• Biomolecules & Therapeutics
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• v.24 no.1
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• pp.19-24
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• 2016

Acute myocardial infarction (AMI) remains a leading cause of morbidity and mortality worldwide. The exploration of new biomarkers with high sensitivity and specificity for early diagnosis of AMI therefore becomes one of the primary task. In the current study, we aim to detect whether there is any heart specific long noncoding RNA (lncRNA) releasing into the circulation during AMI, and explore its function in the neonatal rat cardiac myocytes injury induced by $H_2O_2$. Our results revealed that the cardiac-specific lncRNA MHRT (Myosin Heavy Chain Associated RNA Transcripts) was significantly elevated in the blood from AMI patients compared with the healthy control ($^*p<0.05$). Using an in vitro neonatal rat cardiac myocytes injury model, we demonstrated that lncRNA MHRT was upregulated in the cardiac myocytes after treatment with hydrogen peroxide ($H_2O_2$) via real-time RT-PCR (qRT-PCR). Furthermore, we knockdowned the MHRT gene by siRNA to confirm its roles in the $H_2O_2$-induced cardiac cell apoptosis, and found that knockdown of MHRT led to significant more apoptotic cells than the non-target control ($^{**}p<0.01$), indicating that the lncRNA MHRT is a protective factor for cardiomyocyte and the plasma concentration of MHRT may serve as a biomarker for myocardial infarction diagnosis in humans AMI.

• Journal of Korean Society on Water Environment
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• v.33 no.6
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• pp.626-639
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• 2017

Based on an ecological monitoring in a Korean stream (Gapyeong), Habitat Suitability Index (HSI) of nine fish species was developed for three physical habitat factors : current velocity, water depth and substrate. The species were chosen based on their abundance and frequency in the fish community of the Gapyeong stream. The Weibull model was used as the probability density function to analyze the distribution and number of each fish species according to the three identified physical factors, which showed good results. This HSI equation has advantages because it statistically expresses habitat preferences of fish species simply and clearly. From that, we can quantitatively deduce the central tendency and variation of environmental factors for fish distribution. The selected fish species showed different preferences for each habitat factor respectively. Although there are some exceptions, the distribution and abundance of individual species of nektonic fish (Zacco koreanus, Zacco platypus, Microphysogobio longidorsalis and Pungtungia herzi) were positively skewed to deep water and fine substrate while riffle-benthic fish (Koreocobitis rotundicaudata and Coreoleuciscus splendidus) were normally distributed at the shallow and coarse substrate zone. It seems that the species showing the positively skewed distribution to the current, Z. koreanus, Z. platypus, M. longidorsalis and P. herzi have adapted themselves to the fast current and have expanded their niche.

• Journal of the Korean Wood Science and Technology
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• v.48 no.4
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• pp.548-561
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• 2020

Paecilomyces japonica is widely cultured to produce mycelium for medicinal and health food use. Illumination is an important factor in the growth and production of mycelium in submerged culture. The effects of different light-emitting diode (LED) combinations on the growth and cordycepin content as bioactive substances of mycelium were investigated. The results showed that the mycelium dry weights were lower under dark condition and red LED treatments. Dark condition, fluorescent light, and ultraviolet-A failed to increase the cordycepin content. Blue light was necessary to increase the cordycepin content, and a red-to-blue ratio of 3:7 induced the highest cordycepin content. The cordycepin contents of mycelium in submerged culture were significantly higher in a 12 h/day illumination time under red and blue (red-to-blue ratio of 3:7) LED treatments, showing an increase of up to 38% compared with those under the fluorescent-light control condition. The results demonstrated the roles of light with different wavelengths on the biosynthesis of cordycepin as bioactive substances. The low-heat release and replacement of traditional fluorescent lights with low-energy-consuming LEDs could increase the contents of bioactive substances. After optimization of the cordycepin production using response surface methodology (Box-Behnken design) to its canonical form, the optimum combination was found to be as follows: illumination time = 17.7 h/day, sugar content in the medium = 9.7 g/50 mL, and incubation time = 61.2 h. The model predicted a maximum response of 3779.2 ㎍/mL cordycepin yield.

