• 생명과학회지
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• 제10권6호
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• pp.564-569
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• 2000

This study was designed to investigate the effect of silkworm (Bombyx mori L.) powder on lipofuscin, acetylcholine (ACh) and its related enzyme activities in brain of rats. Sprague-Dawley (SD) male rats ($160{\pm}10g$) were fed basic diet (control group), and experimental diets (SWP-200 and SWP-400 groups) added 200 and 400 mg/kg BW/day for 6 weeks. In case of liver membranes, lipofuscin (LF) levels resulted in a slight decreases (4.6% and 11.5%, respectively) in SWP-200 and SWP-400 groups compared with control group. But in case of barin as the most sensitive organ, LF levels were remarkably inhibited about 16.7% and 20.0% in SWP-200 and SWP-400 groups compared with control group. There were no significant differences in acetylcholine (ACh) syntheses as a very important neurotransmitter, and choline acetyltransferase (ChAT) activities as a synthesis enzyme of ACh, and acetylcholinesterase (AChE) activities as a hydrolysis enzyme, which were concerned in transmission of neuron through synapses in brain of SWP-200 and SWP-400 groups compared with control group. Monoamine oxidase-B (MAO-B) activities were significantly inhibited (about 10.2%) in brain of SWP-400 groups compared with control group. These results suggest that inhibiting effects of LF accumulation and MAO-B activity of silkworm powder (SWP) may play a pivotal role in attenuating a various age-related changes for improvement of brain function.

• Archives of Pharmacal Research
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• 제19권1호
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• pp.23-29
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• 1996

Changes in dopaminergic activities were investigated after the intracerebroventricular (icv) administration of ethylcholine aziridium (AF64A) in rats. The levels of dopamine (DA) and metabolites, the activities of tyrosine hydroxylase (TH) and monoamine oxidase (MAO), and the specific binding sites of dopamine receptros in striata, hippocampus, and frontal cortex were assessed 6 days after the AF64A treatment with 3 nmol/each ventrcle. In frontal cortex, the levels of DA and metabolities were significantly decreased without changes in metabolites/DA ratios in the AF64A-treated groups. In contrast, the ratios of metabolites/DA were significantly decreased in striatum and hippocampus in the AF64A treatment. The activity of TH in frontal cortex was significantly decreased. However, that in other areas was not changed. Also the activity of MAO-A was not changed in the studied brain regions. However, the activity of MAO-B in striatum was significantly increased with no change in other areas. The specific binding sites of dopamine D1 and D2 receptors were increased in AF64A-treated frontal cortex. However, those were not changed in striatum and hippocampus except the small decreased specific binding sites of dopamine D-1 receptors in striatum after AF64A treatment. These results indicate that the dopaminergic activity was altered in AF64A treatment. Furthermore, it suggest that the decreased dopaminergic activities in each brain regions might be differently affected by AF64A treatment.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.299.3-300
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• 2002

Both of Semen (OF-Se) or stem (OF-Sf) of Opuntia ficus-indica Semen have been used as a healthful food or folk medicine in korea for the treatment of asthma. diabetes mellitus. aging, osteoporosis, rheumatic arthritis. constipation, cancer. gastric ulcer. constipation. toxic state, edema, etc, There are many reports that OF have the anti-gastric damage, wound healing, diabetes mellitus, monoamine oxidase B inhibitor etc. (omitted)

• Natural Product Sciences
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• 제19권1호
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• pp.8-14
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• 2013

The inhibitory effect and the structure-activity relationships of luteolin and its related derivatives isolated from Taraxacum mongolicum against MAO activities were investigated. The activity-guided isolation of extract from Taraxacum mongolicum led to the isolation of three flavonoids, luteolin, diosmetin, and luteolin-7-glucoside, a polyphenol, chlorogenic acid, a tyrosine and a uridine. The inhibitory activities of luteolin and its related derivatives against MAOs activities are dependent on their molecular structures. The presence of the phenolic hydroxy group at para-position is the active site for MAO-A inhibition as well as of MAO-B. The methoxy group has no potential on MAO-A inhibition. An additional phenolic hydroxy group at the ortho-position alleviates about 4-fold MAO-A inhibitory activity of phenolic hydroxy group at para-position. A carboxylic group seems to be critical for DBH inhibition and has no effects on MAO.

