• Korean Journal of Weed Science
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• v.7 no.3
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• pp.316-320
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• 1987

In order to determine effects of molinate mixture granular formulations on release rate and weed control efficacy of herbicides and growth of rice plants, zeolite impregnation, slurry, and sand-coating granules were tested in laboratory and greenhouse. Release rate of molinate and simetryn was faster in sand-coating granule than in zeolite impregnation and slurry type granules. Mixture granular formulations of molinate/simetryn or molinate/simetryn/MCPB showed good weed control efficacy and this trend was more remarkably shown in sand-coating granule. Sand-coating granule more inhibited growth of rice plants compared to zeolite impregnation and slurry type granules.

• The Korean Journal of Pesticide Science
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• v.7 no.3
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• pp.188-197
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• 2003

Endocrine disrupting chemicals (EDCs) can alter hormone regulation that control reproductive system in animals. The effects of endosulfan, molinate, and alachlor that suspected to have examined disruption EDCs effect on a fish species of interest, Xiphophorus helleri (swordtail fish), were studied using vitellogenin (Vtg) and aromatase as diagnostic biomarkers. Induction of Vtg proteins was detected by RT-PCR in male fish treated with alachlor, and mixture of endosulfan and molinate in dose response manner. Also, induction of aromatase was detected by RT-PCR in male fish treated with alachlor, endosulfan, and mixture of endosulfan and molinate in sinlilar manner. In this study, swordtail fish exposed to endosulfan or molinate individually did not show any adverse effects. However, Vtg and aromatase expressions and apoptosis were detected in swordtail. fish exposed to the mixture of endosulfan and molinate. These results suggested that low concentrations of mixture of molinate and endosulfan individually do not affect swordtail fish, but may influence genital system, and induce apoptosis.

• Korean Journal of Weed Science
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• v.18 no.4
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• pp.341-347
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• 1998

This study was carried out to investigate the effect of U-Granule formulation of molinate(S-ethylhexaphdro-1H-azepine-1-carbothioate) mixtures in green house and paddy field, Five minutes were taken for U-Granule to spread out 7m in irrigated water of direct seeded on flooded paddy surface. The concentration of active ingredient of molinate in molinate U-Granule application was similar to molinate+simetryn U-Granule application. But weeding effect of molinate+simetryn U-Granule on Echinochlor crus-galli was 23% higher Than single application of molinate at 7m from application point. Bioefficacy of molinate+simetryn+imazosulfuron U-Granule on control of Echinochloa crus-galli was higher than that of molinzte+imazosulfuton U-Granule, but those effect on Eleochairs kuroguwai was not significantly different. Only slight rice phytotoxicity was observed at 5m and 2m from application point of U-Granule molinate+simetryn+imazosulfuron and molinate+imazosulfuron, respectively. So it is concluded that there is little phytotoxicity problem in practical application of U-Granule of molinate mixtures.

• Molecular & Cellular Toxicology
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• v.2 no.3
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• pp.176-184
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• 2006

The controversy on genotoxicity of molinate, an herbicide, has been reported in bacterial system, and in vitro and in vivo mammalian systems. To clarify the genotoxicity of molinate, we performed bacterial gene mutation test, in vitro chromosome aberration and mouse lymphoma $tk^{+/-}$ gene assay, and in vivo micronucleus assay using bone marrow cells and peripheral reticulocytes of mice. In bacterial gene mutation assay, no mutagenicity of molinate ($12-185{\mu}g/plate$) was observed in Salmonella typhimurium TA 98, 100, 1535 and 1537 both in the absence and in the presence of S-9 metabolic activation system. The clastogenicity of molinate was observed in the presence ($102.1-408.2\;{\mu}g/mL$) of metabolic activation system in mammalian cell system using Chinese hamster lung fibroblast. However, no clastogenicity was observed in the absence ($13.6-54.3\;{\mu}g/mL$) of metabolic activation system. It is suggested that the genotoxicity of molinate was derived some metabolites by metabolic activation. Molinate was also subjected to mouse lymphoma L5178Y $tk^{+/-}$ cells using microtiter cloning technique. In the absence of S-9 mixture, mutation frequencies (MFs) were revealed $1.4-1.9{\times}10^{-4}$ with no statistical significance. However, MFs in the presence of metabolic activation system revealed $3.2-3.4{\times}10^{-4}$ with statistical significance (p<0.05). In vivo micronucleus (MN) assay using mouse bone marrow cells, molinate revealed genotoxic potential in the dose ranges of 100-398 mg/kg of molinate when administered orally. Molinate also subjected to acridine orange MN assay with mouse peripheral reticulocytes. The frequency of micronucleated reticulocytes (MNRETs) induced 48 hr after i.p. injection at a single dose of 91, 182 and 363 mg/kg of molinate was dose-dependently increased as $10.2{\pm}4.7,\;14.6{\pm}3.9\;and\;28.6{\pm}6.3\;(mean{\pm}SD\;of\;MNRETs/2,000\;reticulocytes)$ with statistical significance (p<0.05), respectively. Consequently, genotoxic potential of molinate was observed in in vitro mammalian mutagenicity systems only in the presence of metabolic activation system and in vivo MN assay using both bone marrow cells and peripheral reticulocytes in the dose ranges used in this experiment. These results suggest that metabolic activation plays a critical role to express the genotoxicity of molinate in in vitro and in vivo mammalian system.

