• Composites Research
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• 제27권5호
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• pp.183-189
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• 2014

광학 현미경 관찰을 통해 산화 그래핀의 형상, 크기 및 두께를 쉽게 파악할 수 있는 광학 관찰을 위한 최적 조건을 확보하고자 하였다. 본 연구에서는 $SiO_2$ 절연막이 300 nm 두께로 도포된 실리콘 기판 위의 산화 그래핀을 하이드라진 증기 환원을 통하여 본래의 모폴로지를 유지한 채 환원된 산화 그래핀의 이미지의 선명도를 증가시켰고, 녹색 필터를 사용한 관찰을 통해 이미지의 대비값을 보다 증대시켰다. 추가적으로 얻어진 광학 이미지를 RGB 채널별로 분리하는 방법을 제안하고 이를 통해 이미지를 분석하였다. 그 결과 하이드라진 증기 환원 처리 및 녹색 파장에서의 광원 하에서 고대비의 이미지 확보가 가능하였으며, 더불어 광학 이미지의 RGB 채널 분리만으로도 선명한 그래핀 이미지를 얻을 수 있음을 알아내었다.

• 수산해양교육연구
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• 제27권4호
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• pp.1109-1117
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• 2015

최근 우리나라에서는 필리핀 현지에서 채집되는 Anguilla marmorata와 A. bicolor pacifica의 실뱀장어 수입량이 증가하는 추세이다. 본 연구에서는 2014년 5월과 7월에 필리핀의 루손섬 북쪽과 민다나오섬 남쪽의 기수지역에서 채집된 실뱀장어를 대상으로 현지에서 적용 가능한 등지느러미의 위치와 꼬리지느러미의 색소세포 침착형태 등의 외부 형태적 특징을 바탕으로 한 판별기법을 유전자분석과 함께 실시하여 그 유효성을 검토하였다. 5월 루손섬 북쪽의 시료는 A. marmorata가 90% 이상이었으며, 민다나오섬 남쪽의 시료는 A. mamorata와 A. bicolor pacifica가 각각 36.7%, 63.3%였다. 7월의 민다나오섬 남쪽의 시료는 A. mamorata와 A. biolor pacifica가 각각 46.7%, 53.3%로, 두 종간의 외부 형태학적 특징을 바탕으로 하는 종 분류기법은 유전자분석 결과와 일치하여 유효한 것으로 사료된다.

• 생태와환경
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• 제41권4호
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• pp.431-440
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• 2008

Turbid water or suspended sediment is associated with negative effects on aquatic organisms; fish, aquatic invertebrate, and periphyton. Effects of turbid water on fish differ depending on their developmental stage and a level of turbidity. Low turbid water may cause feeding and predation rates, reaction distance, and avoidance in fish, and it could make fish to die under high turbidity and long period. Therefore, it is very important to find out how turbid water or suspended sediment can affect fish in domestic watersheds. The objectives of this study were 1) to introduce international case studies and their standards to deal with suspended sediment, 2) to determine acute toxicity in 4 major freshwater fishes, and 3) to determine in relation to adverse effect of macroinvertebrates and fish. Impacts of turbid water on fish can be categorized into direct and indirect effects, and some factors such as duration and frequency of exposure, toxicity, temperature, life stage of fish, size of particle, time of occurrence, availability of and access to refugia, etc, play important role to decide magnitude of effect. A review of turbidity standard in USA, Canada, and Europe indicated that each standard varied with natural condition, and Alaska allowed liberal increase of turbidity over natural conditions in streams. Even though acute toxicity with four different species did not show any fatal effect, it should be considered to conduct a chronic test (long-term) for more detailed assessment. Compared to the control, dominance index of macroinvertebrates was greater in the turbid site, whereas biotic index, species diversity index, species richness index, and ecological score were smaller in the turbid site. According to histopathological analysis with gills of macroinvertebrate and fishes, morphological and physiological modification of gills due to suspended sediments can cause disturbance of respiration, excretion and secretion. In conclusion, in order to maintain good and healthy aquatic ecosystem, it is the best to minimize or prevent impact by occurrence of turbid water in stream and reservoir. We must make every effort to maintain and manage healthy aquatic ecosystem with additional investigation using various assessment tools and periodic biomonitoring of fish.

