• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.12
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• pp.6208-6214
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• 2012

The most essential but missing components to understand and use toxic substances from marine microalgae are developing the fast, easy and economical determining technology for detecting it. In this paper we produced the antibodies against saxitoxin (STX). Mariculture keyhole limpet hemocyanin (mcKLH) and ovalbumin (OVA) were used as carrier proteins. mcKLH-STX conjugates were injected into the peritonial cavity of BALB/c mouse for immunization. After bleeding from mouse, anti-STX antiserum was isolated. Indirect enzyme-linked immunosorbent assays (ELISA) was performed to determine antiserum titer using the microtiter plate coated with free STX and OVA-STX. A goat anti-mouse IgG-phosphatase conjugate was used as secondary antibody to enable chromogenic reaction. Reactions of anti-STX antiserum were very specific on the OVA-STX and free STX. Sensitivity of anti-STX antiserum on STX was very high and STX detection limit was to be 64.9 ng/kg for indirect ELISA.

• Proceedings of the IEEK Conference
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• 1999.06a
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• pp.516-519
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• 1999

Gaze detection is to find out the position on a monitor screen where a user is looking at, using the image processing and computer vision technology, We developed a computer interface system using the gaze detection technology, This system enables a user to control the computer system without using their hands. So this system will help the handicapped to use a computer and is also useful for the man whose hands are busy doing another job, especially in tasks in factory. For the practical use, command signal like mouse clicking is necessary and we used eye winking to give this command signal to the system.

• Proceedings of the Korean Biophysical Society Conference
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• 2002.06b
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• pp.47-47
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• 2002

Nucleoredoxin (NRX) is a 435-amino-acid redox protein with similarity to TRX but with a -Trp-Cys-Pro-Pro-Cys- catalytic site (instead of - Trp-Cys-Gly-Pro-Cys-). It has been cloned from a mouse YAC library and localized to the nucleus In this study, amino acid sequences of rat and human NRX were determined by RT-PCR and genomic PCR. (omitted)

• Reproductive and Developmental Biology
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• v.38 no.3
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• pp.99-105
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• 2014

In this study, to further understand the mechanism of animal growth and to develop a miniature transgenic animal model, we constructed and tested tetracycline-inducible RNAi system using shRNA targeting the mRNA of mouse insulin-like growth factor (mIGF-1) or mouse growth hormone receptor (mGHR) gene. Quantitative real-time PCR analysis of mouse liver cell (Hepa1c1c7) cells transfected with these vectors showed 85% or 90% of expression inhibition effect of IGF-1 or GHR, respectively. In ELISA analysis, the protein level of IGF-1 in the cells expressing the shRNA targeting IGF-1 mRNA was reduced to 26% of non-transformed control cells. Unexpectedly, in case of using shRNA targeting GHR, the IGF-1 protein level was decreased to 75% of control cells. Further experiments are needed to explain the lower interference effect of GHR shRNA in IGF-1 protein. Accumulated knowledge of this approach could be applicable to a variety of related biological area including gene function study, gene therapy, development of miniature animals, etc.

• 제어로봇시스템학회:학술대회논문집
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• 2001.10a
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• pp.138.2-138
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• 2001

This paper proposes the technique for recognizing the human´s emotion by using the CCD color image. To do this, we first acquire the color image from the CCD camera. And then propose the method for recognizing the expressing to be represented the structural correlation of man´s feature points(eyebrows, eye, nose, mouse), In the proposed method. Human´s emotion is divided into four emotion(surprise, anger, happiness, sadness). Finally, we have proven the effectiveness of the proposed method through the experimentation.

• Proceedings of the Zoological Society Korea Conference
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• 1996.10a
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• pp.173.1-173
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• 1996

No Abstract, See Full Text

• Proceedings of the Zoological Society Korea Conference
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• 2003.08a
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• pp.167.3-167
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• 2003

No Abstract, See Full Text

• Clean Technology
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• v.23 no.3
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• pp.248-255
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• 2017