• Journal of Korea Water Resources Association
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• v.33 no.3
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• pp.279-288
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• 2000

The impact of land slope to the degree of flow divergence was considered employing distributional applications of slope exponents in the now directlOn algoriUnns. Lmear, exponential and ]X)wer law of distributional functIons were employed to address the variation of slope exponents m a terrain analysis. Dongok subwatershed at Wichun test watershed was selected as a study area. Digital Elevation Models of 20m, 30m, 40m and 50m grid size were made to perfonn the analysis. Various calcualtion methodologies of topographic index and the impact of grid sizes were investigated in terms of statistical and spatial aspects. DIstributional applications of slope e.xponents made it possible to represent the flow divergence and convergence about the ten-ain characteristics. The Monte~Carlo method was used to simulate six runoff events to check the impact of topographic factor in the runoff simulation.

• Korean Medical Education Review
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• v.12 no.1
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• pp.23-31
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• 2010

Medical humanities has become a third area of medical education following basic and clinical medicine. Also, in the national evaluation of medical schools, medical humanities education is an important factor. However, there are many difficulties in teaching medical humanities in medical schools. First, it is still an unfamiliar education area to medical schools and professors. Second, still, there is no consensus on the definition and contents of this education. Third, it is usually very difficult to find professors who have interest and the ability to teach medical humanities. Fourth, even medical students do not understand why they should study medical humanities and sometimes do not eagerly participate in class. This paper suggests some solutions for these problems. First, medical humanities need to be divided into sections according to how easily the contents can be accepted by existing medical education system and apply these sections in the introduction of this education gradually and in stage. One example of the division can be as follows: Group 1) medical ethics and medical law which can be most easily accepted. Group 2) medical communication skills which can be relatively easily accepted. Group 3) medical history and medical professionalism which is relatively difficult to accept, and Group 4) medical philosophy, medicine and music, medicine and literature, medicine and art, medicine and religion, etc. which is the most difficult to accept. In this paper, four things are suggested. Second, divide the contents into mendatory courses and elective courses. Third, allocate the contents throughout the four years from the first year though the fourth year according to the spiral curriculum model. This paper reports some new ideas and methods for medical humanities education. First, to stimulate students' participation, several methods were applied in a large size lecture and student projects. Second, the emphasis of writing in class and evaluation were discussed. Third, the provision of hands on experience is more emphasized than lectures. Fourth, inviting some doctors who work in non-medical areas such as journalism, pharmaceutical industry, etc is suggested. Trial and error is inevitable in this education, but it is essential in molding a good doctor, so medical professors who are interested or in charge of this medical humanities education need to share their ideas and experiences.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2012.10a
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• pp.743-749
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• 2012

Grunt (Stick-slip) noise happens between rear lining and drum on braking condition while vehicle is returning to steady position after nose-dive. The study presents a new testing and analysis methods for improving brake grunt noise on vehicle. Grunt noise is called a kind of stick slip noise with below 1kHz frequency that is caused by the surfaces alternating between sticking to each other and sliding over each other with a corresponding change in friction force. This noise is typically come from that the static friction coefficient of surfaces is much higher than the kinetic friction coefficient. For the identification of the excitation mechanism and improvement of grunt noise, it is necessary to study variable parameters of rear drum brake systems on vehicle and to implement CAE analysis with stick slip model of drum brake. The aim of this study has been to find solution parameters throughout test result on vehicle and dynamo test. As a result of this study, it is generated from stick slip between rear lining and rear drum and it can be solved to reduce contact angle of lining with asymmetric and is effected not only brake drum strength but also rear brake size and brake factor.

• The Journal of Korean Society for School & Community Health Education
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• v.14 no.3
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• pp.1-14
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• 2013

Objectives: The purpose of this study was to investigate the possible impact factors(oral health level, oral health promotion behaviors, health level, health behaviors, and mental health) on oral health related quality of life using OHIP-14 of health allied college students. Methods: A total of 363 self-administered questionnaires were collected from university student in Seoul. To investigate the casual relationship between each variable presented in the research model, descriptive statistics, t-test, one-way ANOVA(Scheffe's test), man-whitney, kruskal wallis, multiple regression analysis were carried out by using SPSS ver. 21.0 Results: The study shows that the students reported mean score of OHIP-14 ($8.32{\pm}7.51$), of which physical pain was the highest score($1.88{\pm}1.45$) and social disability was the lowest score($0.69{\pm}1.13$). Multiple regression revealed that the score of OHIP-14 was shown to be significantly higher for the following people: who were get more self-reported symptom of periodontitis, halitosis, negative self-perceived general health and oral health, no received dental scaling treatment, and participants who had no experience awareness of distress in two weeks. The explanatory power was 18.2%. The most powerful factor regarding to self-reported symptom of periodontitis was shown to be negatively relations oral health-related quality of life. Conclusions: In order to enhance the students' life quality, there need to be considered for a comprehensive oral health-related quality of life program for the students through health education policy.

