• Title/Summary/Keyword: morphological characteristics analysis

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Morphological Characteristics and Principal Component Analysis of Plums (자두의 형태적 특성과 주성분 분석에 의한 품종군 분류)

  • Chung, Kyeong-Ho
    • Horticultural Science & Technology
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    • v.17 no.1
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    • pp.23-28
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    • 1999
  • To examine taxonomic relationships among 53 plums derived from Prunus cerasifera, P. domestica, and P. salicina, principal component analysis (PCA) and cluster analysis on 27 morphological characters were conducted. Of 27 characters, leaf size, leaf shape, and leaf hair were useful characters for plum identification and understanding of taxonomic relationships among them. Leaf length, petiole length, number of leaf nectaries, leaf shape, leaf base, and date of full blooming showed the clear differences between P. salicina group and P. domestica group. Results of cluster analysis using scores of the first three principal components indicated that 53 plums could be grouped into P. salicina-P. cerasifera, P. domestica, and P. spinosa phenon at 1.0 of average distance in UPGMA. Although PCA was useful for rough classification of plums, much more characters were needed for the exact classification.

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Evaluation of Morphological Characteristics and RAPD Analysis in Korean Landraces of Naked Barley (재래종 쌀보리의 형태적 특성 및 RAPD에 의한 유연 관계 분석)

  • Cho, Won-Kyong;Lee, Jeong-Min;Kwon, Moo-Sik;Chung, Tae-Young
    • Journal of Plant Biotechnology
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    • v.29 no.4
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    • pp.217-222
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    • 2002
  • Barley varieties collected from 1940 to 1951 allover the Korean peninsula by Dr. Takahashi Ryuhei were reintroduced from the Research Institute for Bioresouces in Okayama University, Japan, and the evaluation of morphological characteristics and RAPD analysis were performed. Totally, 493 varieties of Korean barley landraces were planted in the green house, from which seeds of 491 varieties were harvested and conserved in the seedbank of the Rural Development Administration. Majority of the naked barley varieties showed dense spikes with long awn, late heading, winter habits, and long plant height. However, variants having various phenotypes such as short awn, blue aleurone color, brachytic type and waxyness were also identified. Plant height, spike length, and cold-tolerance in the varieties were also highly variable among them. Homogeneity tests on the variation of growth habits, spike density, anthocyanin pigmentation on the seed coat, and hairiness on leaf sheath between naked and covered barley showed that the variations of naked barley were similar to those of covered barley. It maybe indicate that the most of naked barley landraces were mutated from the covered barley landraces. Korean landraces of naked barley were broadly divided into 4 groups by the dendrogram produced by morphological characteristics; however, the identities of the group were rather indistinct. Many varieties, belonged to the same group, were showed different band patterns in RAPD analysis using 5 different primer sets. These results indicate that the 112 varieties of naked barley landraces were different genotypes.

Interspecific Similarity of the Subgenus Haploxylon in Korea Based on Pollen Morphological Characters (한국에 생육하는 잣나무아속의 화분형태학적 특성에 의한 종간 유사성)

  • 최태기
    • Korean Journal of Plant Resources
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    • v.17 no.2
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    • pp.202-212
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    • 2004
  • The present study was conducted to compare of pollen morphological characteristics for five Haploxylon species in Korea using light microscopy(LM). The results are as follows; 1. Highly significant (P<0.01) interspecific difference was observed in five Haploxylon species for their pollen morphological parameters. 2. The discreminant analysis based on the pollen morphological parameters demonstrated that the classification ratio of Haploxylon was 68.8 % ranging from 72.8 % of Pinus pumila to 62.2 % P. koraiensis. 3. The relationship among the species based on their pollen morphological parameters showed that P. koraiensis and P. pumila in Haploxylon were most closely related while P. pumila and P. bungeana were least related.

Morphological differences between Water deer and Sika deer ovaries during estrus and pregnancy

  • Ji-Hye Lee;Yong-Su Park;Min-Gee Oh;Sang-Hwan Kim
    • Journal of Animal Reproduction and Biotechnology
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    • v.38 no.2
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    • pp.62-69
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    • 2023
  • Background: Research on the reproductive physiology of Water and Sika deer, an endemic in Korea, still needs to be completed. This study analyzed the ovarian development and morphological characteristics of wild Water deer and Sika deer. Methods: Water deer and Sika deer ovaries were collected from the Korean Peninsula and Russia-Korean Peninsula border during the estrus and pregnancy seasons, respectively. And, morphological and physiological analysis and immunohistochemistry were conducted to confirm the detection of Ca2+ and assess the morphological changes in the ovaries. Results: The results of morphological analysis of ovaries during pregnancy and estrus, the development of the corpus luteum and follicles of Water deer showed similar patterns to other mammals. In contrast, the corpus luteum of Sika deer differed in tissue morphology and composition from Water deer. Ca2+ related to tissue metabolism was detected in the theca cells zone of Water deer on the estrus and was highly detected in the luteum cells zone during pregnancy. The hormone receptor protein expression patterns were generally higher in the ovaries of Water deer on the estrus and the pregnancy than in Sika deer. The expression of LH receptor was relatively low in the lutein cell zone, unlikely that of Water deer. The expression of VEGF was also different from Water deer, and the response in Sika deer was relatively very low compared to Water deer in expressing all proteins-related development. Conclusions: Therefore, the results of the study were shown that the composition of the corpus luteum of Sika deer is not clear compared to Water deer, and there are many differences in the functional and morphological formation of the corpus luteum.

