• 생명과학회지
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• 제14권3호
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• pp.391-395
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• 2004

B. stearothermophilus NO2의 CGTase 유전자 (cgtS)를 구성적 $P_{JH}$ promoter 하류에 subcloning 하여 재조합 plasmid pIH-CGT1 (8.14 kb)을 구축하고 B. subtilis DB431에 형질 전환하였다. B. subtilis DB431/pJH-CGT1를 5가지 배지(LB, 2${\times}$LB, 5% molasses+2% CSL, CS, LBG)로 flask 배양하여 균체증식과 CGTase발현량 및 분비국재성을 조사하여 최적 배지를 결정하였다. 그 중 〔5% molasses+2% CSL〕 배지에서 9시간에 1.8 unit/$m\ell$의 CGTase가 발현$.$생산되었다. 이 결과를 토대로 3. subtilis DB431/pJH-CGT1를 〔10% molasses + 5% corn steep liquor〕 배지에서 발효조 회분 배양한 결과, 30시간 배양시 CGTase의 최대 발현량은 4.2 unit/$m\ell$, 90%의 분비 효율, 90% 이상의 plasmid 안정성을 나타내었다. 저렴한 산업용 molasses 배지로 발효조 회분배양시 플라스크 배양보다 균체증식과 CGTase 발현량이 2배 이상의 증가된 값을 얻었다.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1258-1258
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• 2001

Estimation of sugar and reducing sugar content in molasses is very important task in sugar refineries. Conventional methods of determination of sugar content in molasses samples are highly time consuming and employ hazardous chemicals. Due to the physical properties of molasses, probability of error in conventional analytical techniques is high. These methods have proven to be inefficient for a process control in any sugar industry. Hence development of a rapid, inexpensive, physical and also accurate method for sugar determination in molasses will be highly useful. Near Infrared spectroscopy is being widely used worldwide as an analytical technique in food industry. The technique offers the advantage of being non-destructive and rapid. The present paper highlights the potential of near infrared reflectance spectroscopy as a rapid and automated analytical technique for determination of sugar and reducing sugar content in molasses. A number of molasses samples were collected during and after the sugar season from Havana Sugar Industry, Havana. The samples were chosen so as to obtain a wide range of concentration of sugar and reducing sugars. This was done in order to achieve a good calibration curve with widely spread data points. These samples were scanned in the region of 1100 - 2500 nm in diffuse reflectance mode. An indigenous ELICO NIR spectrophotometer, modified according to the requirements of sugar industry was used for this purpose. Each sample was also analyzed simultaneously by standard chemical methods. Chemical values were taken as reference for near infrared analysis. In order to obtain the most accurate calibration for the set of samples, various mathematical treatments were employed. Partial Least Square method was found to be most suitable for the analysis. A comparison is made between the actual values (chemical values) and the predicted values (NIR values). The actual values agree very well with the predicted values showing the accuracy of the technique. The validity of the technique is checked by predicting the concentration of sugar in unknown molasses samples using the calibration curve. The present investigation assesses the feasibility of the technique for on-line monitoring of sugars present in molasses in sugar industries.

• Asian-Australasian Journal of Animal Sciences
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• 제8권3호
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• pp.289-293
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• 1995

The experiment was conducted to study the effect of supplementary urea molasses block lick with rice straw based diet on the performance of sheep. Six indigenous sheep of about two years of age with an average body weight of 12.88 kg, were selected for this experiment They grouped into two by stratified randomization, and the experiment was conducted for a period of 90 days. Sheep of group A was fed rice straw and group B was feed rice straw with urea molasses block lick, beside this both the groups received 66 g wheat bran and 167 g of Ipil-Ipil leaf meat. The study revealed that the average daily gain of live weight per sheep per day was 41 gm and 70 gm in group-A and group-B respectively. From the analysis of variance it was evident that live weight gain in sheep of group B, supplemented with urea molasses block lick was highly significant (p < 0.01). It was also estimated that group A required 8.12 kg DM to gain 1 kg live weight, whereas group B receiving urea molasses block lick required 5.30 kg DM to gain 1 kg live weight. Therefore, feeding rice straw with urea molasses block lick able to utilize more crop-residues efficiently.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권4호
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• pp.306-312
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• 2006