• Journal of the Korean Society of School Health
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• v.28 no.2
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• pp.56-66
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• 2015

The purpose of this study was to investigate factors associated with coping responses in Korean public high school students. Methods: This study employed a descriptive survey research design. The sample included 263 high school students who responded to a self-report questionnaire. Variables included socio-demographic characteristics, health-related characteristics, academic stress, somatization symptoms, social support, and coping responses. Data were analyzed using descriptive statistics, t-tests, ANOVA, Pearson's correlations, and multiple regressions. Results: Participants, regarding their school life, reported moderate levels of academic stress ($M{\pm}SD=2.3{\pm}0.52$) and somatization symptoms ($M{\pm}SD=2.3{\pm}0.71$), and a relatively high level of social support ($M{\pm}SD=4.2{\pm}0.67$). All the variables were associated with the use of multiple coping responses. Active-cognitive coping ($M{\pm}SD=2.9{\pm}0.68$) was most frequently used, followed by active-behavioral coping ($M{\pm}SD=2.5{\pm}0.56$). and avoidant coping ($M{\pm}SD=2.3{\pm}0.75$). Significant relationships were found among the measured variables: positive relation between academic stress and somatization symptoms, but, negative between academic stress and both somatization symptoms and social support. Students who had higher stress and more somatization symptoms were more likely to use avoidant coping than the others. In multiple regression analysis, while factors associated with each coping response differed, gender appeared to be a significant factor in all methods. Variables included in the final model explained 27% of the variance in avoidant coping (F=11.40, p<.001). Conclusion: Based on the study results, schools should provide tailored educational programs to help high school students reduce multisource stress and somatization symptoms at school and cope with them in more active and effective ways.

• Proceedings of the Korean Vacuum Society Conference
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• 2011.02a
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• pp.21-21
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• 2011

A series of Quasi-Elastic Neutron Scattering (QENS) experiments helps us to understand the single-particle (hydrogen atom) dynamics of a globular protein and its hydration water and strong coupling between them. We also performed Molecular Dynamics (MD) simulations on a realistic model of the hydrated hen-egg Lysozyme powder having two proteins in the periodic box. We found the existence of a Fragile-to-Strong dynamic Crossover (FSC) phenomenon in hydration water around a protein occurring at TL=$225{\pm}5K$ by analyzing Intermediate Scattering Function (ISF). On lowering of the temperature toward FSC, the structure of hydration water makes a transition from predominantly the High Density Liquid (HDL) form, a more fluid state, to predominantly the Low Density Liquid (LDL) form, a less fluid state, derived from the existence of a liquid?liquid critical point at an elevated pressure. We showed experimentally and confirmed theoretically that this sudden switch in the mobility of the hydration water around a protein triggers the dynamic transition (so-called glass transition) of the protein, at a temperature TD=220 K. Mean Square Displacement (MSD) is the important factor to show that the FSC is the key to the strong coupling between a protein and its hydration water by suggesting TL${\fallingdotseq}$TD. MD simulations with TIP4P force field for water were performed to understand hydration level dependency of the FSC temperature. We added water molecules to increase hydration level of the protein hydration water, from 0.30, 0.45, 0.60 and 1.00 (1.00 is the bulk water). These confirm the existence of the FSC and the hydration level dependence of the FSC temperature: FSC temperature is decreased upon increasing hydration level. We compared the hydration water around Lysozyme, B-DNA and RNA. Similarity among those suggests that the FSC and this coupling be universal for globular proteins, biopolymers.

• Journal of Korea Water Resources Association
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• v.34 no.3
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• pp.231-240
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• 2001

The problem of the flocculation basin was induced by installing the pilot plant using tracer test at Yang-Duck water treatment plant in Pohang cite. The flocculation basin model downscaled as 1/20 was made of acryl and evaluated hydraulically by transforming the section of the effluent in the flocculation basin. The optimum section of the effluent was suggested and applied to the plant. The efficiency of the flocculation basin according to improvement was evaluated by the particle counter which can count the number of particles each size fraction. The results of this study are as follows : First, it was desirable to make the retention time as short as possible because the flow and the index value were similar regardless of the retention time. Second, after the modification in Yang-Duck water treatment plant, the problem of destruction of floe was improved and the plant was operated satisfactorily. The hydraulic experiment with tracer test can be applied to the performance evaluation as well as improvement of facility in unit process of existing water treatment plant. Additionally, it can be used to find a design factor in the new water treatment plant.