• 웰빙융합연구
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• 제3권3호
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• pp.15-19
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• 2020

Consumers exposed to aesthetic brands can not only experience sensory pleasure but also lead to strong customer loyalty. Furthermore, aesthetic brands can expect to lead to the overall experience of the brand. They include the brand's sensory, emotional, behavioral and intellectual experience. Since aesthetic brands related to the sensory representation of customers, consumers exposed to aesthetic brands can expect to have a meaningful impact on the overall experience of the brand. The study will select aesthetic brands that distributed in Korea and then compare the aesthetic characteristics of each brand. It also seeks to verify the brand attractiveness, consistency, prominence and influence of brand motifs on the brand's sensual, emotional, behavioral and intellectual experience. To verify the influence between variables proposed in this study, we would like to conduct a multiple regression analysis. SAS 9.4 used to verify the hypotheses proposed in this study. Cronbach's α measure to verify reliability of each measurement item. In particular, brand attractiveness and brand motif can verify empirically to confirm that they play an important role in inducing brand experience.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2007년도 춘계학술발표대회
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• pp.10-13
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• 2007

모티프는 진화과정을 거치면서 단백질 서열상에서 부분적으로 높게 보존된 지역을 의미한다. 이러한 모티프는 단백질의 기능과 구조를 예측하거나 생물학적으로 관련성이 있는 단백질의 공통적인 특성을 기술하는데 사용된다. 또한, 모티프와 단백질 서열의 상관관계는 생물학적 기능 예측에 필수적이며, 이러한 예측 문제는 모티프 검색을 통해 서열에 존재하는 빈발한 서열패턴과 구조패턴을 통해 단백질 서열에 대한 분석이 가능하다. 이 논문에서는 단백질 서열에 존재하는 2차 구조 특성과 빈발패턴을 검색하고 추출된 정보를 이용하여 단백질 기능 분류에 활용하고자 한다.

• 복식문화연구
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• 제25권2호
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• pp.224-236
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• 2017

Kimono sleeves have continuously appeared in modern fashion as a design motif and this trend has become increasingly prevalent recently. However for research in the area of clothing composition for kimono sleeves in Korea, there has been a lack of development of master patterns based on basic data from the analysis of the standard body type of Korean people and finalized designs based on comparative experiments and analysis of pre-existing research Thus, there is no ideal master kimono sleeve pattern development research based on the examination of the results of the analysis of the standard body type of Korean people. Therefore by recognizing the importance of developing a master kimono sleeve pattern, this research aims to present a practical master kimono sleeve pattern for the standard body type of someone in their 30s. First, the four master patterns were collected and a dressing evaluation was conducted, and by selecting and improving one of these master designs, a final research master pattern was developed after a second dressing evaluation. Through this research, it was possible to design a master kimono sleeve pattern appropriate both externally and functionally for the standard body type of someone in their 30s, which has not been a consideration in previous research.

• 한국식물병리학회지
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• 제11권3호
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• pp.271-277
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• 1995

The RNA nucleotide sequences of the 3/-noncoding regions (3'-NCRs) of two Korean strains of turnip mosaic virus (TuMV), Ca and cqs, have been determined from their cDNA clones that encompassed the 3'-terminal regions of the viral genomic RNAs. The 3'-NCRs of both strains were 209 nucleotides long, terminated with GAC residues and poly (A) tails. The potential polyadenylational signal motif, UAUGU, was located 140 nucleotides upstream from the poly (A) tail in each of the virus. A highly conserved hexanucleotide sequence [A G U G A/U G/C], which was common in the 3'-NCRs of the potyvirus RNAs, was also found at the regions of 119 bases upstream from the 3'-end. Comparison of the 3'-NCRs of the two Korean isolates with those of four strains from Canada, China and Japan showed significantly identical genotypes (94.3∼99.5%). The secondary structure of three loops with long stems was found within the 3'-NCRs by sequence analysis. The substituted bases in the region among the six TuMV strains did not alter their secondary structures. Length of the 3'-NCRs of the know 11 potyviral RNAs and TuMV RNAs was different from one another and their nucleotide sequences showed 55.7% to 24.0% of homology. The 3'-NCR, therefore, is considered to be useful for phylogenetic studies in potyviruses.

