• Korean journal of applied entomology
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• v.57 no.3
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• pp.191-197
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• 2018

Mites in the family Ascidae are soil dwelling predators of small insects which would have potential to be used as biological control agent. In Korea, 10 genera of 24 species were recorded in the family. During the soil acarine biodiversity study, Asca bicornis Canestrini & Fanzago, 1877 was discovered from moss habitat on Muljangori craer lake 936 m alt in Hallasan mountain in Jeju which was designated as UNESCO Biosphere reserve and World herigate site. We reports this as a new record to Korean Mesostigmata fauna, and provide the morphological description. This species has morphologically characteristic as all dorsal setae nude and needle shaped, lateral caudal projection of Z4 shaped, which are different from the con-general species of A. aphidioides.

• Parasites, Hosts and Diseases
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• v.54 no.4
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• pp.485-496
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• 2016

We describe 2 echinostome species recovered from an Eastern cattle egret, Bubulcus ibis coromandus, from Cheongju-si (city), Chungcheongbuk-do (province), Korea. Total 72 Pegosomum bubulcum specimens were recovered from the bile duct. They were $7,566{\times}2,938{\mu}m$ in average size and had 27 collar spines with vitelline extension from anterior 1/3 level of the esophagus to mid-level of the posterior testis as characteristic features. Total 9 specimens of Nephrostomum ramosum were recovered in the small intestines of the bird. They were ribbon-shaped, $11,378{\times}2,124{\mu}m$ in average size, and morphologically variable in some organs, i.e., the number of collar spines (47-50), the shape of ovary and testes, and the extension of vitelline follicles. These morphological variations observed in a single host indicated that these features are not critical for the classification of Nephrostomum species and thus were reconsidered taxonomically as synonym of N. ramosum. This study is the first report documenting and describing both flukes and their associated genera in Korea.

• The Plant Pathology Journal
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• v.19 no.3
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• pp.181-183
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• 2003

Corky root symptoms caused by Pyrenochaeta lycopersici were observed on the roots and stem base of tomato plants in Korea. Symptoms on infected plants typically appeared as stunting and generally lacking vigor, and infected plants die back from the foliage tips after fruits have set. Brown lesions appearing with bands around the roots were characteristic symptoms of the disease. The lesions become swollen and cracked along the length of the root with corky appearance. Based on cultural and morphological characteristics, the fungus from the diseased plants was identified as Pyrenochaeta lycopersici. Pycnidia were solitary, globose to subglobose, brown to black, darker around the neck region, and measured 173-215 $\mu\textrm{m}$ in diameter with septate setae up to 102-132$\times$6.5 $\mu\textrm{m}$. Conidia were hyaline, unicellular, and 4.2-4.7$\times$l.5-2.0 $\mu\textrm{m}$ long. Optimum temperature for mycelial growth of the p. lycopersici isolates ranged from $20^{\circ}C$ to $25^{\circ}C$. Fifteen isolates off lycopersici were tested for pathogenicity to susceptible and tolerant cultivars of tomato plants by artificial inoculation. Three isolates of P. lycopersici induced typical corky root discoloration on susceptible tomato cultivars but not on tolerant tomato. This is the Erst report in Korea of tomato corky root disease caused by P. lycopersici.

• Parasites, Hosts and Diseases
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• v.53 no.6
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• pp.719-724
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• 2015

Nematomorpha, horsehair or Gordian worms, include about 300 freshwater species in 22 genera (Gordiida) and 5 marine species in 1 marine genus (Nectonema). They are parasitic in arthropods during their juvenile stage. In the present study, the used gordian worm was found in the feces of a dog (5-month old, male) in July 2014. Following the worm analysis using light and scanning electron microscopes, the morphological classification was re-evaluated with molecular analysis. The worm was determined to be a male worm having a bi-lobed tail and had male gonads in cross sections. It was identified as Gordius sp. (Nematomorpha: Gordiidae) based on the characteristic morphologies of cross sections and areole on the cuticle. DNA analysis on 18S rRNA partial sequence arrangements was also carried out, and the gordiid worm was assumed to be close to the genus Gordius based on a phylogenic tree analysis.