• 한국콘텐츠학회논문지
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• 제20권5호
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• pp.609-622
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• 2020

사회가 발달하면서 법령의 규모는 점차 증가하고 있을 뿐만 아니라 내용도 복잡해지고 있다. 법령 내에 존재하고 있는 안전기준의 규모 또한 증대되고 있고 전문화되고 있어 안전기준 상호간의 상충이나 중복 등을 최소화하기 위한 통합관리에 어려움이 있다. 안전기준의 통합관리를 위해서는 기본적으로 법령 내의 안전기준을 검색하여 추출하는 기술이 우선 확보되어야 한다. 본 연구에서는 한정된 시간 및 인력 등의 한계를 고려하여 몇 가지 특정사례를 기반으로 안전기준 자동검색 지원도구를 개발하고자 하였다. 이를 위해 우선 기존 연구에서 분류된 안전기준과 행정안전부에서 고시한 안전기준을 대상으로 안전기준이 가진 특성을 분석하고 2018년에 국립재난안전연구원에서 추출한 안전기준을 포함하는 법령정보를 수집하였다. 수집된 법령을 정제하고 형태소 분석을 실시하여 안전기준 시소러스를 구축한 후 색인 작업을 거쳐 안전기준 검색도구를 개발하였다. 향후 안전기준을 찾는데 자동검색도구가 일상적으로 적용된다면 방대하고 복잡한 안전기준의 중복 또는 상충 문제해결에 도움이 될 것으로 기대된다.

• Journal of Microbiology
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• 제45권6호
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• pp.553-557
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• 2007

In order to detect and identify the most toxic Bacillus thuringiensis strains against pests, we isolated a B. thuringiensis strain (Bn1) from Balaninus nucum (Coleoptera: Curculionidae), the most damaging hazelnut pest. Bn1 was characterized via morphological, biochemical, and molecular techniques. The isolate was serotyped, and the results showed that Bn1 was the B. thuringiensis serovar, kurstaki (H3abc). The scanning electron microscopy indicated that Bn1 has crystals with cubic and bipyramidal shapes. The Polymerase Chain Reactions (PCRs) revealed the presence of the cry1 and cry2 genes. The presence of Cry1 and Cry2 proteins in the Bn1 isolate was confirmed via SDS-PAGE, at approximately 130 kDa and 65 kDa, respectively. The bioassays conducted to determine the insecticidal activity of the Bn1 isolate were conducted with four distinct insects, using spore-crystal mixtures. We noted that Bn1 has higher toxicity as compared with the standard B. thuringiensis subsp. kurstaki (HD-1). The highest observed mortality was 90% against Malacosoma neustria and Lymantria dispar larvae. Our results show that the B. thuringiensis isolate (Bn1) may prove valuable as a significant microbial control agent against lepidopteran pests.

• The Plant Pathology Journal
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• 제31권3호
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• pp.278-289
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• 2015

Indigenous strains of Trichoderma species isolated from rhizosphere soils of Tea gardens of Assam, north eastern state of India were assessed for in vitro antagonism against two important tea fungal pathogens namely Pestalotia theae and Fusarium solani. A potent antagonist against both tea pathogenic fungi, designated as SDRLIN1, was selected and identified as Trichoderma viride. The strain also showed substantial antifungal activity against five standard phytopathogenic fungi. Culture filtrate collected from stationary growth phase of the antagonist demonstrated a significantly higher degree of inhibitory activity against all the test fungi, demonstrating the presence of an optimal blend of extracellular antifungal metabolites. Moreover, quantitative enzyme assay of exponential and stationary culture filtrates revealed that the activity of cellulase, ${\beta}$-1,3-glucanase, pectinase, and amylase was highest in the exponential phase, whereas the activity of proteases and chitinase was noted highest in the stationary phase. Morphological changes such as hyphal swelling and distortion were also observed in the fungal pathogen grown on potato dextrose agar containing stationary phase culture filtrate. Moreover, the antifungal activity of the filtrate was significantly reduced but not entirely after heat or proteinase K treatment, demonstrating substantial role of certain unknown thermostable antifungal compound(s) in the inhibitory activity.