• 한국실내디자인학회논문집
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• 제20권3호
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• pp.22-32
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• 2011

The purpose of this study is on the application of the facade design of k-national university in deagu. The subjects of the study examined 75 buildings from the 117 university buildings in main campus. The analysis items is analyzed by several elements; flat and sloping roof, main entrance, design principle, style of architecture, finishing materials, and characteristics of design surveyed by analysis items. The results are as follows: In the 1950s, 2 subjects have shown conflicting characteristics except brick finishing materials. In he 1960s, flat make up 75% of a whole, centrality in main entrances occur, but have an asymmetrical, during this period vertical motif have been underlined by repetition of column, stone has application to main adminstration building. The buildings in the 1970s were so characteristics of modern architecture by painting and flat except main entrance at the center of facade. In the 1980s, position of main entrance have a corner of a wall, finishing materials of a wall were beginning to use stone, glass and metal. The buildings in the 1990s and 2000s are looking a three-dimensional composition by rhythm of superimposition and segmentation and all finishing materials of analysis items are in use.

• Asian Pacific Journal of Cancer Prevention
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• 제13권8호
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• pp.3681-3685
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• 2012

Background: In breast cancer, estrogen receptors have been demonstrated to interact with transcription factors to regulate target gene expression. However, high-throughput identification of the transcription regulation relationship between transcription factors and their target genes in response to estradiol is still in its infancy. Purpose: Thus, the objective of our study was to interpret the transcription regulation network of MCF7 breast cancer cells exposed to estradiol. Methods: In this work, GSE11352 microarray data were used to identify differentially expressed genes (DEGs). Results: Our results showed that the MYB (v-myb myeloblastosis viral oncogene homolog [avian]), PGR (progesterone receptor), and MYC (v-myc myelocytomatosis viral oncogene homolog [avian]) were hub nodes in our transcriptome network, which may interact with ER and, in turn, regulate target gene expression. MYB can up-regulate MCM3 (minichromosome maintenance 3) and MCM7 expression; PGR can suppress BCL2 (B-cell lymphoma 2) expression; MYC can inhibit TGFB2 (transforming growth factor, beta 2) expression. These genes are associated with breast cancer progression via cell cycling and the $TGF{\beta}$ signaling pathway. Conclusion: Analysis of transcriptional regulation may provide a better understanding of molecular mechanisms and clues to potential therapeutic targets in the treatment of breast cancer.

• 한국자원식물학회지
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• 제22권3호
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• pp.273-280
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• 2009

A cDNA clone encoding a MDR-like ABC transporter protein was isolated from Brassica rapa seedlings, through rapid amplification of cDNA ends (RACE). This gene (named as Brmdr 1; GenBank accession no.: DQ296184 ) had a total length of 4222 bp with an open reading frame of 3900 bp, and encoded a predicted polypeptide of 1300 amino acids with a molecular weight of 143.1 kDa. The BrMDR1 protein shared 71.0, 62.5, 60.0 and 58.2% identity with other MDR proteins isolated from Arabidopsis thaliana (AAN28720), Coptis japonica (CjMDR), Gossypium hirsutum (GhMDR) and Triticum aestivum (TaMDR) at amino acid level, respectively. Southern blot analysis showed that Brmdr1 was a low-copy gene. Expression pattern analysis revealed that Brmdr1 constitutively expressed in the root, stem petals and stamens, but with lower expression in leaves and open flowers. The domains analysis showed that BrMDR1 protein possessed two transmembrane domains (TMDs) and two nucleotide binding domains (NBDs) arranging in "TMD1-NBD1-TMD2-NBD2" direction, which is consistent with other MDR transporters. Within NBDs three characteristic motifs common to all ABC transporters, "Walker A", "Walker B" and C motif, were found. These results indicate that BrMDR1 is a MDR-like ABC transporter protein that may be involved in the transport and accumulation of secondary metabolites.

• The Plant Pathology Journal
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• 제23권4호
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• pp.245-250
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• 2007

The complete genome sequence of Zucchini yellow mosaic virus stain A (ZYMV-A) isolated from a hollyhock (Althaea rosea) was determined by using RT-PCR with a series of primer sets. The virus genome consisted of 9593 nucleotides (nt), excluding the poly(A) tract at 3' terminus of the virus genome, with 5' and 3' untranslated region of 139 and 211 nt, respectively. The deduced polyprotein of ZYMV-A consisted of 3080 amino acid (aa) residues and was 351 kDa in molecular weight. All proteolytic cleavage sites of the polyprotein of ZYMV-A were compared with those of ZYMV strains, which showed the cleavage sites were conserved among ZYMV strains. The HC-Pro contained the KITC and PTK motifs, and the DAG motif was located at CP ORF of ZYMV-A, suggesting that ZYMV-A is aphid-transmissible. Phylogenetic tree analysis based on the complete genome among ZYMV strains or CP ORFs with other potyviruses showed ZYMV strains formed a distinct group. These results clearly confirmed that ZYMV-A was another distinct strain in ZYMV population at molecular level.

