• Title/Summary/Keyword: molecular shape

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Molecular phylogeny of Indonesian Zeuzera(Lepidoptera:Cossidae) wood borer moths based on CO I gene sequence

  • Sutrisno, Hari
    • Journal of Species Research
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    • v.4 no.1
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    • pp.49-56
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    • 2015
  • Zeuzera is one of the most important wood borer pests in South East Asia. Like of most other genera of moths, the systematic of this genus is still in dispute, especially on the monophyly and the relationship within this genus due to the fact that genus is very varied. This genus was defined based on external characters only such as cross vein Sc-Rs present, humeral plate approximately triangular in shape and anal plate moderately long to moderately short. Therefore, the monophyly of this genus need to be evaluated based on more comprehensive data. To clarify the monophyly of the genus Zeuzera, to reveal the phylogenetic relationships among the Indonesian species, and to establish the genetic characters of Indonesian Zeuzera, we analyzed seven species of Indonesian Zeuzera including three other species distributed around the world based on nucleotide sequence variation across a 580-bp region in the CO I gene. The results showed that the monophyly of Zeuzera was supported by bootstrap tests at the MP and ML tree building methods (>95%). Genus Zeuzera was divided into two groups (A and B) with Z. borneana was excluded from the two groups and occupied at the basal node. Indonesian species was distributed into two different clades. CO I gene alone was able to fully resolve the relationships among species within clade B. However, further investigations were needed by including more species and other genes that the more conserved to test the validity of the phylogenetic hypothesis proposed here.

Spontaneous Vesicle Formation in Aqueous Mixtures of Cationic Gemini Surfactant and Sodium Lauryl Ether Sulfate

  • Cheon, Ho-Young;Jeong, Noh-Hee;Kim, Hong-Un
    • Bulletin of the Korean Chemical Society
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    • v.26 no.1
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    • pp.107-114
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    • 2005
  • Molecular aggregates of surfactant molecules consisting of one or more bilayers arranged in a hollow, closed, usually spherical geometry are termed “esicles”or “iposomes” In recent years it has been found that in certain systems the vesicular structure forms spontaneously and is long lived, and it has been suggested that these structures may in fact constitute the equilibrium state in these cases (as is true of micelles) This paper deals with the mixed CMC, vesicles, phase behavior, phase transition, geometrical structure, their formation and characterization in the aqueous solutions of mixed cationic/anionic surfactants systems. TEM micrographs revealed that the vesicles were of spherical shape and that their size was of around 180 nm. The zeta potentials are positive at CGS1-rich regions and negative at SLES-rich regions. In the region where SLES/CGS1 (6/4), the zeta potentials are very small, implying that the vesicles at this surfactant ratio may be less stable. At other surfactant ratios, the vesicles are thought to be stable, supported by large absolute values of zeta potentials and little change in UV absorbance for several months.

Simulations of fiber spinning and film blowing based on a molecular/continuum model for flow-induced crystallization

  • McHugh, Anthony J.;Doufas, A.K.
    • Korea-Australia Rheology Journal
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    • v.13 no.1
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    • pp.1-12
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    • 2001
  • This paper describes the application of our recently developed two-phase model for flow-induced crystallization (FIC) to the simulation of fiber spinning and film blowing. 1-D and 2-D simulations of fiber spinning include the combined effects of (FIC), viscoelasticity, filament cooling, air drag, inertia, surface tension and gravity and the process dynamics are modeled from the spinneret to the take-up roll device (below the freeze point). 1-D model fits and predictions are in very good quantitative agreement with high- and low-speed spinline data for both nylon and PET systems. Necking and the associated extensional softening are also predicted. Consistent with experimental observations, the 2-D model also predicts a skin-core structure at low and intermediate spin speeds, with the stress, chain extension and crystallinity being highest at the surface. Film blowing is simulated using a "quasi-cylindrical" approximation for the momentum equations, and simulations include the combined effects of flow-induced crystallization, viscoelasticity, and bubble cooling. The effects of inflation pressure, melt extrusion temperature and take-up ratio on the bubble shape are predicted to be in agreement with experimental observations, and the location of the frost line is predicted naturally as a consequence of flow-induced crystallization. An important feature of our FIC model is the ability to predict stresses at the freeze point in fiber spinning and the frost line in film blowing, both of which are related to the physical and mechanical properties of the final product.l product.