• Korean Journal of Veterinary Research
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• v.29 no.4
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• pp.531-540
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• 1989

A new sudden death in rabbits appeared in China and Korea in 1984 and 1985, respectively, and was recognized to be an acute infectious disease caused by a virus. The disease was reported as a "new viral disease," and thereafter, a tentative name of "viral hemorrhagic disease", "hemorrhagic pneumonia" or "viral hemorrhagic pneumonia" has been described in the case reports. But authors had called the viral disease "rabbit viral hepatitis" due to picornavirus infection, because the principal lesion of the disease was an acute hepatitis. The purpose of this report is to describe the electron microscopic findings on the livers in experimentally infected rabbits. All the livers of the affected rabbits were shown to have degenerative changes of a type that is characteristic of acute hepatitis. In the liver cells, there were dilation of rER and mitochondria, vacuole formation of various sizes, and appearances of many virus-like particles in the vicinity of rER, granular bodies and crystalline arrays of viral particles in the cytoplasm with necrotic changes of the nucleus. Clusters of virus-like particles and viral crystals appeared in the cytoplasm of sinusoid endothelial cells and Kupffer's cells with morphological changes of organelles. Also viral crystals were demonstrated in the cytoplasm of macrophages among the liver cells. On the whole, the liver cells had many virus-like particles and a few crystalline arrays of viral particles. Therefore, this implies that the liver cells are the main site of the viral replication in inducing the viremia. It was concluded that the liver was the primary target organ of this viral disease, and the pathological and the ultrastructural evidence suggest that the virus may be belong to genus enterovirus.

• The Korean Journal of Food And Nutrition
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• v.27 no.2
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• pp.213-218
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• 2014

The enterotoxin production and antimicrobial susceptibility on hemolytic strains from stools of diarrheal pigs was investigated in this study. Through morphological observation, gyrB nucleotide sequence, and API kit analysis, the selected potential pathogenic strain BY06 was identified as Bacillus cereus. Because the characteristic of enterotoxin symptoms were widely caused by Bacillus cereus strains, a PCR test was carried out in order to check the enterotoxin genes (hblA) in this strain. According to the results, this strain was an enterotoxin positive strain containing the hblA gene. The minimum inhibitory concentrations of 10 different antimicrobial agents were screened by the agar dilution test, indicating that this strain was resistant to penicillin G and intermediate to erythromycin; however, it susceptible to cephalothin, vancomycin, clindamycin, fusidic acid, gentamicin, ciprofloxacin, tetracycline and rifampin. These results suggest that the B. cereus BY06 isolated from pig feces has a potential risk of producing enterotoxin and is resistant to penicillin G, but susceptible to various antimicrobial agents.

• Asian Journal of Atmospheric Environment
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• v.6 no.4
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• pp.275-280
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• 2012

To understand the effect of aerosols on the growth and physiological conditions of trees in forests, it is important to know the state of aerosols that are deposited on the surface of the leaves or needles. In this study, we developed methods of visualization of submicron-sized aerosols that were artificially deposited from the gas-phase or liquid phase onto tree leaves or needles in trees. Firstly, we used field-emission scanning electron microscopy (FE-SEM) to observe black carbon (BC) particles that were artificially sprayed onto the leaves or needles. The distribution of BC particles deposited on the leaves and needles were distinguished based on the size and morphological features of the particles. The distribution and agglomerates size of BC particles differed between two spraying methods of BC particles employed. Secondly, we tried to visualize gold (Au) particles that were artificially sprayed onto the leaves using energy dispersive X-ray spectrometry (EDX) coupled to FE-SEM. We detected the Au particles based on the characteristic X-ray spectrum, which was secondarily generated from the Au particles. In contrast to the case of BC particles, the Au particles did not form agglomerates and were uniformly distributed on the leaf surfaces. The present results show that our methods provide useful information of adsorption and/or behavior of fine particles at the submicron level on the surface of the leaves.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2008.10a
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• pp.431-435
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• 2008