• Korean Journal of Weed Science
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• v.4 no.2
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• pp.169-178
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• 1984

This experiment was conducted to find out selective herbicides which are safe to the rice seedlings and to provide effective weed control method in protected semi-irrigated rice seedbed. There was no crop injury in rice with benzophenap [2-(4-2, 4-dichloro-3-methylbenzoyl)-1, 3-dimethyl-pyrazol-5-yl-oxy)-4'-methyl acetophenone] (240g), pyrazoxyfene [1,3-dimethyl-4-(2,4-dichlorobenzoyl)-Sphenacyloxy pyrazole] (200g), chlormethoxynil [2,4-dichlorophenyl-4-nitro-3-methoxyphenyl ether] (180g), dimepiperate [S-(1-methyl-1-phenethyl)-piperidine-l-carbathioate] (210g), dimepiperate + probenazol [3-allyloxy-1,2-benzisothiazol-1,1-dioxide] (210 + 120g) mixture and dimepiperate + probenazol + molinate [S-ethyl-N,N-hexamethylene-thiol-carbamate] (120 + 120 + 120g) mixture at times of application studied. Butachlor [2-chloro-2',6'-diethyl-N-buthoxymethyl acetanilide] + pyrazolate [4-(2,4-dichlorbenzoyl)-1,3dimethyl-pyrazol-5-yl-p-toluene-sulfonate] (70 + 120g) mixture and butachlor + chlormethoxynil (60 + 120g) mixture caused root length to shorten and root viability to decrease. However, the crop injury was recovered at 25 days after seeding. Benzophenap, pyraxoxyfene and butachlor + pyrazolate mixture were effective for weed control when applied at 2 days before seeding. Chlormethoxynil and butachlor + chlormethoxynil mixture was very effective for controlling annual weed. Dimepiperate, dimepiperate + probenazol mixture and dimepiperate + probenazol + molinate mixture did not control most weeds except for Echinochloa crus-galli (L.) Beauv.

• Korean Journal of Weed Science
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• v.15 no.3
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• pp.232-237
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• 1995

Effect of azimsulfuron {1-(4,6-dimethoxypyrimidin-2-yl)-3-[1-methyl-4-(2-methyl-2H-tetrazol-5-yl) pyrazol-5-ylsulfonl]urea} combined with eight annual herbicides on shoot and root growth of rice (Oryza sativa L.) and barnyardgrass [Echinochloa crus-galli (L.) P. Beauv.] was investigated. Annual herbicides used were four thiocarbamates (dimepiperate, molinate, esprocarb, and thiobencarb), two acetanilides (butachlor and pretilachlor), one urea (dymron), and one oxadiazole (oxadiazon) herbicide. Growth inhibition in rice shoot was greater with azimsulfuron mixed with the annual herbicides than with azimsulfuron only. The azimsulfuron mixtures did not bring about decrease in growth inhibition of rice shoot. However, safening effect in root growth of rice was obtained when dimepiperate, molinate and dymron were combined with greater than 10ppm of azimsulfuron. Greater inhibition in shoot and root growth of rice occurred with straight chain hydrocarbon substitute such as esprocarb and thiobencarb than with cyclohydrocarbon substitute such as dimepiperate and molinate. Application of the azimsulfuron mixtures resulted in increase. in growth inhibition of shoot and root growth of barnyardgrass as compared with when azimsulfuron only was applied.

• Korean Journal of Weed Science
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• v.12 no.3
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• pp.271-291
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• 1992