• 대한공간정보학회지
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• 제21권4호
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• pp.37-44
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• 2013

최근 기상 이변에 따라 단시간에 집중되는 돌발홍수에 의해 해마다 막대한 인적, 물적 피해를 입고 있다. 이러한 국지성호우에 의한 산지하천이나 미소하천에서 첨두유출량을 예측하기 위한 도구로서 GIS를 적용하고 있는 추세이다. 하지만 수문학적 접근이 주를 이루고 있으며 GIS를 이용한 지형분석으로의 접근은 매우 미비한 실정이다. 본 연구에서는 돌발홍수를 발생시키는 강우량을 GIS기법과 GcIUH의 모형을 연계하여 산정하였고, 유역별 GcIUH 매개변수를 추출하여 한계유량에 따른 GcIUH 매개변수간의 상관관계를 분석하였다.

• Ocean Science Journal
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• 제41권4호
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• pp.201-205
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• 2006

Larval specimens of Halicampus punctatus were collected off Ulsan and Uljin in December 2002 (three specimens) and off Ulsan in December 2003 (one specimen). These specimens are characterized by the following morphological characteristics: rings, 14 + 35 = 49; subdorsal rings, 1 + 3 = 4; dorsal fin rays, 19 - 20; pectoral fin rays, 14 - 15; anal fm rays, 9; head length (HL), 5.8 - 6.7 in the standard length; snout length, 1.9 in HL; snout depth, 3.7 - 5.2 in snout length. The number of caudal fin rays 9 is less than those of the other species in the same genus 10. Wide stripe bands composed of small pigments are shown in the trunk and the tail. Melanophores are not found in the dorsal fin, the pectoral fin, and the anal fin except the caudal fin. The supraoccipital crest is on the head. The frontal ridge is on the dorsal side of front trunk. The blanched ridges on the opercular are fused with a main ridge like a tree branch. A few branched ridges that are small and narrow on the trunk and the tail are fused with the main ridges. We report these specimens as the first record in Korea and name them 'Byeol-sil-go-ki' in Korean.

• 한국전기전자재료학회논문지
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• 제24권7호
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• pp.599-604
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• 2011

A highly strained nanostructure comprising crystallographically aligned HgTe nanoinclusions and a surrounding PbTe matrix has been synthesized using a precipitation process of supersaturated HgTe-PbTe alloys. From the early precipitation stage, HgTe nanoinclusions take disk shape, which is transformed from initial HgTe nuclei, although there is no lattice constant difference of the two end components at standard state. As a primary reason for the morphological transformation of the initial spherical HgTe nuclei to HgTe nanodisks, the induced lattice mismatch is suggested. On the condition that the HgTe nanodisks maintain perfect coherent nature with PbTe matrix, the stress-free lattice constant of constrained HgTe nanodisks has been calculated based on the defined concept of the strain-induced tetragonality, the linear elasticity and the actual measurement in HRTEM images.

• 한국주거학회논문집
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• 제24권6호
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• pp.123-132
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• 2013

This study covers centrality of villas after Renaissance and architectural forms corresponding to the nature in the aspect of Karl Popper and Gilles Deleuze. In addition, the researcher tries to understand the changes in architectural forms according to the changes in places. To do that, Pascal and Hans Meyer's theory was introduced as a major theory. The researcher tries to set up the standard by analyzing the architectural form related with the nature and the form of center in the housing through Villa Rotonda and Villa Emo. And with key analysis of the centrality and architectural form corresponding to the nature and the four houses that are historically and morphologically important. It is found that due to the development in modern technology and materials, a variety of architectural approach is developed and they are implemented as various architectural forms. However, due to various theories of modern architectural approach, it is found that the morphological differences of suburban housing exists by forming a universal relationship constructed by four architects. The researcher expects to be understood and realized in real life construction.