• Korean Journal of Microbiology
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• v.48 no.2
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• pp.79-86
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• 2012

ErmSF is one of the Erm family proteins which catalyze S-adenosyl-$_L$-methionine dependent modification of a specific adenine residue (A2058, E. coli numbering) in bacterial 23S rRNA, thereby conferring resistance to clinically important macrolide, lincosamide and streptogramin B ($MLS_B$) antibiotics. $^{222}FXPXPXVXS^{230}$ (ErmSF numbering) sequence appears to be a consensus sequence among the Erm family. This sequence was supposed to be involved in direct interaction with the target adenine from the structural studies of Erm protein ErmC'. But in DNA methyltarnsferase M. Taq I, this interaction have been identified biochemically and from the complex structure with substrate. Arginine 223 and 227 in this sequence are not conserved among Erm proteins, but because of the basic nature of residues, it was expected to interact with RNA substrates. Two amino acid residues were replaced with Ala by site-directed mutagenesis. Two mutant proteins still maintained its activity in vivo and resistant to the antibiotic erythromycin. Compared to the wild-type ErmSF, R223A and R227A proteins retained about 50% and 88% of activity in vitro, respectively. Even though those arginine residues are not essential in the catalytic step, with their positive charge they may play an important role for RNA binding.

• Journal of The Korean Association For Science Education
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• v.25 no.3
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• pp.400-410
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• 2005

According to the cognitive conflict process model, student anxiety factor is known to have both positive and negative effects on student response behaviors in a conflict situation for conceptual change learning. However, there is little research that reveals what type of anxiety, either constructive or destructive, is related when conducting step-by-step experiments to resolve cognitive conflicts. This study attempted to learn the characteristic of explanatory hypothesis according to anxiety type after conducting five step-by-step experiments related to action and reaction concept. Results found that students who belonged to the types of 'conviction in logical misconception', 'insisting on additional variables', and 'reasonable modification' suggested explanatory hypothesis close to physical nature. On the other hand, those who showed the other five types of anxiety ('compatible predictions', 'dependence on others', 'fusion of past experience', 'lack of confidence', and 'conflict with past experience') suggested temporary supported hypothesis or simple explanatory hypothesis according to student intuition and simple observation. These results indicate that students in the above-mentioned five categories need more external interactions with instructors based on the type of anxiety related to student behavior. In addition, the results present student characteristics which instructors should be more attentive to when using step-by-step experiments to resolve cognitive conflicts.

• Journal of the Korea Concrete Institute
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• v.16 no.2 s.80
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• pp.249-260
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• 2004

The longitudinal steel connection of reinforced concrete bridge column is sometimes practically unavoidable, however the current Korean bridge design specifications have no special provisions about lap-splices of longitudinal steel. This paper reports experimental results of a research program investigating the seismic performance of circular RC bridge columns with respect to longitudinal steel connection detailing. Twenty-one circular column specimens were tested under quasi-static test. The columns with the entire longitudinal steel lap-spliced within plastic hinge region show relatively sudden strength degradation and low ductility than the columns with continuous longitudinal steel and the columns with half of longitudinal steel lap-spliced. However, the seismic performance of the column with mechanically connected longitudinal steel is similar to that of the column with continuous longitudinal steel. The final objectives of this study are to suggest appropriate longitudinal reinforcement connection details for the limited ductility design concept and to provide quantitative reference data and tendency for performance or damage assessment based on the performance levels such as cracking, yielding, collapse, etc. Ultimate displacement/drift ratio, displacement ductility, response modification factor, equivalent viscous damping ratio, residual deformation index, and effective stiffness are investigated and discussed in this paper.

