• 한국지리정보학회지
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• 제16권1호
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• pp.36-46
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• 2013

세계 최초의 정지궤도 해양관측 위성인 천리안 위성의 해색 센서인 Geostationary Ocean Color Imager (GOCI)는 2011년 4월부터 원시 자료를 생산하고 있다. 본 연구에서는 GOCI 원시 자료로부터 보다 자연색에 가까운 컬러합성영상 제작을 위한 방법론과 자료처리용 소프트웨어인 GOCI RGB Maker를 개발하였다. GOCI 원시자료는 대기보정과 재투영 기법을 이용하여 최종적으로 컬러합성영상을 제작할 수 있도록 최적화된 알고리듬을 구현하였다. 이 알고리즘이 적용된 소프트웨어는 다양한 하드웨어 환경에서도 선택적으로 관심영역과 출력창의 크기를 입력받아 처리할 수 있도록 제작되어 교육적 효과를 높였다. GOCI RGB Maker는 공개용 소프트웨어로서, GOCI 자료에 대한 이해와 활용을 증대시킬 수 있을 것이다. 또한, 정지궤도 관측 영상은 관측 영역의 환경특성 변화를 감시하는데 훌륭한 역할을 할 수 있을 것으로 기대된다.

• Journal of Microbiology and Biotechnology
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• 제18권1호
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• pp.145-152
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• 2008

A cell-based in vitro exposure system was developed to determine whether oxidative stress plays a role in the cytotoxic effects of volatile organic compounds (VOCs) such as benzene, toluene, xylene, and chlorobenzene, using human epithelial HeLa cells. Thin films based on cysteine-terminated synthetic oligopeptides were fabricated for immobilization of the HeLa cells on a gold (Au) substrate. In addition, an immobilized cell-based sensor was applied to the electrochemical detection of the VOCs. Layer formation and immobilization of the cells were investigated with surface plasmon resonance (SPR), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The adhered living cells were exposed to VOCs; this caused a change in the SPR angle and the VOC-specific electrochemical signal. In addition, VOC toxicity was found to correlate with the degree of nitric oxide (NO) generation and EIS. The primary reason for the marked increase in impedance was the change of aqueous electrolyte composition as a result of cell responses. The p53 and NF-${\kappa}B $ downregulation were closely related to the magnitude of growth inhibition associated with increasing concentrations of each VOC. Therefore, the proposed cell immobilization method, using a self-assembly technique and VOC-specific electrochemical signals, can be applied to construct a cell microarray for onsite VOC monitoring.

• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 2002년도 추계국제학술대회
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• pp.156-156
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• 2002

The goal of this study is to develop a biomarker used in monitoring abnormal behaviors of Japanese medaka (Oryzias latipes) as a model organism caused by hazardous chemicals that are toxic and persistent in the ecosystem. A widely used insecticide, diazinon (O, O-diethyl O- (2-isopropyl-4-methyl-6-pyrimidinyl) phosphorothioate), is highly neurotoxic to fish, and it is also well known that it causes vertebral malformation and behavioral changes of fish at relatively low concentrations. The fish behaviors were observed on a real time basis using an image processing and automatic data acquisition system. The genes potentially involved in the abnormal behaviors were cloned using suppression subtractive hybridization (SSH) technique. The untreated individuals showed common behavioral characteristics. When the test fish was affected by diazinon at a concentration of 0.1 and 1 ppm, some specific patterns were observed in its behavioral activity and locomotive tracks. The typical patterns were enhanced surfacing activity, opercular movement, erratic movement, tremors and convulsions as reported previously. The number of genes up-regulated tty diazinon treatment were 97 which includes 27 of unknown genes. The number of down-regulated genes were 99 including 60 of unknown genes. These gene expression patterns will be analyzed by the artificial neural networks such as self organization map (SOM) and multilayer perceptron (MLP), revealing the role of genes responsible for the behaviors. These results may provide molecular biological and neurobehavioral bases of a biomonitoring system for diazinon using a model organism such as fish.