• 한국식품영양과학회지
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• 제34권5호
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• pp.738-742
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• 2005

고령화 현상에 의한 노인 인구의 증가에 따라 노인성 질환연구의 중요성이 대두되고 있으며, 특히 노인성 질환 중 가장 문제되는 치매(dementia)에서 노인성 치매(Alzhemier's disease)는 복합적 인 원인에 의 해서 나타나므로 다양한 관점에서의 연구가 필요하다. 따라서 본 연구에서는 마우스에 scopolamin(30 mg/kg BW)을 투여하여 기억${\cdot}$학습장애를 유발하고, 뇌조직의 아세틸콜린 및 그 관련 효소에 미치는 도토리 분말의 영향을 분석하였다. 뇌의 가장 중요한 신경전달 물질로서 아세틸콜린 생성에 미치는 도토리 분말 투여효과를 비교하였을 때, AP-5및 AP-10 투여군에서 $4.2\%$ 및 $11.3\%$의 ACh 합성이 증가하는 경향이 나타났으며, ACh의 분해효소인 acetylcholinesterase(AChE)의 활성은 AP-5 및 AP-10의 도토리 분말 투여군에서 $13.5\%$ 및 $17.6\%$로서 AChE효소활성이 유의적으로 감소하였다. 그리고 카테롤아민계 신경전달물질의 파괴에 관계하는MAO-B효소의 활성은 AP-5 및 AP-10의 도토리 분말 투여군에서 $10.0\%$ 및 $12.7\%$로서 도토리 분말 투여에 의해 MAO-B효소활성이 현저하게 억제되었다. 따라서 도토리는 AChE 효소의 활성을 저해하여 ACh의 농도를 높일 뿐만 아니라 MAO-B효소의 활성을 효과적으로 억제하여 카테콜아민계 신경전달물질을 잘 보존함으로써 치매 예방에 도움이 될 것으로 기대된다.

• 대한한방내과학회지
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• 제21권2호
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• pp.275-281
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• 2000

Object : This study was carried out to examine the effect of Bupleuri Radix on experimental cholestasis, and make clear apart of this mechanism. Methods : Two models of common bile duct ligation group and taurocholate load group after common bile duct ligation were induced, and Bupleuri Radix extract was taken orally for 14 days. In the 1, 2, 4, 7 and 14 days after treatment, the mitochondrial and microsomal monoamine oxidase(MAO) A and B activities in liver were measured. Results : The mitochondrial MAO A and B activities increased in both Blupleuri Radix treated group after common bile duct ligation and Blupleuri Radix treated group after taurocholate load and common bile duct ligation. MAO A increased in Blupleuri Radix treated group after taurocholate load and common bile duct ligation, and MAO B increased in Blupleuri Radix treated group after common bile duct ligation. The microsomal MAO A activities increased in both Blupleuri Radix treated group after common bile duct ligation and Blupleuri Radix treated group after taurocholate load and common bile duct ligation. Conclusion : According to the result, it is consider that Blupleuri Radix not only improves cholestatis in liver, but also decreases a genetic synthesis of taurocholic acid.

• 한국축산식품학회지
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• 제40권2호
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• pp.183-196
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• 2020

The protective effect of pig skin gelatin water extracts (PSW) and the low molecular weight hydrolysates of PSW generated via enzymatic hydrolysis with Flavourzyme® 1000L (LPSW) against scopolamine-induced impairment of cognitive function in mice was determined. Seventy male ICR mice weighing 20-25 g were randomly assigned to seven groups: Control (CON); scopolamine (SCO, 1 mg/kg B.W., intraperitoneally (i.p.); tetrahydroaminoacridine 10 [THA 10, tacrine; 10 mg/kg B.W. per oral (p.o.) with SCO (i.p.)]; PSW 10 (10 mg/kg B.W. (p.o.) with SCO (i.p.); PSW 40 (40 mg/kg B.W. (p.o.) with SCO (i.p.); LPSW 100 (100 mg/kg B.W. (p.o.) with SCO (i.p.); LPSW 400 (400 mg/kg B.W. (p.o.) with SCO (i.p.). All treatment groups, except CON, received scopolamine on the day of the experiment. The oxygen radical absorbance capacity of LPSW 400 at 1 mg/mL was 154.14 μM Trolox equivalent. Administration of PSW and LPSW for 15 weeks did not significantly affect on physical performance of mice. LPSW 400 significantly increased spontaneous alternation, reaching the level observed for THA and CON. The latency time of animals receiving LPSW 400 was higher than that of mice treated with SCO alone in the passive avoidance test, whereas it was shorter in the water maze test. LPSW 400 increased acetylcholine (ACh) content and decreased ACh esterase activity (p<0.05). LPSW 100 and LPSW 400 reduced monoamine oxidase-B activity. These results indicated that LPSW at 400 mg/kg B.W. is a potentially strong antioxidant and contains novel components for the functional food industry.