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A Study on the Time-Dependent Deformation Behaviors of PMMA in Nanoindentation Process for Hyperfine Pit Structure Fabrication (극미세 점 구조체 제작을 위한 나노압입 공정에서 PMMA의 시간의존적 변형거동에 관한 연구)

  • Kim Hyun-Il;Kang Chung-Gil;Youn Sung-Won
    • Journal of the Korean Society for Precision Engineering
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    • v.22 no.7 s.172
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    • pp.62-70
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    • 2005
  • The nanoindenter and AFM have been used for nanofabrication, such as nanolithography, nanowriting, and nanopatterning, as well as measurement of mechanical properties and surface topology. Nanoscale indents can be used as cells for molecular electronics and drug delivery, slots for integration into nanodevices, and defects for tailoring the structure and properties. Therefore, it is very important to make indents of desired morphology (shape, size and depth). Indents of different shapes can be obtained by using indenters of different geometries such as a cube comer and conical and spherical tips. The depth and size of indents can be controlled by making indentations at different indentation loads. However, in case of viscoplastic viscoelastic materials such as polymethylmethacrylate (PMMA) the time dependent deformation (TDD) should also be considered. In this study, the effect of process parameters such as loading rate and hold-time at peak load on the indent morphology (maximum penetration depth, elastic recovery, transient creep recovery, residual depth pile-up height) of PMMA were studied for hyperfine pattern fabrication.

Current Status of Bacterial Brown Stripe of Rice Caused by Acidovorax avenae subsp. avenae (Acidovorax avenae subsp. avenae에 의한 세균성줄무늬병의 연구동향)

  • 송완엽
    • Plant Disease and Agriculture
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    • v.5 no.2
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    • pp.69-76
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    • 1999
  • Acidovorax avenae subsp. avenae is the causal pathogen of several hosts including oats corn foxtail millet wheatgrass sugarcane and rice. The pathogen is a seedborne pathogen of rice and known to occur widely in rice growing countries. The pathogen cause inhibition of germination brown stripe on the leaf curling of the leaf sheath and abnormal elongation of the mesocotyl of irce. Bacterial colonies grow slowly and are convex circular and creamy with tan to brown center. The causal baterium is Gram-negative and rod shape with a single polar flagellum Nonfluorescence poly-$\beta$-hydroxybutyrate accumulation and precipitate formation around the colony on the medium are useful in the differentiation of this bacterium from other subspecies of A. avenae as well as nonfluorescent bacteria pathogenic to rice. This bacterium has belonged to the genus of Psdeudomonas but recently was transferred to the new genus Acidovorax on the basis of bacteriological and molecular biological data. However the difference of biochemical characteristics protein profile of the cell and host range among strains should be more clarified. To develop an effective control strategy for this disease understanding of detailed life cycle of the disease ritical environmental factors affecting disease development on each host and relationship to grain discoloration of rice are prerequisite. Although the affected area has been world-widely reported there is on recent progress on the understanding of the bacteriological and ecological characteristics of the causal bacterium and control means of the disease.

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Fatigue Crack Propagation of Sliding Core in Artificial Intervertebral Disc due to the Fatigue Loading Mode (인공추간판의 피로하중 모드에 따른 슬라이딩 코어의 피로균열전파 거동)

  • Kim Cheol-Woong;Kang Bong-Su
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2006.05a
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    • pp.367-368
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    • 2006
  • Today, the Artificial Intervertebral Disc (AID) is being developed by increasing the oblique of the endplate gradually. In other words, Ultra-high Molecular Weight Polyethylene (UHMWPE) which is apply to the sliding core of the AID, does not change the shape but alters the oblique of endplate. However, the unreasonable increase of degree of freedom (DOF) can result in the aggravation of the bone fusion and the initial stability and it can also lead to the increase of the concentrated force in core. For these reasons, it is necessary to develop the advanced techniques, which choose the most adequate DOF. In this study, the new optimized modeling of the sliding core and the endplate, the fatigue characteristics, the crack propagation and the formation mechanism of wearing debris was studied and the minimizing technique will be derived from this research.

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Improvement of floral scent of ornamentals via metabolic engineering (화훼작물의 향기 성분 증대를 위한 대사 공학)

  • Kang, Seung-Won;Seo, Sang-Gyu;Ryu, So-Young;Pak, Chun-Ho;Lee, Gung-Pyo
    • Journal of Plant Biotechnology
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    • v.37 no.1
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    • pp.1-11
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    • 2010
  • Floral scent emitted from many plants is the key factor for pollinator attraction and defense for survival in nature and is important industrial materials for perfumery as well. It is a complex mixture of various organic molecules with a high volatility or a high vapor pressure. In general, floral scents are divided into three categories, aliphatics, terpenoids, and phenylpropanoids/benzenoids, based on its origin. About 1,700 scent compounds have been identified and their biochemistry and molecular biology also have elucidated their biosynthesis from various flowering plants during the last ten years. In addition to improvement of vase life, flower color and shape, and/or disease resistance, floral scent is coming up to the major breeding target for improvement of marketability. Therefore, metabolic engineering can be an important tool in near future and may be able to facilitate the breeding program for novel cultivar selection and improvement of marketability of floricultural crops.

