• The Plant Pathology Journal
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• 제25권3호
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• pp.236-240
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• 2009

Tylenchulus semipenetrans, citrus nematode is an important phytopathogenic nematode and responsible for serious damage on citrus. However, little information is available about genetic variability of T. semipenetrans among different populations with variation of conventional diagnostic characteristics. In this study, we compared the morphometric and genetic characteristics among different populations. The mature female of T. semipenetrans collected in this study had thicker cuticle than those in the previous studies. In comparative sequence analysis of T. semipenetrans populations obtained from Jeju in Korea, we observed genetic variations within clones generated from single individuals. To determine whether variability among copies of nuclear ribosomal DNA sequences exists in the genome of T. semipenetrans, PCR-RFLP technique from individuals of Korean isolates with MseI and MspI restriction enzymes was used to prove experimentally that all populations have intra-specific variations. Restriction enzyme digestion created several fragments on 3.0% agarose gel corresponding to several haplotypes in all populations, though some populations displayed fragment deletion. The total length of fragments was larger than before digestion, indicating sequence heterogeneity within the genome of T. semipenetrans.

• Animal cells and systems
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• 제8권2호
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• pp.111-115
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• 2004

The epidermis of the mudskipper, Periophthalmus modestus, consists of three layers- the outermost layer, middle layer and stratum germinativum. Extensive fine blood capillaries are present near the superficial layer of epidermis and outermost layer in five fins and a sucking disc. The diffusion distance between the vascular capillaries and the surface of epidermis ranged from 3.6 to 10.9${\mu}$m: 3.6 ${\mu}$m in the sucking disc, 10.9 ${\mu}$m in the anal fin and 4.6 to 5.0 ${\mu}$m in the two dorsal fins. Rate of the surface area of respiratory epithelium, the surface area of the fine blood capillaries occupied per surface area of epidermis in 0.1mm, is 3.7 to 4.4% in two dorsal fins and 1.1% in the anal fin. The middle layer is simpler in structure consisting of small or voluminous cells swollen by epidermal cells, and this layer appeared web-like. Well-developed lymphatic spaces containing lymphocytes existed in the stratum germinativum. The five fins and sucking disc had no epidermal glands.

• 한국음향학회:학술대회논문집
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• 한국음향학회 2000년도 하계학술발표대회 논문집 제19권 1호
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• pp.233-236
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• 2000

The acoustical response of fish depends on size and physical structure na, most important, on the presence or absence of a swimbladder. Acoustic scattering models for swimbladdered fish represent a fish by an ideal pressure-release surface having the size and shape as the swimbladder. Target strength experiments of red seabream (Chrysophrys major) have been conducted using 38 (split-beam), 120 (split-beam) and 200kHz (dual-beam) frequencies. At each start of each experiment, the live fish are placed in the cage at the surface, then the cage is lowed to about $4{\cal}m$ depth where it remains during the measurements. To test the acoustic models, predictions of target strength based on swimbladder morphometries of 10 red seabream offish total length from $103{\cal}mm{\;}to{\;}349{\cal}mm$ ($3 <$TL/\lambda$ < 45)are compared with conventional target strength measurements on the same, shock-frozen immediately after caged experiments. X-ray was projected along dorsal aspect to know the morphological construction of swimbladder. and fish body. At high frequencies, Helmholtz-kirchhoff(HK) approximation would greatly enhance swimbladdered fish modeling. Sound scattering model [HK-ray approximation model] for comparison to experimental target strength data was used to model backscatter measurements from individual fish. The scattering data can be used in the inverse method along with multiple frequency sonar systems to investigate the adequacy of classification and identification of fish

• 한국음향학회지
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• 제20권5호
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• pp.100-109
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• 2001

부레를 가진 어류의 산란 강도를 조사하기 위하여 길이가 다른 참돔 (red seabream, pagrus major)을 이용한 음향 실험을 실시하였다. 대상 어류를 살아 있는 상태로 - (1) 망 내에 각각 넣은 후, (2) 자유 유영 상태로 놓은후 - 3개의 음향센서 (38, 120, 200kHz)를 이용하여 길이에 따른 산란강도를 측정하였다. 실험 결과 길이에 따른 참돔의 산란 강도는 각 주파수별로 TS/sub 38㎑/＝20 log/sub 10(l)/ -66.41, TS/sub 120㎑/＝20 log/sub lo(1)/ -71.80, TS/sub 200㎑/＝20 log/sub 10(1)/ -73.94로 나타났다. 실험 결과와 이론 모델을 비교하기 위하여 어류의 형태를 근거로 한 Helmholtz-Kirchhoff 음선 근사법을 사용한 결과, 산란강도는 어류 몸체보다는 부레에 의한 효과가 95%이상으로 나타났다. 한편, 수신된 산란강도 자료를 이용한 확률밀도 함수로부터 어류의 행태에 따른 산란강도 변위는 망 내의 산란체에서는 가우스 확률밀도함수 (Gaussian PDF)로, 자유 유영 상태에서는 레일리 확률밀도함수 (Rayleigh PDF) 특성을 나타냈다.