• Anatomy and Cell Biology
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• 제56권1호
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• pp.61-68
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• 2023

Anatomical knowledge of the occipital condyle (OC) and its relationships to surrounding structures is important for avoiding injury during craniovertebral junction (CVJ) surgeries. This study was conducted to evaluate the morphology and morphometry of OC and its relationship to foramen magnum, jugular foramen (JF), and hypoglossal canal (HC). Morphometric parameters including length, width, height, and distances from the OC to surrounding structures were measured. The oval-like condyle was the most common OC shape, representing for 33.0% of all samples. The mean length, width and height of OC were 21.3±2.4, 10.5±1.4, and 7.4±1.1 mm, respectively. Moreover, OC was classified into three types based on its length. The most common OC length in both sexes was moderate length or type II (62.5%). The mean distance between anterior tips and posterior tips of OC to basion, and opisthion were 11.5±1.4, 39.1±3.3, 25.2±2.2, and 27.4±2.7 mm, respectively. The location of intracranial orifice of HC was commonly found related to middle 1/3 of OC in 45.0%. JF was related to the anterior 2/3 of OC in 81.0%, the anterior 1/3 of OC in 12.5%, and the entire OC length in 6.5%. These morphological analysis and morphometric data should be taken into consideration before performing surgical operation to avoid CVJ instability and neurovascular structure injury.

• 한국정밀공학회지
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• 제29권5호
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• pp.578-583
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• 2012

The pharmacological therapies and whole body vibration as non-pharmacological therapies were known to have adverse side effects. Therefore, partial stimulation was suggested and its effects were evaluated. This study aimed to evaluate the site-specific effects of partial stimulator for treatment of osteoporosis induced by estrogen deficiency. Sixteen virginal Sprague-Dawley rats (12 weeks old) were divided into 2 groups(no stimulation, stimulation groups). All rats were ovariectomised to induce osteoporosis. After 3 weeks of operation, the right tibiae in rats of stimulation group (frequency: 10Hz, cycle: 1500, strain on bone surface: $2000{\mu}{\varepsilon}$) were stimulated perpendicularly at right tibia by using partial stimulator for 6 weeks (3days/week). The right tibiae in rats were scanned, before stimulation (0 week) and at 6 weeks after stimulation by using in-vivo micro computed tomography. For investigation of changes in morphological characteristics, structural parameters were measured and calculated. At 6 weeks the morphological characteristics (relative value) in stimulation group were significantly enhanced than those in no stimulation group (p<0.05). In this study, we find that after 6 weeks of partial stimulation, the morphological characteristics of tibia trabecular bone were enhanced. Thus, we concluded that partial stimulation could be used to treat osteoporosis.

• Molecules and Cells
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• 제25권2호
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• pp.163-171
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• 2008

The genus Cynodon comprises ten species. The objective of this study was to evaluate the genetic diversity of Korean bermudagrasses at the morphological, cytological and molecular levels. Morphological parameters, the nuclear DNA content and ploidy levels were observed in 43 bermudagrass ecotypes. AFLP markers were evaluated to define the genetic diversity, and chromosome counts were made to confirm the inferred cytotypes. Nuclear DNA contents were in the ranges 1.42-1.56, 1.94-2.19, 2.54, and 2.77-2.85 pg/2C for the triploid, tetraploid, pentaploid, and hexaploid accessions, respectively. The inferred cytotypes were triploid (2n = 3x = 27), tetraploid (2n = 4x = 36), pentaploid (2n = 5x = 45), and hexaploid (2n = 6x = 54), but the majority of the collections were tetraploid (81%). Mitotic chromosome counts verified the corresponding ploidy levels. The fast growing fine-textured ecotypes had lower ploidy levels, while the pentaploids and hexaploids were coarse types. The genetic similarity ranged from 0.42 to 0.94 with an average of 0.64. UPGMA cluster analysis and principle coordinate analysis separated the ecotypes into 6 distinct groups. The genetic similarity suggests natural hybridization between the different cytotypes, which could be useful resources for future breeding and genetic studies.

