• Journal of Microbiology
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• 제43권2호
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• pp.158-165
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• 2005

Psilocybe fagicola comprises a complex of more than eight species, six of them in Mexico, and all of them possessing a long pseudorhiza, a characteristic not listed by Heim and Cailleux in 1959 in the original description of the type species, but described by Guzman in 1978 and 1983. The description of Psilocybe fagicola s.s. is here emended to include the length of the cheilocystidia of(6-) 12-20 (-30) llm, as well as the absence or scarcity of pleurocystidia. Psilocybe xalapensis and P. wassoniorum are considered to be synonymous with P. fagicola s.s. However, Psilocybe banderillensis and P. herrerae from Mexico, P. columbiana from Colombia, and P. keralensis from India are considered to be valid species within this complex. Moreover, P. novoxalapensis and P. teofilae, both from Mexico, are described as new species. Length of spores, presence or absence of pleurocystidia and their variations, and type of cheilocystidia constitute the principal defining characteristics of the species. Setaceous hyphae at the base of the stipe, as well as caulocystidia, lack taxonomic value, as do other morphological characteristics, including pileipellis and subpileipellis. A key to the eight considered species is also presented within the paper.

• Fisheries and Aquatic Sciences
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• 제18권2호
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• pp.137-142
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• 2015

Antifreeze proteins and glycoproteins [AF(G)Ps] constitute a group of proteins that lower the freezing but not the melting points of aqueous solutions, enabling polar and north-temperate fish to survive in ice-laden environments. However, little is known about antifreeze activity in temperate fish; such work would extend our knowledge on the functions and evolution of AF(G)Ps. In the present study, we screened for antifreeze activity in temperate fish caught off the coast of Jumunjin ($37.89^{\circ}N$), Gangneung, Korea. Thermal hysteresis (TH) and the ability to inhibit ice recrystallization (IR) in blood, liver, and muscle samples from nine fish were examined to assess antifreeze activity. As the East Sea off the coast of Jumunjin is ice-free year round, we thought it most unlikely that the fish would express antifreeze proteins. Surprisingly, the blood of Pleurogrammus azonus and three types of tissue from Gymnocanthus herzensteini, Zoarces gilli and Kareius bicoloratus exhibited measurable TH values together with the ability to trigger characteristic morphological changes in ice crystals. Blood samples from the three species also evidenced ice recrystallization (IR) inhibition. This implies that AF(G)Ps or other antifreeze-like substances are present in temperate fish even under nonfreezing conditions. These results contribute to our understanding of the functions and origins of antifreeze activity in fish.

• 패션비즈니스
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• 제12권6호
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• pp.23-33
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• 2008

The physico-chemical characteristics by bleaching treatments were assessed by several instrumental analyses such as surface morphology, chemical structural change, color change as well as tensile strength. The change of morphological characteristic was observed through scanning electron microscope(SEM). The observation of the fine structure on hair surface by SEM showed the bleached hair had much damaged to hair cuticle, and some of cuticle surface were worn away. To investigate the chemical structural changes in hair keratin, the cross-sections of hair samples were directly analysed using Fourier transform infrared microspectroscopy(FT-IRM). The results showed the cysteic acid S=O band intensity was distinctively increased by performing the bleaching treatment. The cleavage of cystine was appeared to proceed primarily through the sulfur-sulfur (-S-S-) fission whereby cysteic acid was formed as a principal oxidation products. The distribution of amide I band in hair keratin was determined by attenuated total reflectance(ATR) FT-IR mapping image. The results showed that the outer side of hair cortex was more damaged than the inner side of the hair cortex. Also, during chemical bleaching of the hair with alkaline peroxide, the hair was turned to reddish yellow due to the oxidative degradation of eumelanin. This means the eumelanin is more unstable than pheomelanin in chemical oxidation. With bleaching, the tensile strength was also reduced as a results of the chemical oxidation.

