• 미생물학회지
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• 제47권4호
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• pp.281-288
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• 2011

스트레스 반응에 관여하는 Saccharomyces cerevisiae 전사인자인 Msn2/4의 $C_2H_2$ zinc finger 부위와 아미노산 서열 유사성을 보이는 Aspergillus nidulans MsnA의 하위 유전자 획득을 위하여 msnA 결손 돌연변이체 또는 과발현 균주에서 야생주와 비교하여 차별적으로 발현되는 유전자(Differentially Expressed Gene, DEG)들을 분리하였다. 선별된 DEG들은 염기서열 결정을 통해 해당 유전자들을 동정하였고 이들 중 DEG6는 단당류 수송자(monosaccharide transporter)로 예측된 mstB 유전자로 밝혀졌다. mstB의 발현은 MsnA 과발현에 의하여 증가되었으며 MsnA는 in vitro에서 mstB 프로모터 부위에 직접적으로 결합하였다. MstB는 12개의 막결합 부위를 가지며 A. niger의 고친화성 단당류 수송자(high-affinity monosaccharide transporter)인 MstA와 80%의 높은 아미노산 서열 동일성을 보였다. mstB 결손 돌연변이체의 표현형은 야생주와 유사하였으나 MstB가 과발현된 균주는 낮은 당 농도인 0.1% glucose 배지에서 유성생식 기관인 cleistothecia의 형성이 증가하였다. 이러한 결과는 단당류 수송자인 MstB가 유성분화 과정에서 요구되는 당의 수송에 관여하고 있음을 시사한다.

• ALGAE
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• 제21권1호
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• pp.157-160
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• 2006

Three different forms of Undaria pinnatifida, the southern form (U. pinnatifida f. typica), the northern form (U. pinnatifida f. distans), and Samcheok form (recently cultivated strain), were examined for the contents and compositions of fucoidans. Fucoidans were extracted from the dried edible portions of three forms of U. pinnatifida in low pH condition, mainly by ethanol precipitation and CaCl2 treatment. It was shown that Samcheok form contains 1.8 and 3.5 times more fucoidans than the northern and the southern forms, respectively. The monosaccharide compositions of individual fucoidans were also varied. The fucoidans from the southern and the northern forms were shown to be composed of mainly fucose and galactose with the molar percentage ratios of 83.5%:16.5% and 87.4%:12.6%, respectively, indicating that these are F-type fucoidans. The fucoidan from Samcheok form, however, consisted of fucose (62.7%), galactose (32.9%), and small amount of glucose (4.4%). The results of this study showed that both amount and monosaccharide compositions of fucoidans are variable depending on U. pinnatifida forms.

• Bulletin of the Korean Chemical Society
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• 제32권7호
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• pp.2286-2300
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• 2011

We synthesized eight G8 molecular transporters (MTs) based on 4 different monosaccharide scaffolds, and studied their biological properties with a special focus on possible mitochondrial targeting and tissue selectivity. The mitochondrial affinity of these MTs was found to be clearly related to the scaffold stereochemistry and also tenuously with the lipophilicity. It may be suggested that in the practical delivery strategy of drugs for the brain and mitochondrial diseases the BBB permeability and mitochondrial affinity should be considered as key parameters, and that an enhanced mitochondrial affinity appears possible by further research on the structure-property relationship of guanidine-rich molecular transporters.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.597-600
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• 2000

In this study the enzymatic synthesis of ester-linked conjugates of amino acid and monosaccharide in pyridine was tested by the catalysis of Optimase M-440, an alkaline serine pretense. Optimase M-440 showed the higher activity in the reaction of monosaccharides which have one or more primary -OH groups. And also Optimase M-440 showed high regioselectivity; The transesterification of primary -OH group selectively occurred.

• Parasites, Hosts and Diseases
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• 제42권2호
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• pp.77-79
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• 2004

We purified specific 31/36 kDa antigenic molecules from sparganum in different intermediate hosts (snakes and mice) and analyzed their monosaccharide compositions. Compositional analysis showed that glucose and man nose concentrations were 2-3 fold higher in the 31/36 kDa molecule purified from snakes than those from mice. This result implies that antigenic glycoproteins of sparganum from snakes might be modified in mammalian sparganosis with respect to their carbohydrate composition.