• Ocean and Polar Research
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• 제32권4호
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• pp.369-377
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• 2010

To characterize ecotoxicological responses to a natural estrogen, $17{\beta}$-estradiol, we evaluated the life-history of the parental ($F_0$) and first generation ($F_1$) of the harpacticoid copepod, Tigriopus japonicus sensu lato. We evaluated the survival of nauplii and copepodites, the number of days until the emergence of copepodites and adult males, the sex ratio, brooding success, and the first brooding day of adult females. No significant differences in the survival rate were noted in response to treatments with different concentrations of $17{\beta}$-estradiol. However, $17{\beta}$-estradiol induced developmental delay and skewed the sex ratio toward males. Copepod development was delayed significantly in the 0.1 and $1\;{\mu}g\;l^{-1}$ $17{\beta}$-estradiol treatment groups relative to the control group, with a more pronounced delay in the $F_1$ group. Body length and biomass were significantly smaller in the $17{\beta}$-estradiol treated groups than in the controls. The male emergence of T. japonicus s.l. was very high in the 10 and $30\;{\mu}g\;l^{-1}$ $17{\beta}$-estradiol treatment group. Furthermore, exposure to $17{\beta}$-estradiol resulted in morphological deformities such as shrinking and swelling of the urosome, twisted setae of the caudal rami, setal loss of swimming legs, abnormal segmentation of antennules, and dwarfism.

• Journal of Biosystems Engineering
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• 제42권3호
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• pp.227-233
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• 2017

Purpose: Morphological properties of soybean roots are important indicators of the vigor of the seed, which determines the survival rate of the seedlings grown. The current vigor test for soybean seeds is manual measurement with the human eye. This study describes an application of a machine vision technique for rapid measurement of soybean seed vigor to replace the time-consuming and labor-intensive conventional method. Methods: A CCD camera was used to obtain color images of seeds during germination. Image processing techniques were used to obtain root segmentation. The various morphological parameters, such as primary root length, total root length, total surface area, average diameter, and branching points of roots were calculated from a root skeleton image using a customized pixel-based image processing algorithm. Results: The measurement accuracy of the machine vision system ranged from 92.6% to 98.8%, with accuracies of 96.2% for primary root length and 96.4% for total root length, compared to manual measurement. The correlation coefficient for each measurement was 0.999 with a standard error of prediction of 1.16 mm for primary root length and 0.97 mm for total root length. Conclusions: The developed machine vision system showed good performance for the morphological measurement of soybean roots. This image analysis algorithm, combined with a simple color camera, can be used as an alternative to the conventional seed vigor test method.

• 한국발생생물학회지:발생과생식
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• 제23권1호
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• pp.11-19
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• 2019

The study was conducted to investigate the effects of alpha-linolenic acid (ALA) combined with bovine serum albumin (BSA) or methyl-beta-cyclodextrin (MBCD) on plasma and acrosomal membrane damages, mitochondrial activity, morphological abnormality, motility, and oxidative stress in frozen-thawed boar sperm. In previous our study, 3 ng/mL ALA had been shown protective effect during freezing process of boar sperm. Therefore, we used 3 ng/mL ALA in present study and ALA was combined with same molar ratio of BSA or MBCD (ALA+BSA and ALA+MBCD, respectively). To confirm the effect of two carrier proteins, same volume of BSA and MBCD without ALA were added during cryopreservation. Membrane damage, mitochondrial activity, reactive oxygen species (ROS) and lipid peroxidation (LPO) levels were measured using flow cytometry, and movement of sperm tail as motility parameter and morphological abnormality were observed under light microscope. In results, all of sperm parameters were enhanced by ALA combined with BSA or MBCD compared to control groups (p<0.05). Mitochondrial activity, morphological abnormality, ROS and LPO levels in ALA+BSA or MBCD groups were no significant difference compared with ALA, BSA and MBCD treatment groups. On the other hand, plasma and acrosomal membrane intact, and sperm motility in ALA+MBCD group were higher than single treatment groups (p<0.05), whereas ALA+BSA did not differ. Our findings indicate that carrier proteins such as BSA and MBCD could improve the effect of ALA during cryopreservation of boar sperm, and treatment of ALA with carrier proteins enhance membrane integrity, mitochondrial activity through reduction of ROS-induced LPO.

• 한국수산과학회지
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• 제30권2호
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• pp.297-304
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• 1997

한국산과 중국산 피조개를 대상으로 형태적 차이와 RAPD (Random amplified polymorphic DNA) 기법을 이용한 유전적 차이점을 비교 연구하였다. 실험에 사용한 피조개는 경남 진해만에서 천연채묘된 치패와 중국 산동성 영성시에서 인공채묘된 치패를 채집하여 사용하였다. 두 집단의 형태적 차이는 패각형질의 부위별 길이와 무게를 계측하여 계측형질별 상관관계로 조사하였다. 그리고 유전적 차이점은 primer 종류별로 생성된 RAPD band 들간의 유사정도로 비교하였다. 각장에 대한 최대방사능길이의 상대성장율이 두 집단에서 뚜렷한 차이 $(P\leq0.05)$를 나타내어, 중국산이 한국산보다 패각의 후단부가 길다는 것을 알 수 있었다. 방사능의 수는 한국산 피조개가 $36\~41$ 였고, 중국산이 $39\~43$조 였다. RAPD-PCR 결과 19가지의 primer들로 부터 각각 $2\~6$개의 band를 볼 수 있었다. 한국산과 중국산 피조개간의 유전적 유사도는 0.29로 매우 낮아 두 집단 간의 유전적 구별이 명확하게 나타났다. 또한 한국산 피조개 개체별 유전적 유사도는 $0.58\~0.40$로, 중국산은 $0.48\~0.32$로 한 국산 피조개가 중국산보다 개체별 유전적 유사도가 높았다.