Phylogenetic Placement and Morphological Characterization of Sclerotium rolfsii (Teleomorph: Athelia rolfsii) Associated with Blight Disease of Ipomoea batatas in Korea

  • Paul, Narayan Chandra;Hwang, Eom-Ji;Nam, Sang-Sik;Lee, Hyeong-Un;Lee, Joon-Seol;Yu, Gyeong-Dan;Kang, Yong-Gu;Lee, Kyeong-Bo;Go, San;Yang, Jung-Wook
    • Mycobiology
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    • v.45 no.3
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    • pp.129-138
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    • 2017
  • In this study, we aimed to characterize fungal samples from necrotic lesions on collar regions observed in different sweetpotato growing regions during 2015 and 2016 in Korea. Sclerotia appeared on the root zone soil surface, and white dense mycelia were observed. At the later stages of infection, mother roots quickly rotted, and large areas of the plants were destroyed. The disease occurrence was monitored at 45 and 84 farms, and 11.8% and 6.8% of the land areas were found to be infected in 2015 and 2016, respectively. Fungi were isolated from disease samples, and 36 strains were preserved. Based on the cultural and morphological characteristics of colonies, the isolates resembled the reference strain of Sclerotium rolfsii. Representative strains were identified as S. rolfsii (teleomorph: Athelia rolfsii) based on phylogenetic analysis of the internal transcribed spacer and large subunit genes along with morphological observations. To test the pathogenicity, sweetpotato storage roots were inoculated with different S. rolfsii strains. 'Yulmi' variety displayed the highest disease incidence, whereas 'Pungwonmi' resulted in the least. These findings suggested that morphological characteristics and molecular phylogenetic analysis were useful for identification of S. rolfsii.

Morphological and molecular investigation of Melosira octogona Schmidt (Melosirales, Bacillariophyta) isolated from the Jeju Island

  • Sung Min An;Kichul Cho;Grace Choi
    • Ocean and Polar Research
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    • v.46 no.2
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    • pp.123-129
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    • 2024
  • Melosira octogona Schmidt has been documented primarily through illustrations and light microscopy photographs, resulting in incomplete morphological descriptions. In this study, the morphological characteristics of M. octogona were investigated using scanning electron microscopy, and the small subunit rDNA of the strain isolated from the coast of Jeju Island, South Korea, was analyzed for the first time. The cells were organized in short chains, with the frustules taking on an octagonal shape in the girdle view because of the straight, obliquely oriented valve margin. The cells were joined together in specific pairs by a cingulum. Imaging analysis via SEM revealed the presence of a hemispherical valve with a well-developed corona on the valve face, along with stellate thickening and rimoportulae. The epicingulum was composed of six ligulate bands punctuated by rows of circular to elongated pores. Genetic analysis of the 18S rDNA sequence suggested a close relationship between M. octogona and Melosira sp. (KJ961704), demonstrating a high degree of similarity. Meanwhile, it exhibited comparatively low similarity (99.0 %) to Melosira cf. octogona (AY485518). The results of our analysis can enhance the precise recognition of M. octogona and enrich our understanding of its phylogenetic position within the order Melosirales.

Intraspecific Morphological Characteristics and Genetic Diversity of Korean Calanthe

  • Cho, Dong-Hoon;Chung, Mi-Young;Jee, Sun-Ok;Kim, Chang-Kil;Chung, Jae-Dong;Kim, Kyung-Min
    • Korean Journal of Plant Resources
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    • v.23 no.6
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    • pp.541-549
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    • 2010
  • The present study researched morphological characteristics and analyzed the genetic diversity by using RAPD in Calanthe species, native plant in Jeju, Korea. Twenty-six samples were selected by flower color, and 19 horticultural traits were investigated to study morphological characteristics. The C. discolor had the smallest leaf, the length and width of dorsal sepal, lateral sepal, petal, central lip, lateral lip, and flower stalk length were shortest and/or smallest except the spur and ovary length in Calanthe species, but those of Calanthe discolor for. sieboldii (Dence.) Ohwi (Calanthe discolor for. sieboldii) were the largest and/or biggest, and those of variants were the intermediate between C. discolor and C. discolor for. sieboldii, but spur length was the longest in C. discolor, the shortest in C. discolor for. sieboldii, and intermediate in the variants. Ovary length in C. discolor was shortest and C. discolor for. sieboldii and variants were similar with each other. The flower colors of C. discolor were brownish red, the value of CIE Lab was between 40 and 50. The flower color of C. discolor for. sieboldii was yellowish; the value of CIE Lab was between 110 and 130. And variants had various colors between 50 and 70 in the value of CIE Lab. After analyzing multiple band patterns of PCR products, 154 bands were selected as polymorphic RAPD markers. The analysis of Genetic distance of Calanthe species using RAPD showed that C. discolor and C. discolor for. sieboldii are more distant from each other than variants, and demonstrated the fact that genetic position of variants is between the other two species.

