• Title/Summary/Keyword: mouse skin

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Hair Growth Promotion Effect of a Bio-Active Shampoo, Bonogen in C57BL/6 Mice (C57BL/6 마우스에서 기능성 샴푸 Bonogen의 양모 촉진 효과)

  • Hong, Jin-Tae;Lee, Se-Ra;Kim, Hwan-Hee;Jo, Young-Kwang;Baek, In-Jeoung;Yon, Jung-Min;Nahm, Sang-Seop;Kwack, Dong-Hoon;Lee, Jung-Eun;Lee, Beom-Jun;Yun, Young-Won;Kim, Cheol-Jung;Nam, Sang-Yoon
    • Toxicological Research
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    • v.22 no.3
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    • pp.221-228
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    • 2006
  • Bonogen shampoo is composed of several plant extracts which are known to be used in oriental medicine. This study was carried out to investigate the effects of Bonogen shampoo on hair growth in an alopecia model of C57BL/6 mice. There were eight male and female experimental groups including distilled water(DW: negative control), a commercial shampoo[M], 3% minoxidil (MXD) and Bonogen shampoo(BNG). Dorsal skin hair of six-week-old mice was trimmed with an electric clipper carefully not to damage the skin. The next day, mice without skin scratch were selected, randomized and separated in 10 mice per group. The test compounds were topically treated with 0.15 ml per mouse or dorsal skin for 21 days daily and then washed thoroughly with DW. The hair regrowth was determined photographically at 0, 4, 7, 10, 15, 18, and 21 days and histologically at day 21. No clinical signs were observed in all mice. Although body weight was slightly increased in 3% MXD group than other groups, it was not significant. Hair regrowth began to be promoted after 14 days and appeared a distinct regrowth pattern in all animals by topical treatment of test compounds at 18 days. In particular, the topical treatment of bonogen shampoo or 3% MXD for 21 days to dorsal skin accelerated hair regrowth faster than DW or M shampoo. At 21 days, the hair regrowth promotion speed was in order of 3% MXD>BNG>M>DW. The bonogen shampoo or 3% MXD also promoted hair follicle elongation compared to the negative control. These results suggest that bonogen shampoo has hair growth promoting activities and may be useful for treatment of bald or alopecia.

Effect of Kami-chungsimyeunjatang on atopic dermatitis-like skin lesions induced in NC/Nga mice by mite antigen stimulation (가미청심연자탕(加味淸心蓮子場)이 NC/Nga mice의 아토피양(樣) 피부염에 미치는 영향)

  • Han, Jae-Kyung;Kim, Yun-Hee;Yoon, Ji-Yeon
    • The Journal of Pediatrics of Korean Medicine
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    • v.21 no.1
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    • pp.87-116
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    • 2007
  • Objectives : The purpose of this study is to examine of the effect of Kami-chungsimyeunjatang(KCSYJT) medicine on the atopy eruption control. Methods : The expression of IgE, IL-4, IL-6, $TNF-{\alpha}$, IgG2b, IgM, IgG2a and IgG1 level in serum, and $IFN-{\gamma}$ production by KCSYJT were analyzed. CD3e+/CD69+, CD4+/CD25+, B220+/IgE+ and B220+/CD23+ positive cells by flow cytometry in splenocytes were assayed and the revelation of CD3e+/CD69+, CD4+/CD8+ and CD4+/CD25+ marker in PBMC, spleen and DLN were observed. The outturn of IL-4, eotaxin 2, CCR3, TARC mRNA in splenocytes werw observed. We also analyzed NC/Nga mice's ear, DLN and neck-back skin after biopy and dye by H&E, and toluidine staining (mast cells marker) method, measured about epidermis and dermis part in comparison with control group. Results : NC/Nga mice suffered from dermatitis very similar to human AD with IgE hyperproduction. Specially, result that measure IgE content in serum on 8 weeks, 12 weeks, 16 weeks decreased remarkably than control group. After experiment end, result that observe revelation CD3e+/CD69+, CD4+/CD8+ and CD4+/CD25+ marker in PBMC, spleen and DLN establishment observed recover as normal with political background. And decreased than result control group which measure IL-4, IL-6, $TNF-{\alpha}$, IgG2b, IgM, IgG2a, IgG1 level in serum, and $IFN-{\gamma}$ production secreted in Th1 cell displayed increase by KCSYJT medicines. Ear thickness decrease than control group in result that observe effect that get in ear of a NC/Nga mouse. Course inflammation immunocyte etc.. permeated of result that effect that KCSYJT medicines get to NC/Nga mouse's skin establishment analyzes ear, DLN and neck-back skin after biopy, and dye by H&E, and toluidine staining (mast cells marker) method decreased about epidermis. and inflammation of dermis part remarkably than control group. Immunohistochemical examination of the skin lesion showed decrease by KCSYJT medicines on numbers of mast cells (CCR3) and CD4+ T cells containing IL-4 necessary for IgE. Conclusions : Th1 cell and Th2 cell was observed to be shift by secretion amount of IL-4 and $IFN-{\gamma}$ by KCSYJT medicines. Therefore, the KCSYJT medicine turned out to be useful in allergy autoimmune disease.

