• Title/Summary/Keyword: mosaic-by

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The Study on an Advanced Algorithm for Auto-generation of MOSAIC Seam Lines

  • Park, Young-Hoon;Kim, Jin-Kwang;Kang, Young-Ku
    • Proceedings of the KSRS Conference
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    • 2003.11a
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    • pp.464-466
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    • 2003
  • In this paper an advanced algorithm for selecting a seam line automatically, which used to be selected by human operator for mosaicked images is presented. In addition to four factors proposed by automation theory, the FOM(Figure Of Merit) of tie point were taken into account to suggest the method to select a seam line applicatively and the algorithm was applied to mosaic test images.

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Characterization and RT-PCR Detection of Turnip Mosaic Virus Isolated from Chinese Cabbage in Korea (배추에서 분리한 순무 모자이크 바이러스의 특성 및 역전사 중합효소 연쇄반응법(RT-PCR)을 이용한 검정)

  • 박원목;최설란;김수중;최승국;류기현
    • Korean Journal Plant Pathology
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    • v.14 no.3
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    • pp.223-228
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    • 1998
  • Turnip mosaic virus)TuMV-Ca) was isolated from a Chinese cabbage showing severe mosaic and black necrotic spots symptoms in Korea. The virus was identified as a strain of TuMV by its host range test, particle morphology, serology, double stranded RNA analysis. For detection of the virus, reverse transcription and polymerase chain reaction(RT-PCR) was performed with a set of 18-mer TuMV-specific primers to amplify a 876 bp DNA fragment The virus was rapidly detected from total nucleic acids of virus infected tissues as well as native viral RNA of purified virion particles by RT-PCR. Detection limit of the viral RNA by RT-PCR was 10 fg.

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Mosaic disease of black locust(Robinia pseudoacacia L.) I. Symptom and transmission by grafting (아까시나무모자이크병에 관한 연구 I. 병징 및 접목전염)

  • Kim Chong Jin
    • Korean journal of applied entomology
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    • v.3
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    • pp.1-5
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    • 1964
  • Symptom of the mosaic disease of black locust which is presently widespread in Korea was observed and transmission of the disease was investigated by means of grafting Since its symptom was typically mosaic and its transmission was easily accomplished, the disease was considered to be caused by a virus. In investigation of transmission by grafting, it was found out that the discase was easily transmitted in both of the following cases, i. e., healthy seedlings grafted with diseased scions and diseased roots with healthy scions. In the healthy seedlings plus diseased scions grafting, some grafted seedlings were purposedly deprived of the diseased scions after, a certain length of time(10∼30 days), while some were left grafted. The former case showed much higher transmission rate than the latter case, but no fluctuation in rate of transmission by the length of contact period(10-30 days) was recognized. And even when the union between stock and diseased scion by callusing was not successful, the transmission was withnesed. On the basis of the symptom, transmission by grafting, and possibility of transmission by sap inoculation(not published yet), it is supposed that the mosaic virus belongs to the group of the black locust mosaic virus that has been reported in southeastern Europe by Atanasoff(1935) and Milinko et al(1961). It is very likely that the witches' broom of black locust recorded by Myung-O Kim et al(1961) in Korea is the mosaic disease.

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Effect of Cotton Leaf Mosaic Disease on Morphology, Yield and Fibre Characteristics of Upland Cotton in Pakistan

  • Akhtar, Khalid P.;Haq, M.A.;Ishaque, Wajid;Khan, M.K.R.;Khan, Azeem I.
    • The Plant Pathology Journal
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    • v.21 no.2
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    • pp.137-141
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    • 2005
  • The effect of cotton leaf mosaic disease on morphology, yield and fibre characteristics was examined for a susceptible cotton candidate variety CRIS-168. Plants inoculated at most susceptible growth stage (six week) under screen house showed severe mosaic symptoms. There was a significant reduction in plant height and yield. Cotton leaf mosaic disease was found to produce severe effects on plant morphology with 24.1% reduction in plant height, 25% in internode length and 37.5% in number of sympodia on main stem. However no changes were observed against number of monopodial branches per plant. Inoculated plants showed 82% decrease in yield/plant, 80% in number of boll set/ plant, 12.1% in boll weight, 12.8% in lint weight, 10.8% in seed weight, and 6.8% in seed index. Cotton leaf mosaic disease also showed effects on fibre characteristics with 0.8% decrease in GOT and 1.6% in fibre length. In contrast, uniformity ratio, fibre fineness and maturity index was increased by 20.5%, 14.4% and 0.9%, respectively.