• Journal of Preventive Medicine and Public Health
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• v.42 no.5
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• pp.337-342
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• 2009

Background : Many previous studies have shown that elevated homocysteine in the serum is a well known risk factor for cardiovascular disease and this is associated with other risk factors for cardiovascular disease, but any Korean data on this is limited. Objectives : This study aimed to calculate the prevalence of hyperhomocysteinemia and to analyze the relation between elevated homocysteine and the lifestyle factors of Korean adults. Methods : We conducted a cross-sectional survey that included 650 men and 743 women (age range, 20 to 79 years) who were residents of Gwangju City in Gyeonggi-do. These subjects participated in the health interview and examination survey from November to December 2005. The total homocysteine, total cholesterol, HDL-cholesterol, LDL-cholesterol, and triglyceride in the serum were measured. All the participants had their body composition measured such as height and weight, and we obtained health-related behavioral information through the self-entry questionnaire. Results : Very right-handed skewed distributions of homocysteine were shown in men and women. The prevalence of hyperhomocysteinemia was 22.6% in men and 13.7% in women in Gwangju city. On the multiple logistic regression analysis, hyperhomocysteinemia was associated with age (OR=1.02, 95% CI=1.01-1.04), male gender (OR=1.60, 95% CI=1.02-2.52), severe general physical activity (OR=0.32, 95% CI=0.15-0.69) and nutrient consumption (OR=0.49, 95% CI=0.31-0.76). Conclusions : There is a great prevalence of hyperhomocysteinemia in adults of Gwangju City, Korea and it was associated with both genetic factors and lifestyle risk factors. This study can suggest that comprehensive lifestyle modification is needed in order to diminish the prevalence of hyperhomocysteinemia and to prevent CVD.

• Journal of Internet Computing and Services
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• v.21 no.2
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• pp.81-90
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• 2020

The purpose of this study is to study how to apply block-chain technology to prevent data forgery and alteration in the defense sector of AI(Artificial intelligence). AI is a technology for predicting big data by clustering or classifying it by applying various machine learning methodologies, and military powers including the U.S. have reached the completion stage of technology. If data-based AI's data forgery and modulation occurs, the processing process of the data, even if it is perfect, could be the biggest enemy risk factor, and the falsification and modification of the data can be too easy in the form of hacking. Unexpected attacks could occur if data used by weaponized AI is hacked and manipulated by North Korea. Therefore, a technology that prevents data from being falsified and altered is essential for the use of AI. It is expected that data forgery prevention will solve the problem by applying block-chain, a technology that does not damage data, unless more than half of the connected computers agree, even if a single computer is hacked by a distributed storage of encrypted data as a function of seawater.

• Journal of the HCI Society of Korea
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• v.1 no.1
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• pp.1-8
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• 2006

We suggest a graphical representation of the tension flow in tonal music using a piecewise parametric curve, which is a function of time illustrating the changing degree of tension in a corresponding chord progression. The tension curve can be edited by using conventional curve editing techniques to reharmonize the original music with reflecting the user's demand to control the tension of music. We introduce three different methods to measure the tension of a chord in terms of a specific key, which can be used to represent the tension of the chord numerically. Then, by interpolating the series of numerical tension values, a tension curve is constructed. In this paper, we show the tension curve editing method can be effectively used in several interesting applications: enhancing or weakening the overall feeling of tension in a whole song, the local control of tension in a specific region of music, the progressive transition of tension flow from source to target chord progressions, and natural connection of two songs with maintaining the smoothness of the tension flow. Our work shows the possibility of controlling the perceptual factor (tension) in music by using numerical methods. Most of the computations used in this paper are not expensive so they can be calculated in real time. We think that an interesting application of our method is an interactive modification of tension in background music according to the user's emotion or current scenario in the interactive environments such as games.