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Effect of High Fat Diet and Calorie-restricted Diet on Protein Kinase C Activity in Mouse Epidermal Cell (고지방식이와 열량제한식이가 백서상피세포의 Protein Kinase C 활성에 미치는 영향)

  • Choe, Myeon
    • Journal of Nutrition and Health
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    • v.24 no.3
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    • pp.149-156
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    • 1991
  • To determine the effect of dietary fat and calorie level on protein kinase C(PKC) activity in mouse epidermal cells, female BALB/C mice (4weeks of age) were placed on high (24.6% ), moderate(5%) fat or calorie-restricted diets for at least 4 weeks. Diets were formulated on a nutrient/kcal basis such that the mice consumed the same amounts of protein. vitamins, minerals and fiber per kcal. PKC was assayed by the procedure of Wise et at. An apparent increase of PKC activity was observed from the aminal fed high fat diet when compared with the aminal fed moderate fat diet. PKC activity was decreased 40% by calorie restriction. In summary levels of dietary fat may contribute to mechanism of tumor promotion by increasing PKC activity in the mouse skin model.

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The Change of ATPase-positive Dendritic Cell and the Effect of Green Tea in Mouse Skin by Ultraviolet B Irradiation (자외선 B 조사에 의한 마우스 피부 ATPase 양성 가지세포의 변화 및 녹차투여의 효과)

  • Kim Sung-Ho;Kim Se-Ra;Lee Hae-June;Lee Jin-Hee;Kim Yu-Jin;Kim Jong-Choon;Jang Jong-Sik;Jo Sung-Kee
    • Toxicological Research
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    • v.20 no.4
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    • pp.315-320
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    • 2004
  • In this study we assessed the influences of ultraviolet (UV) light B radiation on epidermal ATPase-positive dendritic cell (DC) and the effect of green tea treatment in ICR mouse. The extent of changes following 200 mJ/$cm^2$ (0.5 mW/sec) was studied at 0, 6, 12, 18, 24, 30 or 36 hours after exposure. SBCs were decreased by 6 hours after irradiation. There was tendency to decrease from 6 hours to 24 hours and had little further change from then to 36 hours after irradiation. The mice that received 0, 50, 100, 200, 300 or 400 mJ/$cm^2$ of UVB were examined 24 hours after irradiation. The DCs were decreased as the radiation dose increases from 100 to 400 mJ/$cm^2$. The frequency of UVB (200 mJ/$cm^2$)-induced DC decrease was reduced by treatment of green tea (i.p. and topical application, p<0.01).

Properties and Activitiy Screening of Chromone Derivatives (크로몬 유도체의 물성 및 활성검색)

  • 김영로;이상현;김경순;정춘식;정재훈;김박광
    • YAKHAK HOEJI
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    • v.44 no.2
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    • pp.107-114
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    • 2000
  • We have synthesized 4-isonitroso-4H-1-benzopyran and 4-amino-2,3-dihydro -4H-1-benzopyran of chromone derivatives by using condensation method. Physico-chemical properties of these compounds were measured and analyzed by UV and HPLC method. The correlation coefficient of their methanol solutions by UV were 0.9992 and 0.9994, respectively. And oxime compound was resolved within 4 min and had a detection limit of 3 ng at S/N=3 by HPLC using a reversed phase column with three solvents(MeOH, $H_2O$, HAc). The amino compound was resolved within 4.5 min and had a detection limit of 10 ng at S/N=3 by HPLC under the same conditions. Anti-diabetic effect of chromone derivatives were investigated in the streptozotocin (STZ)-induced diabetic rats. Diabetes was induced in male Sprague-Dawley rats by injections of STZ (45 mg/kg, i.v). The investigation of the hair growth effect of isonitrosobenzopyran and 4-aminobenzopyran on the hair of black mouse (C57BL/6) was also carried out. The administraion of their ethanol solution to the black mouse (C57BU/6) through skin them promoted the growth of hair.

