• Korean Journal of Computational Design and Engineering
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• v.9 no.2
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• pp.112-121
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• 2004

As customers demands are rapidly changing, a product life cycle is getting shorter and a product model is forced to be changed frequently. An ejecting design system becomes more important for high productivity to eject a product in high temperature without any damage. For example, an ejector pin that is a key component of the system can cause high local stresses and strains in the molding at the time of ejection. The number, the size, and the location of pins are important to make a smooth ejection. Therefore we propose an analytical approach with the aid of designer’s experience to calculate a total release force and pressure distribution so that the number, the size, and the location of pins can be easily determined. As a part of the result, the design system is built by Intent! with AutoCAD 2000 and a video player deck example is presented to verify the approach.

• Journal of the Korean Home Economics Association
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• v.29 no.2
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• pp.47-53
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• 1991

A. niger H-18 and A. fumigatus E-29 wrer selected for their strong abilities to produce cellulase. The λd numerical values of the cotton fabrics inoculated with A. niger H-18 and A. fumigatus E-29 were 580 nm for the both strains of molds. By the growth of molds, lightness, original color scale, and grey scale of the fabrics gradually decreased while chroma increased. The minimum inhibitory concentrations of mold-proofing agents, such as Leperon WL, 8-hydroxyquinoline copper acetate, trimethylol melamine and dimethyl ethylene urea was 50 ppm. Glycoxale was not effective at the above mentioned concentration. Since Leperon WL, trimethylol melamine and dimethyl ethylene urea effectively inhibited the growth of A. niger H-18 and A. fumigatus E-29, tensile strength and elongation of the fabrics were not changed. however, cotton fabrics treated with glycoxale of the fabrics were not changed. However, cotton fabrics treated with glycoxale and inoculated with A. niger H-18 and A. fumigatus E-29 showed decreased in tensile strength by 31.1% and 33.9%, respectively.

• Food Science and Biotechnology
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• v.18 no.4
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• pp.1030-1034
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• 2009

A fermenting microorganism involved in formation of ortho-dihydroxyisoflavones (ODIs) during aging of doenjang (Korean fermented soybean paste) has been investigated. Microorganisms in ODI-containing doenjang were isolated by cultivating on yeast mold (YM) agar medium containing 0-7% NaCl. ODI formation of the isolated strains was examined by gas chromatography/mass spectrometry (GC/MS) analysis after cultivation in modified YM broth or soybean extract medium. An ODI-producing microbe was identified as Bacillus subtilis HS-1 based on 16S rRNA gene sequence analysis. The strain has produced 8-hydroxydaidzein as a major product during growth in the modified YM broth or soybean extract medium. Therefore, it was concluded that one of the microorganisms involved in the formation of ODIs in doenjang was B. subtilis HS-1.

• Microbiology and Biotechnology Letters
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• v.25 no.4
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• pp.396-402
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• 1997

Biocontrol of plant pathogens provides an alternative means of reducing the incidence of plant diseases without the negative aspects of chemical pesticides. Nowdays, as the resistant fungi about the chemical fungicides have revealed and the concern of environment has increased, the biological control of phytopathogenic fungi by the antagonistic microorganisms is very much indispensable. For the selection of strong antagonistic bacterium for biological control agent of rice leafblast and cucumber gray mold rot, the antifungal strain KL-3 strain was selected among 120 strains isolated from the rhizosphere soils. And the strain was identified to be a species of Bacillus subtilis or closely related strain. In several biochemical and in vitro antibiosis tests, antifungal substances of Bacillus sp. KL-3 were presumed heat stable, micromolecular antibiotic substances. In vivo test and vinyl house field test, the antifungal substances of Bacillus sp. KL-3 represented excellent biocontrol ability aganist Alternaria mali, Phyricularia oryzae, and Alternaria kikuchiana as well as broad spectrum of other fungi. In particular, Bacillus sp. KL-3 strain showed more predominant activity than some chemical fungicides against fungi shown to resist chemcal fungicides.

• Journal of Ginseng Research
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• v.3 no.2
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• pp.144-155
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• 1979

In order to investigate biochemical effects of ginseng saponin on microbial enzyme activity, Aspergillus oryxae-143 and Aspergillus niger-40, which were selected from various sources of samples and were the highest enzyme producing mold strains, were grown in the medium containing various saponin concentration (0mg%, l0mg%, 50mg%, l00mg%, 150mg% and 300mg%). The enzyme activity (amylase, protease) was found most active when the saponin was added in the culture media with the concentration of l0mg%∼l00mg%. But it seemed that the action of microbial enzyme was inhibited by adding more than 300mg% of saponin.

