• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2005년도 제5회 발생공학 국제심포지엄 및 학술대회
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• pp.46-46
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• 2005

• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2005년도 제5회 발생공학 국제심포지엄 및 학술대회
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• pp.46-46
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• 2005

• BMB Reports
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• 제41권9호
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• pp.664-669
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• 2008

Znf230, the mouse homologue of the human spermatogenesis-related gene, ZNF230, has been cloned by rapid amplification of cDNA ends (RACE). This gene is expressed predominantly in testis, but its expression in different testicular cells and spermatogenic stages has not been previously analyzed in detail. In the present study, the cellular localization of the Znf230 protein in mouse testis and epididymal spermatozoa was determined by RT-PCR, immunoblotting, immunohistochemistry and immunofluorescence. It is primarily expressed in the nuclei of spermatogonia and subsequently in the acrosome system and the entire tail of developing spermatids and spermatozoa. The results indicate that Znf230 may play an important role in mouse spermatogenesis, including spermatogenic cell proliferation and sperm maturation, as well as motility and fertilization.

• 한국발생생물학회지:발생과생식
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• 제4권1호
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• pp.37-43
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• 2000

생쥐 정소에서 밀착결합단백질의 일종인 zonular occludens-1 (ZO-1)의 발현을 조사하였다. RT-PCR결과 ZO-1의 2가지 isoform인 ZO-1$\alpha$＋, ZO-1$\alpha$-의 발현을 확인하였다. 생쥐 신생 및 성체의 정소에서 분자량 225 및 2001 KDa의 2종의 ZO-1의 단백질항원의 발현을 확인되어 RT-PCR의 결과와 일치하였다. ZO-l$\alpha$＋에 대한 ZO-l$\alpha$-의 상대적 발현량은 성숙에 따라 증가하였다. 2종의 ZO-1항원을 동시에 인식하는 항체를 사용한 면역염색을 통해 세정관 외곽의 Sertoli세포 사이의 접촉부위 및 Sertoli 세포와 생식세포 접촉부위에서 ZO-1의 존재를 확인하였다. ZO-1은 세정관내 세포들 사이의 결합부위 및 세포질에서 공통적으로 발현되지만 성숙에 따라 Sertoli 세포의 결합부위에서 강한 신호가 검출되었다. 2종의 ZO-1 항원의 상대적 발현량의 변화 및 세정관 외곽의 분포의 강화는 기능적 혈액정소장벽의 출현 및 정자형성의 진행과 관련된 것으로 사료된다.

• Animal cells and systems
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• 제13권2호
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• pp.205-212
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• 2009

Known as a female hormone, estrogen, performs important functions, and the activities of the hormone are mediated via the estrogen receptor. The principal objective of the present study was to assess the effects of a estrogen receptor agonist in male reproductive organs. In this study, the estrogen receptor alpha agonist, PPT, was injected subcutaneously into adult male mice. The effects of PPT on the murine reproductive system were histologically assessed at 3,5, and 8 weeks after treatment. In the treatment group, reductions were observed in the weight of the body, testis and epididymis. Microscopic examination revealed a reduction in seminiferous tubular diameter in the testis, and epithelial cell height in the epididymis during the experiment. 8 weeks after treatment, spermatogenesis was not detected, nor was the lumen of the seminiferous tubules. In the fertility test, 1 week after PPT injection, the fertilizing ability of males was decreased, and on the 2nd and 3rd weeks, complete infertility was observed. In conclusion, the injection of high concentrations of PPT into adult males induced physiological changes, including infertility, and also induced morphological changes, including a reduction in the height of epithelial cells within the reproductive system.

• BMB Reports
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• 제38권6호
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• pp.739-747
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• 2005