• 한국발생생물학회지:발생과생식
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• 제15권1호
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• pp.31-39
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• 2011

Change in intracellular $Ca^{2+}$-concentration ($[Ca^{2+}]_i$) is an essential event for egg activation and further development. $Ca^{2+}$ ion is originated from intracellular $Ca^{2+}$-store via inositol 1,4,5-triphosphate receptor and/or $Ca^{2+}$ influx via $Ca^{2+}$ channel. This study was performed to investigate whether changes in $Ca^{2+}$/calmodulin dependent protein kinase II (CaM KII) activity affect $Ca^{2+}$ influx during artificial egg activation with ethanol using $Ca^{2+}$ monitoring system and whole-cell patch clamp technique. Under $Ca^{2+}$ ion-omitted condition, $Ca^{2+}$-oscillation was stopped within 30 min post microinjection of porcine sperm factor, and ethanol-induced $Ca^{2+}$ increase was reduced. To investigate the role of CaM KII known as an integrator of $Ca^{2+}$- oscillation during mammalian egg fertilization, CaM KII activity was tested with a specific inhibitor KN-93. In the eggs treated with KN-93, ethanol failed to induce egg activation. In addition, KN-93 inhibited inward $Ca^{2+}$ current ($I_{Ca}$) in a time-dependent manner in whole-cell configuration. Immunostaining data showed that the voltage-dependent $Ca^{2+}$ channels were distributed along the plasma membrane of mouse egg and 2-cell embryo. From these results, we suggest that $Ca^{2+}$ influx during fertilization might be controlled by CaM KII activity.

• 한국대기환경학회지
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• 제27권6호
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• pp.663-671
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• 2011

Although a scooter is a convenient transportation means for a short distance traveling with a light package in the congested urban center, it might be one of the significant sources of air pollutants to which many people can easily be exposed during its passing-by. In this paper, we measured concentrations of gases and particles emitted from a scooter at roadside with no other traffic. To understand the characteristics of scooter emissions with respect to driving speed (idling, 30 km/h) at the roadside, total particle number concentration, particle size distribution, average surface area of particles deposited in the alveolar region, and concentrations of black carbon, CO, and $NO_x$ were measured. The concentrations of the particle number, surface area of deposited particles, CO, and $NO_x$ were highly fluctuated in the scooter's idling condition. The trends of particle number concentration, CO, and $NO_x$ generation were similar to one another. When the scooter started to move, all of $NO_x$, CO and particle number concentrations increased and after it passed by at the speed of 30 km/h, the concentration peaks of the particles and gases appeared at the same time. Unimodal size distribution with ~70 and ~93 nm mode diameters was observed for the idling and cruising condition, respectively. From this work, we found that emission from a passing vehicle could be characterized using a roadside monitoring technique.

• Journal of Chest Surgery
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• 제30권12호
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• pp.1205-1213
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• 1997

가톨릭대학교 의과대학 부속 성바오로 병원에서 1996년 1월부터 7월까지 개심술시 경식도심초음파를 이용하여 수술 중 관찰한 61명의 증례를 분석하여 다음과 같은 결과를 얻었다. 61명의 환자 중 질병 분류는 심장 판막 질환 25명, 관상동맥 질환 22명, 선천성 심질환 13명, 심장 판막 질환과 관상동맥 질환이 병발한 경우가 1명 이었다. 1. 경식도심초음파를 이용하여 선천성 심질환 1예(심실중격결손증 재수술)와 대동맥 판막 치환술을 시행한 환자 1예에서 일차 심정지에 의한 수술 후 잔존 단락과 판막 주위 누출이 확인되어 재심정지를 유도하여 수술을 완료하였다 2. 공기색전증의 발생을 전 예에서 방지할 수 있었다. 3. 경식도심초음파로 측정한 심박출량과 온도희석법으로 측정한 심박출량은 통계학적으로 의미있는 상관관계를 보여 경식도 초음파를 이용한 심박출량 감시가 의미 있음을 시사하였다(Linear regression analysis, p<0.001). 4. 관상동맥우회로술을 시행한 23명의 환자에서 수술 중 경식도 초음파도상 이상 벽운동을 보이는 분절의 도부타민 투여 전후와 관상동맥우회로술 후의 반응을 비교 관찰하였다. 저 용량 도부타민 검사는 통계적으 \ulcorner유의하였다(민감도 76%, 특이도 94.7%, 양성 예측율 95%, 음성 예측율 75%).