Two culture modes, continuous and semi-continuous, of the decolorization fungus, Geotrichum candidum Dec 1, were compared to obtain a high treatment efficiency of molasses decolorization and a large productivity of peroxidase (DyP) to simultaneously decolorize dyes and molasses. The continuous culture of G. candidum Dec 1 using a 5-I jar-fermentor showed high DyP activity at a low dilution ratio of $0.005h^{-1}$, and decolorization ratio of molasses of 80% was obtained concomitantly. Therefore, a semi-continuous culture was performed by repeated refill and draw. In this mode, approximately 1.5 liters of the culture broth was replaced per cycle when the decolorization ratio of molasses was near 80%. The molasses medium (1.0 liter per day) was treated and the peroxidase productiveity in the drawn culture broth was 26.6U/day, whereas the peroxidase productiveity was 17.9U/day in the continuous culture with a dilution rate of $0.005h^{-1}$. The semi-continuous treatment system was an efficient decolorization method for the strain, G. candidum Dec 1.

• 한국미생물·생명공학회지
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• 제26권2호
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• pp.96-101
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• 1998

여러 가지 외부 탄소원(acetic acid, glucose, methanol, molasses)으로 활성 슬러지를 이용하여 탈질 효과를 살펴보았다. 질산성 질소와 아질산성 질소를 질소원으로 하는 혐기성 슬러지의 회분식 배양을 통해 탈질속도, COD 소비, 탈질율을 조사해 본 결과 molasses는 탈질속도나 COD 소비에서 glucose와 비슷하고 acetic acid나 methanol보다 높았으나, 탈질 효율에 있어서는 질산성 질소나 아질산성 질소배지에서 30-40%가 암모니아로 축적되어 탈질 효율이 떨어졌다. 이러한 현상은 molasses와 glucose를 탄소원으로 이용한 경우 발효에 의해 pH저하를 초래하고 이로 인해 탈질 효율이 감소되는 것으로 생각된다. 따라서 초기 pH를 조절하고 외부 탄소원은 molasses를 이용, 탈질 효과를 조사한 결과 pH 8일 때 암모니아의 축적은 거의 없었으며 탈질 속도에 있어서도 acetic acid의 경우보다 12시간정도 빠른 24시간 안에 99% 이상 탈질 효율이 증가함을 알 수 있었다.

• Journal of Animal Science and Technology
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• 제46권1호
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• pp.61-68
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• 2004

본 연구는 monosodium glutamate의 생산 후 발생되는 부산물인 condensed molasses solubles(CMS)가 반추가축의 질소 공급원으로서 반추위 미생물 단백질 합성에 미치는 효과를 조사하기 위하여 수행하였다. 반추위 canulae가 부착된 4마리의 비착유소를 4 ${\times}$ 4 라틴 방각법에 적용하여 실험을 수행하였으며, 4개의 처리구는 다음과 같다. (1) 기초사료(앞착된 보리 3kg/일과 보리짚의 자유채식), (2) 기초사료에 당밀 200g/일 그리고 물 300g/일 첨가, (3) 기초사료에 당밀 200g/일, CMS 100g/일 그리고 물 200g/일 첨가 (4) 기초 사료에 당밀 200g/일, CMS 200g/일, 그리고 물 100g/일 첨가. CMS의 첨가수준에 따른 반추위내 발효양상은 처리구간에 유의적인 차이가 없었으나, CMS를 200g/일을 첨가한 처리구가 다른 처리구들에 비해 반추위내 미생물 단백질 합성량의 표시로서 이용된 allantoin/creatinine의 비율을 증가시키는 경향이 나타났다(P < 0.10).