• Journal of Radiation Protection and Research
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• v.28 no.4
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• pp.311-319
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• 2003

We developed a grid-based Gaussian plume model to evaluate tracer release data measured at Young Gwang nuclear site in 1996. Downwind distance was divided into every 10m from 0.1km to 20km, and crosswind distance was divided into every 10m centering released point from -5km to 5km. We determined dispersion factors, ${\sigma}_y\;and\;{\sigma}_z$ using Pasquill-Gifford method computed by atmospheric stability. Forecasting ability of the grid-based Gaussian plume model was better at the 3km away from the source than 8km. We confirmed that dispersion band must be modified if receptor is far away from the source, otherwise P-G method is not appropriate to compute diffusion distance and diffusion strength in case of growing distance. So, we developed an empirical equation using linear programming. An objective function was designed to minimize sum of the absolute value between observed and computed values. As a result of application of the modified dispersion equation, prediction ability was improved rather than P-G method.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.35 no.2
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• pp.287-297
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• 2015

In concrete structural design provisons, there is a minimum allowable strain of steel to ensure a ductility of RC members and a c/d is limited for the same purpose in EC2. In general, a ductility capacity of RC members is evaluated by a displacement ductility which is a ratio of ultimate displacement to yield displacement, and it is necessary to calculate accurately a yield displacement and an ultimate displacement to evaluate a displacement ductility. But a displacement in members is affected by various member characteristics, so it is hard to calculate a displacement exactly. In this study, a displacement ductility is calculated by calculating a yield displacement and an ultimate displacement through a moment-curvature relationship. The main variables examined are concrete strength, yield strength, steel ratio, spacing of confinement, axial force ratio and concrete ultimate strain. As results, as a concrete strength is increased, a ductility displacement is increased. But as yield strength, steel ratio, spacing of confinement and axial force ratio are increased, a displacement ductility is decreased. And a displacement ductility is necessary to calculate a response modification factor (R) of columns for seismic design, so it is appeared that it is important to calculate a displacement ductility more accurately.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.2
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• pp.691-694
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• 2014

Background: Tumor length in patients with esophageal cancer (EC) has recently received great attention. However, its prognostic role for EC is controversial. The purpose of our study was to characterize the prognostic value of tumor length in EC patients and offer the optimum cut-off point of tumor length by reliable statistical methods. Materials and Methods: A retrospective analysis was conducted on 71 consecutive patients with EC who underwent surgery. ROC curve analysis was used to determine the optimal cut-off point for tumor length, measured with a handheld ruler after formalin fixation. Correlations between tumor length and other factors were surveyed, and overall survival (OS) rates were compared between the two groups. Potential prognostic factors were evaluated by univariate Kaplan-Meier survival analysis. A P value less than 0.05 was considered significant. Results: There were a total of 71 patients, with a male/female divide of 43/28 and a median age of 59. Characteristics were as follows: squamous/adenocarcinoma, 65/6; median tumor length, 4 (0.9-10); cut-off point for tumor length, 4cm. Univariate analysis prognostic factors were tumor length and modality of therapy. One, three and five year OS rates were 84, 43 and 43% for tumors with ${\leq}4cm$ length, whereas the rates were 75, 9 and 0% for tumors >4 cm. There was a significant association between tumor length and age, sex, weight loss, tumor site, histology, T and N scores, differentiation, stage, modality of therapy and longitudinal margin involvement. Conclusions: Future studies for modification of the EC staging system might consider tumor length too as it is an important prognostic factor. Further assessment with larger prospective datasets and practical methods (such as endoscopy) is needed to establish an optimal cut-off point for tumor length.

• Journal of Korean Society of Forest Science
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• v.97 no.1
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• pp.22-29
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• 2008

The objective of this study is to provide useful information for the establishment of efficient policy implementation strategies of forest road construction policy in South Korea. Data needed for the analysis was collected by a questionnaire survey. For the analysis, policy evaluation model was constructed based on theories of public policy. Evaluation model contains three independent variables (policy initiative factor, policy content, policy environment) and two dependent variables (policy result, policy impact). Since, these variables are unobservable latent variables, observable indicators are needed as proxy measures. LISREL (Linear Structural Relationships) was employed for the analysis since it is a useful measure for analysing linear structural model which consists of structural and measurement equations. It was confirmed that forest road construction is an effective policy mean for the development of rural region and activating forest resources management. The policy outcome, however, was not satisfactory. To improve the effectiveness of forest road construction policy some modification of policy contents are needed such as increased construction budget, allowing more flexibility and participation to the implementation personal and providing technical support.