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Mutagenecity Test of SDK (SDK시제품(가칭)에 대한 변이원성시험)

  • 정지윤;이원우;임종희;남정석;제정환;이광훈;강병철;이병희;박재학
    • Toxicological Research
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    • v.14 no.2
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    • pp.211-216
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    • 1998
  • In order to evaluate the mutagenic potential of SDK(skin decontamination kit) produced by Agency for Defense Development(ADD), were performed Salmonella typhimurium reversion assay, chromosomal aberration test on chinese hamster ovarian cells and in vivo micronucleus assay using mouse bone marrow cells according to the established regulation of Korean Food and Drug Administration. In the reverse mutation test using Salmonella typhimurium TA98, TA100, TA1535 and TA1537 did not in-crease the number of revertant at any of the concentration tested in this study. SDK did not increase the number of cells having structural or numerical chromosome aberration in cytogenetic test. In mouse micronucleus test, no significant increase in the occurrence oj micro nucleated polychromatic erythrocytes were observed in ICR male mice intraperitoneally administered with SDK. These results indicate that SDK has no mutagenic effects under these experimental conditions.

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Effect of Hwangryunhaedoktang on Contact Hypersensitivity and Passive Cutaneous Hypersensitivity in Mice

  • Lee, Kun-Ho;Shin, Young-Wook;Kim, Dong-Hyung
    • Biomolecules & Therapeutics
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    • v.12 no.2
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    • pp.129-132
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    • 2004
  • During the screening program to discover antiatopic agents from herbal formulas, we investigated the inhibitory effect of Hwangryunhaedoktang (HT) on passive cutaneous anaphylaxis and oxazolone-induced mouse ear dermatitis. HT significantly inhibited PCA reaction in mice at doses of 50 and 200 mg/kg with inhibitory activity of 31 and 53%, respectively. HT at concentration of 0.05 and 0.1% inhibited ear swelling by 23 and 46% at 16 days in oxazolone-induced mouse ear dermatitis. Both HT with anti without human intestinal microflora showed potent inhibitory activity on the ${\beta}$-hexosaminidase release induced by IgE. Based on these findings, HT may be a usable agent for skin disorder contact dermatitis.

Development of Interface device with EOG Signal (EOG(Electro-oculogram) 신호를 이용한 Interface 장치 개발)

  • Kim, Su-Jong;Ryu, Ho-Sun;Kim, Young-Chol
    • Proceedings of the KIEE Conference
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    • 2006.07d
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    • pp.1821-1823
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    • 2006
  • This paper presents a development of interface device for electro-oculogram(EOG) signal and it's application to the wireless mouse of wearable PC. The interface device is composed of five bio-electrodes for detecting oculomotor motion, several band-pass filters, instrumentation amplifier and a microprocessor. we have first analyzed impedance characteristics between skin and a bio-electrode. since the impedance highly depends on human face, it's magnitude differs from person. this interface device was applied to develop a wireless mouse for wearable PC, as a Bio Machine Interface(BMI). Where in the prompt on PC monitor is controlled by only EOG signals. this system was implemented in a Head Mount Display(HMD) unit. experimental results show the accuracy of above 90%.

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Properties and Hair-growth Effect of Chrysin 7-O-crotonate (크라이신 7-O-크로토네이트의 물성 및 육모효과)

  • 신준수;김연희;정재훈;김양배;김박광
    • YAKHAK HOEJI
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    • v.43 no.3
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    • pp.316-319
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    • 1999
  • Chrysin 7-Ο-crotonate was synthesized by condensing crotonic acid with chrysin in organic solvent, tetrahydrofuran (THF) using dicyclohexylcarbodiimide (DCC) and 4-dimethylaminopyridine (DMAP). Its structure was indentified by NMR, MS, UV, IR etc. We also investigated the physico-chemical properties and set up the quantitative anytical method of this compound. The correlation coefficient of calibration curve measured at the isobestic point (340 nm) on this compound was approximately 0.9994 by absorption spetrophtometry. Detection limit was 1.6ng at S/N=3 by using a RP column by HPLC. The hair growth effect fo chrysin 7-Ο-crotonate on the hair of black mouse (C57BL/6), was carried out using paste method. When its ethanol solution was administered to this black mouse by route of skin, this compound promoted the growth of hair.

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Chronic Effects of Hair, Blood And Testis In Black Mouse With Neutron Irradiation By Lying Flat Pose

  • Chun, Ki-Jung;Yoo, Bo-Kyung;Kim, Bong-Hee
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.105.1-105.1
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    • 2003
  • The purpose of this study is to investigate the biological effects in black mouse by neutron irradiation at HANARO reactor in KAERI. Neutrons readily penetrate the charged field of an atomic nucleus because they are electrically neural. And so it can fight cancer with the radiation released when an atom of the element boron absorbs a neutron. The main patient in BNCT facility is brain cancer and sometimes skin cancer in foreign countries until now. Therefore, mice were laid flat and so irradiated at the direction of the front. (omitted)

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