• 폴리머
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• 제34권6호
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• pp.522-526
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• 2010

Bacterial cellulose는 초산균인 Gluconacetobacter xylinus에 의해 생산되며, 배양 배지의 표면에 나노섬유상의 막을 형성한다. 본 연구에서는 배지의 조성에서 탄소원을 달리하여 생산한 bacterial cellulose의 결정화도, 점도, 모폴로지와 역학적 물성을 살펴보았다. Gluconacetobacter sp. V6 균은 세 종류의 배양 배지에서 정치 상태로 배양되었다. 배양 배지로는 표준 Hestrin-Schramm 배지와 탄소원으로 glycerol 또는 molasses를 첨가한 개질 배지가 각각 사용되었다. 세포 성장과 셀룰로오스 수율은 molasses 배지와 glycerol 배지에서 증가하였다. Glycerol 배지를 사용한 배양은 결정화도와 고유점도, 파단응력과 같은 셀룰로오스의 물성을 향상시켰으나, molasses 배지를 사용한 배양은 셀룰로오스의 결정화도, 미결정의 크기, 고유점도를 감소시켰다. 요약하면, molasses 배지에서 셀룰로오스의 수율은 현저히 향상되었으나, 낮은 구조적 물성을 가졌다.

• Asian-Australasian Journal of Animal Sciences
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• 제20권6호
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• pp.887-893
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• 2007

Influences of forage DM and addition of cane molasses on silage characteristics of berseem (Trifolium alexandrium) and lucerne (Medicago sativa) and their ruminal digestion kinetics in Nili buffaloes were studied. Berseem and lucerne fodders (at one tenth bloom) were ensiled with wheat straw in laboratory silos to achieve 20, 30, 40% forage DM and without wheat straw (control); each forage DM level was supplemented with 2, 4 and 6% of cane molasses at ensiling. The pH and lactic acid contents of berseem and lucerne silages were affected by both forage DM and addition of molasses. Dry matter, CP and true protein (TP) of berseem and lucerne silages were affected by forage DM at ensiling but were not affected by the addition of cane molasses. Higher DM, CP and TP losses were observed when berseem and lucerne fodders were ensiled either without wheat straw or with wheat straw to achieve 20% and 40% forage DM at ensiling compared with 30% DM at ensiling. Fiber fractions (NDF, ADF, hemicellulose and cellulose) of berseem silage and lucerne silage were significantly increased with increasing forage DM at ensiling. Addition of cane molasses did not affect the DM, CP, TP and fiber fractions of both berseem and lucerne silages. Berseem and lucerne ensiled at 30% DM with 2% cane molasses were screened for comparative ruminal digestion kinetics with their respective fodders. Addition of wheat straw to berseem or lucerne fodder at ensiling depressed DM and NDF ruminal degradability. However, ruminal lag time, rate of degradation and extent of digestion of silages were similar to their respective fodders. In conclusion, berseem and lucerne could be ensiled with wheat straw to increase their DM to 30% along with 2% molasses for buffaloes.

• Bulletin of the Korean Chemical Society
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• 제35권9호
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• pp.2711-2716
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• 2014

The high content of potassium in molasses limits its usage as a raw material for stock feed. Moreover, its high viscosity makes it difficult to develop an efficient removal process. In this study, ion exchange and solvent extraction experiments have been performed to investigate the removal of potassium from a mixture of molasses with water. Cationic exchange resins (AG50W-X8 and Diphonix) showed a high loading percentage of potassium but the occurrence of breakthrough in few bed volumes was a drawback to the industrial application. Among the cationic extractants (D2EHPA, PC 88A, Cyanex 272) tested in this study, saponified PC 88A was found to be the best extractant for the removal of potassium. Batch simulation studies on a three stage counter current extraction confirmed that 85% of potassium was removed from 50 wt % molasses solution in water by using saponifed PC 88A.

• Asian-Australasian Journal of Animal Sciences
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• 제4권4호
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• pp.377-382
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• 1991

The effect of molasses addition and hot temperature on the ensiling characteristics of napier grass (Pennisetum purpureum Schum.) were studied. Napier grass was harvested five times at intervals from 22 to 39 days and each harvest was divided into two equal portions. The half portion was ensiled directly and the other half was ensiled after mixing with molasses into polyethylene bag silos of 15 kg capacity. Molasses was added at the rate of 4% of fresh weight of the grass. One half of the each treatment was conserved at a room of $40^{\circ}C$ for a month and then moved to an ambient temperature room. The other half was kept at ambient temperature for the whole experimental duration. The silages were opened 3 to 7 months after ensiling. Addition of molasses enhanced lactic acid fermentation by increasing lactic acid content and reducing pH value, ammonia nitrogen and acetic, propionic and butyric acid contents of the silages in both temperature treatments. Enhanced temperature increased pH value and decreased acetic, propionic and butyric acids.