2006년 11월 이후 신 차량 번호판 등장 후, 신 차량 번호판과 구 차량 번호판이 혼합되어 있다. 이에 따라 속도위반, 신호위반 단속, 무인 주차관리 시스템, 범죄 및 도주 차량 검거, 고속도로 톨게이트에서 통행료 지불로 인한 교통 체증현상을 해소하기 위한 자동 요금 징수와 같은 다양한 경우에서 자동차 번호판의 특징에 맞는 인식 시스템이 요구되고 있다. 따라서 본 논문에서는 이러한 문제를 해결하기 위해 형태학적 특징 및 차 연산과 ART2 알고리즘을 이용한 차량 번호판 인식 방법을 제안한다. 무인 카메라에서 획득된 차량 번호판 영상에서 차 연산을 이용하여 에지를 추출한 후에 블록 이진화를 한다. 이진화 된 차량 영상에서 신 구 차량 번호판의 형태학적 특성을 8방향 윤곽선 추적 알고리즘에 적용하여 잡음 영역을 제거하고, 차량의 번호판 영역을 추출한다 추출된 번호판 영역에 대하여 평균 이진화와 최대 최소 이진화를 적용하여 번호판의 개별 영역에 대한 형태학적 특성을 고려하여 잡음을 제거하고, Labeling 알고리즘을 적용하여 개별 문자를 추출한 후에 결합한다. 이렇게 분류된 개별 문자 및 숫자 코드를 ART2 알고리즘에 적용하여 학습 및 인식을 한다. 제안된 차량 번호판 추출 및 인식 방법의 성능을 평가하기 위해 녹색 번호판과 흰색 번호판 이미지 각각 100장을 대상으로 실험한 결과, 제시 된 차량 번호판 추출 및 인식 방법이 실험을 통해서 효율적인 것을 확인하였다.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2007.10a
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• pp.413-417
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• 2007

2006년 11월 이후 신 차량 번호판 등장 후, 신 차량 번호판 차량이 꾸준히 증가하고 있다. 이에 따라 속도위반, 신호위반 단속, 무인 주차 관리 시스템, 범죄 및 도주 차량 검거, 고속도로 톨게이트에서 통행료 지불로 인한 교통 체증현상을 해소하기 위한 자동 요금 징수와 같은 다양한 경우에서 신 자동차 번호판의 특징에 맞는 인식 시스템이 요구되고 있다. 따라서 본 논문에서는 이러한 문제를 해결하기 위해 지능형 신 자동차 번호판 인식 방법을 제안한다. 무인 카메라에서 획득된 신 차량 영상을 그레이 레벨로 변환한 후에 블록 이진화한다. 블록 이진화된 차량 영상을 대상으로 차량의 형태학적 특징을 적용하여 잡음을 제거한 후, 번호판 영역을 추출한다. 추출된 번호판 영역에 대해 Grassfire 알고리즘을 적용하여 개별 코드를 추출한다. 차량 번호판을 인식하기 위하여 추출된 개별 코드를 퍼지 ART 알고리즘을 적용하여 학습 및 인식한다. 제안된 차량 번호판 추출 및 인식 방법의 성능을 평가하기 위해 100장의 차량 영상을 대상으로 실험한 결과, 제안된 차량 번호판 추출 및 인식 방법이 실험을 통해서 효율적인 것을 확인하였다.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.1
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• pp.107-113
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• 2015

This study was conducted to find out the effective induction method of tetraploid plants to obtain potential data for cultivating superior varieties by colchicine treatment. The seed germination were decreased by the higher concentration of colchicine treatment and longer soaking time. A total of 907 individuals were germinated in 16 treated plots except control (untreated plot) and 28 tetraploids were induced which was about 3.1% of the number of seed germinated. The plant regeneration rate by colchicine treatment on explant of Prunella vulgaris for. albiflora Nakai under in vitro culture was decreased with the higher concentration of colchicine. While a total of 312 individuals were regenerated in all treatments, the explant was soaked in more than 0.05% for over 1 hour, tetraploid could be obtained. In particular, for the soaking treatment in 0.05% for 6 hours and 12 hours, 37 tetraploids were induced, which was about 57.8% of the number of plant regenerated. In accordance with the observation on doubling of DNA contents in leaf in order to identify polyploid, the peak DNA content of G1 phase was 101.3 for diploid and 197.2 for tetraploid. The result confirmed the doubling of DNA content. Furthermore, the number of chloroplasts per guard cell depending on polyploid was around 10 in diploid and 19.3 in tetraploid, which was around 1.9 times as much as diploid.