• Membrane and Water Treatment
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• 제9권1호
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• pp.43-51
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• 2018

Hollow fiber (HF) membranes are gaining wide interest over flat membranes due to their compaction and high area to surface volume ratio. This work addresses the fabrication of HF from polysulfone (PS) and polyethersulfone (PES) using N-methylpyrrolidone (NMP) as solvent in addition to other additives to achieve desired characteristics. The semi-pilot spinning system includes jacketed vessel, four spinneret block, coagulation and washing baths in addition to dryer and winder. Different parameters affecting dry-wet spinning phase inversion process were investigated. Dope compositions of PES, NMP and polyvinyl pyrrolidone (PVP) of varying molecular weights as additive were addressed. Some critical parameters of importance were also investigated. Those include dope flow rate, air gap, coagulation & washing baths and drying temperatures. The measured dope viscosity was in the range from 1.7 to 36.5 Pa.s. Air gap distance was adjusted from 20 to 45 cm and coagulation bath temperature from 20 to $46^{\circ}C$. The HF membranes were characterized by scanning electron microscope (SEM), atomic force microscope (AFM) and mechanical properties. Results indicated prevalence of finger like structure and average surface roughness from about 29 to 78.3 nm. Profile of stress strain characteristics revealed suitability of the fibers for downstream interventions for fabrication of thin film composite membrane. Different empirical correlations were formulated which enable deeper understanding of the interaction of the above mentioned variables. Data of pure water permeability (PWP) confirmed that the fabricated samples fall within the microfiltration (MF)-ultrafiltration (UF) range of membrane separation.

• Computers and Concrete
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• 제22권5호
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• pp.481-492
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• 2018

In this paper, a new hybrid fiber system (NHFS) is investigated for the application of slab. The steel fiber, polyvinyl alcohol (PVA) fiber and calcium carbonate ($CaCO_3$) whisker is added to form NHFS. The four-point bending test is carried out on the flexural properties of slab with plain, steel fiber, traditional hybrid fiber system (THFS) and NHFS reinforced cementitious composites. The flexural behavior is evaluated by ASTM C1018-97, JCI-SF4 and post-crack strength (PCS) technique. The evaluation parameters of flexural toughness such as toughness index (TI), equivalent flexural strength (EFS) and PCS are determined. The size of slab specimens is $15mm(thickness){\times}50mm(width){\times}200mm(length)$. The results show that adding $CaCO_3$ whisker to THFS can significantly improve the flexural strength, TI, EFS, PCS of the slab. The empirical relation between reinforcing index ($RI_v$) and flexural parameters show that flexural parameters of slabs increase first and then decrease; which indicates that optimum $RI_v$ values can be helpful in the considering the mix design of steel-PVA fibers-$CaCO_3$ whisker composites for achieving the desired flexural-related properties. The scanning electron microscopy is performed to observe the micro-morphological characteristics of the fracture surface, which proved the positive hybrid effect among the different fibers in cementitious composites. The NHFS can arrest the generation and propagation of the crack from micro to macro level.

• 한국항공우주학회지
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• 제35권1호
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• pp.10-17
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• 2007

Part 1에서 수행된 검정금파리의 “8자 운동”에 대한 해석결과는 와류들이 양력과 추력발생에 중요한 역할을 하는 것으로 나타났다. 곤충날개에서 발생되는 공기역학적 힘은 레이놀즈수와 같은 공기역학적 요소와 더불어 진동수, 운동진폭, 운동성분 등과 같은 운동학적적 요소들 또 날개의 형태 및 개수등과 같은 형태학적 요소들에 따를 것으로 생각된다. 본 연구에서는 레이놀즈수, 진동수 그리고 운동성분에 따른 양력과 추력발생을 고찰함으로써 이들이 공기역학적으로 어떤 영향을 미치는가를 조사하였다. 이로써 part 1의 결과를 물리적으로 좀 더 상세하게 설명할 수 있는 근거를 찾고자 하였다. 해석결과 “8자 운동”을 구성하는 운동성분 중 회전운동이 추력발생에 지배적인 역할을 하고 있었으며, 또한 추력발생은 반시계 방향의 회전순환과 깊은 관련이 있었다.