• 한국농공학회논문집
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• 제57권5호
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• pp.89-99
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• 2015

In this research, 1,032 data of precise safety inspection from 2004 to 2013 are gathered and constructed for finding effective safety inspection systems. Items are extracted from constructed data and factors for typology are decided with statistical method such as principle component analysis and cluster analysis. For factor decision, we extruded independent characteristics such as morphological and geographical characteristic, and deleted items which can be expressed by combination of independent characteristics. Four factors such as total storage, watershed ratio, levee length ratio, and spillway length ratio are extracted in this process. In cluster analysis, levee length ratio is excluded because it is not separated as cluster. Finally nine types of agricultural reservoir are extruded by total storage, watershed ratio, and spillway length ratio with frequency analysis.

• Archives of Pharmacal Research
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• 제21권5호
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• pp.521-526
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• 1998

The physicochmical properties of recombinant hepatitis B surface antigen (r-HBsAg), which was expressed in C127 mammalian cell were studied. Using roller bottle culture in DMEM supplemented with fetal bovine serum, 10-15 mg/L of r-HBsAg was produced with about 31% of purification yield. The purity of r-HBsAg by HPLC was 99.8% and electron microscopic examination showed homogeneous spherical particle with 22 nm in diameter, a morphological characteristic of HBsAg. The density of r-HBsAg by CsCI density gradient method was 1.19g/ml and the isoelectric point by Mono $P^{TM}$ HR 5/20 column was 4.6. The analysis of subunit protein pattern using SDS-PAGE followed by scanning densitometry gave 81.3% of S protein and 18.7% of pre-S protein. fluorophore-assisted-carbohydrate-electrophoresis analysis showed the relative amount of carbohydrate to protein was 1.7% and it smajr component was N-acetyl glucosamine, which was about 39% of total carbohydrate. The relative amount of lipid to protein determined by vanillin phosphoric acid method was 32.5% and its major component was phospholipid, which was about 70% of total lipid. The physicochemical properties of C127 mammalian cell-derved r-HBsAg are similar to those of p-HBsAg, suggesting that the r-HBsAg can be used in developing a new preventive vaccine against hepatitis B.

• Natural Product Sciences
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• 제22권3호
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• pp.209-215
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• 2016

Kigelia africana (Lam.) Benth. (Bignoniaceae) is a flowering plants in South, Central and West Africa and commonly known as the sausage tree (Eng.); worsboom (Afr.); umVunguta, umFongothi (Zulu); Modukguhlu (North Sotho); Muvevha (Venda). The dried, powdered fruits are used as dressing for wounds and ulcers, haemorrhoids, rheumatism, purgative, skin-firming, lactation in breast-feeding mothers. The aim of this study is to investigate the cytotoxic and apoptotic potentials of 70% ethanolic extracts of Kigelia africana fruits in HCT116 human colon cancer cells. Treatment of Kigelia africana fruits with various concentrations resulted in a sequence of characteristic of apoptosis, including loss of cell viability and morphological changes. Flow cytometry analysis showed Kigelia africana fruits increased the sub-G1 phase (apoptosis) population. Apoptosis confirmed by annexin V-fluorescein isothiocyanate and propidium iodide double staining in HCT116 human colon cancer cell lines. Moreover, analysis of the mechanism indicated that Kigelia africana fruits showed an increased Bax and Bcl-2 expressions in a dose-dependent manner, resulting in activation of hallmarks of apoptotic events, caspase-3, caspase-9 and cleaved poly-ADP-ribose polymerase. This is the first report to demonstrate the cytotoxicity of Kigelia africana fruits on HCT116 human colon cancer cells.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2007년도 춘계종합학술대회
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• pp.252-257
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• 2007