• 셀메드
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• 제2권2호
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• pp.19.1-19.6
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• 2012

$Capparis$ $zeylanica$ Linn. a 'Rasayana' drug is used for its memory enhancing effects in the traditional Ayurvedic system of medicine. The aim of this study was to evaluate acetylcholinesterase (AChE) inhibitory and memory enhancing activities of $Capparis$ $zeylanica$ Linn. The$in-vitro$ and $ex-vivo$ models of AChE inhibitory activity were used along with Morris water maze test to study the effect on memory in rats. The anticholinesterase effect of methanolic and aqueous extracts of $Capparis$ $zeylanica$ was measured by spectrophotometric Ellman method at 0.1, 0.3, 1.0, 3.0, 10 and 30 mg/ml and brain monoamine oxidase (MAO-A and MAO-B) activity was assessed by Naoi's method. The results $in-vitro$ and $ex-vivo$ AChE assay revealed that methanolic and aqueous extracts of $Capparis$ $zeylanica$ inhibit AChE activity, whereas these extracts did not alter MAO activity at any concentration tested as compared to moclobemide and L-deprenyl. The results indicate that $Capparis$ $zeylanica$ improves scopolamine-induced memory deficits through inhibition of AChE activity, and not by direct MAO inhibition.

• 생약학회지
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• 제40권1호
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• pp.41-45
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• 2009

Parkinson's disease (PD) is the second most common neurodegenerative disorder after Alzhemier's disease. Neuropathologically, PD is characterized by the selective loss of dopaminergic neurons. The neuronal toxicity of cytosolic excess dopamine (DA) has been described in many studies using several cell lines. In dopaminergic neurons, cytosolic excess DA is easily oxidized via monoamine oxidase (MAO)-B, tyrosinase or by auto-oxidation to produce neurotoxic metabolites such as DA quinone. So, in the present study, we induced cell death by treatment of DA ($600{\mu}M$) in human neuroblastoma SH-SY5Y cell which was treated samples before 24 hr, and cell viability was measured by fluorescence activated cell sorter (FACs) analysis. Of those tested, the extracts of Poria cocos (赤茯笭)(whole), Gastrodia elata (rhizomes), Eucommia ulmoides (炒)(barks), Syneilesis palmata (whole), Acorus gramineus (rhizomes), Ligustrum japonicum (leaves) showed neuroprotective effects in dose dependent manner.

• Journal of Nutrition and Health
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• 제37권3호
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• pp.176-181
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• 2004

솔잎 BuOH획분을 하루 25, 50, 100 mg/kg BW로써 SD계 랫트에 45일 동안 투여하여 뇌조직획분의 콜레스테롤 및 LF의 침착, ACh 및 ACh의 합성효소, ChAT, 신경전달에 관여하는 효소 AChE의 활성, 카테콜라민계 신경 전달물질의 파괴효소 MAO-B의 활성에 미치는 영향을 평가하였다. 뇌조직의 mitochnodria 및 microsome 획분 중의 콜레스테롤이 BuOH-50 및 BuOH-100 투여그룹에서 14∼17% 및 23∼34%의 통계적인 억제효과가 인정되었고, 뇌조직에 대한 LF도 BuOH-50 및 BuOH-100 투여그룹에서 10∼14%의 유의적인 침착 억제효과가 인정되었다. 뇌조직중의 신경전달물질 ACh의 함량 및 ACh의 합성효소 ChAT의 활성은 BuOH획분의 거의 용량의존적으로 증가현상이 나타났지만, BuOH-50 및 BuOH-100 투여그룹에서 각각 11∼17% 및 11∼23%의 통계적인 증가효과가 인정되었을 뿐만 아니라 AChE의 활성도 BuOH-50 및 BuOH-100 투여그룹에서 14∼17%의 통계적인 증가효과가 인정되었다. 뇌조직중의 MAO-B의 활성은 BuOH 획분의 거의 용량의존적으로 억제하였지만, 그 유의성은 인정할 수 없었다. 따라서 솔잎 BuOH 획분의 투여는 뇌조직의 유동성을 축진하고 콜레스테롤의 침착을 억제하며 ACh와 그 관련효소의 활성을 촉진하여 기억ㆍ학습의 증진에 매우 효과적으로 작용할 것으로 기대된다.