In dismantling factories for recycling, it is important to input actual working data to a personal computer (PC) in order to monitor the work results and related recycling rate of the inputs. This should be performed with a keyboard, a mouse, or other devices. But when a worker is working in the factory, it could be bothersome or time consuming to go to the PC. Especially, workers who works at dismantling factories have a generally low education level are scared to use a PC, which could be used as a pretext for not using the PC. In some cases, data input is performed by a worker after the day's job. In this case, it could take additional time, the worker can make more mistakes, and the data could be unreliable. In this study, we developed a man-machine interface (MMI) device using a safety helmet. A joystick-like device, pushbuttons, and a radio frequency (RF) device for wireless communication is equipped in a safety helmet. This MMI device has functions similar to a PC mouse, and it has a long communication distance. RF is used because it consumes less battery power than Bluetooth. With this MMI device, workers need not go to a PC to input data or to control the PC, and they can control the PC from a long distance. The efficiency of PCs in a factory could be increased by using the developed MMI system, and workers at the dismantling factories could have less reluctance in using the PC.

• Journal of the Korean Society of Radiology
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• v.14 no.2
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• pp.157-168
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• 2020

Advancements in segmentation methodology has made automatic segmentation of brain structures using structural images accurate and consistent. One method of automatic segmentation, which involves registering atlas information from template space to subject space, requires a high quality atlas with accurate boundaries for consistent segmentation. The Allen Mouse Brain Atlas, which has been widely accepted as a high quality reference of the mouse brain, has been used in various segmentations and can provide accurate coordinates and boundaries of mouse brain structures for tractography. Through probabilistic tractography, diffusion tensor images can be used to map comprehensive neuronal network of white matter pathways of the brain. Comparisons between neural networks of mouse and human brains showed that various clinical tests on mouse models were able to simulate disease pathology of human brains, increasing the importance of clinical mouse brain studies. However, differences between brain size of human and mouse brain has made it difficult to achieve the necessary image quality for analysis and the conditions for sufficient image quality such as a long scan time makes using live samples unrealistic. In order to secure a mouse brain image with a sufficient scan time, an Ex-vivo experiment of a mouse brain was conducted for this study. Using FSL, a tool for analyzing tensor images, we proposed a semi-automated segmentation and tractography analysis pipeline of the mouse brain and applied it to various mouse models. Also, in order to determine the useful signal-to-noise ratio of the diffusion tensor image acquired for the tractography analysis, images with various excitation numbers were compared.

• Nutrition Research and Practice
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• v.2 no.2
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• pp.74-79
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• 2008

Zinc plays a protective role in anti-atherosclerosis but the clear mechanism has not been proposed yet. In the present study, we evaluated whether zinc modulates atherosclerotic markers, VACM-1 and ICAM-1 and cell viability both in endothelial cells in vitro and mouse aortic cell viability ex vivo. In study 1, as in vitro model, endothelial EA.hy926 cells were treated with $TNF{\alpha}$ for 5 hours for inducing oxidative stress, and then treated with Zn-adequacy ($15\;{\mu}M$ Zn) or Zn-deficiency ($0\;{\mu}M$ Zn) for 6 hours. Pro-atherosclerosis factors, VCAM-1 and ICAM-1 mRNA expression and cell viability was measured. In study 2, as ex vivo model, mouse aorta ring was used. Mourse aorta was removed and cut in ring then, cultured in a 96-well plate. Aortic ring was treated with various $TNF{\alpha}$ (0-30 mg/ml) and intracellular zinc chelator, N, N, N', N', -tetrakis (2-pyridylmethyl) ethylenediamine (TPEN, $0-30\;{\mu}M$) for cellular zinc depletion for 2 days and then cell viability was measured. The results showed that in in vitro study, Zn-adequate group induced more VCAM-1 & ICAM-1 mRNA expression than Zn-deficient group during 6-hour zinc treatment post-5 hour TNF-$\alpha$ treatment, unexpectedly. These results might be cautiously interpreted that zinc would biologically induce the early expression of anti-oxidative stress through the increased adhesion molecule expression for reducing atherosclerotic action, particularly under the present 6-hour zinc treatment. In ex vivo, mouse aortic ring cell viability was decreased as TNF-$\alpha$ and TPEN levels increased, which suggests that mouse aortic blood vessel cell viability was decreased, when oxidative stress increases and cellular zinc level decreases. Taken together, it can be suggested that zinc may have a protective role in anti-atherosclerosis by cell viability in endothelial cells and aorta tissue. Further study is needed to clarify how pro-atherosclerosis molecule expression is modulated by zinc.