Fletcher, 1889) that, due to its wide distribution in Australia and now New Zealand, may be a candidate model-species suitably resilient for eco-toxicological culture and monitoring. For holarctic Lumbricidae, new records are of Dendrobaena attemsi (Michaelsen, 1903) and the Murchieona muldali (Omodeo, 1956) morph or subspecies of M. minuscula (Rosa, 1906), neither lumbricid previously uncovered in Asia/Australasia. Also found for the first time outside its East Asian homeland is Eisenia japonica (Michaelsen, 1892) (which is compared to Japanese E. japonica hiramoto sub-sp. nov. and to E. anzac Blakemore, 2011). Records of these exotics plus recent new native species described by the author-including two, Rhododrilus mangamingi and Deinodrilus orcus spp. novae, herein-raise the numbers of megadriles known from New Zealand to 228 (sub-)species in five families. Preliminary mtDNA COI sequence barcodes are presented. Genus Tokea Benham, 1904 is revived on its lack of dorsal pores, losing or gaining some species with Megascolides M'Coy, 1878. An updated checklist of all 228 New Zealand taxa is appended.

First Record of the Flower Cardinalfish, Ostorhinchus fleurieu (Apogonidae) Collected from Jejudo Island, Korea (한국 제주도에서 채집된 동갈돔과 어류, Ostorhinchus fleurieu 첫 기록)

  • Kang-Hyun Lee;Sun-Chan Ahn;Jin-Koo Kim
    • Korean Journal of Ichthyology
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    • v.36 no.2
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    • pp.193-198
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    • 2024
  • Two specimens of Ostorhinchus fleurieu (54.25 mm, 55.64 mm SL) were collected by angling for the first time from Seogwipo-si, Jejudo Island, Korea on September and November 2023. This species is readily distinguished from the congeneric species, O. aureus by the number of total gill rakers (19~23 in O. fleurieu vs 22~27 in O. aureus) and shape of dark stripe on caudal peduncle (poorly defined, barrel shaped in O. fleurieu vs. well-defined, hourglass shaped in O. aureus). A total of 560 base pairs of mitochondrial DNA cytochrome c oxidase subunit I region of our two apogonid individuals perfectly matched with those of O. fleurieu (MT076481) registered in NCBI. Here, we propose the new Korean name "Kkoch-dong-gal-dom" for the species O. fleurieu.