• 한국동물학회지
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• 제28권4호
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• pp.237-244
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• 1985

非姙娠 女子, 姙娠된 女子 및 正常인 男子의 血漿蛋白質을 SDS/polyacrylamide 젤 電氣泳動으로 分析하였다. 分子量 10,000에서 110,000 dalton 사이의 正常인 男子와 非姙娠 女子의 血漿蛋白質의 패턴은 同一하였다. 本 硏究에서 非姙娠 女子와 姙娠된 女子의 血漿蛋白質을 比較하였을 때 새로운 band들이 나타나지는 않았지만 어떤 蛋白質 band들은 姙娠의 初期, 中期 및 末期에 따라서 量的으로 增加 EH는 減少되었다. 各 band들의 分子量을 測定한 結果 分子量 76,000 dalton 以上의 蛋白質들이 姙娠中 增加 또는 減少되어 짐을 알 수 있었다. 즉 分子量 86,00 dalton의 蛋白質은 姙娠 中期 까지는 增加되지 않았으나 姙娠 末期에는 增加되었다. 分子量 91,000-105,000 dalton 사이의 蛋白質들은 姙娠의 期間이 길어짐에 따라 대체로 量이 增加되었다. 反面에 分子量 94,000 dalton의 蛋白質은 姙娠 中期 까지는 量이 若干 減少 되었으나 末期에는 오히려 增加되었다. 그리고 分子量 99,000 dalton의 蛋白質은 姙娠 初期까지는 變化가 없었으나 中期에서 末期로 감에 따라 漸次的으로 增加되었다. 그러므로 우리들은 姙娠된 女子 血漿의 蛋白質 패턴을 調査함으로써 姙娠의 期間을 알 수 있음을 提示하는 바이다.

• 한국동물학회지
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• 제28권2호
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• pp.61-70
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• 1985

斧足綱 數種의 蛋白質을 電氣泳動한 結果, 새꼬막과 피조개의 血漿의 蛋白質 패턴은 低分子 蛋白質에서 染色되는 强度가 약간 差異가 있으나 거의 同一하였고, 全血球의 蛋白質 패턴의 差異는 17,800 dalton의 band에서 나타났다. 그렇지만 위의 두 種의 筋內의 단백질 패턴은 거의 비슷해서 10,000$\\sim$100,000 dalton의 分子量 範圍內에서는 差異를 알아 내기가 매우 어려웠다. 그런데 대합과 비늘백합은 類緣關係가 있는 蛋白質 패턴이 많이 나타났으며 재첩은 대합이나 비늘백합과 같은 이들 두 種과 대칭이의 中間 程度에 位置하는 蛋白質 패턴을 나타내고 있었다. 본 연구에서는 Bivalvia가 함께 다 갖는 6개의 蛋白質 band들과, 꼬막과 새꼬막에서만 나타나는 4개의 特有한 蛋白質 band가 存在함이 發見되었으며 Eulamellibranchia目의 4종에 있어서 다 나타나는 2개의 protein band들 과, 대합과 비늘백합에서만 나타나는 23,000 dalton의 特有한 band도 發見되었다. 그리고 各 種마다 가지는 特有한 蛋白質 패턴의 分子量을 測定하여 比較하였다.

• 한국식품저장유통학회지
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• 제4권3호
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• pp.301-309
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• 1997

This study was conducted to examine morphological characteristics and the content of cell wall polysaccharides of Brassica campestris var. pekinensis(baechu). First of all, the variety of scientific name and naming of parts of baechu in the literatures of kimchi showed, which will unify marks. So, we propose not so much mid-rib and leaf blade of baechu leaf as white part and green part, respectively. On the other hand, the forms of vessel elements of white part in baechu consist in ring, sclariform and reticulate thickening. The proximate compositions and contents of cell wall polysaccharides of baechu has significant differences between its cultivars. The cell wall pectin from baechu exhibited four peals with molecular weights of 2,000,000, about 100,000 and less than 10,000 by gel filteration chromatography and hemicellulose did two peaks with molecular weights of 2,000,000 and 10,000.

• Journal of Microbiology and Biotechnology
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• 제5권3호
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• pp.177-180
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• 1995

In horseradish peroxidase-catalyzing polymerization of phenol under the water/dioxane solvent system, the optimal concentration of hydrogen peroxide was found to be 10 mmol/I. Feeding of hydrogen peroxide at its optimal concentration improved the polymerization performance by reducing reaction time and increasing molecular weights. Monomer conversions and the molecular weights of the enzymatically produced polymer were in the ranges of 83.1~94.2$%$ and 58000~68000, respectively.