우리나라의 항만은 수 출입화물의 99.5%를 처리하며, 육로 및 철도 수송 물동량의 기종점 역할을 수행하는 중요한 곳으로서 항만 물동량의 신속한 처리와 자동화 시스템에 의한 비용절감은 엄청난 효과를 가져온다. 따라서 본 논문에서는 항만에서 취급하는 컨테이너를 자동으로 식별할 수 있는 자동화 방법을 제안한다. 실제 컨테이너 영상을 그레이 영상으로 변환한 후, 프리윗 마스크(Prewitt-Mask)를 적용하여 윤곽선을 추출하고 컨테이너를 식별할 수 있는 개별 식별자의 형태학적 특징 정보를 이용하여 식별자 후보영역을 검출한다. 검출된 식별자 후보영역은 개별 식별자 영역외에 잡음 영역이 포함되어 있으므로 4방향 윤곽선 추적 알고리즘과 Grassfire 알고리즘을 적용하여 잡음을 제거하고 개별 식별자들을 각각 객체화한다. 잡음이 제거된 식별자 후보 영역에서 객체화 한 개별 식별자는 컨테이너 식별을 위해 FCM 기반 퍼지 RBF 네트워크를 적용하여 인식한다. 본 논문에서 제안한 컨테이너 식별자 인식 방법의 성능을 평가하기 위해 실제 컨테이너 영상 300장을 대상으로 실험한 결과, 기존의 방법보다 인식 성능이 개선되었음을 확인할 수 있었다.

• 폴리머
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• 제26권4호
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• pp.492-500
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• 2002

일반 PAN 섬유를 $240^{\circ}C$에서 산화시킨 후 1 N NaOH 수용액에서 가수분해시켜 oxidized-PAN 섬유 (oxy-PAN)를 제조하였다. 산과 염기용액에서의 oxy-PAN 섬유의 구조를 $^{13}C-NMR$ 분광분석법을 이용하여 분석하였다. 가수분해에 의하여 -COOH 기를 포함하는 oxy-PAN 섬유는 산과 염기 수용액에서 수축 및 팽창 거동을 하였는데, 염기성 수용액에서는 반대전하로 작용하는 $Na^+$이온이 물 분자와 함께 섬유 내부로 침투하면서 팽창하였으며, 산성 수용액에서는 $Na^+$이온이 물 분자와 함께 섬유 기부로 축출되면서 수축하였다. 친수성의 섬유내부로 산/염기 작용기가 자유롭게 침투하여 oxy-PA기 섬유의 화학적인 구조는 쉽게 변화된다는 것을 확인하였으며, 모폴로지도 pH에 의하여 영향을 받는다는 것을 관찰하였다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제13권3호
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• pp.231-240
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• 1991

The parents of twenty-five patients with cleft lip(with or without cleft palate), CL(P) and the parents of fifteen non-cleft patients were studied. Area measurements of cranium of brain case from lateral and frontal roentgenograms. The most important finding of this investigation was that the CL/CP parents had a significantly smaller brain case than did the control subjects. A smaller brain case may well be one morphological characteristic predisposing toward the cleft anomaly. The results obtained were as follows, 1) Total area measurements for brain case of parents of CWCP patients were significantly smaller than those in the control group, on the frontal view. 2) A significantly smaller parietal and occipital region on the frontal view vas noted in the parents of CL/CP patients. 3) A significantly smaller parietal region on the lateral view was noted in the mothers of CL/CP patients. 4) A significantly smaller mastoid area on the lateral view was noted in the parents of CL/CP patients.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1996년도 추계학술대회
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• pp.118-121
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• 1996

This paper describes ECG signal labeling based on Fuzzy clustering, which is necessary at automated ECG diagnosis. The NPPA(Non parametric partitioning algorithm) compares the correlations of wave forms, which tends to recognize the same wave forms as different when the wave forms have a little morphological variation. We propose to apply Fuzzy clustering to ECG QRS Complex labeling, which prevents the errors to mistake by using If-then comparision. The process is divided into two parts. The first part is a parameters extraction process from ECG signal, which is composed of filtering, QRS detection by mapping to a phase space by time delay coordinates and generation of characteristic vectors. The second is fuzzy clustering by FCM(Fuzzy c-means), which is composed of a clustering, an assessment of cluster validity and labeling.