Characterization of Sclerotinia sclerotiorum Isolated from Paprika

  • Jeon, Young-Jae;Kwon, Hyuk-Woo;Nam, Ji-Sun;Kim, Seong-Hwan
    • Mycobiology
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    • v.34 no.3
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    • pp.154-157
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    • 2006
  • A fungal isolate collected from infected paprika (Capsicum annuum var. grossum) was characterized as Sclerotinia sclerotiorum based on its ability of sclerotium formation, physiological and molecular properties. When the isolate was grown on potato dextrose agar, oatmeal agar, and malt extract agar, it grew most well on PDA. Optimal temperature and pH for its growth were $25^{\circ}C$ and pH 7, respectively. The fungal isolate produced sclerotia on PDA within 10 days, and the color and shape of the sclerotia were similar to those of S. sclerotiorum. The ITS rDNA regions including ITS1 and ITS2 and 5.8S sequences were amplified using ITS1F and ITS4 primers from the genomic DNAs of the paprika isolate and other known pathogenic S. sclerotiorum isolated from different crops in Korea, and their nucleotide sequences were determined. Sequence comparison analysis showed the ITS rDNA of the paprika isolate shares 100% sequence identity with those of S. sclerotiorum isolated from red pepper, lettuce and a S. sclerotiorum isolate registered in GenBank DNA database. Neighbor joining analysis based on the ITS rDNA sequence revealed the paprika isolate has very close phylogenetic relationships with known Sclerotinia sclerotiorum isolates. This is the first report that S. sclerotiorum has been found associated with paprika rot in paprika growing countries.

DFT 방법을 이용한 벤젠 삼합체 π-π interaction의 양자역학 계산

  • Jeong, Hyeon-Su;Park, Gi-Cheol;Cho, Art.
    • Proceeding of EDISON Challenge
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    • 2014.03a
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    • pp.399-408
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    • 2014
  • 신약을 개발하거나 단백질 구조를 예측하는데 Molecular Mechanics (MM)의 방법을 사용한다. 하지만, MM 만으로는 자연현상에서 일어나는 결과를 정확하게 기술하기 어렵다. 본 연구는 기존의 MM 방법으로는 정확히 예측이 불가능한 비 공유결합 중 하나인 ${\pi}-{\pi}$ interaction을 양자역학 계산을 통해 정확한 예측이 가능한지 알아보았다. ${\pi}-{\pi}$ interaction이란 생채 내, 의약 화합물에서 발견되는 결합이기 때문에, 단백질과 결합하는 구조의 예측에 중요하다고 할 수 있다. 본 실험은 ${\pi}-{\pi}$ interaction을 갖는 Sandwich, T shape, 그리고 Parallel displaced 세 가지 모형과 각각의 모형 아래에 분자를 하나 더 쌓은 모형을 추가하여 양자역학 재산을 수행하였다. 양자역학 계산은 DFT의 세가지 함수 M06_2X, M05_2X, B3LYP를 이용하였다. 실험결과에서 세 가지 함수가 각기 다른 결과를 보였는데, 상대적으로 B3LYP의 경우에는 세가지 모델에서 모두 제대로 된 에너지 변화를 계산하지 못하였으며, M06_2X와 M05_2X의 결과에서는 거리에 따른 ${\pi}-{\pi}$ interaction 에너지의 변화를 정확하게 계산하였다. 이러한 결과를 바탕으로, 양자역학의 방법을 통해 MM에서는 예측이 불가능한 ${\pi}-{\pi}$ interaction을 계산 할 수 있고 이 부분을 고려하여 화합물 간의 결합구조를 예측을 향상시킬 수 있다.

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Temperature and Pressure Dependence of the Nuclear Magnetic Relaxation Rates of -$NH_2$ Group in Thioacetamide / Acetone Solutions (티오아세트아미드 / 아세톤 용액의 아민기의 핵자기이완속도의 온도 및 압력 의존)

  • Kim Keon;Choi Young Sang;Yoon Chang Ju;E. Lang
    • Journal of the Korean Chemical Society
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    • v.36 no.1
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    • pp.33-37
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    • 1992
  • Information about $^{14}N$ relaxation rates is obtained for thioacetamide (TA) in acetone from a detailed line-shape analysis of the $^1H$ resonance signal of $NH_2$ group. The effect of pressure and of temperature were investigated, and activation energies for molecular reorientation are given. And also $^1H$ relaxation rates of $NH_2$ group of TA as a function of pressure and temperature are obtained. These results are discussed with the results for the $^{14}N$ relaxation of amine group.

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