• Title/Summary/Keyword: modified form

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Chemical Modification of Lysine Residues in Bacillus licheniformis α-Amylase: Conversion of an Endo- to an Exo-type Enzyme

  • Habibi, Azadeh Ebrahim;Khajeh, Khosro;Nemat-Gorgani, Mohsen
    • BMB Reports
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    • v.37 no.6
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    • pp.642-647
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    • 2004
  • The lysine residues of Bacillus licheniformis $\alpha$-amylase (BLA) were chemically modified using citraconic anhydride or succinic anhydride. Modification caused fundamental changes in the enzymes specificity, as indicated by a dramatic increase in maltosidase and a reduction in amylase activity. These changes in substrate specificity were found to coincide with a change in the cleavage pattern of the substrates and with a conversion of the native endo- form of the enzyme to a modified exo- form. Progressive increases in the productions of $\rho$-nitrophenol or glucose, when para nitrophenyl-maltoheptaoside or soluble starch, respectively, was used as substrate, were observed upon modification. The described changes were affected by the size of incorporated modified reagent: citraconic anhydride was more effective than succinic anhydride. Reasons for the observed changes are discussed and reasons for the effectivenesses of chemical modifications for tailoring enzyme specificities are suggested.

Exact mathematical solution for free vibration of thick laminated plates

  • Dalir, Mohammad Asadi;Shooshtari, Alireza
    • Structural Engineering and Mechanics
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    • v.56 no.5
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    • pp.835-854
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    • 2015
  • In this paper, the modified form of shear deformation plate theories is proposed. First, the displacement field geometry of classical and the first order shear deformation theories are compared with each other. Using this comparison shows that there is a kinematic relation among independent variables of the first order shear deformation theory. So, the modified forms of rotation functions in shear deformation theories are proposed. Governing equations for rectangular and circular thick laminated plates, having been analyzed numerically so far, are solved by method of separation of variables. Natural frequencies and mode shapes of the plate are determined. The results of the present method are compared with those of previously published papers with good agreement obtained. Efficiency, simplicity and excellent results of this method are extensible to a wide range of similar problems. Accurate solution for governing equations of thick composite plates has been made possible for the first time.

Evaluation Methods on ONDOL Thermal Environmental Index (온돌 온열환경지표 평가방법)

  • Kim, Sung-Jo
    • Journal of the Korean Society of Industry Convergence
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    • v.25 no.1
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    • pp.101-110
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    • 2022
  • For this purpose, the authors proposed and proved usefulness of the modified mean skin temperature which is integrated mean radiation temperature and the effect of floor contacted heat conduction. The mean radiation temperature is applied form factor between half cross-legged human body and surrounding wall of indoor. In addition the floor contacted heat conduction is applied heat transfer coefficient of half cross-legged human body. Eight Korean young men were targeted for the experiment. From the experiment the authors excerpted physiological reaction and psychological reaction in Ondol environment which is combined physiccal environmental factor of artificial climate chamber, air and floor temperature. As a result of the experiment it is confirmed that heat conduction has more impact than heat exchange from existing research for the heat exchange between half cross-legged human body and surrounding wall in Ondol thermal environment. Thereby, it is proved the effectiveness of the modified mean skin temperature which is added floor contacted temperature to the Ondol thermal environmental evaluation index.

Adsorption Characteristics of Lithium Ion by Zeolite Modified in K+, Na+, Mg2+, Ca2+, and Al3+ Forms (양이온 K+, Na+, Mg2+, Ca2+, Al3+ 형태로 개질한 제올라이트에 의한 리튬 이온의 흡착 특성)

  • Park, Jeong-Min;Kam, Sang-Kyu;Lee, Min-Gyu
    • Journal of Environmental Science International
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    • v.22 no.12
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    • pp.1651-1660
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    • 2013
  • The adsorption of lithium ion onto zeolite was investigated depending on contact time, initial concentration, cation forms, pH, and adsorption isotherms by employing batch adsorption experiment. The zeolite was converted into different forms such $K^+$, $Na^+$, $Mg^{2+}$, $Ca^{2+}$, and $Al^{3+}$. The zeolite had the higher adsorption capacity of lithium ion in $K^+$ form followed by $Na^+$, $Ca^{2+}$, $Mg^{2+}$, and $Al^{3+}$ forms, which was in accordance with their elctronegativities. The lithium ion adsorption was explained using the Langmuir, Freundlich, and Dubinin-Radushkevich adsorption isotherms and kinetic models. Adsorption rate of lithium ion by zeolite modified in $K^+$ form was controlled by pseudo-second-order and particle diffusion kinetic models. The maximum adsorption capacity obtained from Langmuir isotherm was 17.0 mg/g for zeolite modified in $K^+$ form. The solution pH influenced significantly the lithium ions adsorption capacity and best results were obtained at pH 5-10.

HRTEM Analysis of Apatite Formed on Bioactive Titanium in Modified-SBF (수정된 유사체액 내에서 티타늄에 생성된 아파타이트의 고분해능 전자현미경에 의한 분석)

  • Kim, Hyun-Ook;Kim, Woo-Jeong;Lee, Kap-Ho;Hon, Sun-Ig
    • Korean Journal of Materials Research
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    • v.17 no.8
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    • pp.408-413
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    • 2007
  • Process of the hydroxyapapite(HA) precipitation on bioactive titanium metal prepared by NaOH in a modified-simulated body fluid(mSBF) was investigated by high resolution transmission electron microscope (HRTEM) attached with energy dispersive X-ray spectrometer(EDX). The amorphous titanate phase on titanium surface is form by NaOH treatment and an amorphous titanate incorporated calcium and phosphate ions in the liquid to form an amorphous calcium phosphate. With increasing of soaking time in the liquid, the HA particles are observed in amorphous calcium phosphate phase with a Ca/P atomic ratio of I.30. The octacalcium phosphate (OCP) structure is not detected in HRTEM image and electron diffraction pattern. After a long soaking time, the HA particles grow as needle-like shape on titanium surface and a large particle-like aggregates of needle-like substance were observed to form on titanium surface within needle-like shape. A long axis of needle parallels to c-direction of the hexagonal HA structure.