Determination of ginsenosides in Asian and American ginsengs by liquid chromatography-quadrupole/time-of-flight MS: assessing variations based on morphological characteristics

  • Chen, Yujie;Zhao, Zhongzhen;Chen, Hubiao;Brand, Eric;Yi, Tao;Qin, Minjian;Liang, Zhitao
    • Journal of Ginseng Research
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    • v.41 no.1
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    • pp.10-22
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    • 2017
  • Background: Asian ginseng and American ginseng are functional foods that share a close genetic relationship and are well-known worldwide. This article aims to investigate the correlation between morphological characteristics and the inherent quality of Asian and American ginsengs. Methods: In this study, an ultra-HPLC-quadrupole/time-of-flight MS (UHPLC-Q/TOF-MS) method was established for the quantitative analysis of 45 ginseng samples. The method developed for determination was precise and accurate. Results: The results showed that Asian ginseng samples with the same growing time (with the same or similar number of stem scars) that had a thinner main root, a longer rhizome and more branch roots contained greater amounts of ginsenosides. For American ginseng, two tendencies were observed in the relationship between the diameter of the main root and contents of ginsenosides. One tendency was that samples with thinner main roots tended to contain higher levels of ginsenosides, which was observed in the samples sold under the commercial name pao-shen. Another tendency was that samples with thicker main roots contained higher contents of ginsenosides, which was observed in the samples sold under the commercial name pao-mian, as well as in samples of American ginseng cultivated in Jilin, China. Conclusion: An approach using ultra-HPLC-quadrupole/time-of-flight MS was successfully established to link morphology and active components for evaluating the quality of Asian and American ginsengs. Clear correlation between visible morphological features and quality of Asian and American ginsengs was found. People can see the difference; this means consumers and vendors can evaluate ginseng by themselves.

Morphological Characteristics and Karyotypic Analysis of Aster spathulifolius According to Native Area

  • Yoon Pyung-Sub;Park Hye-Mi;Kim Dong-Min;Kim Hyun-Hee
    • Plant Resources
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    • v.8 no.3
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    • pp.244-249
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    • 2005
  • The growth charateristics and karyotypes of Aster spathulifolius collected from 5 sites including coastal and island region on the Korean peninsula, were analysed. Several morphological characteristics of the plants such as leaf length, leaf width, top internode, medium internode, spike branching, flower diameter, number of petal, leaf color, leaf form, stem and leaf hair, viscosity, and serration of the plants were distinctly different depending on the native region from which they were collected. Karyotypic analysis showed that the chromosome number was all diploid (2n=18), with one pair of submetacentric satellite chromosomes. The chromosome composition included 7 pairs of metacentric chromosomes and 2 pairs of submetacentric chromosomes in all plants. However, chromosome order and the ranges of the chromosome lengths were a little different from plant to plant according to their native growing regions. The plants from Geoje-Do especially showed large differences in the chromosome lengths between the longest and the shortest compared to the plants from other places. This results provide important data to support the classification of the species into several sub-species.

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Morphological Analysis of Fibers of the Seip Textiles through the Microscopic Examination (현미경 관찰을 통한 Seip 직물의 섬유 형태 연구)

  • 안춘순
    • Journal of the Korean Society of Costume
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    • v.21
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    • pp.87-99
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    • 1993
  • The fiber morphologies of the textiles from the burials of the Seip group of Ohio Hopewell (ca. 100 B.C. to A.D. 500)mounds were examined using light and scanning electron microscopy. A theoretical model was proposed which delineates the rate of morphological change in fibers through time and the cultural activities which occur during the life of a textile element. The Seip textiles were categorized according to their visual and structural characteristics. The microscopic examination of the Seip textiles yielded the evidence of animal hair fibers and bast fibers. The animal hair fibers of the seip textiles were identified as the hair of rabbit or hare. The bast fibers of the Seip textiles exhibited variation in morphological features such as the degree of bundle separation and the presence of transverse striations, fibrillations, or trans-verse cracks.

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