• 한국인쇄학회지
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• 제26권1호
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• pp.1-16
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• 2008

The rheological properties of emulsified ink must be maintained adequately at various shear rates or shear stress. So it is necessary to understand its rheological properties in order to regulate the printability of eumulsified ink on the performance. In this study, the rheological properities and printability of emulsified ink are investigated according to the molecular weights of rosin-modified phenolic resin.

• Macromolecular Research
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• 제15권4호
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• pp.302-307
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• 2007

The living radical polymerization of methyl methacrylate (MMA) by atom transfer radical polymerization, (ATRP) employing a $Fe(II)X_2/diphenyl-2-pyridyl$ phosphine (PyP) catalytic system (X=Cl, Br), was investigated using several initiators and solvents at various temperatures. Most of the polymerizations with the PyP ligand were well controlled, with a linear increase in the number average molecular weights ($M_n$) vs. conversion, with relatively low molecular weight distributions ($M_w/M_n=1.2-1.4$) throughout the reactions. The measured weights matched those of the predicted values. The ethyl-2-bromoisobutyrate (EBriB) initiated ATRP of MMA, with the $Fe(II)X_2/diphenyl-2-pyridyl$ phosphine catalytic system (X=Cl, Br), was better controlled in p-xylene at $80^{\circ}C$ than the other solvents used in this study.

• Preventive Nutrition and Food Science
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• 제4권1호
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• pp.14-17
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• 1999

Bovine hide and pig skin gelatins were prepared and their molecular weight profiles were examined by SDS-PAGE. The major molecular weights of bovine hide gelatin were 220 kDa, 140kDa, and 130kDa and the weights of pigskin gelatin were 210 kDa, 135kDa and 120kDa. Also , as a typical parameter of rheological property of the gelatin , viscosities of gelatin were measured under various conditions. Gelatin hydrolysates were prepared using typical commerical proteases and their angiotensin converting enzyme inhibitory activities were examined.

• Journal of Microbiology and Biotechnology
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• 제14권1호
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• pp.41-47
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• 2004

This study was performed to investigate the antimicrobial effects of hetero-chitosans and their oligosaccharides on the halophilic bacterium, Vibrio parahaemolyticus. Nine classes of hetero-chitosan oligosaccharides were prepared based on their molecular weights, using an ultrafiltration membrane reactor system with chitosanase and celluase, from partially different deacetylated chitosans, 90%, 75%, and 50% deacetylated chitosan, respectively. Thirty-two strains of V. parahaemolyticus were isolated from various marine organisms such as shellfish, shrimps, octopus, and seabirds. Seventy-five percent deacetylated chitosan showed the highest antimicrobial acitivity. The minimal inhibitory concentration (MIC) was 0.5 mg/ml on 14 strains of V. parahaemolyticus, and MIC of the rest strains (18 strains) was 1.0 mg/ml. In addition, MIC of most hetero-chitosan oligosaccharides was 8.0 mg/ml. The results revealed that the antimicrobial effects of hetero-chitosans and their oligosaccharides against V. parahaemolyticus depend on the degree of deacetylation, their molecular weights, and strains tested.

• 약학회지
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• 제27권4호
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• pp.315-320
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• 1983

Ginseng proteins have been extracted and partially purified by the methods of ammonium sulfate fractionation, heat inactivation and Sephadex G-75 column chromatography. The final three fractions obtained (GI, GII and GIII) were subjected to paper chromatography and SDS-polyacrylamide gel electrophoresis. Molecular weights of polypeptide chains from each fraction were est-mated by the standard line obtained from the plot of electrophoretic mobilities against the logarithm of molecular weights of standard proteins. Results are as follows: 1) GI showed three protein spots and four polypeptides of which M.W. were 63,000, 60,000, 56,000, 51,000 and 34,200. 2) GII showed four protein spots and five polypeptides with their M.W. of 64,600, 56,000, 45,400, 35,800, end 25,200, 3) GIII showed three protein spots and four polypeptides with their M.W. of 66,000, 63,000, 56,000 and 22,000.

• 미생물학회지
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• 제25권3호
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• pp.173-179
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• 1987

한국산 A, tumefaciens의 3균주에서 분리한 Ti plasmid의 type과 분자량을 조사한 결과로 이들 모두는 octopine type 이었으며 각 plasmid의 분자량은 pTi12가 44Kb이었고, pTi14는 180Kb이었으며, pTi14는 172Kb이었다. 이중 pTi12를 Smal 및 HindIII로 가수분해한 결과 8개의 Smal digest fragment와 10개의 Hind III digest fragment를 얻었으며 Southern hybridization techniques을 이용하여 잠정적인 physical map을 작성하였다.