Effect of Alloying Element Addition on the Microstructure and Wear Properties of Die-casting ADC12 Alloy (ADC12 다이캐스팅 합금의 미세조직 및 기계적 특성에 미치는 개량 원소 첨가의 영향)

  • Kang, Y.J.;Yoon, S.I.;Kim, D.H.;Lee, K.A.
    • Transactions of Materials Processing
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    • v.28 no.1
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    • pp.34-42
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    • 2019
  • In this study, various alloying elements (Cr, Sr, Ca, Cd) were added to improve the mechanical properties of ADC12 fabricated by a die casting process. The effect of alloying elements on the microstructure and mechanical properties were investigated. The phase analysis results of the modified ADC12 alloy with conventional ADC12 alloy, showed the similar characteristics of Al matrix, Si phase, $CuAl_2$ phase and the Fe intermetallic phase. As a result of the microstructure observation, the secondary dendrite arm spacing (SDAS) was shown to have decreased after the addition of the alloying elements. The eutectic Si phase, which existed as flake form in the conventional ADC12 alloy, was modified finely as a fiber form in the modified ADC12 alloy. It was observed that the $CuAl_2$ phase as the strengthening phase was relatively finely distributed in the modified ADC12 alloy. The Fe intermetallic appeared as a Chinese script shaped $Al_6$ (Mn,Fe) which is detrimental to mechanical properties in conventional ADC12 alloy. On the other hand, in the modified ADC12 alloy, polyhedral ${\alpha}-Al_{15}Si_2$ $(Fe,Mn,Cr)_3$ was observed. The tensile properties were improved in the modified ADC12 alloy. The yield strength and tensile strength increased by 12.4% and 10.0%, respectively, in the modified ADC12 alloy, and the elongation was also seen to have been increased. As a result of the pin on disk wear test, the wear resistance properties were also improved by up to about 7% in the modified ADC12 alloy. It is noted that the wear deformation microstructures were also observed, and it was found that the fine eutectic Si and strengthening phases greatly improved abrasion resistance.

Study of Hull Form Development of 5-Ton-Class Catamaran-Type Coastal Fishing Boat for Welfare Accommodation of Fishing Crew (어선원 복지공간 확보를 위한 5톤급 쌍동형 연안어선 선형개발에 관한 연구)

  • Jeong, Uh-Cheul;Kim, Hyun-Soo;Kwon, Soo-Yeon;Choi, Ji-Hoon
    • Journal of Ocean Engineering and Technology
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    • v.29 no.6
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    • pp.405-410
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    • 2015
  • This paper reports the hull form development and resistance performance of a 5-ton-class catamaran-type coastal fishing boat. The weight estimation and preliminary design were basically extracted from existing coastal fishing vessels. In addition, the resistance performance was investigated using a model test in a high-speed circulating water channel and was analyzed in comparison to an existing catamaran fishing vessel. As a result, the modified hull achieved an approximately 30% reduction in resistance compared to the previous hull. The stability or boarding sensitivity of the modified hull form was more stable or comfortable than the original hull form based on a trim and sinkage comparison between the two boats.

On the Modified Supplementary Variable Technique for the Discrete-Time GI/G/1/K Queue (이산시간 GI/G/1/K 대기행렬에 대한 수정부가변수법)

  • Chae, Kyung-Chul;Lee, Doo-Ho;Kim, Nam-Ki
    • Journal of the Korean Operations Research and Management Science Society
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    • v.33 no.1
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    • pp.107-115
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    • 2008
  • We consider the discrete-time GI/G/1/K queue under the early arrival system. Using a modified supplementary variable technique(SVT), we obtain the distribution of the steady-state queue length. Unlike the conventional SVT, the modified SVT yields transform-free results in such a form that a simple two-moment approximation scheme can be easily established.

ON THE GENERALIZED MODIFIED k-BESSEL FUNCTIONS OF THE FIRST KIND

  • Nisar, Kottakkaran Sooppy
    • Communications of the Korean Mathematical Society
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    • v.32 no.4
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    • pp.909-914
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    • 2017
  • The recent research investigates the generalization of Bessel function in different forms as its usefulness in various fields of applied sciences. In this paper, we introduce a new modified form of k-Bessel functions called the generalized modified k-Bessel functions and established some of its properties.

Analytical solution of stress-strain relationship of modified Cam clay in undrained shear

  • Silvestri, Vincenzo;Abou-Samra, Ghassan
    • Geomechanics and Engineering
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    • v.1 no.4
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    • pp.263-274
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    • 2009
  • The modified Cam clay (MCC) model is used to study the response of virgin compressed clay in undrained compression. The MCC deviatoric stress-strain relationship is obtained in closed form. Elastic and plastic deviatoric strains are taken into account in the analysis. For the determination of the elastic strain components, both a variable shear modulus and constant shear modulus are considered. Constitutive relationships are applied to the well-known London and Weald clays sheared in undrained compression.