Serological Detection of Plant Viruses with latex-test (Latex-Test에 의한 식물 바이러스의 검정)

  • 박은경;김정화;이영근
    • Journal of the Korean Society of Tobacco Science
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    • v.1 no.1
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    • pp.33-38
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    • 1979
  • A simple test using latex-agglutination was developed to detect serologically tobacco mosaic virus (TMV), soybean mosaic virus (SoyMV), and barley stripe mosaic virus (BSMV) from infected Plants. Latex spheres ( 0.81 $\mu$, Difco) were adsorbed with immuno globulin purified by electrophoresis from crude antiserum against viruses. The antibody- sensitized latex suspension was mixed with sap from virus -infected leaves in a glass capillary tube (inner diam. 1mm $\times$ 100 mm length) The mixture, after agitation, was observed under a stereo microscope at low magnification (X20 - X4O), to examine the reaction between antigen (Virus) and its antibody. Flocculation occurred when the reaction was positive.

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Characterization of a Korean Isolate of Dasheen mosaic virus Isolated from Taro (Colocasia esculenta Schott) in Korea

  • Kim, Min-Kyu;Kwon, Soon-Bae;Yoon, Ju-Yeon;Ryu, Ki-Hyun;Heo, Su-Jeong;Hong, Jeong-Ki;Kim, Kyung-Hee;Park, Jang-Kyung
    • The Plant Pathology Journal
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    • v.20 no.2
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    • pp.135-141
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    • 2004
  • A filamentous virus was isolated from taro (Colocasia esculenta Schott) showing mosaic and chlorotic feather-ing symptoms in Chuncheon, Gangwon province in 2002. Based on ELISA, its appearance in electron microscope, serological relationships, and RT-PCR using specific primer and nucleotide sequence analysis of the CP gene, the isolated virus was identified as Dasheen mosaic virus (DsMV) and designated as Korean isolated (DsMV-Kr). DsMV was not serologically related to Zantedeschia mosaic virus (ZaMV), which has been reported to infect an Araceae plants. Since the coat protein revealed electrophoretic heterogeneity, about 42 kDa, 39 kDa and 31 kDa by SDS-PAGE, an improved purification method was established for the production of antisera against DsMV-Kr. The purification method used in this study may be effectively applied to the purification of other filamentous viruses.

Separation of Heavy Metal Ions across Novel Mosaic Membrane (하전모자이크 막을 사용하여 중금속이온의 분리)

  • Song, Myung-Kwan;Lee, Jang-Oo;Yang, Wong-Kang
    • Proceedings of the Membrane Society of Korea Conference
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    • 2005.11a
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    • pp.96-101
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    • 2005
  • A theory for the material transports through ion exchange membrane has been developed on the basis of nonequilibrium thermodynamics by removing the assumption of solvent flow in the previous paper and applied to a detailed study of the ionic transport properties of new charged mosaic membrane(CMM) system. The CMM having two different fixed charges in the polymer membrane indicated unique selective transport behavior then ion-exchange membrane. The separation behavior of ion transport across the CMM with a parallel array of positive and negative functional charges were investigated. It was well-known the analysis of the volume flux and solute flux based on nonequilibrium thermodynamics. Our suggests preferential salt transport across the charged mosaic membranes. Transport properties of heavy metal ions, $Mg^{2+}$, $Mn^{2+}$and sucrose system across the charged mosaic membrane were estimated. As a result, we were known metal salts transport depended largely on the CMM. The reflection coefficient indicated the negative value that suggested preferential material transport and was independent of charged mosaic membrane thickness.