Full-length cDNA sequences of four novel SPATA4 genes in chimpanzee, cow, chicken and ascidian were identified by bioinformatic analysis using mouse or human SPATA4 cDNA fragment as electronic probe. All these genes have 6 exons and have similar protein molecular weight and do not localize in sex chromosome. The mouse SPATA4 sequence is identified as significantly changed in cryptorchidism, which shares no significant homology with any known protein in swissprot databases except for the homologous genes in various vertebrates. Our searching results showed that all SPATA4 proteins have a putative conserved domain DUF1042. The percentages of putative SPATA4 protein sequence identity ranging from 30% to 99%. The high similarity was also found in 1 kb promoter regions of human, mouse and rat SPATA4 gene. The similarities of the sequences upstream of SPATA4 promoter also have a high proportion. The results of searching SymAtlas (http://symatlas.gnf.org/SymAtlas/) showed that human SPATA4 has a high expression in testis, especially in testis interstitial, leydig cell, seminiferous tubule and germ cell. Mouse SPATA4 was observed exclusively in adult mouse testis and almost no signal was detected in other tissues. The pI values of the protein are negative, ranging from 9.44 to 10.15. The subcellular location of the protein is usually in the nucleus. And the signal peptide possibilities for SPATA4 are always zero. Using the SNPs data in NCBI, we found 33 SNPs in human SPATA4 gene genomic DNA region, with the distribution of 29 SNPs in the introns. CpG island searching gives the data about CpG island, which shows that the regions of the CpG island have a high similarity with each other, though the length of the CpG island is different from each other.This research is a fundamental work in the fields of the bioinformational analysis, and also put forward a new way for the bioinformatic analysis of other genes.

• 한국동물학회지
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• 제32권3호
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• pp.264-274
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• 1989

본 실험은 생쥐에서 부정소액내의 단백질 성분과 조성에 미치는 정소액과 정자의 영향을 알아보기 위하여, 성적 성숙시기에 따라 정소와 부정소의 조직분화 양상을 관찰하였으며, 강소분화 특징에 따라 체취한 부정소액은 전기영동법으로 단백질을 분석하여 다음과 같은 결과를 얻었다. 정소와 부정소는 생후 10일군에서 미분화 상태였고, 20일군에서 세정관의 강소는 형성되지 않았으나 부정소는 두부에서 미부에까지 강소가 형성되었으며, 35일군에서는 정소내 세정관의 강소가 형성되었고 정세포는 정자로 분화되었고, 부정소의 상피세포는 principal cell과 clear cell로 분화되었으나 부정소로 유입된 정자는 없었다. 80일군에서는 정소와 부정소가 완전히 본화되었고 부정소로 유입된 많은정자가 관찰되었다. 그리고 부정소액의 전기영동상에는 혈청내의 성분과 다른 단백질이 모두 28종이 나타났는데, 그 중 12종은 부정소액에만 존재하는 부정소 특이단백질이었고, 16종은 정소액에도 공통으로 존재하는 단백질이었다. 또한 이 단백질들은 성숙시기에 따라 종류가 다르게 나타났으며, 성체에서 나타난 3종의 단백질은 부정소의 부위에 따라 양적인 변화를 나타냈다. 이상의 결과로 보아 부정소액내 단백질의 성분과 조성은 정자를 포함한 정소액의 유입과 부정소 상피세포의 분비 및 흡수의 조절작용에 의하여 변화되는 것으로 사료된다. 따라서 부정소액내의 TEP와 ESP는 부정소 정자의 성숙에 어떤 중요한 역할을 할 것으로 사료된다.

• 동의생리병리학회지
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• 제28권4호
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• pp.384-389
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• 2014

The purpose of this study is to investigate the modulatory effect of oroxylin A on hydrogen peroxide production in TM4 mouse testis sertoli cells induced by the synthetic analog of double-stranded RNA [polyinosinic-polycytidylic acid]. Hydrogen peroxide production was measured by dihydrorhodamine 123 (DHR) assay. Oroxylin A significantly inhibited the polyinosinic-polycytidylic acid (PIC)-induced production of hydrogen peroxide for 0.5, 2, 12, 18, and 24 hr incubation at the concentrations of 5, 10, 25, and $50{\mu}M$ in TM4 (P < 0.05) in dose dependent manner. These results suggest that oroxylin A has a protective effect against PIC-induced cellular toxicity with its inhibition of hydrogen peroxide production in PIC-induced sertoli cells.

• 대한생식의학회:학술대회논문집
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• 대한불임학회 2006년도 The 5th Biannual Meeting of Pacific Rim Society for Fertility and Sterility
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• pp.168.1-168.1
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• 2006

• Parasites, Hosts and Diseases
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• 제44권2호
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• pp.167-169
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• 2006

After infection of male mice with the plerocercoids (spargana) of Spirometra mansoni, serum levels of estrogen and testicular weight were analyzed by enzyme-linked immunosorbent assay (ELISA) and weighing machine, respectively. The serum level of estrogen increased progressively in infected mice compared with normal controls, whereas the testicular weight of infected mice decreased significantly (P < 0.05). These results suggest that certain substances from spargana change the steroid hormone metabolisms in the host by unknown pathways, and chronic infection may contribute to change of the function of steroid hormone target organ, i.e., testis, in male mice.