• Proceedings of the Korean Geotechical Society Conference
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• 2001.03a
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• pp.33-40
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• 2001

In this research, the cracking of tunnel concrete lining was investigated and analyzed through long-term measurement and nonlinear numerical analysis. For one year after the casting of lining, the stresses and strains were measured by the sensors installed in hard rock tunnel lining. The measurements showed that only small stresses which were less than cracking stress occurred in every survey sections regardless of sensor directions. It could be induced that the external load applied to the lining was small or ignorable. Also, it was carried out short-term numerical analysis based on such site condition as ambient temperature, the- degree of overbreak and mold staying period. Long-term numerical analysis based on creep & shrinkage and nonlinear cracking was carried out. The output showed that construction condition and ambient environments could make the lining concrete crack without external loads. The cracks formed in this process does not indicate the structural instability of the tunnel.

• Journal of Food Hygiene and Safety
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• v.14 no.2
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• pp.167-171
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• 1999

In order to screen the antagonistic bacteria which inhibit the growth of the apple pathogen, Penicillum expansum, we isolated an effective bacterial strain and investigated into the antifungal activity of the antagonist and it's identification. The eleven strains of bacteria which strongly inhibited P. expansum were isolated from the nature, and the best antagonistic bacterial strain designated as CH142, was selected. The antagonistic strain CH142 was identified to be the genus Bacillus subtilis based on morphological and biochemical characterization. The CH142 showed 55.9% of antifungal activity against the growth of P. expansum. By the treatment of the culture broth and the heat treated culture filtrate of it, the B. subtilis CH142 showed 90% and 15% of antifungal activity, respectively.

• Korean Journal of Microbiology
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• v.7 no.1
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• pp.41-44
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• 1969

A new method for manufacturing the active seed starter (Korean Kok-Ja) has been studied, designed new method consists of the boiling process of raw wheat material and pure culture process of Rhizopus fungi. The feasibility study for industrialization of these methods have been performed, the liquefying and saccharifying activities were put to analysis and compared with conventional product of Kok-Ja. The isolated strains of mold from raw wht and Kok-Ja were idetified as Rhizopus sp., Aspergillus sp., Aspergillus niger sp., Endomyces sp., and Fungi imperfecii. It is proved that the saccharifying and liquefying activities are much stronger than that of conventional product of Kok-Ja, and the growth of Rhizopus strain in boild what material are vigorous than that of old type Kok-Ja. Then it is considered the newly designed method is effective to manufacturing a fine better Kok-Ja.

• Applied Biological Chemistry
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• v.13 no.3
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• pp.237-242
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• 1970

Fifty strains of mold which isolated from the various sources were screened for the production of Naringinase which hydrolyse naringin, the 7-rhamnoe-glucoside of 4'.5.7. - trihydroxyflavanonin, the main bitter principle of citrus fruits and grape fruits. Of the 4 strains yielded naringinase with significant activity, S-1 strain was selected on the criterion of industrial application, and some properties of crude naringinase of this S-1 was investigated. The results obtained were as follows. 1. Naringenase obtained from S-1 strain has optimum pH range from 3.0 to 5.0 for its activity. 2. Production of naringinase was increased on the addition of naringin to the medium. 3. Hydrolysis of naringin with approporiate concentration of naringinase was carried out linerly up to 80% on the 0.1% substrate solution. 4. The optimum temperature for its activity was $50^{\circ}C$, and this enzyme was inactivated 80% of its total activity at $70^{\circ}C$ for 10 minutes, 40% at $60^{\circ}C$ for 30 minutes. But signifiant decrease of activity were not occurred by heat treatment at $50^{\circ}C$ for 2 hours. 5. Crude enzyme of the naringinase obtained from S-1 strain was competitively inhibited by addition of glucose on the substrate, and inhibitor constant of the glucose on the this enzyme was 1.5 Mol, and inhibition rate were linearly increased according to the increase of sucrose concentration and 56% of its total activity was inhibited at 1 Mol sucrose solution.

• Applied Biological Chemistry
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• v.8
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• pp.45-50
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• 1967

Starch was liquefied by the bacteriological ${\alpha}-amylase$ and saccharified separately by four strains of mold as saccharogenic enzymes, then thise was fermented for research the fermentation rates, the usefulness of application of four strains of mold and the S.P to need in proportion increasing of concentration of mixed-starches for brewing in this experiment. The obtained results were as follows: 1) In the ability of saccharificatien, fermentation and acid resistance, Aspergillus usamii mut shirousamii, which were used to produce the koji, were excellent, however, Rhizopus delemar had heavy pollution in a lot of production of koji, were-as its qualities as enzyme was good. 2) The necessary S.P in proportion to the concentration of mixed-starch for brewing was found in this experiment in which was fermented in the various concentration of the adding S.P and the mixed-starches for brewing. 3) In the experiment of almost manufacture scale, the S.P needed essentially in the saccharogenic formentation was found and the usefulness of the necessary S.P was observed, and in fact in the manufacture scale it was safer to add the S.P more than 1.2% because it must be considered that the ability of enzymes is reduced by heat or acid. 4) The period of fermentation was shorten because the conditions for yeast propagation were excellent as a results of starches being liquefied by bacteriological amylase, and saccharified by molds as saccharogenic enzymes.