• Asian-Australasian Journal of Animal Sciences
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• 제20권3호
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• pp.351-358
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• 2007

Carbohydrate-protein interactions are known to be important in gamete interactions. Several evidence indicated that a fucose-containing sulfated polysaccharide fucoidan was potential inhibitor of fertilization in vitro and thus fucose seemed to be part of the recognition signal of gamete interaction in mammals. In recent investigation we found that ${\alpha}$-L-fucosidase activity was present in boar spermatozoa and it was related to sperm binding to and penetration into zona pellucida (ZP) in vitro. The objective of this study was to determine the effects of monosaccharide L-fucose and polysaccharide fucoidan on sperm ${\alpha}$-L-fucosidase activity and relation to sperm-oocyte interaction in pig. Results indicated that the activity of sperm ${\alpha}$-L-fucosidase was largely inhibited (62%) when sperm suspension was treated with monosaccharide L-fucose. It also significantly inhibited the number of sperm binding to ZP (32%) and penetration into zona-intact oocytes (72%), but did not inhibit penetration into zona-free oocytes when fertilization medium contained L-fucose. The chlorotetracycline (CTC) assessment showed that L-fucose did not affect induction of sperm capacitation and acrosome reaction. In contrast, the activity of sperm ${\alpha}$-L-fucosidase was not inhibited when sperm suspension was treated with polysaccharide fucoidan but sperm-ZP binding was greatly inhibited (85%) and completely blocked sperm penetration into zona-intact or zona-free oocytes. The CTC assessment showed that fucoidan increased the F pattern and decreased the AR pattern sperm. These results suggested that the different inhibitory mechanisms were present between monosaccharide L-fucose and polysaccharide fucoidan on sperm-oocyte interaction, the inhibition effect of ${\alpha}$-L-fucose on sperm binding and penetrating into ZP caused sperm ${\alpha}$-L-fucosidase inhibited by ${\alpha}$-L-fucose.

• 한국작물학회지
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• 제19권
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• pp.52-58
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• 1975

대두에 함유되어 있는 Stachyose를 소화성당으로 변화시켜 식품으로서의 가치를 제고시킬 목적으로 대두 '평양'품종을 표준발아와 Hypochlorite를 처리한후 발아과정중 종자내에 함유되어있는 Monosaccharides 및 Oligosaccharides의 함량변화를 Thin-Layer Chromatography법으로 실험하였다. 1. Monosaccharides의 함량변화: 표준발아시 전기간중 Fructose가 제일함량이 많았고 Glucose, Galactose순으로 함량이 적었고 시간이 경과함에 따라 점점 감소되었다. Hypochlorite처리시 전기간중 Galactose는 전연 나타나지 않았다. 두 처리간에는 144시간을 제외하고 표준발아시켰을때가 Monosaccharide의 함량이 많았다. 2. Oligosaccharides의 함량변화: Sucrose는 표준발아시 함량은 적으나 전기간중 나타났으며96시간까지는 증가되었다가 약간씩 감소됐다.

• 한국토양비료학회지
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• 제22권3호
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• pp.191-196
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• 1989

강원도(江原道)에 위치(位置)한 점봉산(點鳳山)과 오태산(五台山)의 삼림토양(森林土壤)에서 침엽수림(針葉樹林)과 활엽수림(闊葉樹林)으로 나누어 층위별(層位別)로 채취(採取)하여 단당류(單糖類)의 함량(含量)을 분석(分析)하여 조사(調査)한 결과(結果)는 다음과 같다. 1. 단당류(單糖類)의 함량(含量)은 유기층(有機層)에 가장 많았고 심층(深層)으로 내려갈수록 감소(減少)되었는데 이는 유기물함량(有機物含量)과 직접적인 관계가 있었다. 2. Hexose (galactose, glucose, mannose)의 함량(含量)이 절대적으로 많았으며 그 다음으로는 Pentose(arabinose, ribose, xylose)의 순서이었고 deoxyhexoe(furose, rhamnese)의 함량(含量)이 가장 적었다. 單糖類의 함량(含量)은 아래와 같다. glucose>mannose>galactose>arabinose>xylose>rahmnose>fucose>ribose. 3. 침엽수림토양(針葉樹林土壤)의 유기층(有機層)에는 극히 적은 량(量)의 ribose만이 함유(含有)되어 있었으며 활엽수림토양(闊葉樹林土壤)에서는 ribose가 검출되지 않았다. 4. 전토양(全土壤) 유기물(有機物)에 대한 단당류(單糖類)의 비율(比率)은 토양(土壤)의 깊이에 따라 감소(減少)했는데 이는 부식화(腐植化)의 효과(效果)라고 생각된다. 표면유기층(表面有機層)의 단당류(單糖類) 함량(含量)은 유기탄소(有機炭素)에 대해 27~50%, 유기물함량(有機物含量)으로 환산(換算)하면 15.7~29%에 해당하며 이 중에서 hexose는 유기탄소(有機炭素)에 대해 20~30%, 유기물(有機物)에 대해서는 12~22 %의 비율(比率)로 함유(含有)되어 있었다. 5. 각각(各各)의 단당류(單糖類)가 차지하는 비율(比率)은 토양단면(土壤斷面)을 통해 종합해 볼 때 어떤 규칙적인 양상을 보이지 않았다. 이는 토양미생물(土壤微生物)의 활동(活動)이 일정치 않았기 때문이 아닌가 사료된다.

• 수도
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• 통권22호
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• pp.63-78
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• 1980

• 수도
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• 통권23호
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• pp.46-55
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• 1980