This study was conducted to survey the situation of direct rice seeding in Honam province in Korea to investigate problems and seek countermeasure of weed control in direct rice seeding. The total area of direct rice seeding in the south-western part of Korea (Chonbuk, Chonnam, and Chungnam) was 1650.8ha (732.1ha for direct seeding in dry field and 918.7ha for direct seeding in flooding field) in 1992. The followings are summary of the study. 1. In case of direct rice seeding in dry field, butachlor EC and G at 3 to 5 DAS was mostly selected by farmers to control weeds in dry field. Benthiocarb or chlornitrofen was also used in few cases. At 10 to 14 DAS just before rice emergence, tank misture of butachlor EC and paraquat was treated by some farmers. At 35 to 40 days, after flooding mixture of sulfonylurea derivatives was sequentially applied. Surviving weeds including barnyardgrass were finally controlled by mixture of bentazon+quinclorac WP foliage application. 2. In case of direct rice seeding in flooding field, weed control were mostly unsuccessful partially due to wrong selection of herbicide and missing the optimum application time. Three relatively successful weed control in the survey were summarized as follows. 1) Oxadiazon EC, butachlor or benthiocarb were treated just after puddling(5 to 7 days before seeding). then mixture of bentazone+quinclorac WP or sulfonylurea derivatives was sequently applied to control remaining weeds at 20 days after seeding. 2) Mixtures of bensulfuronmethyl+dimepiperate G, pyrazosulfuronethyl+molinate G, or bensulfuronmethyl+mefenacet+dymron G were applied at 11 days after puddling when barnyardgrass were at 2.0 leaf stage. Phytotoxicity was not found in case of mixture of bensulfuronmethyl+dimepiperate G but found in the other two cases but disappeared later. 3) Mixtures of bensulfuronmethyl+quinclorac G., pyrazosulfuronethyl+quinclorac G or betazone and quinclorac G were treated after 18 to 20 days after puddling when barnyardgrass was within 3.0 leaf stage. It showed good weed control in both annuals and perrenials without phytotoxicity. On the contrary, other sulfonylurea derivatives such as middle periodic herbicide showed poor weed control against barnyardgrass, so that sequential treatment of bentazone+quinclorac WP mixture was required. 3. Herbicidal characteristics and optimum application time of 45 rigistered herbicides in Korea were analyzed to discover new substitute for quinclorac mixture, that showed excellent weed control against barnyardgrass at its 3 leaf stage or older. The analysis revealed that 70% of herbicides were for preemergence and the others were post periodic herbicide. Most farmers favor to apply herbicide when rice seedlings completely rooted, at this time barnyardgrass are at 2.5-3.0 leaf stage. Therefore herbicide of which optimum application time had long is required. In this study. 6 middle periodic herbicides among sulfonylurea derivatives and 2 quinclorac mixture were selected and evaluated their weeding spectrums at different leaf stage of barnyardgrass in both soil application in flooding condition and foliage application in dry paddy field. The order of weeding spectrum in magnitude was as follows : bentazone+quinclorac WP> bentazone + quinclorac G>bensulfuronmethyl + quinclorac G>pyrazosulfuronethyl + quinclorac G> pyrazosulfuronethyl + Molinate G>bensulfuronmethyl + mefenacet + dymron G>bensulfuronmethyl + mefenacet G>bensulfuron methyl+benthiocarb G. The above results coincided with that of the survey. In conclusion, there is no proper substitute for quinclorac mixrure, which can control barnyardgrass at 3.0 leaf stage or even older. Therefore quinclorac should be supplied continuously to farmers in order to anchor direct rice seeding in Korea. Author suggested the followings to eastablish direct rice seeding technology effectively and quickly : 1) A tentatively named "The research committee for direct rice seeding" which was composed of farmers. researchers and goberment. should be eastablished to cooperate effectively. 2) Development of a pricise direct rice seeding machine for both dry and flooding paddy field. which is workable regardless of condition and varieties of seeds. 3) Study on protecting rice seed and seedling from sparrows. 4) Systematic studies of weed control techniques in direct rice seeding to standardize herbicide application. 5) Studies on farm-land reformation. techniques of precise land preparation. and direct rice seeding using an airplane.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.4
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• pp.286-291
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• 2003

Monochoria vaginalis is one of the most troublesome resistant weeds in Korean rice culture. Thus, the objectives of this study were to evaluate the response of M. vaginalis resistant to sulfonylurea(SU) herbicides and to determine alternative herbicides for the control of resistant M. vaginalis in direct seeded and transplanted rice culture in Korea. In greenhouse studies, the resistant biotype was 31-, 38-, 3172-, and 7-fold more resistant to ben-sulfuron-methyl, cyclosulfamuron, imazosulfuron, and pyrazosulfuron-ethyl, respectively, than the susceptible biotype, indicating cross-resistance to the SU herbicides used in this study. Non-SU herbicides, butachlor, carfentrazone-ethyl, mefenacet, pretilachlor, pyrazolate, and thiobencarb, several SU herbicide-based mixtures, ethoxysulfuron plus fentrazamide, pyrazosulfuron-ethyl plus pyrazolate plus simetryn, and non-SU herbicide-based mixtures, pyrazolate plus butachlor, pyrazolate plus pretilachlor, simetryn plus molinate, carfentrazone-ethyl plus butachlor, and carfentrazone-ethyl plus thiobencarb can be used to control both the resistant and susceptible biotypes of M. vaginalis when applied before the second leaf stage. In the field experiment, the resistant biotype of M. vaginalis that survived from the paddy fields treated with a SU herbicide-based mixture could effectively be controlled by using mixtures of bentazone plus MCPA, bentazone plus mecoprop-P, and bentazone plus 2,4-D when applied at 2 or 4 main leaves. Our results suggest that the SU-resistant M. vaginalis had not developed multiple resistances to herbicides with different modes of action. In particular, bentazone plus MCPA and bentazone plus mecoprop-P were effective control measures after failure to control resistant M. vaginalis in Korean rice culture.