• Journal of Chest Surgery
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• 제45권2호
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• pp.95-100
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• 2012

Background: The purpose of this report is to describe the perioperative outcomes of standard carotid endarterectomy (CEA) with general anesthesia, routine shunting, and tissue patching in symptomatic carotid stenoses. Materials and Methods: Between October 2007 and July 2011, 22 patients with symptomatic carotid stenosis (male/female, 19/3; mean age, $67.2{\pm}9.4$ years) underwent a combined total of 23 CEAs using a standardized technique. The strict surgical protocol included general anesthesia and standard carotid bifurcation endarterectomy with routine shunting. The 8-French Pruitt-Inahara shunt was used in all the patients. Results: During the ischemic time, the shunts were inserted within 2.5 minutes, and 5 patients (22.7%) revealed ischemic cerebral signals (flat wave) in electroencephalographic monitoring but recovered soon after insertion of the shunt. The mean shunting time for CEA was $59.1{\pm}10.3$ minutes. There was no perioperative mortality or even minor stroke. All patients woke up in the operating room or the operative care room before being moved to the ward. One patient had difficulty swallowing due to hypoglossal nerve palsy, but had completely recovered by 1 month postsurgery. Conclusion: Routine shunting is suggested to be a safe and reliable method of brain perfusion and protection during CEA in symptomatic carotid stenoses.

• 한국물환경학회지
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• 제28권6호
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• pp.890-895
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• 2012

Duration curves describe the percentage of time that a certain water quality (total/fecal coliform (=TC/FC)) or discharge is exceeded. The curves methodology are usually based on daily records and are useful in estimating how many days per year and event will be exceeded. The technique was further applied to estimated TC/FC loading to the Geumho River, using the daily mean flow rate and TC/FC concentration data during January, 2001 and December, 2011 for the Geumhogang6 (=Seongseo water level station) where an automated monitoring station is located in Gangchang-bridge. Low flow of the Seongseo (=11.1 cms) was equivalent to 75.3% on an exceedance probability scale. Load Duration curve for TC/FC loading at the Seongseo was constructed. Standard load duration curve was constructed with the water quality criteria for class III (TC/FC concentration = 5000/1000 CFU/ 100 mL). By plotting TC/FC observed load duration curve with standard load duration curve, it could be revealed that water quality do not meet the desired water quality for 68.8/11.2% on an exceedance probability scale. IF linear correlation between flow rate and coliform concentration is assumed, it can be interpreted that water quality exceed desired criteria when daily average flow rate is over 11.9/109.9 cms.

• 한국물환경학회지
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• 제36권5호
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• pp.353-363
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• 2020

Hydrologic models can be classified into two types: those for understanding physical processes and those for predicting hydrologic quantities. This study deals with how to use the model to predict today's stream flow based on the system's knowledge of yesterday's state and the model parameters. In this regard, for the model to generate accurate predictions, the uncertainty of the parameters and appropriate estimates of the state variables are required. In this study, a relatively simple hydrologic partitioning model is proposed that can explicitly implement the hydrologic partitioning process, and the posterior distribution of the parameters of the proposed model is estimated using the Markov chain Monte Carlo approach. Further, the application method of the ensemble Kalman filter is proposed for updating the normalized soil moisture, which is the state variable of the model, by linking the information on the posterior distribution of the parameters and by assimilating the observed steam flow data. The stochastically and recursively estimated stream flows using the data assimilation technique revealed better representation of the observed data than the stream flows predicted using the deterministic model. Therefore, the ensemble Kalman filter in conjunction with the Markov chain Monte Carlo approach could be a reliable and effective method for forecasting daily stream flow, and it could also be a suitable method for routinely updating and monitoring the watershed-averaged soil moisture.

• 비파괴검사학회지
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• 제19권1호
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• pp.25-33
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• 1999

콘크리트는 다상(multi-phase)의 재료로서 구성되어지는 복합재료의 일종으로써 준취성(quasi-brittle)적인 재료적 특성을 가지고 있기 때문에 실제 복잡한 미시적 파괴특성을 나타낸다. 따라서 콘크리트 구조물의 안전성 확보를 위해서는 먼저 하중의 증가에 따른 콘크리트 내부의 변형특성 및 미시적 파손기구를 파악하여야 하며, 특히 실제 구조물에 있어서는 이들 특성들을 비파괴적으로 상시 (on-line) 모니터링 하여야 할 필요가 있다. 따라서 본 연구에서는 3점 굽힘 하중을 받는 콘크리트 부재의 미시적 파손기구 및 각 파손기구에 대한 AE 특성을 명확히 함으로써 콘크리트 부재의 미시적 손상 및 파괴 특성을 비파괴적으로 평가하고 AE 발생원 위치 추정 기법을 이용하여 균열 발생 및 진전거동을 평가하였다.