• 헤리티지:역사와 과학
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• 제52권4호
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• pp.18-37
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• 2019

국립중앙박물관의 외규장각 의궤 중 운보문단(雲寶紋緞) 책의(冊衣)를 대상으로 운보문의 편년 설정 방법을 도출한 결과는 다음과 같다. 첫째, 외규장각 의궤 중 운보문단 책의는 현종 즉위년(1659) 『효종국장도감의궤(상·하)』부터 영조 20년(1744) 『사도세자가례도감의궤』까지 86년간 89책(冊)에서 사용되었다. 둘째, 의궤 책의에 사용된 보문 종류는 14종으로 파악되었는데 잡보(雜寶)인 금정(金錠), 만자(卍字), 방승(方勝), 방승(쌍), 보주(寶珠), 산호(珊瑚), 서각(犀角), 서보(書寶), 애엽(艾葉), 여의두(如意頭), 전보(錢寶), 전보(쌍), 특경(特磬), 화보(畵寶)이다. 셋째, 운보문 판독 방법은 다음과 같다. (1)보문의 명칭을 결정한다. (2)운두(雲頭) 꼭지점을 기준으로 운두가 일방향(一方向)으로 배열되었는지 양방향(兩方向)으로 배열되었는지를 확인한다. (3)운두에 연결된 만자꼬리 중 상하꼬리 방향이 좌향인지 우향인지 파악한다. (4)운두를 중심으로 사분면에 배열된 보문 조합이 '1조(組) 보문형'인지 '2조 보문형'인지 확인하고 운두 꼭지점을 기준으로 좌향꼬리는 좌상(左上) 면에서 시계 역방향으로 보문을 판독하고 우향꼬리 운보문은 우상(右上) 면에서 시계 순방향으로 보문을 판독한다. 2조 보문인 경우는 첫 보문의 명칭을 가나다 순으로 따져 '제1조 보문'과 '제2조 보문'을 결정한다. (4)운보문에서 판독한 내용을 유물명부터 유물번호, 소장처, 왕조, 연도, 1-1면, 1-2면, 1-3면, 1-4면, 방향, 2-1면, 2-2면, 2-3면, 2-4면, 운두 크기, 단위문 크기 등 16항목으로 정리한다. 이러한 판독법의 활용을 용이하게 하기 위하여 운보문의 연대 파악을 위한 '정하 운보문 판독법'으로 명명하였다. 넷째, 현종 즉위년(1659)부터 영조 20년(1744)까지 86년간의 의궤 89책 책의에서 28종의 운보문단을 확인하였다. 그리고 운보문에 사용된 보문은 단독보문과 조합보문을 포함하여 45종으로 확인되었는데, 특히 보주(85건), 산호(84건), 서보(42건), 방승(쌍)(41건) 등이 많이 사용되었음을 알 수 있었다. 다섯째, 운보문 판독법을 연대가 정확한 초상화와 출토복식, 전세유물 등에 운보문 판독법을 적용해본 결과, 유물의 연대 파악에 도움이 될 수 있음을 확인하였다.

• Journal of Microbiology and Biotechnology
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• 제28권9호
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• pp.1547-1553
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• 2018

Hyaluronidases are a family of enzymes that catalyse the breakdown of hyaluronic acid, which is abundant in the extracellular matrix and cumulus oocyte complex. To investigate the activity of recombinant bovine sperm hyaluronidase 1 (SPAM1) and determine the effect of the Asn-X-Ser/Thr motif on its activity, the bovine SPAM1 open reading frame was cloned into the mammalian expression vector pCXN2 and then transfected to the HEK293 cell line. Expression of recombinant bovine hyaluronidase was estimated using a hyaluronidase activity assay with gel electrophoresis. Recombinant hyaluronidase could resolve highly polymeric hyaluronic acid and also caused dispersal of the cumulus cell layer. Comparative analysis with respect to enzyme activity was carried out for the glycosylated and deglycosylated bovine sperm hyaluronidase by N-glycosidase F treatment. Finally, mutagenesis analysis revealed that among the five potential N-linked glycosylation sites, only three contributed to significant inhibition of hyaluronic activity. Recombinant bovine SPAM1 has hyaluronan degradation and cumulus oocyte complex dispersion ability, and the N-linked oligosaccharides are important for enzyme activity, providing a foundation for the commercialization of hyaluronidase.