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Evaluation and Verification of Barley Genotypes with Known Genes for Resistance to Barley yellow mosaic virus and Barley mild mosaic virus Under Field Conditions in South Korea

  • Kim, Hong-Sik;Baek, Seong-Bum;Kim, Dea-Wook;Hwang, Jong-Jin;Kim, Si-Ju
    • The Plant Pathology Journal
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    • v.27 no.4
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    • pp.324-332
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    • 2011
  • Soil-borne barley yellow mosaic disease caused by Barley yellow mosaic virus (BaYMV) or Barley mild mosaic virus (BaMMV) gives a serious threat to the winter barley cultivated in the southern regions in Korea. It is important to develop resistant varieties for stable and high-yield production. The objectives of this study were to evaluate 22 genotypes of exotic barley germplasms carrying the resistance genes rym1 through rym12, with the exception of rym10, and to determine the genes that confer resistance to BaYMV or BaMMV in Korea. Using the traditional visual scoring of symptoms at 4 locations over 3 years, average disease rate values differed (P < 0.001) among the genotypes. ELISA test revealed the presence of both BaYMV and BaMMV in all of the field sites but Jinju and significantly different rates of infection among genotypes and years. Barley genotypes differed in how virus quantities and pathogen-induced symptoms were correlated, especially in response to BaYMV. Disease incidence was affected by the climatic conditions present during the early growing stage before overwintering. A Chinese landrace, 'Mokusekko 3', carrying rym1 and rym5 was comparatively resistant at all locations studied. The barley genotypes carrying either rym6 or rym9 were susceptible to the viral strains. The genotypes carrying rym5 were resistant in Jinju and Milyang but susceptible in Iksan and Naju. The resistance genes rym2 and rym3 were effective in local strains and would be potent contributors to disease resistance.

A Study on Generation of Free Stereo Mosaic Image Using Video Sequences (비디오 프레임 영상을 이용한 자유 입체 모자이크 영상 제작에 관한 연구)

  • Noh, Myoung-Jong;Cho, Woo-Sug;Park, June-Ku
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.27 no.4
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    • pp.453-460
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    • 2009
  • For constructing 3D information using aerial photograph or video sequences, left and right stereo images having different viewing angle should be prepared in overlapping area. In video sequences, left and right stereo images would be generated by mosaicing left and right slice images extracted in consecutive video sequences. Therefore, this paper is focused on generating left and right stereo mosaic images that are able to construct 3D information and video sequences could be made for the best use. In the stereo mosaic generation, motion parameters between video sequences should be firstly determined. In this paper, to determine motion parameters, free mosaic method using geometric relationship, such as relative orientation parameters, between consecutive frame images without GPS/INS geo-data have applied. After determining the motion parameters, the mosaic image have generated by 4 step processes: image registration, image slicing, determining on stitching line, and 3D image mosaicking. As the result of experiment, generated stereo mosaic image and analyzed result of x, y-parallax have showed.

Characterization of an Isolate of Cucumber mosaic virus Isolated from Chinese aster (Callistephus chinensis) (과꽃에서 분리한 Cucumber mosaic virus의 성질)

  • Oh, Sun-Mi;Kim, Sung-Ryul;Hong, Jin-Sung;Ryu, Ki-Hyun;Lee, Gung-Pyo;Choi, Jang-Kyung
    • Research in Plant Disease
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    • v.14 no.3
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    • pp.229-232
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    • 2008
  • An isolate of Cucumber mosaic virus (CMV), designated as Cas-CMV, was isolated from Chinese aster (Callistephus chinensis) showing severe mosaic symptom, and its properties was compared to the well-characterized Fny-CMV (subgroup IA) and As-CMV (subgroup IB) by host reaction in several indicator plants, dsRNA analysis, RT-PCR analysis, and restriction enzyme profile of the PCR products. Cas-CMV differed markedly in their host reaction to Fny-CMV or As-CMV in Cucurbita pepo cv. Black beauty. In the zucchini squash, all strains induced chlorotic spot on inoculated leaves and mosaic symptoms on upper leaves. However, symptoms induced by Cas-CMV were developed lethal necrosis on the young plants 15 to 20 days after inoculation. In experiments of dsRNA analysis and RT-PCR analysis, properties of Cas-CMV was come within subgroup I CMV. Moreover, restriction enzyme analysis using HindIII of the RT-PCR products showed that Cas-CMV belong to a member of CMV subgroup IA.